The risk of various hazardous substances in aquatic environment comprises not only the concentration of substances in the environmental medium but also their accumulation in fish through complex food web and the health risks to humans through the fish. In Korea, the monitoring of residual toxicant in aquatic ecosystems began in 2016 following the enforcement of the Acts on registration and evaluation for the management of chemicals used in daily life (consumer chemical products), and attention has been paid to potentially hazardous substances attributed to them. Recently, studies have been carried out to investigate the distribution of these hazardous substances in the ecosystem and calculate their emission factors. These include the accumulation and transport of substances, such as detergents, dyes, fragrances, cosmetics, and disinfectants, within trophic levels. This study summarizes the results of recently published research on the inflow and distribution of hazardous substances from consumer chemical products to the aquatic environment and presents the scientific implication. Based on studies on aquatic environment monitoring techniques, this study suggests research directions for monitoring the residual concentration and distribution of harmful chemical substances in aquatic ecosystems. In particular, this study introduces the directions for research on trophic position analysis using compound specific isotope analysis and trophic magnification factors, which are needed to fulfill the contemporary requirements of selecting target fish based on the survey of major fish that inhabit domestic waters and assessment of associated health risk. In addition, this study provides suggestions for future biota monitoring and chemical research in Korea.

본 연구에서는 어장환경평가가 수행된 어류가두리 양식장의 조사 결과 중 일부를 대상으로 저서동물지수(Benthic Health Index, BHI)를 이용하여 양식장의 건강도를 평가하였으며, 평가 결과로 산출된 각 등급의 양식장 환경 특성을 파악하였다. 평가대상 어류가두리 양식장은 동해, 서해, 남해에 위치한 43개 양식장으로, BHI 1등급은 8개 양식장, 2등급은 4개 양식장, 3등급은 12개 양식장, 4등급은 19개 양식장이 포함되었다. 1등급 어류가두리양식장은 사질 퇴적상, 양식강도가 낮은 양식장이 포함되었으며, 2등급 어류가두리양식장은 해수 유통이 원활한 해역에 위치한 양식장이 속하였다. 3, 4등급이 속한 어류가두리양식장은 높은 강도로 양식 활동이 진행되고 있는 대다수의 양식장이 포함되었다. 3등급과 4등급의 양식장은 총유기탄소는 큰 차이가 없었으나, 다모류 군집분석의 결과는 4등급의 양식장에서의 유 기물 농축이 더 높은 것으로 나타났다.

설악산국립공원 내 북천의 어류군집 특성 및 멸종위기종의 서식현황을 밝히기 위해 2020년 4월부터 9월까지 조사를 실시하였다. 조사기간 동안 17개 지점에서 채집된 어류는 총 7과 22종 4,356개체였다. 우점종은 참갈겨니(Zacco koreanus, 41.8%), 아우점종은 금강모치(Rhynchocypris kumgangensis, 15.1%)였으며, 그 다음으로 돌고기(Pungtungia herzi, 10.1%), 가는돌고기(Pseudopungtungia tenuicorpa, 5.0%), 쉬리(Coreoleuciscus splendidus, 4.1%), 피라미(Z. platypus, 3.8), 배가사리(Microphysogobio longidorsalis, 3.5%), 어름치(Hemibarbus mylodon, 2.2%) 등의 순으로 우세하였다. 출현종 중 한국고유종은 14종(63.6%)이 확인되어 상대비율이 높았다. 법정보호종은 천연기념물 어름치 1종과 환경부지정 멸종위기 야생생물 II급의 묵납자루(Acheilognathus signifer), 가는돌고기, 돌상어(Gobiobotia brevibarba), 열목어 (Brachymystax lenok tsinlingensis) 4종이 출현하였는데, 열목어는 상류부에, 어름치와 묵납자루, 가는돌고기, 돌상어는 중·하류에 주로 서식하고 있었으며, 어름치와 가는돌고기, 열목어는 서식개체수가 많았다. 기후변화민감종(냉수성 어종)은 금강모치와 열목어 2종이 확인되었다. 군집분석 결과, 상류에서 하류로 갈수록 우점도는 낮아지나 다양도와 균등도, 풍부도는 높아지는 경향을 보였으며, 군집구조는 최상류와 상류, 중류, 하류로 구분되었다. 북천의 수질은 전체적으로 매우 좋음으로 평가되었고, 어류를 이용한 하천 건강성은 매우 좋음(11개 지점), 좋음(2개지점), 보통(4개 지점)으로 평가되어 양호하였다. 하지만 일부지역에서 하천공사가 진행되고 중류부에서 생활하수가 유입되고 있어, 이에 대한 어류 서식지 보존 대책이 요구되었다.

Paddy fields are important habitats for maintaining the biodiversity of agricultural ecosystems. Recently, as the need for safe food and biodiversity conservation has increased, the rice cultivation methods have undergone changes. This study was conducted to investigate the fish fauna in Environmentally Friendly Paddy fields (EFP) and Conventional Paddy fields (CP), and compare the characteristics of Misgurnus anguillicaudatus and Aphyocypris chinensis populations in EFP and CP. A total of 2,703 fish were collected and classified into six families and 13 species. Our results showed that there was a difference in the proportion of fish populations in the two paddy fields, and the number of individuals in EFP was higher than in CP. The weight values of M. anguillicaudatus were higher in EFP than in CP, and the total length, body length, and weight values of A. chinensis were higher in EFP than in CP. Conditional factor (K) values also showed similar trends in EFP and CP. Our results indicate that the farming practices in paddy fields have a significant effect on the characteristics of M. anguillicaudatus and A. chinensis populations, and the results of this study can be used as basic data for securing biodiversity in future agricultural ecosystems.

The fish community in the Seomjin River-Seomjin River Estuary-Gwangyang Bay coast continuum was investigated three times from March 2019 to October 2019. The collected species at the eight sites during the survey period were 49 species belonging to 31 families, including two endangered species. According to Bray-Curtis similarities, observations were divided into four groups based on the fish community composition; two groups (group 1, 2) and two uncategorized groups (group 3, 4). ANOSIM based on spatial and temporal groupings indicated that the spatial differences in fish communities (R=0.398, P=0.001) were relatively more important than the temporal differences (analysis of similarities, R=0.273, P=0.002). In particular, there were significant differences between groups 1 and 2 (analysis of similarities, R=0.556, P=0.001), and similarity percentage analysis revealed that Argyrosomus argentatus (9.4%), Favonigobius gymnauchen (6.9%) and Konosirus punctatus (5.9%) contributed to these differences of fish assemblages for each group. The fish fauna distributed in the Seomjin River-Gwangyang Bay ecosystem were spatially divided and the number of species and number of individuals showed seasonal differences. This study could be a basis for understanding changes in the fish community and implementing conservation and management strategies on major species within a continuous environment of the river-estuary-ocean continuum.

We investigated physicochemical properties and isotopic compositions of organic matter (δ13CTOC and δ15NTN) in the old fish farming (OFF) site after the cessation of aquaculture farming. Based on this approach, our objective is to determine the organic matter origin and their relative contributions preserved at sediments of fish farming. Temporal and spatial distribution of particulate and sinking organic matter (OFF sites: 2.0 to 3.3 mg L-1 for particulate matter concentration, 18.8 to 246.6 g m-2 day-1 for sinking organic matter rate, control sites: 2.0 to 3.5 mg L-1 for particulate matter concentration, 25.5 to 129.4 g m-2 day-1 for sinking organic matter rate) between both sites showed significant difference along seasonal precipitations. In contrast to variations of δ13CTOC and δ15NTN values at water columns, these isotopic compositions (OFF sites: -21.5‰ to - 20.4‰ for δ13CTOC, 6.0‰ to 7.6‰ for δ15NTN, control sites: - 21.6‰ to - 21.0‰ for δ13CTOC, 6.6‰ to 8.0‰ for δ15NTN) investigated at sediments have distinctive isotopic patterns (p<0.05) for seawater-derived nitrogen sources, indicating the increased input of aquaculture-derived sources (e.g., fish fecal). With respect to past fish farming activities, representative sources (e.g., fish fecal and algae) between both sites showed significant difference (p<0.05), confirming predominant contribution (55.9±4.6%) of fish fecal within OFF sites. Thus, our results may determine specific controlling factor for sustainable use of fish farming sites by estimating the discriminative contributions of organic matter between both sites.

Veronica L., the largest genus in the family Plantaginaceae, is widespread in various habitats. Due to their long-blooming flowers, Veronica species have high horticultural value as indoor potted, garden, and landscape plants. Furthermore, Veronica plants are extremely important owing to their notable diversity in habitat usage, ploidy level, and evolution. Several native taxa, which are of key interest in breeding programs and phylogenetic studies, have been identified in Korea. The genome sizes and chromosomal characteristics are basic cytogenetic features of all taxa, and their knowledge is a prerequisite when commencing genome sequencing projects. It can provide essential information for cytogenetic, taxonomic, phylogenetic, and evolutionary studies. Thus, cytogenetic analysis and genome size estimation of seven Veronica taxa native to Korea were conducted in this study. Fluorescence in situ hybridization (FISH) karyotype analysis and chromosome counting was conducted using metaphase chromosomes probed with 5S and 45S rDNA. Nuclear DNA content and genome size were determined using flow cytometry. FISH karyotype analysis revealed a common number of 5S loci and varying 45S signals that create distinctive rDNA distribution patterns in each taxon. The results indicated that the seven investigated Veronica taxa have calculated genome sizes (1C values) ranging from 517.1 to 862.0 Mbp. This study is the first to report the chromosome number and karyomorphology of seven Veronica taxa native to Korea, as well as the use of rDNA markers for identifying individual chromosomes. These findings contribute to the crucial understanding the genomic characteristics of species within the genus Veronica, serve as a basis for studying Veronica phylogeny and evolution, and provide valuable information for future breeding programs.

As a method to understand the ecological habits around the artificial reef, various reports such as fishing gear survey, diving, sound survey, underwater CCTV and camera, etc. are reported. Among them, the sound survey method is carried out by installing an acoustic system on the ship and can be investigated regardless of the marine environment such as time constraints and turbidity. Such method, however, takes a lot of manpower and time as the ship travels at a constant speed. Investigations around artificial reefs are being conducted in an artificial way, and a lot of time and labor are consumed as such. Maritime buoys have been operated for various purposes such as route signs, weather observation, marine environment monitoring and defense monitoring for navigation safety in the past, but studies on monitoring systems for ecological habits and distribution of fish using marine buoys are remarkably insufficient. Therefore, this study aims to develop a system that allows users to directly monitor fish group detector data by estimating the distribution of fish groups around artificial reefs and using wireless communication at sea. In order to confirm the suitability of the maritime buoy used in this study, it was operated to compare data using LTE-equipped buoys capable of wireless communication and a data logger-type system buoy. Data transmission of buoys capable of LTE communication was carried out in a 10-minute ON, 10-minute OFF method due to the limitation of the power supply capacity, and data of the data logger-type buoy received full data. We compared and analyzed the data received from the two fish detectors. It is expected that real-time monitoring of the wireless buoy detection device using LTE will be possible through future research.

Collagen is one of the most widely used biological materials in medical design. Collagen extracted from marine organisms can be a good biomaterial for tissue engineering applications due to its suitable properties. In this study, collagen is extracted from fish skin of Ctenopharyngodon Idella; then, the freeze drying method is used to design a porous scaffold. The scaffolds are modified with the chemical crosslinker N-(3-Dimethylaminopropyl)-N'-ethyl carbodiimide hydrochloride (EDC) to improve some of the overall properties. The extracted collagen samples are evaluated by various analyzes including cytotoxicity test, SDS-PAGE, FTIR, DSC, SEM, biodegradability and cell culture. The results of the SDS-PAGE study demonstrate well the protein patterns of the extracted collagen. The results show that cross-linking of collagen scaffold increases denaturation temperature and degradation time. The results of cytotoxicity show that the modified scaffolds have no toxicity. The cell adhesion study also shows that epithelial cells adhere well to the scaffold. Therefore, this method of chemical modification of collagen scaffold can improve the physical and biological properties. Overall, the modified collagen scaffold can be a promising candidate for tissue engineering applications.

화포천 습지의 어류 군집 특성을 밝히기 위해 2020년 5월부터 9월까지 3회 조사를 실시하였다. 조사기간 동안 8개 지점에서 채집된 어류는 7과 21종 735개체였다. 우점종은 치리(Hemiculter eigenmanni, 23.8%), 아우점종은 배스 (Micropterus salmoides, 10.3%), 그 다음으로 피라미(Zacco platypus, 9.5%), 붕어(Carassius auratus, 9.4%), 참붕어 (Pseudorasbora parva, 9.0%), 참몰개(Squalidus chankaensis tsuchigae, 6.7%), 큰납지리(Acheilognathus macropterus, 5.4%), 블루길(Lepomis macrochirus, 5.2%), 모래무지(Pseudogobio esocinus, 4.1%), 끄리(Opsariichthys uncirostris amurensis, 3.7%), 떡붕어(Carassius cuvieri, 3.3%) 등의 순으로 우세하였다. 법정보호종은 환경부지정 멸종위기 야생생물 II급의 백조어(Culter brevicauda) 1종이 확인되었고, 한국고유종은 참몰개 1종(4.8%)이 출현하였다. 외래종은 배스, 블루길, 떡붕어 3종이, 육봉형 어류는 밀어 1종이 채집되었다. 선행연구 결과와 비교하면, 유수역에 서식하는 어류의 비율은 감소하고 정수역에 서식하는 어류의 비율은 증가하는 경향을 보였는데, 특히 생태계교란 야생생물로 지정된 배스와 블루길은 점차 증가하는 경향을 보였다. 어류 군집분석 결과, 본류 지점들(St. 1, 3, 4, 5, 6, 8)은 우점도는 낮으나 다양도와 풍부도는 높았고, 지류(St. 2, 7) 등의 지점들은 우점도는 높으나 다양도와 풍부도는 낮았다. 어류를 이용한 하천 건강성은 나쁨(6개 지점), 보통(1개 지점), 매우 나쁨(1개 지점)으로 평가되었다. 수질 등급은 화학적산소요구량(COD)과 총유기물질(TOC), 부유물질(SS), 총대장균군수(TC) 등으로 인해 약간 나쁨으로 평가되었으며, 연도별 수질은 생물학적산소요구량(BOD), 화학적산소요구량, 부유물질, 클로로필-a(Chl-a) 등이 점차 증가하는 경향을 보였다. 화포천 습지의 안정적인 어류 서식 및 하천 건강성을 향상시키기 위해서는 수질개선 및 생태계교란종(배스, 블루길)의 체계적인 관리가 요구된다.

Total of 325 estuaries in Korea were surveyed to analyze the effect of presence of sluice gate on the estuary environment and fish community from 2016 to 2018. Fish community in closed and open estuaries showed differences generally, and the relative abundance (RA) of primary freshwater species in the closed and migratory species in the open estuaries were high. The result of classifying species by habitat characteristics in closed and open estuaries showed similar tendencies at the estuaries of south sea and west sea. The relative abundances of primary freshwater species in the closed estuaries at the estuaries of south sea and west sea were the highest, but estuarine and migratory species were high in both closed and open estuaries at the estuaries of east sea. Primary freshwater species showed higher abundances in the closed estuaries with reduced salinity due to blocking of seawater since they are not resistant to salt. However, primary freshwater species in open estuaries at east sea was higher than that of the closed estuaries, which is considered to be the result of reflecting the characteristics (tide, sand bar, etc.) of the east sea. Korea Estuary Fish Assessment Index (KEFAI) was showed to be higher at open estuaries than closed in all sea areas (T-test, P<0.001), the highest KEFAI was observed in closed estuaries at south sea, and open estuaries in east sea. Fish community of closed and open estuaries in each sea areas showed statistically significant differences (PERMANOVA, East, Pseudo-F=3.0198, P=0.002; South, Pseudo-F=22.00, P=0.001; West, Pseudo-F=14.067, P=0.001). Fish assemblage similarity by sea areas showed a significant differences on fish community in closed and open estuaries at east sea, south sea, and west sea (SIMPER, Group dissimilarity, 85.85%, 88.36%, and 88.05%). This study provided information on the characteristics and distribution of fish community according to the types of estuaries. The results of this study can be used as a reference for establishing appropriate management plans according to the sea areas and type in the management and restoration of estuaries for future.

Fish assemblage of total 325 of Korean peninsula estuaries were surveyed to analyze the characteristics of community structure and diversity by sea areas for three years from 2016 to 2018. The scale (stream width) of Korean estuaries were various (14~3,356 m), and 68.9% of all estuaries showed salinity of less than 2 psu. Total 149 species classified into 52 families of fish were identified, and the dominant and sub-dominant species were Tribolodon hakonensis (relative abundance, RA, 12.5%) and Mugil cephalus (RA, 9.5%), respectively. The estuary of the Korean Peninsula had different physical and chemical habitat environments depending on the sea area, and accordingly, fish community structure also showed statistically significant differences (PERMANOVA, Pseudo-F=26.69, P=0.001). In addition, the NMDS (nonmetric multidimensional scaling) results showed the patterns that indicating fish community difference by sea areas, even though low community similarity within sea area (SIMPER, 21.79~26.39%). The estuaries of east sea areas were distinguished from the others in the aspects of which, the higher importance of migratory fishes and endangered species, and that of brackish species were characterized at south sea estuaries. However, the estuaries of west sea showed higher importance of species that have a relation with freshwater (primary freshwater species, exotic species), which is the result that associating with the lower salinity of west sea estuaries because of the high ratio of closed estuaries (78.2%). The SIMPER analysis, scoring the contribution rates of species to community similarity, also showed results corresponding to the tendency of different fish community structures according to each sea area. So far, In Korea, most studies on fish communities in estuaries have been conducted in a single estuary unit, which made it difficult to understand the characteristics of estuaries at the national level, which are prerequisite for policy establishment. In present study, we are providing fish community structure characteristics of Korean estuaries in a national scale, including diversity index, habitat salinity ranges of major species, distribution of migratory species. We are expecting that our results could be utilized as baseline information for establishing management policies or further study of Korean estuaries.

In this study, trends in research methods and topics of seabird and marine fish migration were examined. Based on the framework of existing animal migration studies, future research directions were proposed in relation to the migration of seabirds and fish. In terms of research methodology, with the development of science and technology, tracking techniques using radio telemetry, acoustic telemetry, RFID (radiofrequency identification), satellite tracking, and geolocators are widely used to study seabird and fish migration. Research is also conducted indirectly through a population survey and the analysis of substances in the body. Research contents are largely classified into extrinsic factors that affect migration (such as environmental variables and interspecific competition), intrinsic factors such as hormones, anthropogenic activities including fishery and offshore wind farm, and the effect of global climate change. In future studies, physiological factors that influence or cause migration and dispersal should be identified concerning intrinsic factors. For the analysis of migration ability, it is necessary to study effects of changes in the magnetic field on the migration ability of seabirds and fish, interspecific differences in spatiotemporal migration ability, and factors that influence the migration success rate. Regarding extrinsic factors, research studies on effects of anthropogenic disturbances such as fishery and offshore wind farm and global climate change on the migration and dispersal patterns of marine animals are needed. Finally, integrated studies on the migration of seabirds and fish directly or indirectly affecting each other in various ecological aspects are required.

This study investigated the effect of bactocease, as a natural preservative, on the microbial growth and quality of fried fish paste during storage. To select the optimal concentration of bactocease, the inhibition effect of its different treatment concentrations (0.2, 0.4, and 0.8%) on total aerobic bacteria in fish paste dough during 5 days of storage was examined. The sample treated with 0.4% bactocease showed a 1.21 log reduction in microorganisms compared with that of the control. This result was higher than that with 0.2% bactocease and potassium sorbate and similar to that with 0.8% bactocease. Consequently, 0.4% bactocease was chosen as the best concentration for preparing the fried fish paste. Microbial growth on fried samples treated with preservatives was more inhibited by adding preservatives regardless of their type than that of the control after 15 days of storage. In addition, there were no significant differences in the quality between the samples treated with 0.4% bactocease and 0.2% potassium sorbate, such as volatile basic nitrogen contents, TBARS values, pH, and acidity. Therefore, these results indicate that food preservatives should be added into fried fish paste for controlling microbial growth, and bactocease can have a high potential to replace potassium sorbate in processed marine foods, including fried fish paste.

본 연구에서는 어류가두리양식장 시설의 재배치 이후 기존 양식장 아래 퇴적물의 회복상태를 규명하고자 하였으며, 이를 위하 여 양식장 아래 퇴적물과 저서다모류 군집 조사를 수행하였다. 양식장 철거 이전인 2017년 10월에 사전 조사를 수행하였으며, 양식장 철 거 이후인 2017년 11월부터 2018년 10월까지는 매달, 이후 2020년 10월까지는 2 ~ 3달 간격으로 조사하였다. 조사 정점은 철거된 양식장 위 치에 3개 정점(Farm1 ~ 3)과 양식시설물이 없는 주변 해역에 3개의 대조 정점(Con1 ~ 3)으로 선정하였다. 사전 조사에서 기존 양식장의 총유 기탄소(평균 22.67 mg․g-1 dry weight)는 대조 정점(평균 13.68 mg․g-1 dry weight)보다 높았으나, 양식장 철거 이후 점차 감소하여 약 1년 이 후에는 통계적으로 유의한 차이를 보이지 않았다(p<0.05). 저서다모류 군집은 여름철 기존 정점에서 무생물 군집이 출현하였으며, 무생물 시기 이후 오염지시종인 Capitella capitata 단일종이 극우점하는 낮은 다양도의 군집으로 천이하였다. 다음해 여름철 무생물 시기 이전까지 종다양도가 증가하고 오염지시종의 비율이 감소하여 저서다모류 군집이 회복되는 경향을 나타내었으며, 이러한 변화는 매년 반복되었다. 연구 지역은 양식장 아래의 지형학적인 특성으로 인하여 매년 무생물 군집이 출현하고 있으나, 조사가 진행될수록 무생물 발생 기간은 짧아지고, 군집이 회복되는 과정은 빠르게 진행되었다. 어류 양식장의 이전 후 기존 양식장 정점의 퇴적물은 생물학적인 회복이 여전히 진행 중이며, 추가적인 모니터링을 통해 회복의 경향을 연구할 필요가 있다.

This study was conducted on October 23, 2020 at the Cheongpyeong Reservoir located in Seorakmyeon, Gapyeong-gun, Gyeonggi-do, and analyzed the horizontal and vertical distribution patterns of fish based on data obtained using fishfinder. The total surface area of fishfinder survey conducted was 782,853 m2, and where the water depth (WD) ranges from 10 m to 12 m is widest which 31.7% of total surface area. As a result of the heat map analysis, fish density was highest at right bank under the Gapyeong-bridge, but there was no specific pattern in horizontal distribution of fish. As a result of vertical distribution of fish analysis, 86.6% of fishes are observed at below 6 m of the fish depth (FD, distance from water surface to fish). As a result of the relative height (RH, water depth-distance from bottom to fish ratio) analysis, there was a tendency that fishes are distributed in near surface area more as the WD increased. This tendency could have various reasons such as water temperature gradient along the water depth, and further studies are required for detailed explanation.

Learning Chinese through animation can be of great help to balanced language learning as you can access not only language but also culture. This study extracted and analyzed the vocabulary of the July 8th, 2016 Chinese animation [Big Fish & Begonia] as evaluated by HSK grade. Among the total number of vocabularies and expressions observed, the average proportion of the ‘basic HSK’ grade vocabulary is 53.95% and the average proportion of the ‘modified HSK’ grade vocabulary is 96.98%, which is very meaningful in learning. The composition ratio of HSK grade for each section was generally constant and the difficulty was constant. The animation [Big Fish & Begonia] contains various vocabulary for learning Chinese and is an educational content that embodies the cultural colors and characteristics of China well for children and adult learners in the early stages of learning Chinese. A very positive educational effect can be expected for the development of Chinese language skills, and the possibility of its use is also very high.