간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
2010 한국응용곤충학회 심포지엄 및 추계학술발표회 (2010년 10월) 172건
21.
2010.10
구독 인증기관·개인회원 무료
Although DNA barcode coverage has grown rapidly for many insect orders, there are some groups, such as scale insects, where sequence recovery has been difficult. However, using a recently developed primer set, we recovered barcode records from 373 specimens, providing coverage for 75 species from 31 genera in two families. Overall success was >90% for mealybugs and >80% for armored scale species. The G·C content was very low in most species, averaging just 16.3%. Sequence divergences (K2P) between congeneric species averaged 10.7%, while intraspecific divergences averaged 0.97%. However, the latter value was inflated by high intra-specific divergence in nine taxa, cases that may indicate species overlooked by current taxonomic treatments. Our study establishes the feasibility of developing a comprehensive barcode library for scale insects and indicates that its construction will both create an effective system for identifying scale insects and reveal taxonomic situations worthy of deeper analysis.
22.
2010.10
구독 인증기관·개인회원 무료
DNA barcoding uses a 650 bp segment of the mitochondrial cytochrome c oxidase I (COI) gene as the basis for an identification system for members of the animal kingdom and some other groups of eukaryotes. PCR amplification of the barcode region is a key step in the analytical chain, but it sometimes fails because of a lack of homology between the standard primer sets and target DNA. Two forward PCR primers were developed following analysis of all known arthropod mitochondrial genome arrangements and sequence alignment of the tRNA-W gene which was usually located within 200 bp upstream of the COI gene. These two primers were combined with a standard reverse primer (LepR1) to produce a cocktail which generated a barcode amplicon from 125 of 141 species that included representatives of 121 different families of Hexapoda. High quality sequences were recovered from 79% of the species including groups, such as scale insects, that invariably fail to amplify with standard primers. The current results show that primers designed to bind to highly conserved gene regions upstream of COI will aid the amplification of this gene region in species where standard primers fail and provide valuable information to design a primer for problem groups.
23.
2010.10
구독 인증기관·개인회원 무료
The family Bostrichidae contains 9 subfamily and about 580 species. Almost all have adapted to xylophagy, living mainly in sapwood rich in cellulose and many species are considered as serious and onerous technical pests - with increasing export of wooden articles and containers increases also the frequency of introduction of these beetles to various parts of the world, including those of temperate or cold climate (Borowski and Wegrzynowicz, 2007). In Korea, Up to days, 7 species of 5 genera of the family Bostrichidae have been recorded. We added two species, Dinoderus speculifer Lesne, 1895 and Xylothrips pekinensis (Lesne, 1902 - Comb. nov.). And Xylothrips cathaicus Reichardt, 1966 is synonymized with Xylothrips pekineisns (Lesne, 1902 - Syn. nov.). Borowski and Wegrzynowicz (2008) added Xylothrips pekinensis to the Japanese fauna with a photo and mentioned on the possibility of the distribution in the Korean peninsula, but this species had been already recorded to the Japanese fauna by Iwata and Kusakabe (2002).
24.
2010.10
구독 인증기관·개인회원 무료
본 연구는 국립수목원에서 수행 중인 우리나라 자생 산림생물에 대한 분포조사 를 통해 특정종별 보전 및 자원화를 위한 기초 자료 확보를 목적으로 수행되었다. 조봉은 해발 1,182m로 강원도 양양군 서면과 현북면에 위치하며 미천골자연휴양 림과 인접하여 있다. 식생은 소나무림과 신갈나무림이며, 지형적으로 계곡이 잘 발달되어 있으나, 현재까지 정확한 곤충분포상 등 기초조사가 이루어지지 않은 지 역 중 하나이다. 금번조사는 2009년 4월부터 9월까지 5회에 걸쳐 주간채집 및 야간조사를 병행 하여 실시하였으며, 산림지역에 우점분류군 중 하나인 나비목 곤충을 중점적으로 조사하였다. 본 조사에서 확인된 나비목 곤충은 30과 258종 715개체로 나타났다. 계절별로는 6월에 117종 249개체가 7월에 122종 254개체 등으로 조사되어 다른 시기에 비해 높은 종다양성을 보였다. 분류군별로는 밤나방상과(Noctuoidea)가 3 과 57종으로 가장 많은 종들이 확인되었으며 그 다음으로 명나방상과(Pyraloidea) 가 3과 53종으로 나타나 종다양성이 높은 분류군으로 확인되었다. 이와같이 조사 된 분포상 정보는 산림생물종의 보존 및 자원화를 위한 기초자료와 향후 기후변화 등에 의한 밀도변동 추이의 경향 파악 등에 활용될 것으로 기대된다.
25.
2010.10
구독 인증기관·개인회원 무료
Yang Seop Bae, Jong Kyun Park, Kyu Tek Park, Dong Pyeo Ryu, Do Young Chae, Young Don Joo, Le Xuan Vi, Jin Young Park
Cambodia covers an area of 181,035 sq km, among them, forests cover 53 percent, so there are abundant Insect diversities. Collecting expeditions of Insect were conducted several times from 2009 to 2010 (09. 7.2-7.11, 10.16-22, 12.25-12.30, 10. 4. 30-5.6) in Cambodia sponsored by Natioanl Biological Resources, Korea. Central Cadamon Protected Forest (N 11 41' 13" E 103 26' 31.3"), Seima Biodiversity Conservation Area (N 12° 08' 20.8", E 106 55' 2.7") and Prov. Pursat, Roveang (N 12° 19' 06.5", E 103° 3' 03.8") were main area surveyed by pitfall trap, light trap and sweeping, searching etc. Numerous individuals were collected and more than 150 species belonging to 40 families were founded, among them, there were lots of new and new recorded species. Among Cambodian insects, species of Lepidoptera, Coleoptra and Hymenoptera were preferentially introduced in here. The photos for species of dominant, rare, new and new recorded were provided, and also collecting sites and activities were presented.
26.
2010.10
구독 인증기관·개인회원 무료
The genus Lilioceris Reitter, 1912, containing generally thirty species of two subgenera, is distributed Oriental regions (Gressit and Kimoto, 1961). Seven species were recorded in Korea (Lee and An, 2001). Many of them are important pests on medicinal plants and garden flowers (lily, yam, etc). There have been a few studies on the adult taxonomy of Korean Lilioceris (Gressitt and Kimoto, 1961; An et al., 1986; An and Kwon, 1995, An, 1995, 1996, 1998, Lee and An, 2001). Although the main damage to the plants is caused by their larvae, nothing is known about the immature stages of Korean Lilioceris. L. (L.) ruficollis is associated with the host plants of Dioscorea (Dioscoreaceae), especially D. septemioba which is a well-known medicinal plant in Korea. In the present paper, detailed descriptions and illustrations of the immature stages of L. (L.) ruficollis are provided for the first time. Some remarks on their biology are also given.
27.
2010.10
구독 인증기관·개인회원 무료
The first checklist of the North Korean insects was presented by Zhu (1969), and it included 34 species of Tortricidae. From the early 1970s to the late 1980s, the Hungarian Natural History Museum (HNHM) had conducted zoological expeditions to North Korea under cooperative scientific research agreements (Park & Lee, 1992). These expeditions added significant information to our knowledge of the fauna of North Korea, including a large numbers of Lepidoptera. Based on the entomological collection of Hungarian Natural History Museum, Budapest, we report three species of Tortricidae (Lepidoptera), Ancylis melanostigma Kuznetsov, Eucoenogenes japonica Kawabe, and Eucosma glebana (Snellen) for the first time from North Korea. Photos of adults and the genitalia of the newly recorded species are provided with brief comments on the distribution. Also taxonomic accounts for the species are given.
28.
2010.10
구독 인증기관·개인회원 무료
Due to the very small body size of spider mite, it is difficult to prepare DNA extraction simultaneously with slide samples from a single individual. Here we developed non-mashed DNA extraction method from a single spider mite to apply for molecular as well as morphological identification. Total genomic DNA was isolated from a single female adult using Genomic DNA extraction kit without the sample homogenization. DNA content of a single spider mite was 60-90 ng, which is sufficient for the PCR analysis. However, the quantity of extracted DNA and quality of the cuticle sample were dependent on the incubation time into the lysis buffer. Our results suggest that non-mashed DNA extraction method would be useful for the identification of very small mites as well as insects at the levels of DNA and morphology.
29.
2010.10
구독 인증기관·개인회원 무료
As a member of fungus gnats, the genus Bradysia can be the pests on many kind of agricultural crops, including mushroom farming. Feeding on healthy roots of crops, they can also spread root rot diseases. In Korea Bradysia difformis is known as major pest in greenhouse crops (carrot, cucumbers, garlic, melon, potatoes, radish, strawberries, sugarbeet, sweetpepper, sweetpotato, etc). Besides Bradysia difformis, we report six Bradysia species for the first time in Korea.
30.
2010.10
구독 인증기관·개인회원 무료
Spider mites are one of major pests in cultivations of various ornamental plants and also important in plant quarantine service. Due to the very small body size and high similarity within the Genus the identification of species is difficult even at the microscopic observation. To identify 5 major species (Tetranychus cinnabarinus, T. urticae, T. phaselus, T. kanzawai and T. truncatus) in the Genus Tetranychus at the molecular level, we designed 4 species-specific primer sets using nucleotide sequences of the internal transcribed spacer (ITS2) region in the nuclear ribosomal repeat unit. At the PCR diagnosis of extracted genomic DNAs of 5 species using each primer set, specific primers of both T. phaselus and T. truncates were species-specific to their own species samples. However, specific primer set of T. urticae detected T. cinnabarinus as well as T. urticae. Specific primer set of T. kanzawai detected T. truncates as well as T. kanzawai, even though detection intensities were lower in non-target species.
31.
2010.10
구독 인증기관·개인회원 무료
DNA ‘barcoding’ has potential applications in insect pest monitoring and quarantine since large numbers of DNA sequences for insect species identification have been reported in recent years. However, the exact number of relevant COI sequences in public databases such as NCBI (http://www.ncbi.nlm.nih.gov/) is not readily available. The IMGD (Insect Mitochondrial Genome Database; http://www.imgd.org) contains 162,847 partially sequenced mitochondrial gene entries originated from 35,067 hexapod species and makes it possible to check whether DNA sequences have been previously reported or not for certain insect species. In this study, we applied the IMGD to establish baseline data for the forest pest insects in Korea, before constructing a DNA barcode system. Retrieving data from the IMGD, we recognized that DNA sequences were already available for 73 of 259 species known as forest pest insects in Korea. Most of the 73 species with DNA sequences are common pests worldwide but there maining 186 species are endemic to the Eastern Palearctic region. Based on these data, we are proceeding to construct a Korean Forest Insect Pest DNA barcode database.
32.
2010.10
구독 인증기관·개인회원 무료
The genus Linnaemya Robineau-Desvoidy (Diptera: Tachinidae: Linnaemya) is one of the largest genera of the subfamily Tachininae including 149 recognized species worldwide. We reviewed Korean species of the subgenus Ophina belonging to the genus Linnaemya. As a result, we recognized three species, L.(Ophina) microchaetopsis Shima, L.(Ophina). picta (Meigen) and L.(Ophina) zachvatkini Zimin. Among them, the latter two species are reported for the first time from Korea. They belong to the haemorrhoidalis species group of the subgenus Ophina. We provide a generic key, descriptions and illustrations of the species.
33.
2010.10
구독 인증기관·개인회원 무료
Goniozus Förster which is belonging to the subfamily Bethylinae (Hymenoptera: Bethylidae), are one of the large genera, and consist of approximately 170 species worldwide. Most members of Goniozus are ectoparasitoids of immature stages of microlepidopteran families, mainly Gelechiidae, Pyralidae and Tortricidae. As a results of this study, a total of five species of the genus Goniozus are recognized in Korea, which includes two new species, two newly recorded species and a previously recorded species. Diagnosis and illustrations for each species are presented.
34.
2010.10
구독 인증기관·개인회원 무료
As an natural enemy against the Monochamus alternatus Hope and M. saltuarius Gebler (Coleoptera: Cerambycidae), the vector species of the pine wood nematode (Bursaphelenchus xylophilus (Steiner & Buhrer), Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is recognized for the first time in Korea. The family Bothrideridae is also reported for the first time in Korea. We provide the diagnosis, illustrations, biological information, and the host insects of D. helophoroides (Fairmaire).
35.
2010.10
구독 인증기관·개인회원 무료
We present the first comprehensive cladistic analysis of Miridae, the plant bugs, based on analysis of 3935 base pairs of mitochondrial (16S, COI) and nuclear (18S, 28SD3) DNA for 91 taxa in seven subfamilies. We analyzed the data using maximum likelihood (ML), maximum parsimony (MP), and Bayesian inference (BI) phylogenetic frameworks. A Bayesian relaxed molecular clock was used to examine divergence times, and ancestral feeding habits were reconstructed using parsimony and a Bayesian approach. Clades recovered in all analyses are as follows: Cimicomorpha, Miroidea, and Miridae; Bryocorinae: Bryocorini; Stenodemini; Mirinae; Deraeocorinae (Clevinemini + Deraeocorini); Cylapinae; Isometopinae; Bryocorinae: Dicyphini; Orthotylini; Phylinae (Phylini + Pilophorini), and Phylinae is sister-group to all the remaining mirid taxa. These results are largely congruent with former hypotheses based on morphological data with respect to the monophyly of various subfamilies and tribes however, our results indicate that the subfamily Bryocorinae is not a monophyletic group as the two tribes, Dicypini and Bryocorini, were separated in our phylogeny. Ancestral feeding state reconstructions based on Bayesian and parsimony inference were largely congruent and both reconstructed phytophagy at the root of the Miridae.
36.
2010.10
구독 인증기관·개인회원 무료
The genus Gonioctena Chevrolat is distributed in the Holarctic and Oriental regions, with more than 80 species. Many species of the genus have great similarity in external morphology and coloration, often cause misidentifications. Thus, the shape of the aedeagus is mainly used to distinguish closely related species. Fifty-four species, more than half of the known taxa, occur in northeastern Asia, including 18 species in the Russian Far East, 29 in China, 11 in Korea, and 18 in Japan. Among these, G. gracilicornis Kraatz (type locality: Amur) is widely distributed in Russia, China, Mongolia and Korea. However, extensive investigation on Gonioctena in Korea revealed that the Korean population of G. gracilicornis differs from that of the Russian by the coloration, the puncturation of pronotum, and the shape of aedeagus. The purpose of this study is to describe a new species of Gonioctena that was previously misidentified as G. gracilicornis. A key to Korean Gonioctena, distribution, and host plant are also provided.
37.
2010.10
구독 인증기관·개인회원 무료
The genus Parapammene Obraztsov, 1960 belonging to the subfamily Olethreutinae, family Tortricidae includes very small to small moths. The genus is characterized by the followings: forewing with median fascia and speculum, and without whitish stria below apex; tegumen of male genitalia being a broad band with the top emarginate laterally; seventh stenite of female genitalia, excavated in posterior edge (Horak, 2006). This genus comprising about 16 species in the world (Brown, 2005). In palaearctic region, there are about 10 known species, 5 species are recorded from Oriental region, and one species in Australian region (Komai, 1999). Comprehensive reviews of genus Parapammene Obraztsov have been given by Obraztsov (1960), Danilevsky and Kuznetzov (1968), and Razowski (1989) and Komai (1999). The following character states support the monophyly of Parapammene (Komai, 1999): 1) ductus bursae with a narrow, band-like sclerite; 2) seventh sternite of female a convex (not flat) plate; 3) S2 without anterolateral processes. In this study, the genus Parapammene Obraztsov are reported for the first time from Korea, with the first record of parapammene selectana (Christoph, 1881), P. petulantana (Kennel, 1901) and P. aurifascia Kuznetzov, 1981. Morphological characters of this genus are redescribed with illustrations of adults, male and female genitalia.
38.
2010.10
구독 인증기관·개인회원 무료
The genus Xorides is a large group xoridine genus was firstly described by Latreille in 1809 based on Ichneumon indicatorius. The world fauna of Xorides have comprised of 147 described species(Yu et al., 2005). Among them, 48 species have distributed in Eastern Palaearctic region(Yu et al., 2005) and only one species, Xorides jezoensis, was recorded from Korea (Uchida,1955). Most species are specialized parasitoids of eight coleopteran families (Anobiidae, Bostrichidae, Buprestidae, Cerambycidae, Chrysomelidae, Curculionidae, Elateridae, Melandryidae), three hymenopteran families, two lepidopteran families, one dipteran family(Yu et al., 2005). Also, emerge from larva as ectoparasitoids(Sharifi & Javadi, 1971) and idiobiont(Chrystal & Skinner, 1931). This genus is similar to genus Odotocolon but mandible without two teeth; female flagellomere curved with peg like setae. In this study, we found two newly recorded species, Xorides konumensis(Uchida, 1928) and Xorides sapporensis(Uchida, 1928) from Korea.
39.
2010.10
구독 인증기관·개인회원 무료
In the tribe Macrosiphini (Hemiptera: Aphididae), we discovered two new records in Korea, Capitophorus formosartemisae (Takahashi, 1921) and Hyperomyzus lactucae (Linnaeus, 1758) collected on Artemisia princeps and Sonchus oleraceus, respectively. They are illustrated and described, based on the macerated slide specimens of the apterous viviparous females.
40.
2010.10
구독 인증기관·개인회원 무료
네발나비과 세줄나비속 나비는 열대 및 아열대지방에 153여종이 분포하며, 한 국에는 11종이 기록되었다. 나비류의 분류학적 연구는 성충의 날개무늬 패턴을 위 주로 형태연구가 주를 이루어왔다. 세줄나비(Neptis)속 역시 날개무늬패턴의 형태 적 유사성이 높다. 국내의 세줄나비의 종들 가운데는 종간 형질의 분리가 다소 모 호하거나, 중복되는 경우가 있어 왔다. 따라서 이번 분자분류학적 연구를 통해서 세줄나비속 국내종에 대한 기존 종 인식이 정확한 것인지를 재검토해 보고자 하였 다. 이번 연구에서는 기존의 형태분류를 통해 확증된 11종 33개체에 대한 DNA 바 코드를 분석하였으며, NCBI의 Blast search를 통해 확보된 국외산 3종 22개체를 실험에 추가 하였다. 그 결과, COI유전자 서열 차이는 0~16.2%를 나타내었고, 종 내 서열 변이율은 0.0~1.14%였으며, 종간 서열차이는 3.3~16.2%였다. 이로 볼 때, 1.14~3.3%의 Barcoding gap이 형성되는 것을 확인할 수 있었다. 따라서, Divergence threshold approach를 통해 한국산 세줄나비속의 종들은 확실히 구분 되었으며, Clade based approach에서도 각 종들의 개체들은 Barcoding gap을 반 영하면서 종별로 묶이는 것을 확인할 수 있었다. 이 결과는 기존의 형태 분류와 100% 일치하였으며, 결국 한국산 세줄나비속에 대한 종 동정에 있어 DNA 바코드 의 활용적 가치가 높은 것으로 나타났다
