고추 유래 유전자(CaMsrB2-23)를 도입하여 내건성을 갖도록 형질전환된 가뭄저항성벼(Agb0103)에 대한 식품안전성 평가를 위해 가뭄저항성벼 종실(현미)의 일반성분, 지방산, 아미노산, 무기질, 비타민 함량 등 영양성분과 항영양소를 분석하였다. 벼는 수원과 군위 2개 지역에서 2013년에 재배하였으며, 가뭄저항성벼와 일미벼 현미는 조단백질, 조지방, 조회분, 조섬유 등 일반성분 함량에 유의적인 차이가 없었으며 지방산 조성 및 아미노산 함량이 서로 유사하였다. Agb0103과 일미벼 현미의 무기질 함량은 Na 함량에서 차이가 있었으나 Na를 제외하고 모든 무기질 함량이 유사하였으며 OECD 기준 범위 내에 있었다. 대체적으로 수원에서 재배한 벼 현미의 무기질 함량이 약간 높게 나타나 재배지역간의 환경적 차이가 무기질의 함량에 영향을 주는 것으로 나타났다. 두 지역에서 재배한 Agb0103과 일미벼 현미의 비타민 함량은 유의적인 차이가 없었으나 비타민 B 함량이 OECD 자료 범위보다 낮게 나타났다. 가뭄저항성벼(Agb0103)의 사료용 평가를 위해 지상부(유숙기 잎줄기와 볏짚)에 대한 영양성분을 분석한 결과 조단백질, 조지방, 조회분, 조섬유, ADF, NDF, Ca, P의 함량에서 Agb0103과 모품종벼 간의 차이는 없는 것으로 분석되었다. 따라서 본 연구를 통해 조사한 가뭄저항성벼(Agb0103)는 모품종 대비 종실의 주요 영양성분과 항영양소 뿐만 아니라 벼 지상부의 성분분석 비교 결과 실질적 동등성을 확인 할 수 있었다.

유전자변형 작물이 절지동물에 미칠 수 있는 잠재적인 부정적 영향은 유전자변형 작물의 주요한 환경위해성의 하나로 여겨지고 있다. 본 연구에서는 PPO (protoporphyrinogen oxidase) 저해 제초제 내성 유전자변형 벼가 절지동물에 미칠 수 있는 영향을 평가하기 위하여 절지동 물의 다양성과 군집구조를 조사하였다. 절지동물은 야외포장에서 벼의 생육기간 동안 황색점착트랩을 이용하여 채집하였다. 유전자변형 벼는 채 집된 절지동물군집의 다양도 지수에 유의한 영향을 주지 않았다. 또한 다변량분석(PerMANOVA, NMDS) 결과에서도 절지동물군집 구조는 채집시기에 따라 달랐지만 벼의 유전형(유전자변형 또는 비변형)에 의해 영향을 받지 않았다.

본 연구의 목적은 우리나라에서 개발된 병저항성 GM벼의알레르기 유발성을 평가하고자 하였다. 벼 유래의 Cholinekinase 1 유전자(OsCK1)를 과발현 하도록 형질전환한 벼를이용하여 알레르기 유발인자와 상동성이 있는지 확인한 결과,연속되는 80개 이상의 아미노산 절편에서 35% 이상의 상동성을 보이는 서열은 없었으며 8개씩의 인접 아미노산과 일치하는 서열도 확인되지 않았다. 또한 GM벼와 non-GM 벼 사이의 단백질 분포가 일치하는 것을 확인하였으며, 인공 위액에서 빠르게 분해됨을 확인하였다. 발현단백질의 당화 발생 여부를 확인한 결과, OsCK1단백질에 대한 당화는 발생하지 않는 것을 알 수 있었으며 열안정성 검사에서OsCK1 단백질을100oC에서 가열하였을 때 30분 이후부터는 약하게 분해됨을확인하였다. 본 연구의 결과를 통해 병저항성 GM벼는 알레르기 유발과는 상관관계가 없음을 확인하였다.

Biodiversity of arthropods in Cnaphalocrocis medinalis (Lepidoptera: Crambidae) resistant GM rice (CryIAc1) and non-GM rice fields. Sampling was conducted 15 times using sweeping net and electric aspirator. Biodiversity was analyzed with species richness and Shannon diversity index (H’). Total 28,275 arthropod individuals (12,413 in GM rice and 15,862 individuals in non-GM rice) were collected and there were 22 families, 34 genera and 36 species belonging to 8 orders. There were 19 families, 29 genera, 31 species in GM rice and 20 families 32 genera 34 species in non-GM rice fields. There was no significant difference in species richness and species diversity (H’) between GM and non-GM rice. Species diversity was significantly higher in non GM rice in the insect pest group only in middle and late of August .

Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by global agricultural companies. Until now, GM crops have not been cultivated commercially in Korea. Commercialization of GM crops requires a compulsory assessment of environmental risk associated with the release of GM crops. This study was conducted to evaluate the frequency of pollen mediated gene flow from Bt transgenic rice (Agb0101) to japonica non-GM rice (Nakdongbyeo), indica non-GM rice (IR36), and weedy rice (R55). A total of 729,917, 596,318 and 230,635 seeds were collected from Nakdongbyeo, IR36, and R55, respectively, which were planted around Agb0101. Selection of the hybrids was determined by repeated spraying of herbicide and Cry1Ac1 immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using specific primer. The hybrids were found in all non-GM rice and out-crossing ranged from 0.0005% at IR36 to 0.0027% at Nakdongbyeo. All of hybrids were located within 1.2 m distance from the Agb0101 rice plot. The meteorological elements including rainfall and temperature during rice flowering time were found to be important factors to determine rice out-crossing rate. Consideration should be taken for many factors like the meteorological elements of field and physiological condition of crop to set up the safety management guideline to prevention of GM crops gene flow.

Genetically modified (GM) crops have never been cultivated commercially in Korea, it is necessary for a thorough assessment of the risks associated with their environmental release. We determined the frequency of pollen mediated gene flow from disease resistant GM rice (OsCK1) to non-GM rice (Nagdongbyeo) and weedy rice (R55). A total of 449,711 or 164,604 seeds were collected from non-GM and weedy rice, respectively which were planted around OsCK1. Resistance of the hybrids was determined by repeated spraying of herbicide and DNA analysis using specific primer to confirm hybrids. Though non-GM rice and weedy rice have similar flowering time, the hybrids were found only in non-GM rice and out-crossing ranged from 0.018% at 0.3 m to 0.013% at 0.6 m. All of hybrids were located within 0.6 m distance from the GM rice plot in southerly direction. The meteorological factors including temperature and relative humidity during flowering time were found to be the most important factors for determining rice out-crossing. It should be considered many factors like the local weather condition and flowering time to set up the safety management policy to prevent pollen mediated gene flow between GM and conventional crop.

Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insert number of foreign genes, and analyzed the flanking sequences of T-DNA in rice genome. As a result, The T-DNA of Btt12R 3-1-1-1 line was inserted in exon region of rice chromosome 10 and Btt12R 6-1-1-1 line was inserted in two copies of foreign gene. Btt12R 9-1-1-1 line was analyzed at only left border flanking sequence. The T-DNA of Btt12R 13-1-1-1 line was inserted one copy of foreign gene between position 24,516,607~24,516,636 of rice chromosome 5 and 30bp known genomic sequences were deleted. The Btt12R 13-1-1-1 line confirmed to be inserted in intergenic region having not any expressed gene and no any deletion/addition of T-DNA sequence. From these results, we demonstrated that the molecular data of rice water weevil resistant Bt rice could be acceptable to conduct the biosafety and environment risk assessment for GM crop commercialization

This study investigated the nutrients and anti-nutrients of produced resveratrol GM rice (Iksan-515, Iksan-526, Dongjin) that were cultivated Iksan and Suwon regions. Among the rice samples, Iksan-515 and Iksan-526 are produced resveratrol GM rice. Reveratrol is health-beneficial compound with strong antioxidant and antitumor activities. Red wine is believed to the main source of resveratrol in the human diet. Recent studies have associated resveratrol with the cardio-protective effect observed among people with moderate resveratrol consumption. Moreover, resveratrol has been possess chemoprotective activity. In present study, we determined the substantial equivalence between GM rice and seedling sort. We investigated the nutrients and anti-nutrients of produced resveratrol GM rice and analyzed nutrients including moisture, crude fat, ash, crude protein, fatty acids, amino acids and minerals. The results of this analysis showed equivalence between GM rice and non-GM rice. We determined phenolic compounds including naringenin, vallin and investigated 4 tocopherols (α-, β-, γ-, δ-tocopherol) and 4 tocotrienols (α-, β-, γ-, δ-tocotrienol). Among the all rice cultivars, they showed substancial equivalence between resveratrol GM rices and non-GM rice.

Genetically modified (GM) plant claims to be the solution to global poverty, and potentially solving environmental change and food requirement by increased human population. In this study, we were evaluating agronomic characteristics and chemical properties of two GM drought-tolerant rice (CaMsrB2-8 and CaMsrB2-23) compared with donor cultivars (Ilmi). Statistical analysis agronomic characteristics GM and donor rice showed no significant difference between both of them. Yield and appearance of rice grain, GM rice was a similar to the donor rice. Chemical composition analysis showed that GM drought-tolerant rice has no different with donor rice. This result indicated that GM drought tolerant rice has no big significant difference agronomic character and chemical properties; it can be solve food shortages in spite of drought condition.

The significance of genetic stability and bio-safety environment has been recently recognized by many GM plants. This study was to evaluate the GM stability of transgenic rice and to identify the environment variance. The GM rice of vitamin A　-enriched rice and four check cultivars were analyzed the data on agronomic characters and principal component for 2009-2011 in large-GM crop field. Cultivation environment was conducted in the large-GM field and greenhouse to determine grain characters. In this experiment, there was no significant difference in agronomic characters between GM rice of vitamin A-enriched rice and a donor plant, Nagdong. Related to grain characters, grain appearance and physicochemical characteristics were similar to GM rice of vitamin A-enriched rice and a donor plant, Nagdong. However, grain appearance in GM rice of vitamin A-enriched rice showed to white core and white belly when GM rice of vitamin A-enriched rice was planted in greenhouse. The type and distribution of dominant weed species also were not different from GM rice of vitamin A-enriched rice and a donor plant, Nagdong. Additionally that of gene flow was not detected in dominant weed species by PCR analysis.

최근 GMO 작물의 재배, 생산이 날로 늘어나며 GMO 작물이 환경에 미칠 수 있는 많은 가능성들이 대두되고 있다. 특히 GMO 작물과 야생종과의 자연교잡에 의한 유전자 전이로, 잡초화의 문제점이 제기되며 생태계의 변화 및 파괴의 위험성이 우려되고 있다. 본 실험에서는 GM벼와 야생 및 근연종 사이의 교잡가능성 및 유전자 전이율을 조사하기 위한 유전자 이동의 분석 체계를 확립하고자 하였다. 벼의 개화시기에 GM벼와 야생 및 근연종 간의 인공교배 후 수확한 교잡 추정 종자를 발아시켜서 제초제를 처리하여 교잡종자를 선별하였다. 또한 GM 벼 및 야생 근연종벼들 간의 RAPD PCR 분석을 통해 선별한 marker를 사용하여 낙동 교잡벼와 샤레 교잡벼가 GM 벼와 교배된 식물체임을 확인하였다. PCR 분석을 수행한 결과 GM벼에서 도입된 trehalose-6-phosphate phosphatase (TPP) 유전자와 선별marker로 사용된 bar유전자가 GM벼 뿐만 아니라 샤레 교잡벼에도 존재하였으며, 결과적으로 GM벼의 bar 및 tpp 유전자가 잡초성벼인 샤레 교잡벼에 전이되었음을 검증할 수 있었다.

This study was conducted to investigate the crossability, seed dormancy and overwintering ability of rice plant in GM (glufosinate ammonium-resistant lines. Iksan 483 and Milyang 204) and non-GM (their parents) or red rice (Andongaengmi). Seed-setting rate was not significantly different between GM and non-GM rice varieties. Iksan 483 and Milyang 204 showed the similar level of seed germination rate from 30 to 50 days after heading as compared to non-GM rice varieties. After overwintering in paddy field, seed germination rate of GM and non-GM rice varieties ranged from 14.3 % to 57.6 % in dry soil condition, but there was no germination in wet-soil except red rice. The result in wet-soil condition may help to set up a strategy for reducing the risk of gene flow of transgene via dispersal of seeds of GM plants. The crossability, seed dormancy and seed overwintering of Iksan 483 and Milyang 204, herbicide resistant GM rice varieties, were not significantly different compared to non-GM rice varieties. The results might be helpful to reduce the risk of transgene dispersal from GM crop via seeds and pollens.