Hertwig's epithelial root sheath (HERS) consists of bilayered cells derived from the inner and outer dental epithelia and plays important roles in tooth root formation as well as in the maintenance and regeneration of periodontal tissues. With regards to the fate of HERS, and although previous reports have suggested that this entails the formation of epithelial rests of Malassez, apoptosis or an epithelialmesenchymal transformation (EMT), it is unclear what changes occur in the epithelial cells in this structure. This study examined whether HERS cells undergo EMT using a keratin-14 (K14) cre:ROSA 26 transgenic reporter mouse. The K14 transgene is expressed by many epithelial tissues, including the oral epithelium and the enamel organ. A distinct K14 expression pattern was found in the continuous HERS bi-layer and the epithelial diaphragm were visualized by detecting the β-galactosidase (lacZ) activity in 1 week postnatal mice. The 2 and 4 week old mice showed a fragmented HERS with cell aggregation along the root surface. However, some of the lacZ-positive dissociated cells along the root surface were not positive for pan-cytokeratin. These results suggest that the K14 transgene is a valuable marker of HERS. In addition, the current data suggest that some of the HERS cells may lose their epithelial properties after fragmentation and subsequently undergo EMT.

Malignant tumor cells outgrow new blood vessel formation and tend to be in hypoxic state. Hypoxic cancer cells adapt to hypoxic conditions by transforming its characteristics. On the other hand, one of the most important features of cancer cells is that carcinoma cells loses its inherent epithelial phenotype and acquires mesenchymal characteristics, called as epithelial-mesenchymal transition(EMT). It has been already well known that EMT contributes to tumor invasion and metastasis. The present study investigated whether hypoxia play a major role in induction of phenotypic changes of oral squamous cell carcinoma(OSCC). Furthermore, the mechanism of EMT in oral squamous cell carcinoma cells by hypoxia has been clarified. To mimic hypoxic condition, cobalt chloride and desferoxamine, well-known hypoxic mimetic agents, were used. This study shows that hypoxia suppresses the expression of E-cadherin(epithelial marker) and increases vimentin and N-cadherin( mesenchymal markers) in OSCC. In addition, α5 integrin protein, which is a receptor for fibronectin and an important molecule for tumor invasion, is prominently induced by hypoxia.

Jeongrimsaji as temple remains of Baekje Sabi period, its re-excavation has been carried three times in full scale. As a result, the buildings in left and right of lecture hall was largely identified in 3 forms. First, layout of small buildings in left and right of lecture hall. Second, layput of corridor in left and right of lecture hall. Third, layout of construction site in the north of corridor in left and right of lecture hall. Above second is included into remains of Gorye, the third is included into remains of Baekje - the remains were almost ruined then. The first form and third form in above is different each other in size of buildings, form of layout, and location. It is important to investigate these points: the time of constructing the buildings; what position have the use and function of the buildings in the temple? ; transition process of layout of the Buddhist temple. In this context, of temple remains in Baekje during the 5~7th century, the cases where construction sites in left and right of lecture hall and construction site in the north of corridor are identified, have been reviewed. Based on this review, the use and function of buildings, their transition process were investigated. The survey results show that small buildings in left and right of lecture hall existed in the middle and later period of the 6th century. During the later 6th century ~ the 7th century, construction site in narrow and long square shape was located in left and right of lecture hall instead of small buildings in the north of corridor. Therefore, it is identified that the aforementioned thing is earlier stage and the latter thing is later stage.

The main functions of a Buddhist temples are as a place for Buddhist services and a place for sermons and each ancient Buddhist temple was equipped with a main and separate building. After Zen Buddhism was first introduced, there was a tendency to change the terms to Buddhist sanctuary and altar, as set forth in the Zen Buddhism code of conduct called 'Cheonggyu(淸規)'. As such, it was thought that the division between Buddhist service space and preaching space were relatively firm until the Goryeo Dynasty. However in the period from the end of the Goryeo to the beginning of the Joseon Dynasty there was an increasing tendency of integration of the two buildings. It can be seen that, in cases where both buildings remained, statues of Buddha were enshrined in these buildings without any distinction. Eventually it is led to the tradition of duo-Buddhist sanctums.

Korea's stone buildings are varied in their types such as stone pagodas, stone lanterns, stone bridges, stoneworks, etc. These account for more than 30% over the entire cultural properties, but research achievements are lacking compared to wooden buildings. Accordingly, this study aims to identify the shape, role and transition of Tapgu, which had been used to set up boundary at a stone pagoda, one of the stone buildings. The 20th stone pagodas, which have relative accuracy in its forming year, have been studied around national treasure or treasure between 7th century and 9th century. There are a lot of different opinions about the role and meaning of Tapgu, and at this writer's option, Tapgu is defined as follows: First, each structure plays a different role. A structure to pass the load in the upper part to the ground can be seen as a stair or a pedestal, but a structure to form double foundations can be considered as Tapgu. Second, Tapgu can be used to divide areas with stones or stepping stones. As a result, the shape, role and transition of Tapgu is as follows: Firstly, when it comes to its shape, Tapgu includes flagstone type, belt type, double foundation type, compound type. Flagstone type had been used to set up boundary at stone pagodas by using foundation stone, belt type by keeping apart from stone pagodas, and double foundation stone by installing dual foundation stones. Secondly, Tapgu is considered to set up boundary in the case of flagstone and plate stone, and acts like a structure which can prevent surrounding area of stone pagoda from coming up while being stuck around stone pagodas. Belt type was installed only for the purpose of forming boundary. At the bottom, double foundation stone had been used to pass the load in the upper part to the ground in the same way as the foundation stone in the upper part, and the boundaries were set varying the size. Thirdly, when it comes to the transition of Tapgu, flagstone type of boundary stone had been installed in the 7th century, and belt type of boundary stone had been mainly installed in the 8th century. And double foundation stone had been installed in the 9th century. Comprehensively, flagstone type and belt type had been made around the 7th and 8th century when Tapgu was regarded important and stone pagoda started to be built. At the turn to the 9th century, the role of Tapgu had been increasingly losing in the construction of stone pagoda, and foundation stone started to appear.

Oxidation characteristics of benzene as a VOC were investigated using a fixed bed reactor system over transition metal catalysts. The transition metal catalysts were made by using transition metal nitrate reagent and various support materials such as γ-Al2O3, and TiO2. The parametric tests were conducted at the reaction temperature range of 200～500℃, benzene concentration of 2,000～3,000 ppm with space velocity of 10000 hr-1. The property analyses such as BET, SEM, TGA and the conversions of catalytic oxidation of VOC were examined. The experimental results showed that the BET surface areas of catalyst are 86.4∼167.7m2/g, the pore volumes are 0.049∼0.056cm3/g, and the average pore sizes of catalyst are 27∼44Å, which mean the meso pore. It was also found that the conversion of benzene oxidation reaction at 400∼500℃ with Cu/γ-Al2O3+TiO2 catalyst showed 90∼100%, which indicate that the transition metal catalyst with composite supports is very effective for the oxidation of benzene.

국제합작투자, 전략적 제휴, 제도적 특수성, 신뢰와 사회적 인식, 중국본 논문은 최근 많은 기업들이 국제합작투자를 통한 시장진출이 급증하고 있는 시점에서 중국과 같은 전환기에 있는 국가에 활동하고 있는 국제합작투자기업의 서로 상이한 전략과 그에 따른 성과차이를 규명하는 이론적 틀을 만들고자 하였다. 이를 위하여 제도적 이론의 관점에서 효과적인 국제합작투자 경영에 영향을 미치는 구조적 특성을 목적, 운영 그리고 성과 라는 측면에서 파악하고 이에 신뢰, 사

The HMG box containing protein (HBP) has a high mobility group domain and involved in the regulation of proliferation and differentiation of tissues. We screened HBP2 in glioblastoma using Suppression Subtractive Hybridization (SSH) and isolated human spermatogonial stem cell‐like cells (hSSC‐like cells) derived from patients of nonobstructive azoospermia (NOA). Expression of HBP2 was analyzed by RT‐PCR in undifferentiated stem cells (human Embryonic Stem Cells, hSSC‐like cells 2P) and spontaneous differentiated stem cells (hSSC‐like cells 4P). It was overexpressed in hESC and hSSC‐like cells 2P but not in hSSC‐like cells 4P. Also, the expression level of HBP2 was downregulated in colon tumor tissues compared to normal tissues. Specifically in synchronized WI‐38 cells, HBP2 was highly upregulated until the G1 phase of the cell cycle and gradually decreased during the S phase. Our results suggest that HBP2 was downregulated during the spontaneous differentiation of hSSC‐like cells. HBP2 was differently expressed in colon tissues and was related to G1‐progression in WI‐38 cells. It may play a role in the maintenance of an undifferentiated hSSC‐like cell state and transits from G1 to S in WI‐38 cells. This research was important that it identified a biomarker for an undifferentiated state of hSSC‐like cells and characterized its involvement to arrest during cell cycle in colon cancer.

온대저기압화는 중위도 기압계에 큰 영향을 주고 때로는 악기상의 원인이 되기도 한다. 그러나 온대저기압화에 대한 예보와 연구 양쪽 모두 많은 과제가 남아있다. 왜냐하면 태풍으로부터 변질된 온대저기압 자체의 모호한 형태 때문이다. 또한, 온대저기압화의 정의가 예보나 연구 양쪽 모두 만족시켜줄 만큼 정확하지도 않다 따라서 기상청은 2007 년에 "온대저기압화 현엽매뉴얼"을 개발하여 온대저기압화의 일련의 과정과 구조 변화 진단에 사용하였다. 이 매뉴얼에서는 1단계 최대풍속과 해수면온도, 2단계 위성영상, 3단계 단열선도, 그리고 최종단계인 4단계에서 지상일기도를 분석하였다. 이 현업매뉴얼은 온대저기압화가 일어날 때 구조변화의 이해와 감시에 도움이 될 것이다.

고속도로 및 국도 등의 인터체인지 유출부에 대한 설계기준과 연구 내용은 주로 변이구간, 감속차로, 곡선반경, 유출각 및 노즈부의 형상 등 자동차와 도로 기하구조간의 운동역학적인 관점의 안전성 확보만 주요 관심사였다. 따라서 본 연구에서는 차량 주행속도 특성에 초점을 맞추어 첫째 현행 설계기준의 이론적 의미를 해석하고, 둘째, 유출부 완화곡선에서 차량의 주행속도 및 교통사고 특성을 살펴보았고, 셋째, 기존의 인터체인지 유출 램프부 최소곡선반경의 부적합성을 검토한 후 운전자의 완화 곡선부 주행특성에 맞는 새로운 완화곡선 설계방법을 제시하였고 그 결과를 산출하여 기존방법과 비교 검토한 결과 유출램프 완화 곡선부 설계속도 50km/h, 40km/h의 경우기존의 원심력과 구심력이 평형을 이루는 최소곡선반경에서 관성력이 커져 교통사고의 위험이 높아진다는 것을 확인할 수 있었고 새로이 제안한 감속주행 완화곡선에서는 기존의 완화곡선보다 관성력이 작아지고 곡선은 커져 기존보다 안전한 곡선으로 확인되었다.

Throughout history, tableware has attained an important position in human culture, and historical eras are clearly reflected in the shape of tableware items, which are tied to the cultural background of foods. In particular, the distinctive qualities of foods within a food-culture, such as the means for cooking, storing, or eating, have impacted the usage and shape of tableware along with the food behaviors of individuals. Korea, China, and Japan have all played important roles in producing limitless amounts of high quality porcelain products and take pride in being leaders of the world's porcelain production industry. Based on their natural geographical proximity and political and cultural exchanges, these three countries have long influenced one another not only in terms of technical concepts but also in improving quality within the tableware industry. Thus, by comparing Korea, China, and Japan's evolutionary interdependable variance with regard to their tableware, food-culture, and food-behavior, one can provide information on the historical stream and cultural exchanges relevant to china and porcelain. Ultimately, through the examination of tableware, the conclusions of this conceptual study offer researchers a deeper understanding of the historical stance of food-culture and contribute new and useful information for the future.

현행의 도로설계기준에서는 특정 설계속도에 따라 최소기준을 정하여 이를 토대로 설계된 도로가 해당 기준을 만족하면 주행상 안전성을 확보하는 것으로 간주하고, 이에 따라 도로설계 및 교통운영을 시행하고 있다. 그러나 실제 도로를 주행하는 차량의 속도를 조사해보면, 다수의 차량이 설계자의 의도와 다른 속도로 주행하는 것을 볼 수 있고, 이는 도로 이용자의 안전성 문제와 직결된다고 할 수 있다. 특히, 직선부에서 곡선부, 혹은 곡선부에서 직선부로 주행하는 곡선부 변이구간은 이러한 속도의 변동이 가장 심한 구간 중의 하나로 이에 대한 주행 안전성 검토가 절실하다. 따라서 본 연구에서는 첫째, 곡선부 도로 변이구간에서의 주행속도와 주행반경을 고려한 지점별 횡방향 가속도와 횡방향 가속도 변화율을 산정하고, 둘째, 이를 고려한 곡선부 도로 변이구간의 설계안전성을 평가하여 편경사, 곡선반경 등의 선형 설계요소를 검토하였다. 검토결과 현재 고려되어지고 있는 구심가속도는 실제 운전자가 부담해야 하는 구심가속도에 훨씬 못 미치고 있는 실정이며, 설계속도에 따라 구심가속도가 25.56~77.78% 증가되어 주행안정성에 문제가 발생함을 확인할 수 있었다. 본 연구는 직선-단곡선으로 이어지는 곡선부 변이구간을 대상으로 하였다.

이 연구는 태풍의 온대성 저기압화에 대해 간단히 소개하고 Evans and Hart(2003)와 Hart(2003의 객관적 온대성 저기압화 판별식을 이용하여 최근 온대성 저기압화를 거친 세 태풍(Shanshan, Yaki, Soulik)에 대한 사례분석이 이루어졌다. 500-hPa 고도장분석에서 온대성 저기압화 시작시 세 태풍 모두 중위도 경압지역으로 북상하는 공통된 특성을 보였다. 그러나 연직단면 분석에서는 온대성 저기압화의 시작전 시 태풍 중심부근의 모든 층에서 온난 다습한 특성을 보였다. 온대성 저기압화 이후에는 이 개념모델의 전형적 특성인 태풍의 서쪽영역에 한랭 건조한 특성을 나타내었다. 따라서 Evans and Hart(2003)와 Hart(2003)의 객관적 온대성저기압화의 판별식은 태풍의 온대성저기압화 시작 및 구조변화를 잘 반영하므로 기상청 예보현업에서도 유용하게 사용될 수 있을 것으로 판단된다.

The purpose of this study was to examine contemporary literature and documents on the establishment and transition of school grounds in and after the Kabo Reform(1894) that initiated early modern education. It's basically meant to shed light on the past a

Studies and reports on buddhist temples in Goryeo dynasty are rarely in the present situation. At this point of time new excavational reports and historic records offer many materials to understand a architecture in Goryeo period. Especially, temple site have various transitions, it is of great value to study. This article attempted a restorative study of a location, function, spacial arrangement, structure, building age is based on a measure and transition of temple layout that is based on the excavational result and related historic documents about Godal temple site in Yeoju. Godal temple site was a representative Zen buddhist temple in Goryeo Dynasty. It was difficult of access by watercourse because it was located in an inland in the upper Han River. Archaeological research and historical records has reveals that the temple was constructed with a minium of four levels[six terms]. We could recognized the central site of temple and buildings were constantly established, enovated or collapsed. It has went through many transition according as it encountered new sects and doctrines. Also it should perform various functions and overcome given conditions. Ultimately it let see greater diversity in the spacial composition, and layout. That is to say it were planed several separate areas, each was consisted of main buildings, once again was integrated into one temple.