Alfalfa weevil, Hypera postica Gyllenhal, is regarded as one of the most important insect pests on Chinese milk vetch and hairy vetch. The insect usually feed on leaves, stems and pods of the green manure which causes severe reduction of biomass. This study was conducted to observe the occurrence of H. postica larva and adult on Chinese milk vetch in 2009 to 2010, and to evaluate host plants preference of 11 different seedlings in 2010, Milyang, Korea. Occurrence of H. postica larvae on Chinese milk vetch was observed to increase from early April clearly and its peak population was showed during middle to late April. The 1st generation of newly emerged adult of H. postica was appeared on 20th May. Newly emerged H. postica adult was fed on green manure such as Chinese milk vetch and hairy vetch as well as various seedlings located at nearby upland. And then the insects were migrated into aestivating places in middle to late of June. Thus, host plant preference of H. postica adults were evaluated with 11 different seedlings using transparent acrylcage and at Chinese milk vetch field. As a result, H. postica adult preferred on Chinese cabbage, followed by soybean, kale, foxtail millet and proso millet. The damaged rating of seedlings were shown to have the same trend with adult occurrence. In particular, few H. postica adults were occurred on eggplant, lettuce, corn, crown daisy and peanut, and thereby those crops were regarded as non-preference plants due to the absence of damage. These informations can be used by upland farmers who sowing from late May to early June for effective management of H. postica.

This study was conducted to determine whether trichome density affects the oviposition behavior of adult female Tetranychus urticae Koch on host plant leaves. Experiments were conducted with twenty replications on the leaf discs of each plant (Pear, 'Niitaka'; Apple, 'Fuji'; Strawberry, 'Meahyang'; 3cm diameter) at 25℃, 60-70% RH and a photoperiod of 16:8 (L:D) h. One female T. urticae was placed on each disc. The number and locations of laid eggs were recorded at 24 h intervals until T. urticae died. The trichomes were distributed along the midrib of abaxial surface of pear leaves but were evenly distributed on that of apple and strawberry leaves. Eggs were mostly laid along the midrib of pear leaf disc, but eggs were laid not only along the midrib but also randomly over the leaf disc of apple and strawberry. Therefore, it appeared that T. urticae preferred to lay eggs on the specific location where trichomes were densely distributed. Further study is needed to quantify how different distribution patterns of T. urticae eggs on different plant leaves affect the efficacy of predatory mites to control T. urticae.

This study demonstrates a variability in efficacy of mating disruption against the oriental fruit moth, Grapholita molesta, populations infesting apples cultivated in differential environmental conditions. Throughout the growing seasons, trap catches of G. molesta male moths and damaged leaf and fruit were examined to evaluate the efficacy of a commercialized mating disruptor. Treatment of the mating disruptor significantly reduced the trap catches of male moth in treated plot, compared to those of the untreated plot. This reduced trap catches were significantly correlated with leaf and fruit damage. Moreover, the highest host damage occurred in June just after the highest overwintering adult peaks in both plots. The treatment of mating disruptors in Chungsong effectively disrupted the overwintering population in April and May, resulting in no noticeable host damage during the following growing seasons. However, there was a marked difference in host damage between two plots, especially in late seasons. Variation in the efficacy of mating disruption technology in terms of host damage appeared to be related with nearby pheromone-untreated orchards, which may result in the immigration of gravid females.

광대노련재의 계대사육법을 확립하기 위해 , 605% R.H., 16L.8D광주기조건 의 실내 사육환경하에서 대체먹이인 생땅콩을 급여하여 사육하면서 발육 특성과 기주 및 산란선 호성을 조사하였다. 광대노련재의 1세대 소요일수는 평균 48일이었다. 알은 유백색의 구형이고 1 난괴당 산란수는 14개가 대부분이었다. 알.약충의 발육기간을 조사한 결과 알기간은 8.20.4일 이었고 각 령별 약충기간은 1령 5.40.2일,2령 8.40.5일, 3령 6.40.4일,4령 7.40.3일,5령 12.60.8일이였다. 성충의 생존일수는 수컷이 평균 30.8일, 암컷은 35.4일 정도였다. 성충화율은 5 57.1%였는데 암수비융은 47: 53으로 수컷의 비율이 약간 높았다. 산란전기간은 평균 22.5일이었 고 펑균산란회수는 4.4회였다. 산란선호성은 기주식물이 있을 경우에는 기주에 산란하지만 기주식 물을 제외시킨 경우에는 아크릴 사육상자, 철망보다는 인조잎에 더 많이 산란하였다. 광대노린재 성충의 기주선호성을 조사한 결과 대상식물 18종 중 황벽나무, 산초나무, 산수유, 충층나무 등의 줄기 및 과실을 가장 잘 흡즙하는 것으로 나타났다.

담배가루이(B biotype)의 온도조건과 기주별로 발육과 생식에 미치는 영향을 조사하였다. 알에서 성충까지의 발육기간은 에서 86.2일이었고, 에서는 17.0일로 온도가 높아질수록 약 5배 짧았다. 알, 약충 그리고 알에서 성충까지의 발육영점온도는 각각 , , 였고, 유효적산온도는 각각 110.3, 204.7, 317.3일도였다. 부화율은 에서 87.0%, 우화율(부화유충에서 성충까지)은 에서 76.7%로 높았다. 성충수명은 에서 23.6일이었고, 에서는 14.0일로 온도가 높을수록 짧았다. 그리고 암컷한마리당 평균 총산란수는 에서 103.3개로 가장 많았으나 온도간에 유의성은 없었다. 1세대당 순증식율()은 에서 97.33로 가장 높았다. 그리고 내적자연증가율()은 온도가 높아 질수록 높아졌으며, 에서 0.196으로 가장 높았다. 토마토, 고추, 가지, 포인세치아를 기주로 조건에서 알에서 우화까지의 발육기간은 각각 21.2, 28.1, 22.2, 25.5일이었다. 부화율은 고추에서 90.3%로 가장 높았고, 우화율은 가지에서 89.6%로 가장 높았다. 성충수명은 가지에서 26.5일로 가장 길었고, 고추에서 가장 짧았다. 암컷 한마리당 평균 산란수는 토마토와 가지에서 많았다. 1세대당 순증식율()은 가지에서 106.1로 가장 높았고, 내적자연증가율()은 토마토에서 0.165로 가장 높았다. 이상의 결과로 담배가루이의 성장에 적합한 온도범위는 이었고, 가장 적합한 기주식물은 토마토와 가지로 분석되었다.

Fungus gnats, Bradysia spp.의 기주를 조사한 결과 오이, 멜론, 수박, 애호박, 토마토, 고추, 파프리카, 백합, 카네이션, 장미, 거베라, 국화, 호접란, 글라디오러스, 생강, 천궁, 작두콩, 신선초, 등굴레, 소나무, 해송 등 21종이 조사되었고 이들 유충은 작물의 지제부나 뿌리근권을 가해하여 뿌리절단, 줄기내 침입 등으로 생육저해, 시들음 증상, 식물체 고사의 원인이 된다.

Insects are among the most important abiotic and biotic constraints to rice production. National rice research programs are in various stages in the development and implementation of integrated pest management (IPM) stratagies for rice insect control. Among the various control tactics, insect resistant cultivars are sought as the major tactic in rice IPM. Through the activities of interdisciplinary teams of scientists significant progress has been made in the development and release of insect resistant cultivars to farmers. Because of its compatibility with other control tactics insect resistance has proven to fit well into the IPM approach to rice insect control agents and minimize the need for insecticide applications. The development of biotypes which overcome the resistance in rice plants has been a significant constraint in the breeding of rice for resistance to insects. Most notable examples in Asia are the green leafhopper, Nephotettix virescens, brown planthopper, Nilaparvata lygens and the Asian rice gall midge, Orseolia oryzae. The current breeding stratege is to develop rice cultivars with durable resistance on which virulent biotypes cannot adapt. In spite of the significant progress made in the breeding of insect resistant cultivars there are still numerous important rice insect species for which host plant resistance as a control tactic has not been fully utilized. Advances in biotechnology provide promise of solving some of the problems that have limited the use of host plant resistance as a major tactic in the integrated management of rice insect pests.

전남지역(全南地域) 대나무 집단재배지(集團栽培地)에 피해(被害)가 확대(擴大)되고 있는 잎말이나방류(類)의 발생(發生), 기주식물(寄主植物), 생태(生態) 및 피해(被害)에 관(關)해서 조사(調査)한 바 다음과 같은 결과(結果)를 얻었다. 1. 전남지역(全南地域)에서 대나무엽(葉)에 피해(被害)를 주는 명나방류(類)에는 줄노랑명나방(Microstega jessica (B.))과 줄허리들명나방(Sinibotys evenoralis (W.) 2종(種)이었고 담양(潭陽)에서 발생비율(發生比率)을 보면 줄노랑명나방이 96.5%, 줄허리들명나방이 3.5%이었으나 나주(羅州), 화순(和順)에서는 줄허리들명나방만이 발생(發生)되었다. 2. 기주식물(寄主植物) 조사(調査)에서 줄노랑명나방은 왕대(Phyllostachys bambusoides S. et Z.), 죽순대(P. pubescens Mazel), 솜대 (P. nigra var. henonis Stapf), 오죽(P. nigra Munro), 줄허리들명나방은 왕대, 솜대, 오죽으로 밝혀졌는데, 본조사(本調査)에서는 줄노랑명나방의 솜대와 오죽 2종(種)과 줄허리들명나방의 솜대 1종(種)이 새로운 기주(寄主)로서 확인(確認)되었다. 3. 줄노랑명나방은 담양지역(潭陽地域)에서 연(年) 1회(回) 발생(發生)하며 그 최성기(最盛期)는 5월(月)하순(下旬)이었고 줄허리들명나방은 나주지역(羅州地域)에서 연(年) 2회(回) 발생(發生)으로 그 최성기(最盛期)는 6월(月)상순(上旬)과 7월(月)중순(中旬) 경(頃)이었다. 4. 각(各) 태별(態別) 평균기간(平均期間)을 보면 줄노랑명나방은 난(卵)이 8일(日), 유충이 293일(日), 용(踊)이 16일(日), 성충수명은 자충이 12일(日), 웅충이 9일(日)이었고, 줄허리들명나방은 1화기(化期)는 난(卵)이 11일(日), 유충이 28일(日), 용(踊)이 12일(日) 성충수명은 자충이 18일(日), 웅충이 15일(日)이었으며 2화기(化期)는 난(卵)이 7일(日), 유충이 261일(日) 용(踊)이 20일(日), 성충수명은 자충이 11일(日) 웅충이 10일(日)이었다. 5. 1일중(日中) 줄노랑명나방의 성충우화시간을 보면 저녁 6시(時)부터 새벽 4시(時)까지였고 그 Peak는 밤 시(時)였다. 6. 월동태(越冬態) 및 월동장소(越冬場所)는 줄노랑명나방은 6령충(齡蟲)으로 땅에 떨어진 죽순껍질과 새로 나온 대나무 밑부분에 붙어있는 죽순껍질에서 줄허리들명나방은 령충으로 수상(樹上) 대나무엽(葉)에서 월동(越冬)하였다. 7. 줄노랑명나방의 수고별(樹高別) 피해엽율(被害葉率)에 있어서는 주(主)로 상부(上部)에 피해(被害)가 많았으며 하부(下部)로 내려갈수록 피해(被害)가 적었다. 종류별(種類別)로는 솜대, 왕대 죽순대 순(順)으로 피해(被害)가 많았으며 수령(樹齡)과 피해율(被害率)과의 관계(關係)는 1년생(年生), 2년생(年生), 3년생(年生), 4년생(年生), 5년생(年生) 순(順)으로 부(負)의 상관관계(相關關係)()가 인정(認定)되었다.

전남지방(全南地方)의 주요(主要) 과수(果樹) 수종(樹種)인 배, 자두, 복숭아, 사과, 포도의 성숙기(成熟期)에 피해(被害)가 큰 과실(果實) 흡수(吸收)나방류(類)의 분류(分類), 유충(幼蟲)의 기주범위(寄主範圍) 및 피해(被害)에 관(關)하여 1982년(年)부터 1983년(年) 2개년(個年)에 걸쳐 조사(調査)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 본(本) 조사(調査)에서 분류(分類)된 흡수(吸收)나방류(類)는 총(總) 31종(種)이었으며 이 중(中) 회색붉은뒷날개나방, 우단태극나방, 왕흰줄태극나방, 스투포사밤나방, 박각시나방, 세줄박각시나방, 분홍등줄박각시나방(복숭아박각시나방), 무늬뾰쪽날개나방, 흰제 비불나방, 곧은띠수염나방 등(等) 10종(種)이 새로이 밝혀졌다. 2. 과수원(果樹園) 주위(周圍)에 분포(分布)된 흡수(吸收)나방류(類) 유충(幼蟲)의 주요(主要)한 기주식물(寄主植物)로는 청미래덩굴, 자귀나무, 댕댕이덩굴, 꿩의다리, 검양옻나무, 개굴피나무, 가래나무, 나무딸기, 가지, 배추 등(等)이었다. 3. 주요(主要) 과수(果樹)의 품종별(品種別) 피해(被害)를 보면 자두는 Santa Rosa>Beauty>Soldam, 복숭아에서는 조생백도(早生白桃)황도(黃桃)>백도(白桃), 사과는 축(祝)>홍옥(紅玉), 포도는 Campbell Early>Neo Muscat>Golden Queen 순(順)으로 피해(被害)가 컸다. 4. 흡수(吸收)나방류(類)의 발생량(發生量)과 주요(主要) 과수(果樹)의 숙기(熟期)와의 관계(關係)를 보면 자두와 복숭아 숙기(熟期)인 7월(月) 초순(初旬)하순경(下旬頃)과 사과 및 포도의 숙기(熟期)인 8월(月) 하순(下旬)9월(月) 중순경(中旬頃)에 발생량(發生量)이 많았다. 5. 주요(主要) 과수별(果樹別) 가장 피해(被害)를 많이 주고 있는 흡수(吸收)나방류(類)는 자두의 경우 무궁화밤나방, 으름밤나방, 복숭아에서 태극나방, 작은갈고리밤나방, 포도에 작은갈고리밤나방, 태극나방, 그리고 사과에서는 태극나방이었다. 6. 흡수(吸收)나방류(類)의 비래시간(飛來時間)은 일몰후(日沒後) 6시(時)부터 시작(始作)되어 새벽 2시(時)까지였으며 그 Peak 는 밤 1012시(時)였다.

강남콩 렉틴과 근류균(R. phaseoli)의 결합에 관한 광범위한 실험을 수행하여 다음과 같은 결과를 얻었다. 1. 강남콩종자의 자엽에서 가장 많은 렉틴은 성장에 따라 뿌리로 이동하였는데 발아후 약 5일경에, 뿌리의 길이가 인 것이 가장 렉틴의 함량이 많았다. 2. 근류균의 배양시기에 따가 강남콩렉틴과의 응집력을 측정 한 결과 배양초기 가 후기보다 응집력이 높았다. 3. 강낭콩종자에 존재하는 렉틴을 추출 정제하여서 6종 근류균과의 응집력을 측정한 결과 R. phaseoli와의 응집이 가장 강했고 이는 렉틴에 대한 특이적 결합, 즉 숙주특이성을 입증하는 것이었다. 4. 단당류에 의한 응집반응의 경쟁적 저해를 시도함으로써 강남콩렉틴의 근류균에 대한 결합부위가 mannose와 galactose를 포함한 oligosaccharides가는 것을 알수 있었으며 이들의 저해효과에 필요한 당의 최소농도는 6.25mM로서 측정되었다. 5. R. phaseoli와 강낭콩 렉틴을 강낭콩 뿌리에 인공감염시켜본 결과 렉틴의 경쟁적 저해가 확인되었으므로 뿌리혹형성에 렉틴의 가교가 관여한다는 가설을 입증할 수 있었다. 6. Immunodiffusion에 의한 R. phaseoli의 항원 결정기는 R. japonicum과 일부 관련성이 있을뿐 다른 Rhizobium spp.과는 전혀 다른 것으로 나타났다.

The author studied on the bionomics of Oriental moth. Cnidocampa flavescens WALKER, damaging to the persimmon tree n the southern part of Korea from 1964 to 1965. The results can be summarized as follows, 1. Emergence peak period of Oriental moth was mid-June in Taegu district and eggs are deposited on the opposite side of persimmon tree leaf. Specially most of eggs are deposited on the terminal part of opposite side and peak period s also mid-June. 2. Hatched Percentage of eggs was in 1964 while in 1965 at the rearing room. Mean egg Period was in 1964 while days in 1965. Thus during two years, the egg period was about 6 days. 3. In the growth ratio of Oriental moth fed on various host plants persimmon tree, Acer negund, Hazel-wood and Platanus, the best growth ratio was shown on the leaf of Hazel-wood from 1st till 3rd instar, but, on the contrary, persimmon tree was the best from 4th till the last instar. The growth ratio of head width was also the same tendency as the body length above mentioned. Individuals fed on the leaf of platanus were dead after 20 days. 4. Oriental moth has one generation a year and molts 6 times. The first molting occurred in 5 hours after hatched, and the other moltings were done at f days intervals. After 3 days since the last molting, larvae made the non for over-winter in it. 5. As the bristles on the process of larval body are different from each position and instar, judgement of instars are possible by the counting of bristles on the body according to the Table 8. Specially the bristle of L. 2., D. 2, 3 ,8. 10. and L. 1, 3, 4, 5, 6, 7, are perfectly different from each instar. From these bristles, instars can be recognized easily. 6. Pupation of larvae in the over-wintered cocoon on the stem of persimmon tree was done in mid-May and continued will early June when emergence will take place. 7. Mean number of eggs in the ovary was

Research has found that the management of the host plant is essential to mistletoe cultivation. A Trunk injection test on the host plant that contained a mixture of indole-3-butryc acid (IBA) and liquid fertilizer was conducted with respect to the improvement of the one year survival rate of mistletoe. As a result, the trunk injection experiments showed the effect of the IBA and liquid fertilizer mixture in all treatments. This mixture was effective to increase the survival rate of mistletoe by 20% with the IBA at 100 ㎎/L and Hyponex at 100 ㎎/L. The examination proved that the host plant fertilizer effect was the most effective treatment for organic fertilizer with 60% added NPK (4-2-1). Its effects were higher compared to the control at the length and diameter of one-years-old branches in the host plant, even when the parasitic mistletoe improved its growth in length, diameter, and number of branches. Comparing the control and host plant fertilizer, the latter was the most effective way to process 20 ㎏ per a treatment effect in the experimental site and to process at any time after the inoculation. This treatment is effective to improve the growth of mistletoe by watering the host plant three times per week. Therefore, the management of the host plant is an essential element in the successful cultivation of mistletoe, not only to supply fertilizer and plant hormones to the host plant in the initial inoculation time but also to provide organic fertilizer and irrigation for the host plant.

Metcalfa pruinosa was considered to be a very harmful invasive species, due to its high species density in nature and wide range of its host plants. M. pruinosa was distributed in 28 sites among 143 sites. M. pruinosa has wide range and diverse host plants of 52 families 110 species including crop, fruits and forest trees. At present, the identified host plant of M. pruinosa are composed of 62 families and 145 species in total since their first appearance was reported. M. pruinosa was found in roadside 46.7%, followed by forests 33.3% and orchard 20%. Nymphs and adults cause damage to crop and orchard by sucking juice, outbreak of fungi through secretion of wax, and reduction of plant assimilation due to the nectar of nymphs. Also, it reduces the merchantable quality of fruits and thus causes economic damage. It is judged that M. pruinosa has been moved along major road via the traffic vehicles.

Small RNAs including microRNAs (miRNAs) and small interfering RNAs (siRNAs) play crucial roles in post-transcriptional gene silencing (PTGS) in eukaryotes. Small RNAs function cell-autonomously as well as non-cell-autonomously. It has been well characterized that pathogenic fungi secrete some effector molecules, which facilitate their infection into plants. However, it is not clear whether molecules in plant cells are able to move into fungal cells during infection. To test if small RNAs generated from plant cells can also move to fungal cells during infection, we generated transgenic Arabidopsis and rice plants ectopically expressing either double-stranded RNA interference (dsRNAi) or artificial miRNA (amiRNA) constructs targeting GFP gene. And then these transgenic plants were inoculated with transgenic rice blast fungus, Magnaporthe oryzae, expressing GFP transgene. Here, we showed that ectopic expression of both dsRNAi and amiRNA targeting GFP gene in transgenic plants significantly suppressed GFP expression in rice blast fungi inoculated, indicating that small RNA molecules generated in plant cells can move into infected fungal cells and efficiently degrade fungal GFP transcripts. Our results would provide a new small RNA-based strategy for the development of resistant crops against fungal pathogens.