현재 인간이 일반적으로 이용하는 의료용 단백질은 대개 동물세포 배양기술에 의하여 생산되고 있다. 그러나 제한된 특정 시설에서 생산되기 때문에 생산 단가가 높아서 전세계의 수요가 증가함에도 불구하고 일반화하는 데는 한계가 있는 실정이다. 이러한 문제를 극복하기 위하여 오래전부터 식물시스템을 이용하여 의료용 단백질을 생산하는 연구가 관심을 끌고 있다. 즉 쉽게 규모화할 수 있고 생산비용 효과도 경제적이고 동물세포보다 더 안전하게 의료용 단백질 생산할 수 있는 수단이 될 수 있기 때문이다. 이를 위하여 식물에 안정적으로 유전자를 핵 게놈과 엽록체 게놈에 형질전환 시키는 기술과 바이러스 운반체를 이용하여 일시적 발현을 유도함으로 의료용 단백질을 생산하는 기술이 개발되고 있다. 최근 해체 바이러스 기반의 운반체 개발은 재조합 단백질을 빠르고 일시적 발현으로 대량생산을 가능하게 하고 있다. 따라서 이 바이러스 발현시스템이 적절한 식물기반의 대량생산을 위한 플렛폼을 제공하고 있다. 따라서 본 총설은 식물로부터 의료용 단백질을 생산하는데 있어서 식물 바이러스발현 시스템 개발 및 이용에 대하여 기술하였다.

Among various manual materials handling tasks, pushing/pulling was known to be one of the risk factors for the low back and shoulder musculoskeletal disorders (MSDs). This study was conducted to find out an optimal solution set of the handle height and distance for 4-wheel cart with two vertical handles. Ten male college students participated in the pushing force measurement experiment. The face-centered cube design, one of the central composite designs, was applied for the experiment, and the isometric voluntary pushing force was measured in 9 treatment conditions. The second order response surface model was predicted by using the pushing strength as a response variable, and the handle height and distance as independent factors. According to the 2nd order response model, the handle height and distance showed nonlinear relationship with the isometric pushing strength. To maximize the 2nd order response model (pushing force), the handle height and distance were optimized. The optimal handle height was 'xyphoid process height - stature’, and the optimal handle distance was ‘1.25*shoulder width’. When calculated using the anthropometric data of the subjects of this study, the optimal handle height was 115.4 ± 3.4 cm, slightly higher than the elbow height, and the handle distance was 52.9 ± 2.3 cm.

The objective of this study was to develop of semen transport system for cryopreservation and fertility in bull sperm. The ejaculated semen were diluted with Triladyl containing 20% egg-yolk for transportation. Diluted semen was transported by three methods that there were wrapping tissue (Tissue), sinking under 30℃ water (Water) and sinking between warm water and air (Air) methods. Semen was transported within 2 hours in 0.3℃. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk. And frozen-thawed sperm were estimated with SYBR14/PI double stain for viability, FITC-PNA/PI double stain for acrosome reaction analysis and Rhodamine123 double stain for mitochondrial intact assessment. In results, live sperm (SYBR+/PI-) in Air treatment group (43.3±4.7%) was significantly (p<0.05) higher than other treatment groups (Tissue: 16.3±2.7% and Water: 27.5± 3.1%), dying sperm (SYBR+/PI+) in Air treatment group (55.6±4.7%) was significantly lower than other treatment groups (Tissue: 77.6±3.2% and Water: 67.6±3.3%) (p<0.05). Acrosome reaction in Air treatment group (0.2±0.1%) within live sperm (PI negative region) was significantly (p<0.05) lower than other treatment groups (Tissue: 0.7±0.2% and Water: 0.5±0.1%), the acrosome reaction in Air treatment group (28.6±2.8%) within all sperm also was significantly lower than other treatment groups (Tissue: 44.2±1.8% and Water: 36.2±2.0%) (p<0.05). And mitochondrial intact in Air treatment group within live (97.1±0.4%) and all (61.9±3.3%) sperm were significantly higher than other treatment groups (Tissue: 85.2±3.3%, Water: 87.8±2.9% within live sperm and Tissue: 49.28±3.7%, Water: 42.0±3.1% within all sperm) (p<0.05). Therefore, we suggest that transportation by sinking method between warm water and air was beneficial to improvement of fertility in frozen-thawed in bull semen.