Background : This study was carried out to investigate the possibility of cosmetics materials by comparing growth characteristics, photosynthetic rate and major functional components of Rosa multiflora and Perilla frutescens at different altitudes. Methods and Results : This experiment is being carried out in April 2018 in Namwon (500 m above sea level) and Iksan (15 m above sea level) in Jeollabuk-do. The growth characteristics of R. multiflora were investigated at the end of May. Flowers were collected at this time and used as samples for functional analysis. The growth characteristics of P. frutescens were investigated in the middle of August and the ground part was collected at this time and used as a sample for functional analysis. Photosynthetic rates were measured using LCpro+ (ADC, UK). The marker compound were investigated and analyzed using HPLC Alliance e2695 and 2998 PDA detector (Waters, USA). Photosynthetic rate (based on 1,600 μ mole of light intensity) was measured in mid-June as follows. The R. multiflora showed 9.8 μ mole․CO2/㎡/s in Iksan and 7.9 μmole․CO2/㎡/s in Namwon. The P. frutescens showed 15.0 μmole․CO2/㎡/s in Iksan and 8.8 μmole․CO2/㎡/s in Namwon. Overall, Photosynthetic rate was higher in Iksan. As a result of analyzing 18 kinds of marker compound, gallic acid and astragalin were found in R. multiflora, caffeic acid and rosmarinic acid were found in P. frutescens. Gallic acid and Astragalin of R. multiflora showed 5.4 ㎎/g and 28.4 ㎎/g in Iksan and 3.2 ㎎/g and 21.6 ㎎/g in Namwon, respectively. Caffeic acid and rosmarinic acid of P. frutescens were 2.7 ㎎/g and 49.7 ㎎/g in Iksan and 2.5 ㎎/g and 33.6 ㎎/g in Namwon, respectively. Conclusion : Comparing the yield of the harvesting parts by region, both R. multiflora and P. frutescens was higher in Namwon. As a result of quantitative analysis of four detected elements of gallic acid, astragalin, caffeic acid and rosmarinic acid, all four components were high in Iksan. It is considered that this is due to optical environment difference.

Background: The Rosa multiflora, a well-known plant belonging to Rosacea, is widely used in orthodox medicine in worldwide. However, its biological activity and cosmetic preservative efficacy have not yet been studied. Thus, this species is yet to be defined as a functional cosmetic material. Accordingly, an investigation of the above mentioned atrributes was performed on a 50% ethanol extract of Rosa multiflora. Methods and Results: The antioxidant activity was assessed through free radical scavenging assays with 2,2-diphenyl-1-picrylhydrazyl (DPPH). Additionally, the contents of total phenols and flavonoids were analyzed. The phenolic compounds were detected using HPLC. The antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans was assessed using the disc diffusion assay. The preservative effect (challenge test) on a formulation of soothing gel was performed for 28days. The DPPH radical scavenging ability, denoted by the SC50 (half maximal inhibitory concentration for DPPH radical scavenging) value was found to be 131.63 ㎍/㎖. The content of total polyphenol and flavonoid content were 202 ㎎/g and 86.77 ㎎/g, respectively. In additon, astragalin and gallic acid were identified in the extract. The antimicrobial activity of the extract against S. aureus and E. coli was observed to be 5 - 0.5%, and no significant activity was noted against C. albicans. The ethanol extracts (5% and 10%) met the preservation standards of the Cosmetics, Toiletry, and Fragrance Association (CTFA). Conclusions: Thus the ethanol extract of R. multiflora can be used in cosmetics as a natural preservative and antioxidant.

The roots of Rosa multiflora Thunberg have been used in traditional oriental medicine as remedies for rheumatic arthralgia and scabies. In this study, the anti-fungal, anti-oxidant, and anti-inflammatory activities of a supercritical extract of Rosa multiflora root were investigated in vitro. To investigate the anti-oxidant and anti-inflammatory effects of the supercritical extract, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, and the inhibition of nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were examined, respectively. In addition, the anti-fungal activities of the extract were assessed. The results showed a concentration-dependent, increase in ABTS radical scavenging activity. The supercritical fluid extracts of Rosa multiflora root exhibited low toxicity to RAW 264.7 cells at 100 μg/mL the highest concentration tested. Cells stimulated with LPS produced more nitric oxide than normal control cells; however, cells treated with the supercritical fluid extract decreased this production in a concentration-dependent manner. Finally, the supercritical fluid extracts showed significant anti-fungal activity. These results suggest that extracts of the roots of Rosa multiflora might be used to develop potent anti-fungal, anti-oxidant, and anti-inflammatory agents, and may be useful as ingredients for related new functional cosmetic materials.

현재 절화용 장미는 국가기관에서 주관하여 품종을 육성하고 있으나, 정원용 장미는 미진한 실정이다. 우리나라에 수입판매 되는 장미 묘는 주당 1,0000원~30,000원으로 로열티가 포함되어 있어 단가가 높으며, 대부분이 영국, 독일, 일본 등 외국에서 수입되고 있다. 우리나라에서 정원용 장미의 판매액은 80억 원에 달하고 이중 50억 원에 이르는 양이 수입되고 있으며, 수입량도 계속 늘어가는 추세이다. 또한 대형공원 조성 및 아파트 단지화, 학교, 도로변의 울타리용 등으로 크고 작은 정원들이 생겨나고 있어서 정원용 장미의 수요도 그만큼 증가하고 있다. 국내 정원장미 번식은 대부분 눈접묘를 사용하고 있다. 하지만 찔레 실생을 사용한 눈접묘 생산 시스템이 노동집약적이고 접사들의 고령화로 인하여 눈접묘 생산 시스템의 개선이 요구되었다. 본 실험에서는 눈접묘에 사용되는 찔레를 삽목 번식하여 대목으로 사용하기 위해 삽목 최적화 조건을 구명하고자 하였으며, 삽목 배지와 시기에 따른 경지삽 발근율 효율을 조사하였다. 무가시 품종인 찔레원예1호를 시료로 사용하였고, 삽수는 2년생 가지 중 목질화가 상당히 진행된 가지를 20cm정도 길이로 절단하여 1시간 정도 물에 침지한 후 저온창고(섭씨 4도)에 1주일 보관하였다. 배지는 질석과 펄라이트 1:1, 질석, 펄라이트를 사용하였고 시기는 2013년 9월부터 2014년 3월까지 수행 하였다. 본 실험 결과 질석과 펄라이트를 동비율로 혼합하여 사용한 배지가 발근율이 가장 좋았고 뿌리무게는 다른 두 종류 배지보다 낮았으며 그 외 줄기무게, 잎 수, 신초무게, 신초길이, 줄기 두께, 뿌리 건물중은 대동소이하였다. 시기적으로는 11월에 60%정도의 가장 높은 발근율을 보였으며 2월에 12%정도로 가장 낮은 발근율을 보였다. 찔레원예1호 외에도 여러 다양한 대목품종들의 실험이 요구되며 그 외에도 다양한 배지에서의 발근 효율을 알아볼 필요성이 제기되었다.

찔레꽃의 총 페놀함량은 열수 추출물에서 110.1-183.0mg·L-1으로 많았다. 총 플라보노이드 함량은 메탄올, 에탄올 및 열수 추출물에서 각각 73.2, 66.1, 65.8mg·L-1 순으로 많았다. 찔레 꽃 추출물의 전자공여능에서 IC50 값은 열수 추출물에서 39.9mg·L-1로 낮아 가장 효과적이었다. 아질산염 제거효과는 열수추출물이 83.4%, 메탄올 추출물이 80.1%, 에탄올 추출물은 78.4%을 나타냈다. 이러한 결과는 찔레꽃을 꽃차 등으로 이용시 유용한 정보가 될 것으로 사료된다.

In this study, to develop hair growth agents using natural products which has excellent ability to promote hair growth effect and fewer side effect, animal experiment and clinical trials was performed to evaluate hair growth promotion effect of herbal product containing Rosa mutiflora roots extracts as a main component (RMHP). 4-week-old male C57BL/6 mice were removed the dorsal hair using thioglycolate, and applied 15% EtOH solution as a negative control, 5% minoxidil as a positive control and RMHP to dorsal skin. In the results of macroscopy and photo-interpretation, RMHP group recorded 100% (+++++) of hair growth was proved to significantly stimulate hair growth against 80% (++++) negative control group. 37 patients were treated with RMHP and evaluated the therapeutic effect at 16 weeks. Hair density was significantly increased at 16 weeks after applying RMHP (125.0±4.9 FU/cm2) compared to before treatment (104.3±4.7 FU/cm2, p 〈 0.05), and hair thickness were also significantly increased (0.066±0.003 mm) than before treatment (0.055±0.002 mm, p 〈 0.05). The result of clinical photo-interpretation using 7-point rating scale assessment, after 16 weeks clinical symptoms were evaluated to significantly improve with 1.23±0.05 (p 〈 0.05). Therefore, the results of this study were observed that RMHP have hair loss prevention effect and hair growth promotion effect to hair loss patients.

본 연구는 찔레 에탄올 추출물의 세포 활성 및 티로시나아제활성 저해를 분석하였다. 찔레 추출물의 세포활성을 분석한 결과 찔레 잎과 뿌리 추출물의 경우에는 추출물을 처리하지 않은 대조군과 유사한 결과를 관찰하였다. 모든 농도에서 세포 활성이 나타나지 않아 티로시나아제 활성 저해 효과를 분석한 결과 찔레 잎과 뿌리추출물은 상대적으로 고농도(200 μg/ml)에서 약 30~32% 정도를 저해하는 효과를 나타냈다. 또한 B16 세포에서 티로시나아제활성 저해 효과를 분석한 결과 상대적으로 고농도(200 μg/ml)에서 양성대조군인 알부틴보다 20~25% 정도 낮은 저해 효과를 나타냈다. 멜라닌 함량 저해 효과를 분석한 결과 찔레 잎과 뿌리 추출물의 경우 티로시나아제의 저해 효과와 유사하게 농도 의존적인 저해효과를 나타내는 것을 관찰하였다. RT-PCR 방법으로 분석한 결과, 추출물 처리군들은 정상대조군과 비교하여 티로시나아제 mRNA 수준이 유의적으로 차이가 나지 않았으며, 또한 농도 의존적인 변화도 없었다.