Gut contents analysis is essential to predict the impact of organisms on food source changes due to variations of the habitat environment. Previous studies of gut content analysis have been conducted using traditional methods, such as visual observation. However, these studies are limited in analyzing food sources because of the digestive process in gut organ. DNA metabarcoding analysis is a useful method to analyze food sources by supplementing these limitations. We sampled marine fish of Pennahia argentata, Larimichthys polyactis, Crangon affinis, Loligo beka and Sepia officinalis from Gwangyang Bay and Yeosu fisheries market for analyzing gut contents by applying DNA metabarcoding analysis. 18S rRNA v9 primer was used for analyzing food source by DNA metabarcoding. Network and two-way clustering analyses characterized the relationship between organisms and food sources. As a result of comparing metabarcoding of gut contents for P. argentata between sampled from Gwangyang Bay and the fisheries market, fish and Copepoda were analyzed as common food sources. In addition, Decapoda and Copepoda were analyzed as common food sources for L. polyactis and C. affinis, respectively. Copepoda was analyzed as the primary food source for L. beka and S. officinalis. These study results demonstrated that gut contents analysis using DNA metabarcoding reflects diverse and detailed information of biological food sources in the aquatic environment. In addition, it will be possible to provide biological information in the gut to identify key food sources by applying it to the research on the food web in the ecosystem.

연안오염총량관리제는 지자체에 오염물질부하량을 할당하고, 오염부하 삭감뿐만 아니라 바람직한 지역발전에 있어서 아주 중 요하다. 본 연구에서는 광양만특별관리해역의 총량관리 도입에 앞서 해역으로 유입하는 오염물질의 유입부하와 광양만 해역의 수질상태 를 조사하였고, 현재 연안오염총량관리를 실시하고 있는 다른 해역의 장기적인 현황을 살펴보았으며, 수회에 걸쳐 광양만 특별관리해역 환경자문위원회의 의견을 수렴하였다. 그 결과, 광양만에도 총량관리의 필요성이 인정되었으나, 시행에 앞서 우선 COD, TN, TP, 중금속, PAHs의 지속적인 모니터링과 함께 총량관리 도입의 경제성과 효율성과 같은 타당성 검토가 이루어져야 할 것으로 판단되었다.

An estuary is a water ecosystem with a high abundance of the species diversity, due to a variety of complex physicochemical factors of the area where freshwater and ocean mixed. The identification of Corbicula species in the estuary environments is difficult because of various morphological characteristics. In this study, we provide taxonomic information on Corbicula species with taxonomic difficulties using morphological and genetic analysis. This study was conducted on clams from the Seomjin River-Gwangyang Bay, one of the major production area of marsh clam in Korea. As a result, we characterized Cytocrome C Oxidase subunit I (COI) sequences of the Corbicula. The 636 bp nucleotide sequences of COI have 98% homology among Corbicula species collected from 2 sites of Seomjin River-Gwangyang Bay. The phylogenetic analysis with 17 species of Corbicula indicated that most of the species collected from Seomjin River-Gwangyang Bay were brackish water clam (Corbicula japonica), and only one Asian clam (Corbicula fluminea). The evolutionary distance between C. japonica and C. fluminea was less than 0.003. Therefore, it was confirmed that C. japonica is phylogenetically closely related to C. fluminea. In 9 species of Cyrenidae, phylogenetic tree was classified into three lineages. These results will be used as an important data for an identification of clam species by providing genetic information for Corbicula species with a morphological diversity. Key words: Corbicula japonica, cytochrome c oxidase subunit I (

The fish community in the Seomjin River-Seomjin River Estuary-Gwangyang Bay coast continuum was investigated three times from March 2019 to October 2019. The collected species at the eight sites during the survey period were 49 species belonging to 31 families, including two endangered species. According to Bray-Curtis similarities, observations were divided into four groups based on the fish community composition; two groups (group 1, 2) and two uncategorized groups (group 3, 4). ANOSIM based on spatial and temporal groupings indicated that the spatial differences in fish communities (R=0.398, P=0.001) were relatively more important than the temporal differences (analysis of similarities, R=0.273, P=0.002). In particular, there were significant differences between groups 1 and 2 (analysis of similarities, R=0.556, P=0.001), and similarity percentage analysis revealed that Argyrosomus argentatus (9.4%), Favonigobius gymnauchen (6.9%) and Konosirus punctatus (5.9%) contributed to these differences of fish assemblages for each group. The fish fauna distributed in the Seomjin River-Gwangyang Bay ecosystem were spatially divided and the number of species and number of individuals showed seasonal differences. This study could be a basis for understanding changes in the fish community and implementing conservation and management strategies on major species within a continuous environment of the river-estuary-ocean continuum.

연구대상 해역은 노량수로를 중심으로 서측에 광양만 권역(여수해협 포함), 동측에 진주만 권역(강진만, 사천만 포함)이 위치하 고, 연구대상 해역에 위치한 여러 하천에서 유출되는 유출수가 이들 만으로 유입되고 있으며, 특히 홍수시에는 광양만 권역은 섬진강의 하천유출수가, 진주만 권역은 가화천(남강댐 방류)의 하천유출수가 대량으로 유입되고 있다. 광양만 권역과 진주만 권역은 노량수로라는 협수로로 연결되어 있으며, 섬진강과 가화천의 하천유출수 또한 노량수로를 통해 서로 영향을 주고 있다. 연구대상 해역에 위치한 섬진강 과 가화천을 포함한 51개 하천의 하천유출수로 인한 평수시와 50년빈도 홍수시의 해수교환율, 체류시간 특성을 입자추적 실험을 통해 파 악하고자 하였다. 또한 홍수시 섬진강과 가화천의 하천유출수가 미치는 영향을 파악하기 위한 실험을 추가로 수행하였다. 수치실험 결과, 평수시와 홍수시 모두 광양만 권역에 투하한 입자는 노량수로를 통해 진주만 권역으로 이동하는 것으로 나타났고, 노량수로를 통해 진주 만 권역에서 광양만 권역으로 이동하는 입자는 상대적으로 작은 것으로 나타났다. 30일 후 각 실험안별 해수교환율은 광양만 권역은 44.40~67.21%로 나타났고, 진주만 권역은 각각 50.37~73.10%로 나타났고, 각 실험안별 평균 체류시간은 광양만 권역은 7.07~15.36일로 나타 났고, 진주만 권역은 6.45~12.75일로 나타났다. 그리고 홍수시에 해수교환을은 증가하고, 체류시간은 감소하는 것으로 나타났다. 광양만과 진주만 권역에서의 해수순환 구조를 살펴보기 위해 두 폐쇄성 해역에 대해서 7개 내부 영역과 5개 외부 영역에 대한 30일 동안의 단면유 량 flux를 산정하였다. 그 결과, 평수시와 홍수시 모두 전반적으로 광양만 권역에서 진주만 권역으로 유량 flux가 이동하는 것으로 나타났 다. 홍수시 유량을 적용할 경우 연구대상 해역의 유량 flux의 주 흐름 경로는 섬진강 하천유출수가 여수해협을 거쳐 외해로 이동하는 흐름 과 가화천 하천유출수가 사천만, 진주만, 대방수로를 거쳐 외해로 이동하는 흐름으로 나타났다.

본 연구는 남해에 위치한 광양만 해역의 해양수질환경 및 해저퇴적물 환경을 파악하여 기초자료로 제공하고자 한다. 광양만 인근 해역의 12개 정점을 선정하여 2020년 5월 춘계, 2020년 8월 하계에 걸쳐 조사를 실시하였다. 표층 해수는 고무 용질의 소형바스켓, 저층은 Van Dorn 채수기를 이용하여 채수하였고, 퇴적물은 Van Veen grab sampler로 채취하였다. 분석항목은 수온, 염분, DO, COD, 영양염 류 등 13개의 항목이며, ‘해양환경공정시험기준(해양수산부, 2019)’에 준하여 분석을 실시하였다. 수온은 평균 16.11 ~ 25.66 ℃, 염분은 평 균 5.24 ~ 32.23 psu, 수소이온농도는 평균 7.87 ~ 8.19, 투명도는 0.83 ~ 1.76 m, 부유물질 농도는 평균 8.12 ~ 18.74 mg/L, COD의 경우에는 평균 0.74 ~ 1.81 mg/L, Chl-a는 평균 0.26 ~ 1.61 μg/L, 용존 무기질소는 평균 2.57 ~ 22.31 uM, 인산은 평균 0.18 ~ 0.71 uM, 규산 규소는 평 균 26.35 ~ 122.35 uM, 총 질소는 평균 0.121 ~ 1.008 mg/L, 총 인의 경우, 평균 0.025 ~ 0.082 mg/L 의 범위를 나타내었다. 퇴적물 COD의 경우 일본수산용수기준치인 20mg/g-dry(일본수산자원보호협회편, 2000)을 적용한 결과 춘계에는 넘지 않았으나 하계에는 다소 넘는 것을 나타내었다. 또한 주성분 분석을 실시하여 해역의 특성을 파악하였다.

Synthesizing one-dimensional nanostructures of oxide semiconductors is a promising approach to fabricate highefficiency photoelectrodes for hydrogen production from photoelectrochemical (PEC) water splitting. In this work, vertically aligned zinc oxide (ZnO) nanorod arrays are successfully synthesized on fluorine-doped-tin-oxide (FTO) coated glass substrate via seed-mediated hydrothermal synthesis method with the use of a ZnO nanoparticle seed layer, which is formed by thermally oxidizing a sputtered Zn metal thin film. The structural, optical and PEC properties of the ZnO nanorod arrays synthesized at varying levels of Zn sputtering power are examined to reveal that the optimum ZnO nanorod array can be obtained at a sputtering power of 20W. The photocurrent density and the optimal photocurrent conversion efficiency obtained for the optimum ZnO nanorod array photoanode are 0.13 mA/cm2 and 0.49 %, respectively, at a potential of 0.85 V vs. RHE. These results provide a promising avenue to fabricating earth-abundant ZnO-based photoanodes for PEC water oxidation using facile hydrothermal synthesis.

지난 22년 동안의 선박 통항자료와 2015년부터 2017년까지 3년 동안 매년 72시간씩 실시간 선박 통항량 조사를 통해 여수광양항의 해상교통량의 장기변동과 출입항로에 대한 통항특성을 분석하였다. 2017년도 기준으로, 여수광양항의 선박 통항척수는 약 66,000척이며, 선복 량은 약 804,564천톤으로 1996년도 189,906천톤에 비해 400 % 이상 증가하였고 위험화물 물동량은 140,000천톤으로 1996년에 비해 250 % 이상 증가한 것으로 나타났다. 실시간 선박 통항량 조사결과, 1일 평균 통항 선박은 357척이며 통항로 이용율은 낙포해역이 28.1 %, 특정해역이 43.8 %, 연안통항로와 돌산연안 및 금오도 수역이 6.8 %로 동일하였다. 다수의 항로가 만나는 낙포해역은 선박간의 병항 및 교차항행이 가장 빈번했으며, 특정해역도 주변의 연안통항로에서 소형 작업선들이 다수 진출입하여 대형 선박과 교차되는 경우가 자주 발생하였다. 화물선박 의 묘박지 투묘 대기율은 약 24 % 정도였으며, 케미컬선, 유조선 등의 위험화물 선박의 야간 통항율은 약 20 %에 달하였다. 여수광양항의 선 박 통항량은 매년 증가하지만 선박 통항로는 과거와 큰 차이가 없기에 사고의 위험이 상존한다고 볼 수 있다. 따라서 다수의 항로가 중첩되어 통항 선박간의 사고 위험이 높은 제1항로 ~ 제4항로의 준설 및 항로 확장, 항로 부근 암초 제거, 항로표지 보강 등 항로 여건을 우선적으로 개선할 필요가 있다. 또한 위험성이 높은 항만의 진출입 시간과 위험화물 선박의 통항시간을 일부 제한할 수 있도록 항행규칙을 개정할 필요가 있으며, 연안통항로를 이용하는 소형 선박들의 통항관리를 적극적으로 시행할 수 있도록 VTS체계의 고도화가 요구된다.

This study aims to evaluate the concentration of biomarkers for heavy metals and volatile organic compounds (VOCs) for the residents living in the Gwangyang industrial complex to compare with residents in the residential area as a control. A total of 810 healthy adults participated in this study, and their urinary and blood samples were analyzed for metals, including As, Pb, Cd, or Hg, and VOC compounds. All study participants also completed questionnaire surveys to collect more detailed information on personal lifestyles, dietary and drinking habits, residential housings types, and their health conditions. The geometric means of urinary levels of Cd were 1.06 g/g creatinine for those living in the vicinity of Gwangyang industrial complex and 1.41 g/g creatinine for those in residential areas (p<0.05). Furthermore, urinary mean levels of Hg were 1.39 μg/g creatinine in the industrial area and 1.23 μg/g creatinine in the control area, respectively. The concentrations of individual VOCs in blood were significantly different between the two population groups. Therefore, urinary levels of Cd and Hg were significantly higher in the local residents compared to the Gwangyang industrial areas. A further study is needed to identify adverse health effects due to environmental exposures to heavy metals, VOCs, and other pollutants in the Gwangyang industrial complex areas in the future.

Species composition and yearly variation of fish eggs and larval fishes were investigated from 2014 to 2016. During the study period, the fish eggs were identified as belonging to 14 taxa. The dominant species of fish eggs were Leiognathus nuchalis, Engraulis japonicus, and Konosirus punctatus. These three species accounted for 97.4% of the total number of individuals collected. The collected larval fishes were identified into 37 taxa, 22 families, and seven orders. The dominant species of larval fish were Leiognathus nuchalis, and Gobiidae fishes. These three species accounted for 85.4% of the total number of individuals collected. The diversity index of the larval fishes was the highest in 2014 (H’ = 1.60) and the lowest in 2015 (H’ = 1.15). The evenness index was the highest in 2016 (J = 0.51) and the lowest in 2015 (J = 0.39). The dominance index was the highest in 2015 (D = 83.5%) and the lowest in 2016 (D = 70.9%). In addition, the economically important species in this area were Engraulis japonicus, Konosirus punctatus, Hippocampus coronatus, Sebastes schlegelii, Acanthopagrus schlegeli, and Cynoglossus joyneri.

We report on the fabrication and characterization of an oxide photoanode with a zinc oxide (ZnO) nanorod array embedded in cuprous oxide (Cu2O) thin film, namely a ZnO/Cu2O oxide p-n heterostructure photoanode, for enhanced efficiency of visible light driven photoelectrochemical (PEC) water splitting. A vertically oriented n-type ZnO nanorod array is first prepared on an indium-tin-oxide-coated glass substrate via a seed-mediated hydrothermal synthesis method and then a p-type Cu2O thin film is directly electrodeposited onto the vertically oriented ZnO nanorod array to form an oxide p-n heterostructure. The introduction of Cu2O layer produces a noticeable enhancement in the visible light absorption. From the observed PEC current density versus voltage (J-V) behavior under visible light illumination, the photoconversion efficiency of this ZnO/Cu2O p-n heterostructure photoanode is found to reach 0.39 %, which is seven times that of a pristine ZnO nanorod photoanode. In particular, a significant PEC performance is observed even at an applied bias of 0 V vs Hg/Hg2Cl2, which makes the device self-powered. The observed improvement in the PEC performance is attributed to some synergistic effect of the pn bilayer heterostructure on the formation of a built-in potential including the light absorption and separation processes of photoinduced charge carriers, which provides a new avenue for preparing efficient photoanodes for PEC water splitting.