Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) affects bread and noodle processing quality, the function of specific LMW-GS proteins mostly remain unclear. It is important to find a corresponding gene for a specific LMW-GS protein in order to understand the function of the specific LMW-GS protein. The objective of this study was to identify LMW-GS genes and haplotypes using well known Glu-A3, Glu-B3 and Glu-D3 gene specific primers and to interlink their protein products by proteomic approaches in a wheat variety. A total of 36 LMW-GS genes and pseudo-genes were amplified including 11 Glu-3 gene haplotypes, designated as GluA3-13K and GluA3-22K (pseudogene) at Glu-A3 loci, GluB3-33K and GluB3-43K at Glu-B3 loci and GluD3-11K, GluD3-21K, GluD3-31K, GluD3-42K, GluD3-5K, GluD3-6K and GluD3-393K (pseudogene) at Glu-D3 loci. To determine the relationship between gene haplotypes and their protein products (to identify the corresponding LMW-GS proteins), we conducted N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS) analysis of the 17 LMW-GS spots separated by 2-DGE. Successfully, LMW-GS proteins of the Glu-3 gene haplotypes except pseudo-genes mentioned above were identified. This is the first report on comprehensive characterization of LMW-GS genes and their corresponding proteins and establishment of specific correspondence between each other in a single wheat cultivar. Our approach will be useful to understand the molecular basis of the LMW-GS and to study their contribution to the end-use quality of flour.

국립식량과학원에서 수발아와 붉은곰팡이병 등에 대한 내재해성이 강한 국수용 밀 품종 육성을 목적으로 1997년도에 금강밀과 은파밀을 인공교배 한 F1을 모본으로 하고 다시 금강밀을 부본으로 인공교배하여 SW97005 조합을 육성하였다. 경기도 연천에서 집단재배 후에 계통을 전개하여 초형과 수형이 양호하고 내한성이 강한 계통인 SW97005-B-26-5-4-1-1-1을 2005년과 2006년도 2년간 생산력검정을 거쳐 ‘익산319호’로 계통명을 부여하고, 2007년부터 3개년 동안 지역적응시험을 실시한 결과 내한성이 강하고, 수발아 저항성이며 붉은곰팡이병에 중도저항성이고, 수량이 많을 뿐만 아니라 국수가공적성이 우수하여 2009년 농작물 직무육성 신품종심의회에서 ‘수안밀’로 명명하였다. 그 후 국립종자원의 재배심사를 거쳐 2013년 종자산업법제55조에 따라 품종보호 등록 원부에 등록되었다. 수안밀의 파성은 III형으로 양절형이고, 엽색은 녹색이며 이삭은 방추형이고, 종자는 중간크기로 적색이다. 출수기와 성숙기는 밭상태 조건에서 각각 4월 25일과 6월 4일로 금강밀보다 1일 빠르고, 논상태에서는 각각 4월 23일과 6월 4일로 동일하다. 내한성과 수발아 저항성이 강하고 붉은곰팡이병은 중도저항성이다. 수안밀의 제분율은 금강밀보다 약간 낮고, 단백질 함량과 회분함량이 낮아 밀가루 색이 밝고 단백질적 특성은 국수용에 적합하다. 천립중은 43.7 g으로 금강밀보다 약간 적은 소립이며, 지역적응성 시험에서 밭상태 수량은 594 kg/10a, 논상태 수량은 563 kg/10a로 금강밀보다 3%와 8% 증수하였다.

Characteristics of agronomy, especially good yield productivity is very important in wheat (Triticum aestivum L.) as human food crop. Development of elite cultivar having good traits is required for food stability. In 2013, a new wheat line called Iksan370 has been developed by the National Institute of Crop Science, RDA. Iksan370 has hard red winter wheat characteristics and better features like resistance to biotic, abiotic stress and higher yield ability than Keumkang. Heading and maturing date were delayed about ten days and period to flowering date was shorter three or four days than Keumkang. Culm and spike length of Iksan370 were 95cm and 15.3cm, averagely. This means that culm and spike length were longer than Keumkang (79cm and 7cm, respectively). It showed higher milling rate (75.5%), protein content (13.5%) and SDS-sedimentation volume (77.5ml) than Keumkang. Genotypes of six genes, GBSSA, B, D, PPO18, Pina and Pinb, were represented that were similar to Keumkang, respectively. Average yield of Iksan370 in the preliminary trial test was 6.61 tons/ha in upland. We expect that yield productivity and farm income were increased by Iksan370.