The objective of this study is to measure the changes of regional cerebral blood flow(rCBF) and blood pressure(BP) in rats, following the intravenous injection of drug. The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure tranducer(Grass, USA) in anesthetized adult Sprague-Dawley rats for about 2 to 2 and a half hours through the data acquisition system composed of MacLab and Macintosh computer. The result of this experiment was as following 1. Poria increased the changes of rCBF in rats, significantly. 2. The rCBF of Poria was significantly decreased by pretreated propranolol, but not by pretreated L-NNA. 3. Radix Cynanchi Wilfordii decreased the changes of rCBF in rats, significantly. 4. The rCBF of Radix Cynanchi Wilfordii was not changed by pretreated propranolol and L-NNA. 5. Poria and Radix Cynanchi Wilfordii increased the changes of rCBF in rats, significantly. 6. The rCBF of Poria and Radix Cynanchi Wilfordii was significantly decreased by pretreated propranolol, but not by pretreated L-NNA. 7. Poria decreased the changes of BP in rats, significantly. 8. The BP of Poria was significantly increased by pretreated propranolol and L-NNA. 9. Radix Cynanchi Wilfordii did not change the BP in rats. 10. The BP of Poria and Radix Cynanchi Wilfordii did not change by pretreated propranolol but was increased by pretreated L-NNA. According to the result above, Poria and Radix Cynanchi Wilfordii have a different effect on each other, extract together. It is considered that the effect of Poria and Radix Cynanchi Wilfordii increased that of each drugs, the increasing rCBF of Poria and Radix Cynanchi Wilfordii is mediated by adrenergic β-receptor.
This study carried out to determine the physicochemical components of Cynanchi wilfordii Radix (CWR) fermented with Rhizopus delemar. The physicochemical components such as changes on moisture content, pH, total acidity, reducing sugars, and the contents of free sugars, free amino acid, cynandione A were investigated. The moisture content of fermented CWR was increased after 4 days of fermentation. The pH value of fermented CWR was decreased after 4 days of fermentation. The reducing sugars contents were in the range of 1.42-1.69%. The major free sugars were identified as rhamnose, glucose, fructose and sucrose. The content of free sugars was decreased during fermentation process of CWR while the glucose contents in 80% ethanol and water extracts were increased. In addition, the free amino acid increased significantly during fermentation. Finally, The content of cynandion A of the fermented CWR was highest at the level of 14.09 mg/g on the 8th day. These results are indicated that fermentation with Rhizopus strains could be used to increase the bioactivity of CWR and the fermented CWR can be used as a functional material and edible resource in the food industry.
본 논문은 국내 적하수오 및 백하수오 추출물의 항산화 능 및 항균활성을 구명하기 위한 목적으로 진행하였다. 각 하수오의 열수, 에탄올, 부탄올 추출물에 대하여, 일반성분 을 분석한 후, 항산화능에 대해서는 총 페놀함량 및 DPPH 라디칼 소거능을 측정하였으며, 항균활성에 대해서는 Staphylococcus aureus 및 Propionibacterium acne에 대한 최소생육저해농도(minimum inhibitory concentration, MIC) 및 생육저해환을 통하여 평가하였다. 적하수오 및 백하수 오 부탄올 추출물의 총 페놀함량은 각각 1,212.6 및 1,454.5 mg/g GAE로 다른 유기용매 추출물에 비해 유의적으로 높 은 함량을 보였다. 이에 비해 DPPH 라디칼 소거능은 열수 추출물에 비해 에탄올 추출물(89.0%), 부탄올 추출물 (88.9%)이 유의적으로 높은 값을 나타내었다. MIC 측정 결과, 적·백하수오 에탄올 추출물만이 0.8 mg/mL로 S. aureus와 P. acne에 대해서 항균효과를 나타냈다. 디스크 확산법 측정 결과, 모든 유기용매 추출물이 5 mg/disc 농도 부터 생육저해환을 형성하였으며, 농도가 증가함에 따라 생육저해환의 크기도 증가하였다. 적하수오 및 백하수오 에탄올 추출물의 조사균주에 대한 생육저해완의 직경은 10 mg/mL 농도에서 각각 8.9 및 9.2 mm를 나타내, 추출물 중 가장 우수한 항균활성을 나타냈다. 본 연구는 하수오 추출물이 항산화 효과 뿐만 아니라 피부 건강과 관련된 세균에 대해 항균활성을 보여줌으로써, 하수오 추출물이 피부 건강을 위한 천연 유래의 기능성 화장품 소재로도 활용될 수 있는 잠재적 가능성을 제시하였다.