The present experiments on cryopreservation were designed to examine the effects of solution toxicity, equilibration time and cell stages on the post-thaw survival of bovine IVF embryos. The oocytes were matured in vitro(IVM) for 24 hrs. in TCM-199 supplemented with 35 g /ml FSH, 10 g /ml LH, 1 g /ml estradiol-17 and granulosa cells at 39 under 5% in air. They were fertilized in vitro(IVF) by epididymal spermatozoa treated with heparin for 24 hrs., and then the zygotes were co-cultured in vitro(IVC) with bovine oviductal epithelial cells for 7 to 9 days. The bovine IVF embryos were exposed to the EFS solution in one step at room temperature, kept in the EFS solution during different period for toxicity test, vitrified in liquid nitrogen, and thawed rapidly. 1. after the bovine blastocysts were exposed to EFS solution for 2 min. at room temperature and then they were washed in 0.5 M sucrose solution and TCM-199, they were cultured to examined cryoprotectant induced injury during exposure, Most of the embryos(95.0%) developed to reexpanded blastocoels. However, when the exposure time was extended to 5 and 10 min, these development rates dropped dramatically in 5 min. (69.5%) and 10 min. (47.4%), respectively, 2. When the bovine IVF embryos were vitrified in EFS solution after the equilibration for 1 and 2 min. exposure, The embryos to have reexpanded blastocoels following thawing, washing and culture processes were found to he 82.6 and 73.9%, respectively. However, when the exposure time was extended to 3 min, this survival rate dropped to 18.2%. The optimal time for equilibration of bovine IVF blastocysts in EFS solution seemed to he 1~2 min. 3. When the bovine IVF embryos were equilibrated for 1 min. the significantly (P<0. 05) higher post-thaw survival rates were obtained from the embryos of blastocyst stage(81.3%) than morulae stage(5. 1%). The optimal cell stage for viterification with EFS solution proven to he blastocyst stage in bovine IVF embryos. 4. The number of blastomeres of blastocyst stage was examined with nuclear staining with Hoechst 33342 during 7 to 9 days post-insemination. The cell counts of frozen bovine IVF embryos were found significantly(P7.5 and those of the fresh embryos 76.67. 1, which were cultured in the sarne period and conditions as frozen embryos.

This experiment was carried out to investigate the effects of the final cutting time and cutting height on the winter survival, regrowth, and carbohydrate reserves in stubble, and early spring yield of orchardgrass-dominated pasture before and after the w

본 시험은 사초용(飼草用) 호밀의 파종기와 파종량이 사초(飼草)의 생육특성, 월동성 및 수량에 미치는 영향을 구명(究明)하기 위하여 서울대(大) 농대(農大) 사초시험포장(飼草試驗圃場)에서 수행되었다. 본 시험에서 호밀의 파종기(播種期)를 9월 8일, 9월28일, 10월18일로 하고 ha당 파종량(播種量)을 80, 130 및 180kg로 하여 9처리(處理) 3반복(反復) 난괴법으로 실시하였다. 호밀의 초장(草長)은 파종기(播種期)에 의하여 영향을 받지 않

본(本) 시험(試驗)은 제경법(蹄耕法)을 이용(利用)하여 산지초지(山地草地)를 조성(造成)할때 파종시기(播種時期)가 목초(牧草)의, 잔존(殘存), 건물생산(乾物生産) 및 이용률(利用率) 등에 미치는 영향을 조사하기 위하여 1982~'84년에 걸쳐 고령지시험장에서 수행(遂行)되었으며 그 결과(結果)를 요약하면 다음과 같다. 1. 목초(牧草)의 정착율(定着率)은 화본과의 경우 4월(月) 15일(日), 5월(月) 15일(日), 8월(月) 1일(日

Post-thawed larval rearing in Pacific oyster Crassostrea gigas was performed to investigate the survival rate with time course in three kinds of larvae cryopreserved. The highest survival rate and larval activity index (LAI) of post-thawed larvae were obtained from the permeation in 0.2 M sucrose and 2.0 M ethylene glycol (EG) at －1℃/min in freezing speed showing the survival rates just after thawing of 63.8% in trochophore, 84.1% in D-shaped veliger and 56.3% in early umbo veliger. In post-thawed larval rearing with food supply, the larvae lasted their lives until 24 hours in trochophore, 75 hours in D-shaped veliger and 57 hours in early umbo veliger. The results suggested that each larval stage post-thawed revealed no more further development to subsequent respective stage.

Epicotyl grafting in one of the most efficient propagation methods for walnut trees. We have analyzed the scion factors that may influence the survival rate of the grafts. The optimum time for the collection of scions was turned out to be between January