Failure in servicing has detrimental effects upon customers, which can be translated into loss of resources. As such, this study employed the conservation of resources (COR) theory and developed a novel conceptualization that captures customers’ resource losses (after a failure), as well as their complaint process, by capturing dynamic loss, activation, and recovery of customers’ resources, in the case of a service failure. The use of online features and platforms for customers to voice their complaints was conceptualised as resource integration between the customer and the firm. The outcomes revealed online complaining as self-protective and selfenhancement behaviour. It is also unravelled in this study that various personal- and firm-related resource integrations in an online complaining context could result in varying emotional states and behavioural intentions among the affected customers towards the defaulting firm. These results open up an avenue for firms to devise effective intervention strategies.

Introduction An important decision that a manufacturer has to make in distributing a product to customers is the degree of forward channel integration (Aulakh & Kotabe, 1997; Coughlan et al., 2001; John & Weitz, 1988). Transaction cost economics (TCE) developed by Williamson (1975, 1985, 1986, 1999) has been one of the leading theoretical frameworks used to explain the channel integration decision (Frazier, 1999; Watson et al., 2015). TCE is generally a theory for explaining the choice of an efficient governance structure in transactions and includes asset specificity, uncertainty, and frequency as its explanatory variables. According to Williamson (1985, 1986, 1999), much of the explanatory power of TCE is driven by asset specificity. TCE-based channel integration studies argue that as asset specificity increases, firms are expected to increase the degree of channel integration. This study proposes to extend existing research in four important ways. First, existing studies have not examined individual dimensions of asset specificity. This study examines two important dimensions discussed by TCE: human asset specificity and physical asset specificity. Second, existing studies have tended to measure asset specificity in a particular way (i.e., with a particular set of questionnaire items). This study examines the robustness of the estimated asset specificity-integration relationship to alternative measures of asset specificity. Third, existing studies have focused on firms in one country such as the United States, Canada, or Germany. This study empirically examines the roles and relative importance of human and physical asset specificity in channel integration in two countries with different cultures, the United States and Japan. Fourth, existing studies have not investigated the possibility of endogeneity between asset specificity and channel integration. This study tests whether asset specificity is endogenous in explaining channel integration through an instrumental variables and two-stage least squares (IV-2SLS) approach. Literature Review In the context of distribution channels, asset specificity refers to the extent to which durable, transaction-specific investments in human and/or physical assets are needed to distribute the product in question (John & Weitz, 1988; Klein et al., 1990; Shervani et al., 2007). Examples of such investments include (1) the time and effort employed to acquire the firm-specific, product-specific, and customer-specific knowledge needed for distribution activities, and (2) specialized physical equipment and facilities (e.g., warehouses, deliver vehicles, refrigeration equipment, demonstration facilities, and repair and service centers) (Anderson, 1985; Bello & Lohtia, 1995; Brettel et al., 2011a, 2011b; John & Weitz, 1988; Shervani et al., 2007; Williamson, 1985, 1986). According to TCE, when the assets needed to distribute a product are non-specific, the use of independent channels is a priori more efficient than the use of integrated channels based on the benefits of distribution specialists and competition in the market place (Anderson, 1985). Conversely, a high level of specific assets, whether human or physical, has important implications for the degree of channel integration. The primary consequence is to reduce a large number of relationships between a manufacturer and independent channel members to a small number of relationships, which may expose the transaction in question to opportunistic behavior. Because the unique productive value created by a high level of specific assets makes it costly to switch to a new relationship, the use of independent channels will not be effective as a safeguard against opportunism (John & Weitz, 1988; Shervani et al., 2007). Channel integration provides a safeguard against opportunism by permitting (1) the better monitoring and surveillance of integrated channels relative to independent channels, and (2) the reduction of profits from opportunistic behavior since employees in integrated channels do not ordinarily have claims to profit streams (John & Weitz, 1988). As a result, as asset specificity increases, manufacturers are expected to increase the degree of channel integration to exercise greater control over the channels (John & Weitz, 1988; Shervani et al., 2007). This leads to the following basic TCE hypothesis concerning asset specificity and channel integration: TCE hypothesis. Asset specificity will be positively related to the degree of channel integration. Existing studies of channel integration tend to provide support or partial support for the hypothesized positive relationship between asset specificity and channel integration. One limitation of key studies is that they have not fully explored the dimensions of asset specificity because they treat asset specificity as unidimensional or examine only one dimension of asset specificity. Specifically, Anderson and Schmittlein (1984), Anderson (1985), Anderson and Coughlan (1987), and Krafft et al. (2004) focus on human asset specificity. While John and Weitz (1988), Shervani et al. (2007), and Brettel et al. (2011a) consider both human and physical asset specificity in their theoretical discussions, their empirical analyses focus only on human asset specificity. Klein et al. (1990), Aulakh and Kotabe (1997), and Brettel et al. (2011a) use a single measure of asset specificity that contains distinct items measuring human and physical asset specificity. Importantly, none of these studies has examined the dimension of physical asset specificity while controlling for the impact of human asset specificity. These observations suggest that further research is needed that explicitly measures and evaluates the relative importance of human and physical asset specificity in the channel integration decision. Research Hypotheses Based on the above literature review, we seek to extend existing research by distinguishing between two types of asset specificity, human and physical asset specificity. As already explained, TCE and TCE-based channel integration studies argue that both human and physical asset specificity are positive drivers of the degree of channel integration. Thus, our research hypotheses are the following: Hypothesis 1. Human asset specificity will be positively related to the degree of channel integration. Hypothesis 2. Physical asset specificity will be positively related to the degree of channel integration. Research Methodology As shown in Table 1, previous empirical studies attempt to test the basic TCE hypothesis concerning asset specificity and channel integration using (1) a particular measure of asset specificity, (2) data from a single national survey of firms in the United States, Canada, or Germany, and (3) methods such as an ordinary least squares (OLS) regression analysis and a partial least squares structural equation modelling (PLS-SEM) approach. In contrast with these studies, we seek to test the above two hypotheses concerning two types of asset specificity and channel integration using (1) different measures of asset specificity, (2) data from parallel national surveys of firms in two countries with different cultures, the United States and Japan, and (3) the methods used in prior empirical analyses and an IV-2SLS approach, which is a widely accepted method for investigating the potential endogeneity problem of focal explanatory variables (Antonakis et al., 2010, 2014; Zaefarian et al., 2017). This research strategy is partly based on the guidelines for high-quality replication studies articulated by Bettis et al. (2016b). The aims are to assess the generalizability of important prior results using different survey data drawn from different research contexts and to assess the robustness of these results using different measures and methods, thereby providing important additional evidence that contributes to the establishment of repeatable cumulative knowledge (Bettis et al., 2016a, 2016b). We developed the survey questionnaire in several steps. Following John and Weitz (1988), Shervani et al. (2007), and Brettel et al. (2011b), the dependent variable, channel integration, was operationalized by the percentage of sales through direct channels. We measured the focal explanatory variable, asset specificity, in four ways: (1) a four-item scale of human asset specificity used by Shervani et al. (2007), (2) a four-item scale of physical asset specificity based on Bello and Lohtia (1995) and Klein et al. (1990), (3) a six-item scale of human and physical asset specificity used by Klein et al. (1990), and (4) a four-item scale of human and physical asset specificity used by Brettel et al. (2011a). We also included four control variables: environmental uncertainty, behavioral uncertainty, financial performance, and channel members’ capabilities. Based on existing studies, manufacturers of electronic and telecommunication, metal, and chemical products in industrial (business-to-business) markets were selected as the setting for the empirical test. The unit of analysis was the domestic channel integration decision made at a product-market level. Respondents were sales/marketing managers (or executives) knowledgeable about channel design and strategies. In the United States, a professional marketing research company administered the data collection. In Japan, respondents were surveyed by mail. In total, we obtained 235 usable responses from US managers and 279 responses from Japanese managers. Results and Conclusions Following similar studies (John & Weitz, 1988; Shervani et al., 2007), an OLS regression analysis was used to test the hypotheses. The results, shown in Table 1, exhibit significant explanatory power for each model. As expected, (1) human asset specificity exhibits significant positive relationships with the degree of channel integration in both the United States and Japan (Models 1 & 2). These findings support Hypothesis 1. Conversely, (2) physical asset specificity does not have the expected significant positive relationships with the degree of channel integration in both the United States and Japan (Models 1 & 3). These findings do not support Hypothesis 2. Also, (3) asset specificity (Klein et al., 1990) and (4) asset specificity (Brettel et al., 2011a), two composite measures of human and physical asset specificity, exhibit the expected significant coefficients (Models 4 & 5). Additionally, we conducted a similar analysis using a structural equation modelling approach. The results mirrored those of OLS regression, thus providing further support for it. To assess the problem of potential endogeneity between asset specificity and channel integration, we employed IV-2SLS. We used (1) the level of the product’s technical content and (2) the need for coordination between production and distribution activities as instruments for human/physical asset specificity. Our instruments were individually significant predictors of asset specificity and met the exclusion restriction. However, the endogeneity test revealed no evidence of endogeneity. Thus, asset specificity was treated as exogenous in the model. In summary, our preliminary results suggest that human asset specificity, not physical asset specificity, is relevant to the channel integration decision. This finding is significant in that TCE-based channel integration studies tend to measure only one type of asset specificity. We are currently conducting additional analyses to better understand the relationship between human and physical asset specificity, for example, (1) the effects of human and physical asset specificity on different kinds of direct distribution, and (2) a multiple equation model in which human asset specificity is a function of physical asset specificity and direct distribution is a function of both human and physical asset specificity. We believe that our results will have important implications for the ways in which managers approach the channel integration decision.

The purpose is to identify the impacts of internal and external integrations in an automotive supply chain in Thailand. Data are collected from automotive firms in Thailand and are analyzed using structural equation modeling (SEM). The result indicates that both internal and external integrations are significant factors affecting market flexibility.

목적 : 본 연구는 한국형 지역사회 통합 설문지(Korean version of Community Integration Questionnaire; K-CIQ)를 개발하고, K-CIQ의 타당도 및 신뢰도를 검증하고자 하였다. 연구방법 : 대상자는 총 300명으로 뇌졸중 환자 200명과 일반인 100명이었다. 한국형 지역사회 통합 설문지의 타당도 및 신뢰도 검증을 위해 내용타당도, 판별타당도, 수렴타당도, 구성타당도의 4가지 타당도 검증과 내적일치도, 검사-재검사 신뢰도 검증을 실시하였다. 결과 : 내용타당도 결과 문항 이해도 검증에서 15문항 모두 4점대의 점수를 보였으며, 내용 타당도 지수값이 .96으로 나타났다. 판별타당도 검증 결과 뇌졸중 환자와 일반인의 K-CIQ 총점 및 각 영역별 점수의 평균에서 집단 간의 유의한 차이를 확인하였다(p<.01). 수렴타당도 검증 결과 K-CIQ와 SF-36의 상관관계가 .197로 다소 낮았지만 통계적으로 유의한 양의 상관관계를 보였다(p<.01)으며, 구성타당도 검증 결과 4개의 요인이 추출되었다. 신뢰도 연구 결과, 내적일치도 값은 .712로 높은 수준의 신뢰도를 보였다. 검사-재검사 신뢰도 결과 모든 영역에서 통계적으로 유의한 상관관계를 보였다(p<.01). 결론 : 본 연구를 통해 뇌졸중 환자의 지역사회 통합의 정도 확인 및 재활의 결과 측정을 위한 K-CIQ의 타당도와 신뢰도를 수립하였다. 이러한 결과를 토대로 K-CIQ가 국내의 다양한 재활 중재의 결과 측정 및 연구의 기초자료로 활용되고, 사회적 독립성과 지역사회 참여의 정도를 측정하기 위한 유용한 도구로 사용되기를 기대한다.

Integration of Jinshi Inscriptions in Korea (《韓國金石文集成》) is the latest and the most huge academic achievement in Korea in recent years. The authors are Im Segwon and Yi Youngtae. The compilation style is very logical. And the textual research include annotations and translations correspond with the rubbing, It’s also practical and easy to handle. However, lots of mistakes exist in the book. The article divides the mistakes into three groups: mistakes of the figures of characters (mistakes of recognition, non-recognition and lack of characters), mistakes of punctuations and technical operation mistakes. The article cites 14 supports. Finally, the basic research is able to be helpful for future studies of stone inscription.

Although the main objective of Free Trade Agreements (FTA) is market integration among member countries, there are limited studies supporting this impact. Our study explores whether FTA has enhanced market integration between South Korea and its FTA partners, focusing on South Korea’s fishery product import market. We investigate two research questions concerning FTA impacts: first, whether trade costs declined when South Korea imported fishery products from its FTA partners after the FTA; second, if the speed of the convergence of South Korea-its FTA partners’price differential of imported fishery products on trade costs result to occur more quickly after the FTA. To determine these outcomes, we utilize a Threshold Autoregressive Model covering the sample periods from January 2002 to April 2017. Our findings demonstrate the effects of FTA on market integration are different among FTA partners. FTA has enhanced the market integration between South Korea and Norway, Vietnam, and Spain, respectively, but not for others. Therefore, we find positive evidence of FTA on fishery import market integration between South Korea and Norway, Vietnam and Spain, respectively.

본 연구에서는 우리보다 40년 앞서 다문화사회로 진입한 독일에서의 사회통합 정책과 상호문화교육에 대해 살펴보았고 2000년 PISA 학업성취도 국제 비교연구 이후, 사회통합의 새로운 동력을 찾고 모든 아동들의 동등한 출발선을 보장하기 위해 이루어진 취학 전 교육기관의 개혁, 그리고 그 과정에서의 상호문화교육 강화 정책 및 독일 유아교육기관에서의 상호문화교육 사례들을 살펴보았다. 연구의 목적은 독일의 취학 전 아동들을 위한 상호문화교육 정책과 상호문화교육 사례들을 살펴봄으로써 이제 막 국가적 차원에서 지원되고 있는 우리나라 유아교육기관에서의 다문화교육에 대한 시사점들을 도출하는 것이었다. 시사점들 중에서 중요한 것들은 다음과 같다. 첫째, 상호문화교육을 실시하는 유아교육기관은 일반 아동과 이주배경 아동을 위한 공동의 교육장소로서의 역할을 넘어서서 사회통합을 위한 장으로서의 역할을 확대하고 지역사회 네트워크의 구심점이 되도록 한다. 둘째, 상호문화교육 교육과정이나 프로그램 개발 시 인위적으로 꾸며진 상황이 아니라 실제상황에서 삶에 필요한 내용을 배울 수 있도록 실제상황에서 핵심 주제를 찾고 실생활과의 연관성 하에서 의미를 찾을 수 있도록 편성한다. 셋째, 부모참여 활성화를 위하여 부모를 자원으로 인식하고 그들 자신의 상황과 자녀교육의 전문가로서 대우해 줌으로써 주체적으로 교육활동에 참여할 수 있는 여건을 만든다. 또한 다문화 부모들이 우리나라에서 동등한 권리를 가지고 살아갈 수 있도록 이들을 지역사회에 통합시키고 사회적 네트워크를 확장할 수 있도록 지원하며 부모들의 교육역량을 강화한다. 넷째, 유아기관 종사자들이 다양한 문화적 배경을 가진 아동들을 깊이 있게 이해하고 효과적인 상호문화교육을 실행할 수 있도록 단 기간의 연수가 아니라 지속적이고 내실있는 계속교육이 필요하다. 다섯째, 아동들이 불공정과 불평등 상황에 대해 자유롭게 이야기하고 자신의 생각과 느낌을 적극적으로 표현할 수 있도록 지도한다. 이 과정에서 편견에 대한 의식화와 함께 살아가는 방법을 찾을 수 있도록 반 편견교육을 실시한다.

In product development, different divisions and businesses often have heterogeneous CAD/CAE systems and methods for expressing product data, and addressing this heterogeneity creates additional costs and causes longer development periods. To ensure successful collaboration in the design process, it is therefore imperative that different CAD, CAE, and other related systems be managed in an organic and integrated manner from the initial stages of product development. Therefore, this study suggests an integrated CAD/CAE system including optimal design in a more effective and integrated manner but also to support interfacing and the collective use of design and analysis tools. To validate the proposed method, a stiffened plate example is taken as an example. It is found that the proposed method could overcome the bottleneck of CAD and CAE such as transferability of data, though CATIA and ANSYS are used at the moment.

본 연구에서는 graphene oxide (GO)를 polyacrylonitrile (PAN)에 첨가하여 전기방사법을 이용해 나노섬유 복합막 을 제조한 뒤 물리적 특성을 관찰하였다. GO의 제조는 개선된 Hummer’s 방법을 이용하였으며, 표면처리가 되지 않은 GO의 경우 0.5 wt% 이상에서 전기방사가 이루어지지 않았다. GO의 안정성 및 분산도 증가를 개선하기 위해 계면활성제를 이용하 여 GO의 표면처리를 하였다. 표면처리가 된 GO를 사용하여 나노섬유 복합막의 GO의 함량을 0.5 wt% 이상 첨가할 수 있었 다. 특히, 표면처리가 된 GO가 첨가된 나노섬유 복합막은 향상된 물리적 특성을 가지며, 이는 나노섬유 분리막 내의 GO의 분산도와 상관관계가 있는 것으로 보인다.

On December 31, 2015, the ASEAN Economic Community had officially been launched. The direct impact of this policy will be on the field of Competition Law which differs from one country to another. The Competition Law plays an important role in ensuring fair and equitable business practices within the ASEAN. The ASEAN has its Regional Guidelines on Competition Policy to assist its member countries to increase their awareness about fair and equitable business practices. This policy only serves as a guideline and has not been adopted as an enforceable rule. Therefore, the business competition in the domestic market involving the ASEAN member’s company is still being regulated by each ASEAN member country. This paper examines and analyzes the role of the competition law in addressing the intra- ASEAN members’ unfair business practices and the needs for the harmonization of the competition law within the ASEAN Countries as a transition to promulgate the ASEAN Competition Law.

본 연구에서는 Hummer`s method를 개선하여 GO를 합성하였다. 전기방사법으로 GO + PAN 나노섬유 복합체 막을 제조하였으며, 표면특성· 인장강도· Flux 및 단백질 제거에 관한 실험을 진행하였다. 또한 GO+계면활성제, rGO로 PAN 나노섬유 복합체 막을 제작하였으며, 물리적 강도 측정 및 염 제거 실험에 활용하였다. GO나 rGO를 함유한 PAN 복합체 분리막의 경우 기계적 특성뿐만 아니라 단백질 및 염 제거에 효과적인 특성을 보여주었다. 이러한 결과를 바탕으로 폐수 속의 유기물질 제거에 효과적인 분리막 연구 기초자료로 활용될 수 있을 것으로 기대된다.

PURPOSES :This study intends to estimate the tangible benefits derived from the integration of operations of an Express·Intercity Bus Terminal and a Highway Service Area.METHODS:For the study, a highway service area was chosen to function as a bus terminal, integrating its services with that of a nearby bus terminal plagued by operating losses. The exercise also helped improve mobility owing to the introduction of local buses between the terminals and utilization of the existing infrastructure such as rest areas. Thus, a terminal that was not being utilized for its intended functionality was integrated with an existing facility to improve the utilization of both and subsequently aid local development. The impact of the exercise was then measured by evaluating the variation in utilization efficiency, operating costs, travel time between two regions (Seoul-Jeomchon and Seoul-Geumsan), and the social costs before and after the integration of the two facilities.RESULTS:The impact of the integration was an increase in utilization efficiency, a decrease in operating cost, a decrease in travel time, and a decrease in social cost in both the regions. The benefits of improved utilization and cost saving can be passed on to the citizens in the form of discounts. A local power generation facility will eventually replace the bus terminal, which can revitalize the local economy.CONCLUSIONS:The integration of the highway service area with the bus terminal is expected to have a win-win effect on the passengers as well as the operators. The study also proposes a sustainable strategic plan for existing terminals and rest areas to implement a method to compete with KTX transportation.

지금은 그 어느 때보다도 급변하는 시대로 3D 프린팅, 사물 인터넷, 웨어러블 등 새로운 기술이 정신을 차릴 수 없을 만큼 빠른 속도로 쏟아져 나오고 있다. 새로운 개념에 익숙해지기도 전에 또 다른 개념을 익혀야 하는 요즘 융합, 다학제, 디자인 혁신과 같은 단어는 시대를 규정짓는 중요한 키워드임에 틀림없다. 이처럼 이종 기술간, 산업간, 학문간 융합 트렌드의 확산으로 융합 신제품 및 창의적 서비스 개발에서 디자인의 역할이 더욱 중요해지고 있다. 정부에서도 ‘ICT 중심의 융합’을 통하여 새로운 산업을 일으키고 고급 일자리를 창출함으로써 ‘창조경제를 실현’한다 는 정책을 일관성 있게 추진해오고 있다. 이러한 글로벌 트렌드 변화에 능동적으로 대처할 수 있는 ‘ICT+디자인 융합형 인재’를 양성하기 위한 체계적인 교육과정을 개발할 필요성이 대두되고 있다. 본 연구에서는 다양한 문헌연구와 세계 우수대학 및 국내 대학에 대한 벤치마킹, 산업체, 연구소, 관련기관 등에 속한 ICT분야 전문가 혹은 디자인 분야에 종사하는 디자이너를 대상으로 하는 설문조사 등을 통하여 글로벌 산업 환경의 변화와 산업체의 구체적인 수요 분석을 통하여 ICT+디자인 융합 교육과정을 제안하였다. ICT+디자인 융합 교육과정에서는 ICT+디자인 융합분야에 대한 전문적인 지식을 함양하고 연구하기 위하여 디자인적 사고와 방법론을 학습하고 ICT+디자인 통합프로젝트 수행 및 실제적인 ICT+디자인 융합 과제를 창의적으로 해결할 수 있는 교과목으로 구성하였다. 디자인과 ICT 전공자들이 어울려 ‘디자인적 사고(Design Thinking)’를 기반으로 각자의 전문성을 가지고 서로를 이해하고 소통?협력할 수 있도록 극단적 협력(Radical Collaboration)을 통한 창의적인 협업이 가능한 교과목으로 구성하였다. 또한 산업체 전문가를 멘토로 선임하여 다양한 형태의 통합프로젝트를 수행함으로써 현장 중심형 실무 프로젝트를 경험할 수 있는 교과목으 로 구성하였다. 전체적으로 단순히 연구만 하는 것이 아니라 창의융합 공작소에서 직접 만들어 볼 수 있는 환경을 구축하여 어떠한 아이디어라도 실제 구현해 볼 수 있는 교과목으로 구성하였다.

Unlike most researches that focus on single manufacturer or single buyer, this research studies the cooperation policy for two participants of supply chain such as single vendor and single buyer. Especially, this paper deals with single vendor-single buyer integrated-production inventory problem. If the buyer orders products, then the vendor will start to make products and then the products will be shipped from the vendor to the buyer many times. The buyer is supposed to order again when the buyer’s inventory level hits reorder point during the last shipment and this cycle keeps repeated. The buyer uses continuous review inventory policy and customer’s demand is assumed to be probabilistic. The contribution of this paper is to present a mixed approach and derive its cost function. The existing policy assumes that the size of shipping batch from single vendor to single buyer is increasing, called Type 1, or constant, called Type 2. In mixed approach, the size of shipping batch is increasing at the beginning part of the cycle, and then its size is constant at the ending part of the cycle. The number of shipping for Type 1 and Type 2 in a cycle in mixed approach is determined to minimize total cost. The relationship between parameters, for example, the holding cost per product, the set up cost per order, and the shortage cost per item and decision variables such as order quantity, safety factor, the number of shipments, and shipment increasing factor is figured out via sensitivity analysis. Finally, it is statistically proved that the mixed approach is superior to the existing approaches.

This paper considers one vendor-one buyer integrated-production inventory problem. If the buyer orders products, then the vendor will start to make products and then the products will be shipped from the vendor to the buyer many times. The buyer is supposed to order again when the buyer’s inventory level hits reorder point during the last shipment and this cycle keeps repeated. Buyer uses continuous review inventory policy and customer’s demand is assumed to be probabilistic. The contribution of this paper is to develop a new approach for one-vendor-one-buyer integrated production-inventory problem.

RNA Sendai virus (SeV) vector system has no risk of being integrated into the host genome. Sendai virus (SeV) vectors expressing pluripotent factors have been used to produce integration-free induced pluripotent stem cells (iPSCs) with high efficiency from various cell types in human and mouse. In this study, we generated iPSCs from pig ear fibroblast cells using the SeV vector expressing 4 human factors (POU5F1, SOX2, C-MYC, and KLF4). Colonies were emerged at Day 14 of transduction and expressed the classical pluripotency markers (POU5F1, NANOG, and SOX2) and surface marker (SSEA1). Furthermore, they showed a domed shape and could passage over 40 times under 2i (CHIR99021 and PD0325901)-LIF and MEF feeder culture condition having in vitro differentiation ability into 3 germ layers. Next, we examined the ability of six feeder free culture conditions to maintain piPSCs in a pluripotent state. piPSCs were plated on Matrigel coated dishes in different media: 1. CM: control media (LIF culture media); 2. CM-F: CM+100 ng Fetuin-A; 3. CM-N: CM+100 ng Nanog-TAT; 4. CM-2i: CM+3 uM CHIR99021+1 uM PD0325901; 5. CM-2iN: CM-2i+100 ng Nanog-TAT; 6. CM-2iN+100 ng Fetuin-A. However, piPSC could not maintain the typical self-renewal morphology on feeder free conditions regardless of culture media tested here. Further, expression of pluripotency-related genes (Oct4, Nanog and Klf4) of piPSCs cultured on feeder free conditions could not be compared with that of iPSCs cultured on MEF feeder plate. Our results suggest that integration free pluripotent stem cell from pigs could be generated by SeV vector system and maintained their pluripotency under 2i-LIF and MEF feeder culture condition, but further optimization of culture conditions may be required.

The main point of this study is to find out duplicates and differences among various regulations from different organizations. Also, it focuses on creating a reasonably unified regulation system to standardize safety & environment management. In this study, I analyzed the commonalities and the differences of two systems which are typical korean Process Safety Management System and off-site Consequence Analysis. It is confirmed that there are 25 species of overlapped material of those two systems and assessment like handling material information, facilities lists, hazardous substances and list of machine power. Process safety report focuses on onsite workers and facility protect. On the other hand, off-site Consequence Analysis focuses on design, arrangement and management of handling facility from off-site influence. I found difference two system of Enforcement purposes and way. Contradiction of Harmful information of Chemicals Control Act and occupation safety and health acts from same material. To be specific, There are no unit rule of occupation safety and health acts. so it permit inch, psi etc. But Chemicals Control Act provides that m, Mpa units. Therefore, Each regulatory duplication of items for chemicals management, standardization is writing so that you can coordinate overlapping items in the measures the need to be presented.