Ascotis selenaria, a major geometridae moth in citrus trees, annually damages the citrus leaves and fruits. The surface of young citrus fruit were usually fed by 1st larva of A. selenaria after landing or stepping onto the citrus fruits. To protect the larval damages of citrus fruits needs to predict the accurate occurrence time of the 1st larva for spraying. Because larval occurrences is dependent on the oviposition of adult female and the eggs were not found in/on citrus trees, oviposition model of A. selenaria linked with the egg development model will be helpful of protecting larval damages on citrus fruits. Adult longevity, survival and fecundity of A.selenaria was investigated at 13, 16, 20, 24, 28, 30, 32, and 35℃. The longevity decreased as the temperature increased and the female development rates (1/median longevity) were well described by a modified sigmoid model, which was used to calculate the adult physiological age. Description of the total fecundity was used by a non-linear model: The maximum fecundity of A. selenaria was estimated as 2490 eggs and peaked temperature was 19.7℃ according to the fecundity model. The cumulative age-specific oviposition rate and the age-specific survival rate was well described by 2 parameters Weibull function and a reverse logistic curve respectively. Total fecundity model, age-sepecific oviposition model, and age-specific survival model were incorporated into the oviposition model.

Bumblebees are widely used to pollinate crops in greenhouses and fields. Here, we investigated whether different wake-up treatments during a short period of 1-3 days just before indoor rearing has any effects on oviposition and colony development of CO2-treated Bombus ignitus queens and artificially hibernated B. terrestris queens The wake-up regimes were defined as 16L for 1 day (16L-1), 16L per day for 3 days (16L-3), 24L for 1 day (24L-1), or 24D for 1 day (24D-1). Among these wake-up treatments, the oviposition rate and preoviposition period of B. ignitus queens reared at 24L-1 were 16.7-25.1% higher and 1.0-3.5 days shorter than other wake-up treatments. B. terrestris queens reared at 24L-1 also showed the best results for egg-laying characteristics, which were 8.9-18.8% higher for oviposition and 0.6-3.5 days shorter for preovipostion period than other wake-up treatments. Furthermore, B. terrestris queens reared at 24L-1 were 17.5% and 13.8% higher in rate of colony foundation and queen production, respectively, than other wake-up treatments. These results show that the most favorable wake-up treatment just before rearing for egg-laying and colony developmental characteristics of B. ignitus and B. terrestris queens was 24L-1. Overall, our findings indicate that a wake-up treatment just before rearing was effective for colony initiation and colony development of bumblebee queens.

Bumblebees are widely used to pollinate crops in greenhouses and fields. Here we firstly developed an apparatus for the oviposition induction of the bumblebee Bombus ignitus using electricity. The apparatus consists of boxes for colony initiation, part of temperature control, part of heat transfer, and moving shelf. The result shows that the rates of oviposition and colony foundation in the newly developed apparatus are respectively 3.9% and 5.2% higher than in the existing apparatus using hot water. More importantly, the newly developed apparatus is 75% cheaper in costs and can more save energy than existing apparatus. These results indicate that the newly developed apparatus could serve as an effective apparatus for the oviposition induction of B. ignitus.

This study was performed to investigate the escape of pine wood nematode (PWN), Bursaphelenchus xylophilus, from two vector species (Monochamus alternatus and M. saltuarius) through oviposition and feeding behavior. First, we checked number of PWNs escaped from M. alternatus emerged from three different cases of pine logs. In case A, healthy pine trees were cut into logs and left in pine forest infected with PWN. In case B, healthy pine trees were cut into logs, left in large screen cage, and let them oviposited by M. alternatus emerged from pine trees infested with PWN. In case C, pine trees which were harboring M. alternatus were cut into logs, and PWN was inoculated artificially. The M. alternatus adults emerged from the above three cases of pine logs were checked in the next year to know how many PWN they were harboring in their bodies. The percentages of M. alternatus harboring PWN (18.3 and 15.6%, respectively) and number of nematodes per vector (5,713.1±9,248.3 and 2,034.1±4,746.8 PWNs, respectively) in case A and B logs are similar to each other. However, the percentage and the number in case C (38.3% and 20,083.1±32,188.3 PWNs) were higher than those of case A and B. Among 52 M. alternatus adults harboring PWN from all the three cases, 20 adults (38.5%) were harboring more than 5,000 PWNs per beetle. And these 20 adults were harboring 97.9% of the total PWNs in 52 adults. Second, we checked the daily escape of PWNs from M. alternatus and M. saltuarius collected at pine forest infested with PWN. The PWN escaped from their vector body for 34.9±12.4 days for M. alternatus, and for 23.9±16.2 days for M. saltuarius, reaching at peak escape during the 2nd week of emergence of the two vector species. A 44.5 and 47.2% to the total PWNs escaped from vector body within 2 weeks of vector emergence for M. alternatus and M. saltuarius, respectively. The number of PWNs escaped from each vector was 3,570.6±5,189.2 and 1,556.2±1,710.3 for M. alternatus and M. saltuarius, respectively.

Nymphal development, adult longevity, and oviposition of six different species of stink bugs were compared on seeds of a soybean cultivar, Taekwangkong, and two mung bean cultivars, Kyungseonnogdu and Jangannogdu in the insect rearing room (28±2℃, 16L:8D). Nymphs of six species of stink bugs normally developed on Taekwangkong seed. While nymphs of Riptortus clavatus, Halyomorpha halys, and Dolycoris baccarum developed on seeds of Kyungseonnogdu and Jangannogdu normally, all nymphs of Plautia stali, Piezodorus hybneri and Nezara antennata died after 4th instars on Kyungseonnogdu and after 3rd instars on Jangannogdu. Each instar period tended to be increased as nymphal stages were progressed. Nymphal period was shortest on Taekwangkong seed, followed by Kyungseonnogdu and Jangannogdu, irrelevant to stink bugs species. Emergence rate, adult longevity, and preoviposition period of 6 stink bugs were different depending on the reared seeds. Of 6 different species examined, Riptortus clavatus, Halyomorpha halys, and Dolycoris baccarum which fed on Jangannodu did not emerge, Plautia stali, Piezodorus hybneri, and Nezara antennata which fed on Jangannodu did not oviposit.

This study was conducted to determine whether trichome density affects the oviposition behavior of adult female Tetranychus urticae Koch on host plant leaves. Experiments were conducted with twenty replications on the leaf discs of each plant (Pear, 'Niitaka'; Apple, 'Fuji'; Strawberry, 'Meahyang'; 3cm diameter) at 25℃, 60-70% RH and a photoperiod of 16:8 (L:D) h. One female T. urticae was placed on each disc. The number and locations of laid eggs were recorded at 24 h intervals until T. urticae died. The trichomes were distributed along the midrib of abaxial surface of pear leaves but were evenly distributed on that of apple and strawberry leaves. Eggs were mostly laid along the midrib of pear leaf disc, but eggs were laid not only along the midrib but also randomly over the leaf disc of apple and strawberry. Therefore, it appeared that T. urticae preferred to lay eggs on the specific location where trichomes were densely distributed. Further study is needed to quantify how different distribution patterns of T. urticae eggs on different plant leaves affect the efficacy of predatory mites to control T. urticae.

Oviposition preference of the bean bug, Riptortus clavatus to sites on a plant and within a field, to plants at different developmental stages, and to different leguminous germplasms was observed. The insect layed its eggs mainly on the back surface of leaf in the upper half of plant in both observation from oviposition cage and soybean field. The egg number in fields were observed at a statistically-same rate in marginal and inside area, and at a median value of one egg per leaf in oviposited leaves. Full seed stage of soybean hardly affected oviposition preference of the insect. A statistically-significant difference in oviposition to different leguminous germplasms was observed.

In order to select the superior lines of bumblebee queens(Bombus terrestris) in oviposition and colony development which has some characteristics of diapause with adult type, undiapaused bumblebee queens of 3rd generation were surveyed in room condition compared to queen treated with CO2 and artificial hibernation. Oviposition rate and preovipositional days of undiapaused queens was lower in efficacy with 46.7% and shows same trend in preovipositional days with 52.8 days than the other treatment with CO2 anesthesia and artificial hibernation with 60.0%, 86.7%, 26.1 days and 26.6 days respectively. In continuous experiment, we also surveyed ovipositional characteristic and colony development of three types of queens emergenced from undiapaused queens and treated queens with CO2 and artificial hibernation. Although undiapaused queens in 1st generation were more effective in the same condition than treated queens, but the undiapaused queens of 3rd generation emergenced from undiapaused queens of 2nd generation were very low trend in all checkpoints compared with treated queens in same generation. This results was shown that the queens treated with CO2 or artificial hibernation need to be in successive mass rearing continuously in the year round.

망사시험관을 이용하여 20, 24, 28 및 에서 톱다리개미허리노린재의 난 및 약충발육과 성충수명 및 산란수를 조사하였다. 부화율은 에서 100%였으며, 이 온도에서 멀어질수록 부화율은 낮아졌다. 난기간은 에서 7일 이었으며, 온도가 낮아질수록 날기간이 길어져 에서 16.7일이었다. 영기별 발육기간은 1령에서 일로 가장 짧았으며, 영기가 증가할수록 발육기간이 점점 길어져 5령에서 일로 가장 길었다. 약충기간은 20, 24, 28 및 에서 각각 38, 30, 23 및 18일 이었다. 우화율은 20, 24, 28 및 32에서 16, 41, 72 및 68% 이었다. 암컷 및 수컷성충의 수명은 에서 각각 63 및 60일로 가장 길었고, 에서 각각 20 및 19일로 가장 짧았다. 산란전기간은 에서 11일로 가장 길었으며, 온도가 높아질수록 현저하게 짧아져 에서 5일로 가장 짧았다. 산란수는 에서 67개로 가장 많았고, 에서 21개로 가장 적었다. 발육임계온도는 알의 경우 였고, 1, 2, 3, 4 및 5령충은 각각 9.3, 12.7, 10.0, 11.0 및 였다.

우리나라에서 한약재로 활용되고 있는 흰점박이꽃무지(Protaetia brevitarsis)와 장수풍뎅이(Allomyrina dichotoma)의 산란과 발육에 관여하는 환경요인을 밝히고자 본 시험을 실시하였다. 부엽토수분함량은 두 종 모두에서 과습이나 과습은 산란을 저하시켰고 산란 시 최적의 수분함량은 로 추정되었다. 부엽토 수분함량은 흰점박이꽃무지와 장수풍뎅이의 알 및 유충의 생존율과 발육기간, 그리고 유충의 섭식활동에 크게 영향을 주는 것으로 이들의 생육에 관여하는 가장 중요한 요인이었다.

본 연구는 제주지역에서 계절초기 벚나무깍지벌레 방제적기를 구명하고자 월동성충의 산란시기 및 부화약충의 이동시기를 조사하였으며, 이 자료를 기존에 보고된 뽕나무깍지벌레 적산온도 예찰 모형에 적합하여 비교 검토하였다. 벚나무깍지벌레 암컷 월동성충은 4월 중하순부터 산란을 시작하여 5월 상순에는 그 알이 부화하기 시작하였으며, 5월 중순경 부화약충의 이동이 가장 활발하였다. 이와 같은 봄철 벚나무깍지벌레 발생시기는 우리나라 남부지역 및 일본 중부지역에서 보고된 뽕나무 깍지벌레 발생시기와 대체적으로 일치하였다. 또한 봄철 뽕나무깍지벌레의 >50% 부화 난괴 발생비율(y) 추정 적산온도 모형(y=1/[exp(-(-a+bx))], , a=-18.80, b=0.073, x=적산온도, 1월 1일부터 발육영점온도 적용)은 벚나무깍지벌레 부화약충 이동시기와 일치하여 봄철 벚나무깍지벌레 방제적기 추정에 유용하게 이용될 수 있을 것으로 판단되었다.

밤나무혹벌 산란동아수의 품종별 차이를 비교한 결과, 석추 및 다압에서 가장 적었고 전체적으로 신초의 하부로 갈수록 산란동아수가 많았으며 특히, 옥광, 축파, 유마 에서 이러한 현상이 뚜렷하였다. 대부분의 품종에서 동아당 평균 1.0개 이상 다중 산란되었으며 신초하부의 경우 산란수가 재래종, 축파, 옥광, 평기에서는 3.0개 이상이었으며 석추와 다압은 1.0개 미만이었다. 산란동아별 난수는 모든 품종에서 상부의 동아에 산란된 난수가 가장 적었고, 하부의 동아에 많은 경향을 보였다. 신초하부의 경우 축파는 산란된 알이 평균 15개 이상으로 가장 많았던 반면, 다압은 한개 이하로 가장 적었다. 품종별 산란비율은 모든 품종에서 밤나무혹벌이 산란한 동아를 발견 할 수 있었으며, 재래종이 로 가장 높았고, 축파와 유마에서는 각각 78.5와 이상의 산란율을 나타내었다.

잎응애류의 포식성 천적인 민깨알반날개의 실내사육에 필요한 몇 가지 생태적 특성 즉 산란 습성, 성충우화율, 저장온도 그리고 귤응애의 밀도억제효과를 시험하였다. 민깨알반날개 암컷성충은 유자잎 뒷면에 를 산란하였으며, 그중 를 귤응애의 탈피각이나 배설물로 알 표면을 피복하였다. 과수원 내 밭토양에서는 민깨알반날개의 우화율이 였는데, 밭토양 대신 원예용상토를 사용했을 때 로 향상되었다. 대부분의 유충은 지면으로부터 2 cm이내 깊이에서 번데기가 되었다. 성충 저장에 가장 적합한 온도는 발육영점온도인 이며, 저장 10, 20, 30일 후에 생존율은 각각 96.7, 73.3, 였다. 유자에 발생한 귤응애 방제를 위한 효율적인 방사비율은 150:1 미만으로 방사하였을 때 가장 효율적이었다.