Whole grain wheat bread and bakery products is highly considered as a functional food due to the high amount of vitamins, minerals, and dietary fiber in whole wheat flour. However, as the lower end-use properties of whole wheat products limit consumer selection, it is required to measure the functionality of whole wheat flour. Thus, it is observed the fermentation properties of bread dough from the composite flour of whole grain and white wheat flour. Whole grain cv. Chokyeong developed in the NICS was ground using a hammer mill with 0.5 mm sieve. The composite flour was prepared by substituting wheat flour with whole grain flour at 15, 30, 45% levels. Breads were prepared using the 100 g composite flour according to a basic straight-dough mehtod (AACC 10-10A) with slight modification. A rheofermentometer was used to measure the maximum dough height (Hm) and the maximum gas formation (H’m) during fermentation. Also, total CO2 production was recorded. The Hm decreased from 38.8 to 27.5 (mm) with increasing whole wheat flour from 15 to 45%. Whereas, the H’m was increased from 61.7 to 60.8 (mm) with whole wheat flour increment. Total CO2 production was also decreased with increasing whole wheat flour ranging 1312∼1164 (ml). The fermentation results could be useful to partly predict the effect of substituting whole wheat flour on the end-use quality of pan-breads.

Plants are known to have homeostatic cellular mechanisms to control the concentration of heavy metal inside the cell. We tried to retrieve rice RING finger protein genes, which are believed to regulate substrates via ubiqitinations, related to metal ions detoxification mechanisms. A total of 48 rice RING finger proteins were randomly selected and then examined for their expression patterns as exposed to cadmium and arsenic treatments. We discovered a RING finger protein gene that was significant up-regulated against both treatments and then named Oryza sativa heavy metal induced 1 (OsHMI1). We tested subsequently OsHMI1 expression patterns against to salinity, dehydration, cold, heat stress and phytohormones treatments. In addition, we evaluated its subcellular localization and determined E3 ligase activity. The interaction partner proteins were screened via yeast-two hybridization. These results might shed further light on the understanding of homeostatic cellular mechanisms to control heavy metal detoxification via protein degradation in plants.

Discovery, identification, and informatics of low molecular weight peptide are extensively rising in the field of proteomics research. In this study, we analyzed protein profiles to discover peptide based biomarker for twelve different soybean seeds with three different agronomic types using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). For optimization of SELDI-TOF MS in soybean seed proteome analysis, four different extraction buffers were tested with urea solubilization buffer, thiourea/urea solubilization buffer, phenol extraction buffer, and modified trichloroacetic acid (TCA)/acetone precipitation/urea solubilization extraction buffer. Two different type of ProteinChip arrays, cation exchange (CM10) and anion exchange (Q10), applied to profile peptides. Among the four different extraction buffers, phenol extraction was selected to protein extraction methodology. Numbers of detected peak cluster in twelve soybean seeds were 125 at CM10 and 90 at Q10 array in the mass range from 2 to 40 kDa. Among them, 82 peak clusters at CM10 and 33 peak clusters at Q10 array showed significantly different peak clusters at p<0.00004 (CM10) and p<0.00005 (Q10) among twelve different soybean cultivars. Moreover, 29 peak clusters at CM10 and 17 peak clusters at Q10 array were detected in all cultivars as an ‘universally existed peptide’. In comparison with three different agronomic types, total of 55 peak clusters (CM10) and 23 peak clusters (Q10) were significantly different peak clusters at p<0.00004 and p<0.0001, respectively. In these probability levels, soybean seeds were well discriminated into different cultivar and different type with each other. Also we could find several specific peptide biomarkers for agronomic type.

Recently, proteome analysis is becoming a powerful tool for the functional characterization of plants. Due to the availability of vast nucleotide sequence information and based on the progress achieved in sensitive and rapid protein identification by mass spectrometry, proteome approaches open up now perspectives to analyze the complex functions of model crop species at different level. In this study, we have N-terminal sequencing data for the 100 embryo and 53 seed proteins of rice separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) were collected and systematically organized for a protein sequence data-file. An attempt was made to link the embryo proteins of rice to DNA sequences for understanding their functions. One hundred proteins of the 700 spots were detected in the embryo using 2-DE gels whereas we used micro sequenced. Of these, 28% of the embryo proteins were matched to DNA sequences with known functions, but 72% of the proteins were identified to be unknown functions as previously reported by Woo et al.,. In addition, twenty-four spots of protein with 100% of homology and nine with over 80% were matched to ESTs (expressed sequence tags) after expanding the amino acid sequences of the protein spots by Database searches using the available EST databases of rice at the NCBI (http://www/ncbi.nlm.nih.gov/) and DDBJ (http://www.ddbj.nig.ac.jp/). Also, a total of 53 proteins out of 700 protein spots separated on the 2-DE gels were analyzed by the peptide mass fingerprinting method (MALDI-TOF/MS). High-quality mass spectra suitable for peptide mass fingerprinting were obtained from 41 spots. Using the ESI-Q-TOF/MS, however, we were able to identify 53 seed proteins of rice, including 12 proteins not registered in database. The rapid expansion of DNA sequence databases to the utilization of EST now provides the whole or partial gene sequences of model organisms, and the recent advances in protein micro-characterization by mass spectrometry allow the possibility of linking these DNA sequences to the proteins in functional complexes. Proteome Database of rice is updated, and is available on the World Wide Web at http://gene64.rda.affrc.go. This work shows that the proteome analysis could be a useful strategy to link the sequence information to the functional genomics.

Soybean [Glycine max (L.) Merr.] protein is excellent nutritional factors and is widely used for human and animal feed in the world. Glycinin(11S globulin) and ß-conglycinin(7S globulin) account for storage protein from 70% to 80% in soybean seed. ß-conglycinin is composed of α’, α and ß subunits, encoded by the genes Cgy1, Cgy2, and Cgy3, respectively. β-conglycinin protein exhibits poor nutritional and food processing properties. Lipoxygenase is responsible for the beany flavor and is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to select F2 seeds with lipoxygenase-free and low content of ß-conglycinin for breeding of high quality soybean cultivar. A total of 582 F2 seeds were obtained from the cross of 10F1(lipoxygenase-free, normal content of 7S protein) and T311(lipoxygenase-present, low content of 7S protein). Lipoxygenase and 7S proteins in mature seeds were detected by SDS-PAGE. The segregation ratio of 3 : 1 for Shr locus (418 Shr_ : 164 shrshr) were observed. This result shows that the Shr locus is controlled by a single gene. Among 164 shriveled seeds, 6 F2 seeds with lipoxygenase-free, α’ subunit-free, and low content of α and ß subunit were selected. Among 418 normal seeds, 6 F2 seeds with lipoxygenase-free, α’ subunit-free, and low content of α and ß subunit were selected. These results will be used in breeding program for improving high quality soybean cultivar.

A New Six-rowed Naked Barley Cultivar “Ganghocheong” with High anthocyanin content and green aleurone the cross between Iri26 and Sagukgwa67 by the National Institute of Crop Science, RDA in 2009. An elite line HB14190-B-61-3-2-3-3-5-1 was selected in 2004 and designated as ‘Iksan 92’. It showed good agronomic performance in the regional yield trials (RYT) from 2007 to 2009 and was released with the name of “Ganghocheong” possessing high anthocyanin content and green lemma non-waxy endosperm. The average heading and maturing dates of “Ganghocheong” were April 20 and May 29 in paddy field, which were two days earlier and same than those of the check cultivar ‘Saessalbori’ respectively. The new cultivar, “Ganghocheong”, had 81cm of culm length and 6.0cm of spike length. It showed 702 spikes per m2, 60 grains per spike, 30.0 g of 1,000-grain weight, and 794 g of test weight. “Ganghocheong” showed better resistance to BaYMV (Barley yellow mosaic virus) than those of the check cultivar, ‘Saessalbori’. Its average yield of the pearled grain in the regional yield trial was 4.02 MT/ha in paddy field, which were 1% lower than that of the check cultivar, respectively. Anthocyanin content was 122.1㎍/g that was higher 2 times than that of zasoojeongchalssalbori. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.

This study investigated saponin contents according to collection area, seed size and seed color in 293 Korean soybean landraces. Results showed that total group A was 149.8 to 1279.0㎍/g with an average of 484.9㎍/g while total group B saponin content was 2160.1 to 7868㎍/g with an average of 3670㎍/g. Total groups A and B saponin content were ranging from 2502.7 to 8763.9㎍/g with an average of 4154.8㎍/g. The landraces with high group B saponin content were IT226841, IT226761, IT226844 and IT226826. The landraces with low group A saponin content were IT228580, IT228319, IT228308 and IT226841. According to collection areas, the highest amount of saponin content was observed in the landraces collected from North Korea. High total of saponin content was showed by seeds from Gyeonggi-do, followed by Chungcheongbuk-do, Gangwon-do, Gyongsangbuk-do, Chungcheongnam-do Jeollabuk-do and Jeollanam-do. According to seed size, small seeds have the higest saponin contentfollowed by the medium and large size of seeds. For seed color, there was no significant difference in saponin content. The total of saponin content was postively correlated with group A saponin, group B saponin and total of saponin cntent. Among each components, group A saponin showed postive correlations with total of saponin and group B saponin content.

A New six-rowed Naked Barley Cultivar “Nurichal” with good coking quality was developed by cross with HB88154-B-25-1 line and Hinchalssalbory by the Winter Cereal and Forage Crop Research Div. NICS, RDA in 2010. HB15305-B-B-31-2 was selected in 2006 and designated as ‘Iksan 95’. It showed good agronomic performance in the regional yield trials (RYT) from 2008 to 2010 and was released with the name of ‘Nurichal’ possessing good water absorption and expansion. The average heading and maturing dates of ‘Nurichal’ were April 24 and May 30 in paddy field, which were two day and one day later than those of the check cultivar ‘Saechalssalbori’ respectively. The new cultivar, ‘Nurichal’, had 82cm of culm length that was 5cm shorter than that of ‘Saechalssalbori’, and 4.0cm of spike length. It showed 774 spikes per m2, 63 grains per spike, 25.0 g of 1,000-grain weight, and 793 g of test weight. ‘Nurichal’ showed a little weaker winter hardiness than those of the check cultivar, ‘Saechalssalbori’ and showed similar lodging resistance with check cultivar. It sowed stronger resistance to barley yellow mosaic virus (BaYMV) compare to check cultivar. Grain shape was small and round. Protein content was 10.3% and β-glucan content was 6.8%. Its average yield of the pearled grain in the regional yield trial was 3.84 MT/ha in paddy field, which were higher 2% than those of the check cultivar. Its water absorption was similar and expansion was higher than ‘Saechalssalbori’. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.

Cereal seeds, sorghum, foxtail millet, hog millet, adlay, and corn are traditionally used as health assistant as well as energy supplying food in Korea. While beneficial phytochemicals to human have revealed in cereals, the information on peptides from cereals is far less accumulated than major reserve protein. Here, we analyzed peptide profiles using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF MS) in cereal seeds for construction of peptide information and attempted to develop peptide biomarkers for cereal identification. To optimize the analysis condition of SELDI-TOF MS, the effect of dilution factor on binding affinity to protein chips was tested using CM10 and Q10 arrays. Peptide clusters were significantly different at the level of 0.01 p-value. Peak spectra were the most stable in 1:50 of dilution factor in both chip arrays. Numbers of detected peak of 5 cereal seeds were 131 in CM10 and 74 in Q10 array. Each cereal was grouped as a cluster and well discriminated into different cluster in the level of 0.01 p-value. Numbers of potentially identified peptide biomarkers are 11, 13, 9, 5 and 12 in sorghum, foxtail millet, hog millet, adlay and corn, respectively. This study demonstrates that each cereal seed have own distinguishable specific peptides although their function are not identified yet in this study. In addition, the proteomic profiling using SELDI-TOF MS techniques could be a useful and powerful tool to discover peptide biomarker for discrimination and assess crop species, especially under 20 kDa.

The application of next generation sequencing technologies allows us to discover the high levels of DNA polymorphism throughout a genome, e.g., single nucleotide polymorphisms (SNPs), and insertions and deletions (InDels). We performed whole-genome resequencing of a Korean rice cultivar (cv. Donganbyeo) and then obtained the sequences of covered 366,042,872 bp (96.63%) with average mapped read depth of 34.17 on 382,788,128 bp of the Japanese cultivar genome (cv. Nipponbare). We characterized the polymorphisms of 173,711 SNPs, 295,334 insertions and 40,642 deletions based on the comparison of both genomes. About 11.5% and 17.8% of the annotated total SNPs were presented in the regions of 1kb upstreams and genes, respectively. The annotated InDels in gene regions were similar with 15.5% insertion (4,588) and 15.9% (5,100) deletions, but not in 1kb upstream regions with 9.0% insertion (2,662) and 14.3% deletions (5,100). In addition, the Korea rice genome sequences were mapped on individual chromosome, resulted that SNPs were shown with different frequencies from each chromosome. The InDels distributions on individual chromosomes exhibited similar pattern as compared to those of SNPs. Some gene families such as NB-ARC (NB-LRR), F-box, RLK (serine/threonine protein kinase) and Zinc-finger (RING) for SNPs occurred the similar pattern with those of Arabidopsis. These results might be useful for better understanding the genome structure and genetic diversity of the Korean rice cultivars.

Kenaf (Hibiscus cannabinus) is an annual herbaceous plant of the family Malvaceae that has been planted in Africa for more than 4000 years and used as source of fiber, energy and feed stock. Also, kenaf seeds are good source for edible oil used for first class cooking oil and margarine production. The seeds can be used for lubrication, soap, paint and varnishes. This study was carried out to evaluate fatty acids variation among sixteen kenaf germplasm and gamma-ray induced mutants derived from Jinju and Auxu. Linoleic, oleic, and palmitic acid were the predominant fatty acids in all kenaf seed oils. The sixteen accessions showed a wide range of fatty acid compositions, spanning from 28.94 to 43.36% saturated, 56.64 to 71.05% total unsaturated, 15.52 to 46.85% monounsaturated, and 13.56 to 48.97% polyunsaturated fatty acids. The mutant lines derived from Jinju, significantly surpassed parental mean for all the palmitic and oleic acid. Also, the mutant lines derived from Auxu showed broad ranges of variation in oleic and linoleic acid and narrow ranges of variation in stearic and palmitic acid. The relative amount of monounsaturated fatty acids (MUFA) were increased at all the gamma-ray induced mutants. These results will provide a valuable information to assist parental selection of kenaf breeding.

Heterotrimeric G proteins, consisting of Gα, Gβ and Gγ subunits, play important roles in plant development and cell signaling. In Arabidopsis, in addition to one prototypical G protein a subunit gene, GPA1, there are three extra-large G proteins, XLG1, XLG2, and XLG3 of largely unknown function. Yeast two-hybrid library screening and in vitro protein pull-down assays revealed that XLG2 interacts with the nuclear protein RELATED TO VERNALIZATION1 (RTV1). A mutant XLG2 that lacks GTP binding does not interact with RTV1, suggesting the dependence of this protein interaction on the G-protein cycle. Electrophoretic mobility shift assays show that RTV1 binds to DNA in vitro in a non-sequence specific manner and that GTP-bound XLG2 promotes the DNA binding activity of RTV1. Overexpression of RTV1 results in early flowering. Combined overexpression of XLG2 and RTV1 enhances this early flowering phenotype, and elevates expression of the floral pathway integrator genes, FT and SOC1, but does not repress expression of the floral repressor, FLC. Chromatin immunoprecipitation assays show that XLG2 increases RTV1 binding to FT and SOC1 promoters. Thus, a Ca2+-dependent Gprotein, XLG2, promotes RTV1 DNA binding activity for a subset of floral integrator genes, and contributes to floral transition.

Barley, ranking fifth among all crops in the world today behind maize, wheat, rice and soybean, has been an important food source in many areas in the world. Moreover having high nutritional value. Current consumers have interest in nutrition and health. So, the interest in barley for other food and industrial applications is increasing. Noodles, which have been consumed largely in korea, are usually made from wheat flour, salt and appropriate amount of water. The objective of this study is to substitute wheat flour with barleys in order to produce new products of white salted noodles and to investigate the effects of substituting flours on quality characteristics of noodles. We investigated the Hunter colour values(lightness, redness, yellowness) of barley noodles. Lightness and yellowness were decreased and redness were increased in barley cooked noodles compare to wheat noodle. As compared to the wheat noodle, barley noodle at 10, 20, 30% substitution ratios had lower hardness, which decreased continuously with substitution levels. Springiness and cohesiveness were also lower than wheat noodles.

배추과 작물에 속하는 양배추(Brassica oleracea var. capitata)는 해외 시장에서 활발히 거래 되는 작물 중 하나로 고부가가치 수출용 작물로 육성하기에 적합한 경쟁력을 가지고 있다. 특히 중국, 인도, 동남아시아 국가들은 육종기술이 초기수준이고 종자수요량의 대부분을 수입에 의존하고 있으며 점점 내병성, 내서성 등의 내재해성과 고순도, 고품질의 형질을 갖춘 품종 욕구가 더해가고 있다. 이에 따라 우수한 품종이 해외 시장에서 보다 확대되기 위해서는 내병성과 같은 특징뿐만 아니라 순도 또한 매우 중요한 확인사항 중 하나이다. 현재 자가불화합성을 이용한 육성기술 보다는 품종 복제가 불가능하고 균일성이 100%에 이를 수 있는 세포질웅성불임성을 이용한 육성으로 그 방향이 전환되고 있는 실정이다. 그러므로 본 연구는 순도검정용 분자표지를 개발하여 일대 잡종종자의 순도를 검정하여, 최종적으로 F1의 상품가치를 감소시키는 자식체를 검정함으로써 고순도의 종자를 공급하고자 하며, 수출경쟁력이 강화되는데 그 목적을 두고 있다. 분자표지 개발 방법으로 NCBI에서 양배추의 EST 염기서열 정보로부터 30개의 SNP primer 조합이 200bp이 되도록 고안하였다. 개발된 SNP marker는 real-time PCR를 기반으로 한 HRM분석법을 이용하여 유전분석을 실시하였다. 본 실험에서는 아시아종묘의 양배추 주력 품종인 CT12, CT44, CT10, CT55, 대박나 총 5 품종에 대해 개발된 순도검정용 marker를 적용하였다. 그 결과 5 품종 모두 (CT12, CT44, CT10, CT55, 대박나)에서 양친과 F1을 구분할 수 있는 co-dominant형태의 marker를 각각 1개 이상 개발하였다. 개발된 marker를 이용하여 5품종의 순도를 검정한 결과 세포질웅성불임성을 이용하여 채종된 종자의 순도율은 100%로 확인되었다. 개발된 양배추 순도검정용 marker를 이용한 검정은 고순도의 종자공급을 할 수 있으며 고품질의 F1 품종을 생산하는데 활용하여 양배추 종자 시장의 수출증대와 농가소득증대에 기여할 것이다. 더 나아가 유연관계가 가까운 배추과 작물에도 적용할 수 있는 순도검정 marker로 매우 유용하게 활용할 수 있을 것이라 기대된다.

During the last decade, considerable progress has been made to understand the molecular mechanisms of M. grisea infection in rice plants and 10 rice blast R genes have been identified and characterized via map-based cloning methods. In case of rice germplasm, the genetic backgrounds of each germplasm accessions are not uniform and the evaluation for pathogenicity is difficult. To solve these problems, we applied the single resistance gene markers to rice germplasm accessions. A molecular survey was conducted to identify the presence of major blast resistance (R) gene in 363 accessions of Korea landrace rice germplasm. The results revealed that the resistance gene Pik-p (100%), Pib (98%), Pi-d(t)2 (98%) and Piz (76%) were widely observed in tested rice germplasm, but Pita-2, Pik and Pi39 gene were identified in less than 10 accessions. Most of landrace contain the four or five different resistant genes, but these results was not consist of field nursery screening. 13 accessions were shown the blast resistance in field nursery screening and Pik-p, Pib, Pi-d(t)2 and Piz genes were observed in these accessions. The evaluation results of blast resistance genes in rice germplasm will help in breeding of multi disease resistant varieties.

The need to develop work on conservation of crop genetic diversity is emphasized in change of agricultural environment, such as climate change, turn of dietary habits, use of cereal to bioenergy production. To secure provide the germplasm and to derive improve quality, customers demands was surveyed in 2011 and the results are as follows. In the questionnaire on genetic resources necessary in the future, Korean landrace was the highest as 23.8%, followed by genetic resources of international agricultural research institutions as 21.4% and genetic resources of foreign countries of 21.4%. Followed by 42.8% for genetic resources from foreign countries, response ratio were 10.7%, 9.5% and 9.5% for wild or wild-relative species, advanced cultivar or breeders’ line from foreign countries, and advanced cultivar or breeders’ line from Korea, respectively. In addition, consumers desired different genetic resources according to their institutes. Satisfaction on seed purity, germination rate and expected characteristic expression with the genetic resources was higher than normal by 87.5%, 88.9%, 84.2% of respondents, respectively. In order to improve the quality of conserved resources, it is necessary to select the resources having useful characteristics from the time of introduction, and to facilitate the evaluation of the preserved accessions in the near future. Efforts for expansion and opening of information on genetic resources are required.

The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assement of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 80 polymorphic and reproducible amplification fragments. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and expected heterozygosity (HE) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups, which was the landrace, moderate and wilde type, genetic distance (GD) value was 0.540. An average day of flowering time was 53 days with a range of 45 to 60 days. The everage od fruit length and width were 9.38cm with a range 2.1 to 23.6cm, and 3.51cm with a range 0.6 to 8.9cm, respectively. Molecular data were complemented with morphological measurements according to the descriptor list for the pepper collection of Bulgaria landraces germplasm.

Korean soybean variety Kwangan was transformed with coat protein (CP), helper component-proteinase (HC-Pro), and ABRE binding factor 3 (ABF3) genes using highly efficient soybean transformation system. Among these genes, CP and HC-Pro were transformed using RNAi technology. Transgenic plants with CP were confirmed for gene introduction and their expression using PCR, real-time PCR, RT-PCR, Southern blot, and Northern blot. To investigate the response of viral infection with CP, T1 plants were inoculated with SMV-infected leaves and confirmed the existence of mosaic symptom in both leaves and seeds. Two transgenic lines with CP were highly resistant to SMV with clear leaves and seeds while SMV-susceptible lines showed mosaic symptom with seed mottling. The transcript levels of T1 plants with CP were also determined by northern blot, suggesting that SMV-resistant T1 plants did not show viral RNA expression whereas SMV-susceptible T1 plants showed viral RNA expression. Currently, the response of viral infection with HC-Pro is investigating to produce SMV-resistant soybean transgenic plants, and the physiological experiment with ABF3 is also carrying out to produce drought-tolerant soybean transgenic plants.

Soybean (Glycine max, 2n = 2x = 40) is broadly distributed throughout East and South East Asia, and important crop as a source of protein, oil, food and animal feed. In order to better understand the morphological differentiation of soybean germplasm collected from China, Japan, Korea, Philippines, America, we analyzed the morphological variabilities among 629 soybeans with 11 morphological traits, such as growth type, leaflet, flower color, trichome, seed coat color, color inside seed etc. and measured the fatty acid composition. The result of the principal coordinates analysis (PCoA) based on the 11 morphological traits revealed diversity among all accessions. The PCoA separated the accessions into two main groups, each group with distinctive features. Among tested germplasm, the contents of five fatty acids were as follows: linolenic acid (2.8%－16.23%), linoleic acid (27.4%－56.6%), oleic acid (9.2%－35.0%), stearic acid (2.9%－8.8%), and palmitic acid (8.7%－17.1%). The fatty acid composition has not shown significant variation among all accessions. IT 22268 was the highest linolenic acid composition (16.2%), while IT 154687 was the lowest (2.8%). Forty three of 629 accessions showed the arachidic acid (0.5%-3.6%), which is the saturated fatty acid with a 20 carbon chain and is as a minor constituent of peanut oil (1.1%-1.7%). This result of this characterization served as reliable resources for detailed description and new functional plant breeding of soybean varieties.

Korean soybean variety Kwangan was transformed with ORE7 gene using highly efficient soybean transformation system. The gene is known to exhibit a delayed leaf senescence phenotype in Arabidopsis. To confirm phenotypic characterization of leaf senescence for non-transgenic (NT) and transgenic plants, we transplanted T1 transgenic lines 7, 9, 14 and 15 together with two negative controls (NT and EV) in greenhouse. As a result, line 15 showed dramatic phenotypic characterization of yield increase and senescence delay. In addition, to investigate the agriculture traits for transgenic plants with leaf senescence delaying, T2 transgenic lines and two negative controls were transplanted on GMO fields in Ochang and harvested T3 seeds (2010). Most transgenic lines showed higher total seed weigh than NT. Especially, total seed weight of line 15 was increased by about 180% and 120% compared with the NT and EV, respectively. Therefore, we carried out the second field experiments with T3 transgenic line 15 and NT in Ochang (2011). A total of 117 transgenic plants were divided into two groups, senescence delaying (64 out of 117 plants) and increased yield (53 out of 117 plants), by transcript level of ORE7 gene. Interestingly, among increased yield plants, total seed weight of each 7 plants were increased by more than 200% compared with NT.