밤잎의 기능성 식품 및 의약품으로서의 활용도를 높이기 위한 일환으로 밤잎의 화학성분, 항산화 및 항균활성을 측정한 결과는 다음과 같다. 일반성분은 총당 11.95%, 조지방 11.50%, 조섬유 10.11%, 조단백질 7.50% 및 회분이 1.79%순으로 나타났으며, 주요 무기성분으로는 Ca과 K으로 그 함량은 각각 215.7 mg%와 196.6 mg%로 나타났다. 비타민 C의 함량은 12.5 mg%였으며, 유리당은 glucose 3.33%, f

비파 부위별 추출물의 항균성과 항산화 활성을 조사한 결과는 다음과 같다. 과피 및 종자의 추출수율은 부탄을 분획물에서 각각 4.95%와 4.42%로 높게 나타난 반면, 잎, 씨를 제거한 과실과 과육은 물분획물에서 각각 5.10%, 23.60% 및 21.10%로 높게 나타났다. 항균활성은 잎과 씨를 제거한 과실의 경우 clear zone이 각각 Staphylococcus aureus에서 19 mm, 15mm, Escherichia coli에서 15

본 연구에서는 다양한 기능성을 가지는 저령을 80% methanol로 추출한 후 그 농축액을 ehyl acetate와 water로 순차 분획하고, 각 분획물을 이용하여 인간유래 위암세포인 SNU668의 생장 저해 효과와 항산화 효과 및 각종 위장질환의 원인균으로 보고된 H. pylori에 대한 항균활성을 조사하였다. 인간유래의 위암세포인 SNU668에 대한 저해 효과는 methanol fraction 1 mg/의 농도에서 가장 강한 암세포 저해

천연보존제인 참나무 목초액을 항균제 및 주방기구 세척시 소독제로 활용하기 위하여 식품부패균 및 식중독균에 대한 항균효과를 조사하였다. Paper disc법에 의한 시험결과 모든 시험균주에 대하여 20 첨가시 항균효과를 나타내었으며, 미생물이 첨가된 배지에 참나무 목초액을 70 첨가한 후 에서 3일간의 배양동안 모든 시험균주의 성장이 완전히 억제되었다. 증류수로 희석한 목초액 50에 대수증기까지 배양하여 멸균수로 희석한 대장균을 미량 첨가하여 에서

각종 식중독균에 대한 마린엑스의 항균활성을 측정하고, 두부에의 처리효과에 대하여 조사하였다. Paper disc법에 따른 항균활성은 100ml의 마린엑스 첨가시에도 시험균주 모두에서 강하게 나타내었으며, 마린엑스를 각 시험균주에 농도별로 첨가한 후 균의 증식을 조사한 결과 첨가농가 클수록 균의 증식을 더 크게 억제하였다. 마린엑스를 희석한 물에 두부를 첨지하여 상온에 저장하면서 pH, 색도, texture, 대장균수 및 생균수를 조사한 결과 두부

Yields of various solvent extracts for Zanthoxylum schinifolium and Z. piperitum leaves were higher in ethanol extract layer. Ethanol Extract in sancho and chopi leaves was fractionated with different solvents, such as hexane, chloroform, ethyl acetate, butanol and water, yields of hexane fraction was higher. In the solvent extracts using methanol, ethanol, hexane, chloroform, and water, ethanol extract showed the most effective antimicrobial activities. Ethanol extract in sancho and chopi leaves was fractionated, the most antimicrobial activities of sancho leaf were ethyl acetate fraction, and chopi leaf were chloroform fraction.

한국산 작두콩을 종자, 자엽 및 껍질로 구분하여 항균활성성을 조사하였다. 용매별 추출수율은 껍질이 다른 부위에 비해 물과 메탄올 추출물에서 높게 나타났으며, 메탄올 추출물의 계통분획별 추출수율은 물 분획 층에서 종자 3.3%, 자엽 2.5%, 껍질 0.3%로 높게 나타났다. 작두콩의 부위별 추출물의 항균활성은 용매별 추출물에서는 메탄올 추출물이 높게 나타났으며, 계통 분획 추출물에서는 에칠 아세테이트 분획층에서 종자, 자엽, 껍질 모두 항균활성이

백년초(손바닥 선인장)는 제주도를 비롯한 우리나라의 남해안에서 많이 생산되는 선인장의 일종으로 오래전부터 그 임상효능이 알려져 여러 가지 기능적 특성을 갖는 것으로 생각된다. 따라서 본 연구에서는 추출용매 및 방법에 따른 백년초 추출물에 대한 항균 및 항산화성에 대하여 조사하였다. 백년초의 항균활성은 대체적으로 환류추출법이 상온에서의 진탕추출법보다 조금 강하게 나타났고, 각 용매간에는 큰 차이가 없었다. 또한 모든 시험균주에 대하여 항균활성이 강

자소잎중 물과 에탄올을 이용하여 얻은 추출물을 2종의 부패곰팡이(Aspergillus flavus KCTC 6143, Aspergillus jlavus KcrC 6961) 및 5종의 식중독세균 ( isteria monocytogenes ATCC 15313, Staphylococc따 aureus 196E ATCC 13565, Escherichia coli 0157:H7 ATCC 43895, Salmonella typhimurium ATCC 1331

Ethyl acetate, methanol, water extract and their fractions from chestnut flower(Castancea crenata) were tested for antimicrobial activities. Yields of prebloomed chestnut flower extracts were 13.84, 12.90 and 1.82% in methanol, water and ethyl acetate, and those of the postbloomed were 13.12, 11.75 and 1.18%, respectively. Methanol extract from the chestnut flower was fractionated by solvents using hexane, chloroform, ethyl acetate, n-butanol and water, yields of those fractions were 0.16, 0.08, 1.94, 4.75 and 6.91% in the prebloomed, and were 0.90, 0.13, 1.40, 3.42 and 7.18% in the postbloomed. In the solvent extracts of water, ethyl acetate and methanol, methanol extract showed the most effective antimicrobial activity, antimicrobial activity of ethyl acetate fraction of methanol extract was stronger than others. Minimum inhibitory concentration of ethyl acetate fractions from the prebloomed showed 100, 140, 100 and 90ppm against Staphylococcus aureus, Streptococcus mutans, Echerichia coli and Salmonella typhimurium, in fractions from the postbloomed were 140, 140, 100 and 150ppm, respectively. Growth of all the strains was completely inhibited to 30 hours in a 150ppm concentration. E. coli sells treated with ethyl acetate fraction was collapsed severely.

Corni Fructus extracts were investigated for antimicrobial and antioxidative activities. In the methanol, ethanol, water extracts from Corni Fructus, antimicrobial activity of the water extract was stronger than the others. Antimicrobial activities were strong against microorganisms such as Streptococcus mutans, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli and Escherichia coli O-157, the activity against Escherichia coli O-157 was the strongest of all the microorganisms. The growth of all the microorganisms were completely inhibited to 48 hours in the 150 per of culture media. the shape of E. coli O-157 treated with Corni Fructus water extract was expended and its cellular surface was collapsed severely. When Corni Fructus water extract of 200 was treated, hydrogen donating effect was more than 90%, and antioxidative activity using linoleic acid was similar to 0.1% BHT at the concentration of 50.

목단피의 메탄을 추출물과 용매 분획물을 이용하여 항균작용, 프리라디칼 소거작용 및 항산화 작용을 검색하고, HPLC와 GC를 이용하여 페놀화합물을 분석하였다. 항균작용은 에칠아세테이트 분획에서 가장 높게 나타났으며, 그람 음성균보다는 그람 양성균에 대하여 더욱 효과적이었다. 에칠아세테이트 분획의 MIC를 측정한 결과, 그람 양성균에 대하여는 156-1250/ml, 그람 음성균에는 2500-5000/ml 이었다. DPPH 라디칼에 대한 프리라디칼

Korean medicinal herb extracts(KMHE) were applied to the preservation of greenhouse produce in order to prove their effectiveness. KMHE showed remarkable antimicrobial effects against Bacillus cereus, Peudomonas syringae, and Corynebacterium xerosis causing the postharvest decay of greenhouse produce. Among KMHE the extracts of Rheum palmatum L. and Coptis chinensis Franch most obviously inhibited the growth of microorganims causing the Postharvest decay of greenhouse produce, which destroyed to undetectable levels when treated with more than 500ppm of KMHE. The activities of KMHE were stable in the wide spectrum of pH and temperature. Direct visualization of microbial cells by using both transmission electron microscope and scanning electron microscope showed microbial cell membrane the function of which was destroyed by treating with the dilute solutions of KMHE. This change of cellular membrane permeability could be identified in the experiment that O-nitrophenyl--D-galactopyranoside(ONPG), the artificial substrate of -galactosidase, was hydrolyzed in the presence of KMHE, indicating that the membrane was perturbed by KMHE.

The water extract of mustard seed was fractionated by solvents with an increase of polarity, and antimicrobial activities of each extracts were examined, and volatile compounds of each extract were identified by GC-MS. When the water extract was fractionated with solvents, the antimicrobial activities were high in the order of chloroform, ethylacetate, hexane, butanol and aqueous layer. In chloroform fraction, 16 volatile compounds, including 2 isothiocyanates such as 3-isothiocyanato- 1-propene and 4-isothiocyanato- 1-butane, 1 nitrile and 4 acids were identified, their contents were higher than other fractions. Twelve, 10, 4 and 7 volatile compounds were identified in ethylaceate, hexane, butanol and water fractions, respectively. The volatile compounds were considerably less in the fractions of butanol and water than others.

To investigate the antimicrobial activities and effect of grapefruit seed extract(GFSE) on the physiological function of microorganism, antimicrobial activity, fatty acids of bacterial cell lipid and amino acids of bacterial cell protein were measured. The change of cell morphotype was observed by transmission electron microscope. GFSE was very stable on the wide range temperture and pH. The growth rate of E. coli and B. suvtilis were decreased above 40ppm GFSE There fore, minimum inhibitory concentration (MIC) of the E. coli and B. subtilis to GFSE were determined around 40ppm. In the change of fatty acids quantities, hexadecanoate was significantly decreased on the treatment compared with control in case of E. coli, whereas tridecanoate was not detected in case of B. subtilis. In the change of amino acids quantities, alanine, glutamic acid, glycine, lysine were decreased on the treatment compared with control in case of E. coli and B. subtilis Transmission electron microsgraphs(TEM) showed the microbial cells were destroyed by GFSE.