본 연구는 혈중 비타민 E 농도와 대사증후군의 관련성을 살펴보고 비타민 E의 신체에 미치는 영향을 살펴보고자 한다. 분석 자료는 질병관리본부에서 시행한 국민건강영양조사 제7기 3차 (2018년도) 자료를 사용하였으며 본 연구에서는 만 19세 이상 성인은 대상으로 혈중 비타민 E의 농도를 검사한 대상 자 중 남자 944명, 여자 1,080명을 최종 분석하였다. 자료 분석은 일반적 특성에 따른 혈중 비타민 E 농도 차이는 Chi-square와 t- test로, 혈청 비타민 E 농도와 대사증후군 하부요인들 간의 관계는 Pearson 상관 관계로, 혈중 비타민 E 농도와 관련된 대사증후군 지표는 로지스틱 회귀분석을 하였다. 대사증후군의 하부 요인과 혈중 비타민 E의 회귀분석 결과 HDL-cholesterol이 정상군이 과잉군에 비해 높았으며(OR:1.042, CI:1.028-1.056), 중성지방도 정상군에 비해 유의미하게 높았다(OR:1.010, CI:1.009-1.012)(p<0.01). 따 라서 본 연구를 통하여 환자의 연령에 맞는 영양 및 건강프로그램을 개발할 것을 제안한다.

The vitamin E enhanced transgenic soybean was developed by introducing a perilla γ- tocopherol methyltransferase gene (γ-TMT) under the control of pea vicilin promoter and a selection marker, phosphinothricin acetyltransferase (PAT) gene. With regard to the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. We studied the effects of the vitamin E enhanced transgenic soybean feeding on survival of Daphnia magna which is commonly used as a model organism in ecotoxicological studies. The Daphnia magna was fed on vitamin E enhanced transgenic soybean and non-genetically modified (non-GM) soybean (Willams 82) at 0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L concentrations, respectively. The GM soybean used for the test was confirmed to have the γ-TMT/PAT gene expression by the enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) analysis. The results showed that there was no significant differences between cumulative immobilities of Daphnia magna fed on GM soybean and non-GM soybean. The 48hr-EC50 values showed no significant differences between GM soybean (2,416 mg/L) and non-GM soybean (2,408 mg/L). The results of this study suggested that there was no significant differences in toxicity for Daphnia magna between GM soybean and non-GM counterpart.

We studi ed the difTerential elTect of vitamins A, C, U. and E on normal human 이 al keratinocyte(NHOK) , HPV-16 E6E7 immor talized human oral keratinocyte(1HOK) , Oncogene transfected HPV-16 immortalized ce1ls(OTOK) , and two ol'al sq ua mous cell line(HNSCC30‘ HNSCC31) according to carcinogenesis stage. The vitamin effect was evaluated by morphology. ce ll viabi lity. a nd orgnaotypic culture Vitamin A has a greater negative effect on growth for all NHOK IHOK HNSCC. es pec ially N-Ras t rans fected IHOK, Vitamin D & E revealed no significant cell activity on NHOK lHOK, ad OTOK Vitamin C was found increased cell viability to IHOK and OTOK 1n primary oral squmaous cell ca rcino ma (HN30 ). vitam in 0 and C showing increased cell growth , but vitamin E showing no effect 1n metastatic oral squamous cell ca rcinoma(HN31), vitamin C has prol iferative effect , but vitamin 0 & E has anti-proliferative effect Vitamin A t reated normal a nd ma lignant ce1ls by organotypic cu lt ure. showed reduction of epithelial layer and in vasion to connective tissue. , especia lly in 1HOK & oncogene-transfected 1HOK, 1n conclusion. three-dimensional culture sys tem may be useful as a model to acess the efficiency of agents such as a1l trans retinoic acid can preventing progression of these premaligant lesion to maligant oral carcinoma(ch emopreventive agent) .

총지방질의 함량은 인유(성숙유), 조제 분유 및 시유 다같이 비슷한 수준치를 보였다. 조제 분유는 인유에 비하여 인 지방질, 유리 지방산, 콜레스테롤 에스터 등이 유의하게 높은 반면 트리글리세리드는 유의하게 낮은 수준이었다. 시유와 인유는 트리글리세리드 함량에서는 비슷하였지만 기타 지방질 성분에서는 차이가 있었다. 조제 분유의 인 지방질/트리글리세리드의 비, 콜레스테롤/트리글리세리드의 비, 총토코페롤 그리고 총토코페롤 / 총지방질의 비 모두 인유는 물론 시유의 그것들에 비하여 유의하게 높은 수준이었다. 총토코페롤 / 18 : 2 Ω6의 비는 조제 분유, 시유 다같이 인유에 비하여 유의하게 높았다. 인유, 조제 분유 및 사유의 비타민 E 함량은 미국 유아 식이의 기준치를 상회하였다. 조제 분유의 포화 지방산 중에서 탄소수 8개 이하의 직쇄의 우수 지방산들은 인유의 그것들에 비하여 유의하게 높았으며, 10개 이상의 탄소수를 갖고 있는 직쇄의 우수 지방산들은 인유의 그것들과 대체로 비슷한 수준들이었다. 시유에서도 역시 탄소수 10개 이하의 것들과 18 : 0 등의 직쇄의 우수 지방산들 그리고 직쇄 및 측쇄의 기수 지방산들이 높은 경향이었고 12 : 0 은 낮았다. 불포화 지방산의 경우 인유의 18 : 2Ω6은 조제 분유와는 거의 비슷한 수준인 반면 시유의 그것에 비하여 4.5 배 높은 수준이었고 탄소수 20개 이상의 장쇄 고도 불포화 지방산들은 조제 분유, 시유 다같이 낮은 수준치를 갖고 있었다.

This study was carried out to develop of environmental risk assessments and the biosafety guide for Vitamin E enhanced transgenic soybean at LMO (Living Modified Organism) isolation field. In LMO quarantine area of National Institute of Agricultural Sciences, insect species diversities and population densities on vitamin E enhanced transgenic soybean and non-GM soybeans (Willams 82 and Seoritae) were investigated. A total of 17,717 individuals of 77 species from 8 orders were collected in LMO isolation field. In three type soybeans field, total of 5,250 individuals in Vitamin E enhanced transgenic soybean, 5,510 individuals in Willams 82, and 6,957 individuals in Seoritae were collected, respectively. There was no difference between the population densities of insect pests, natural enemies and other insects on Vitamin E enhanced transgenic soybean and Willams 82, while natural enemies density on Seoritae was higher than on Vitamin E enhanced transgenic soybean, but insect pests density on Vitamin E enhanced transgenic soybean was higher. These results provided the insects diversity for risk assessment survey of Vitamin E enhanced transgenic soybean and suggested that the guideline could be useful to detect LMO crops.

Background : Despite the presence of various bioactive compounds in ginseng, there is lack of study on the variations of bioactive compounds in ginseng according to the cultivation of soil and the applied fertilizer types (or amount). Therefore, this study aims to examine the variations of 37 fatty acids (FA) and 8 vitamin E (Vit-E) vitamers in 6-year-old ginseng root cultivated in different soil types with different fertilizers regimes. Methods and Results : The profiling of 37 FAs and 8 Vit-E vitamers in 6-year-old ginseng roots was measured by gas chromatography coupled with a flame ionization detector, and then these results were statistically analyzed with chemometrics. The FA and Vit-E content in ginseng roots varied significantly with respect to soil cultivation conditions due to organic fertilizer types and amounts used. Unsaturated FA in ginseng is approximately 2.7 fold higher than the saturated FA. Linoleic, palmitic, and oleic acids were the most abundant FAs found in the ginseng roots. Also, the major Vit-E vitamer found in ginseng root is α -tocopherol. In particular, the application of rice straw compost or food waste fertilizer was increased to create nutritionally-desirable FAs and bioactive Vit-E in ginseng root. In addition, phytonutrient profiling coupled with chemometrics can be used to discriminate the cultivation conditions of ginseng. Conclusion : This preliminary study extends our understanding about the variations of FA and Vit-E in ginseng root depending on cultivation conditions. Hence, these results can be useful as basic information for reliable ginseng production containing high amounts of phytonutrients in a paddy-converted field.