Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited resources. In this study, we tried to optimize the experimental conditions for on-site detection of pathogens using a combination of ultra-fast convection polymerase chain reaction (cPCR), which does not require regular electricity, and nucleic acid lateral flow (NALF) immunoassay. Salmonella species was used as the model pathogen. DNA was amplified within 21 minutes (equivalent to 30 cycles of polymerase chain reaction) using ultra-fast cPCR, and the amplified DNA was detected within approximately 5 minutes using NALF immunoassay with nucleic acid detection (NAD) cassettes. In order to avoid false-positive results with NAD cassettes, we reduced the primer concentration or ultra-fast cPCR run time. For singleplex ultra-fast cPCR, the primer concentration needed to be lowered to 3 μM or the run time needed to be reduced to 14 minutes. For duplex ultra-fast cPCR, 2 μM of each primer set needed to be used or the run time needed to be reduced to 14 minutes. Under the conditions optimized in this study, the combination of ultra-fast cPCR and NALF immunoassay can be applied to on-site detection of pathogens. The combination can be easily applied to the detection of oral pathogens.

The vehicle routing problem is one of the vibrant research problems for half a century. Many studies have extensively studied the vehicle routing problem in order to deal with practical decision-making issues in logistics. However, developments of new logistics strategies have inevitably required investigations on solution methods for solving the problem because of computational complexity and inherent constraints in the problem. For this reason, this paper suggests a simulated annealing (SA) algorithm for a variant of vehicle routing problem introduced by a previous study. The vehicle routing problem is a multi-depot and multi-trip vehicle routing problem with multiple heterogeneous vehicles restricted by the maximum permitted weight and the number of compartments. The SA algorithm generates an initial solution through a greedy-type algorithm and improves it using an enhanced SA procedure with three local search methods. A series of computational experiments are performed to evaluate the performance of the heuristic and several managerial findings are further discussed through scenario analyses. Experiment results show that the proposed SA algorithm can obtain good solutions within a reasonable computation time and scenario analyses show that a transportation system visiting non-dedicated factories shows better performance in truck management in terms of the numbers of vehicles used and trips for serving customer orders than another system visiting only dedicated factories.

This study was conducted to determine the applicability of the shoot growth model to cut roses grown at high temperatures. Two cultivars of cut roses, ‘Antique Curl’ and ‘Beast’, were cultivated in growth chambers set to night/day temperatures of 24/20°C for the control group and 32/28°C for the treatment group. The shoot growth model, which resulted in high coefficients of determination (R2 = 0.80 and 0.62, respectively, for ‘Antique Curl’ and ‘Beast’), was a good predictor of the decrease in the growth of rose shoots for both treatment groups. Although the model produced good results for both the control and treatment groups of ‘Antique Curl’, the accuracy of the model can be improved by using the modified leaf area at the harvest stage. From these results, the model was confirmed to perform well for predicting a decrease in productiveness and quality in cut roses cultivated during summer under high temperatures.

Inflammation is an important protective response mechanism that occurs against microbial invasion or injury. However, excessive inflammation may lead to cause of morbidity and mortality in diseases. The activated macrophages plays a vital role in inflammatory response by stimulation of lipopolysaccharide (LPS) and tumor necrosis factor-α (TNF-α). This activation further damages the host by inducing certain pro-inflammatory mediators such as nitric oxide (NO), interleukin-1β (IL-1β), interleukin- 6 (IL-6), TNF-α, inducible nitrous oxide (iNOS) and cyclooxygenase (COX-2). Flavonoids are bioactive compounds with potential effects as anti-cancer, anti-inflammation, anti-viral and anti-bacterial activities. Polymethoxyflavones (PMFs) are unique to citrus plants which are of specific interest owing to their biological effects that includes lipoprotein metabolism and anti-inflammatory activity. Sinensetin is one of the PMFs having five methoxy groups on the basic benzo-γ-pyrone skeleton with a carbonyl group at the C4 position. Sinensetin have been known for exerting various pharmacological activities including anti-angiogenesis, anti-diabetic and anti-inflammatory activities. However, there are no studies focused on the anti-inflammatory effects of sinensetin on skeletal muscle cells. In the present study, we investigated the antiinflammatory effect of flavonoids isolated from Sinensetin on the production of pro-inflammatory mediators mediated by nuclear factor-kappa B (NF-κB) by inhibition of signal transduction in LPS - induced L6 skeletal muscle cells.

This study investigated the effects of negotiation in the process of providing written corrective feedback (WCF) by comparing three different patterns: non-negotiation, minimal negotiation, and extended negotiation. The data were collected from three intermediate academic English classes at a university in Korea. Each class was exposed to different degrees of negotiation while they received WCF for two target forms, the past verb forms and the English articles, in their written production (text re-construction tasks). Their accuracy improvement was measured by text-reconstruction writing tasks and error correction tests. The study found that the use of negotiation affected accuracy improvement of the target forms; however, its effectiveness varied depending on the target forms and measurements. For the past tense, negotiation, regardless of its amount, resulted in more accuracy improvement than non-negotiation in both text re-construction tasks and error correction tests. However, for the English articles, negotiation operated differently: extended negotiation significantly brought about more accuracy development in both measurements, but the difference between minimal negotiation and non-negotiation was only observed in the error correction test, not in the text re-construction task.

In this study, we examined the protective immunity of a combination of seven Brucella abortus recombinant proteins; superoxide dismutase (rSodC), riboflavin synthase subunit beta (rRibH), 50S ribosomal protein (50s rL7/L12), nucleoside diphosphate kinase (rNdk), malate dehydrogenase (rMDH), arginase (rRocF), and elongation factor (rTsf) cloned in a pMal vector system and expressed in DH5α. Mice groups were immunized thrice with a combined subunit vaccine (CSV-7) at 0, 2, and 5 weeks and subsequently challenged with B. abortus at 5 × 104 CFU at 6 weeks. At four weeks post-infection, the mice were sacrificed and the bacterial burden in their spleens was quantified. Results revealed bacterial log reductions of 0.63 and 0.34 in comparison to PBS and maltose-binding protein (MBP), respectively. Cytokine profiling revealed a marked increase in IFN-γ (interferon-gamma), MCP-1 (macrophage chemoattractant protein-1) and IL-6 (interleukin 6) cytokines at 5-weeks post-immunization. On the other hand, only TNF was heightened at 7-weeks post-immunization. In general, this cytokine profile is consistently reflective of a Th1 immune response, which is beneficial for host immunoresistance.

In March of 2015, severe sooty mold was observed on blueberries cultivated in a farmer’s greenhouses in Goseong-gun, Gyeongsangnam-do, South Korea. Typical symptoms included a black or dark brown, superficial fungal growth on the leaves, stems, and fruit of blueberry plants. The fungal colonies on PDA were flat and black. The conidia were dark green in color, globose to sub-globose in shape and 3-5×3-4 μm in size. The ramoconidia were cylindrical and 18-42×3 μm in size. The conidiophores were dark brown, cylindrical-oblong in shape and 42-138×3-4 μm in size. To confirm the identity of the fungus(F130), the 18S rDNA as well as actin and β-tubulin gene sequences were PCR-amplified and sequenced. The pathogenicity of the isolate was then explored on 2-month-old potted blueberry plants. Based on the mycological characteristics, molecular identification, and pathogenicity on host plants, this fungus was identified as Cladosporium sphaerospermum. This is the first report on sooty mold of blueberry caused by C. sphaerospermum in Korea.

원심 해수냉각 펌프를 분석하기 위하여 다른 운전 유량에 대한 캐비테이션 거동을 조사하였다. 3D 2상 해석은 ANSYS-CFX 상 용코드로 수행되었다. 해석에는 k-ε 난류와 Rayleigh-Plesset cavitation 모델이 사용되었다. 수치 예측에 기초하여 세 가지 토출 유량값에 대 하여 헤드 드롭 특성곡선이 작성되었다. 더 높은 유량에서 임펠러는 버블 캐비테이션에 보다 취약하다. 0.7Q, Q 및 1.3Q(Q: 설계 유량)에서 작동하는 펌프의 3 % 헤드 드롭 위치는 각각 NPSHa 1.21 m, 1.83 m 및 3.45 m에 해당한다. 증기 기포의 볼륨이 예측되고 캐비테이션의 위치는 임펠러 내에서 발생하는 캐비티를 시각화하여 예상하였다. 또한, 압력계수와 날개 부하 분포가 구체적으로 제시되어 캐비테이션이 펌프 운전에 미치는 해로운 영향을 나타냈다. 또한, 압력계수 분포와 날개부하 차트가 구체적으로 제시되어, 펌프 운전에 캐비테이션이 미치는 해로운 영향을 나타냈다.

By analyzing the status of Korean tire labeling regulation and comparing the performance indicators of tire labeling in different countries, this paper expounds the serious impact of tire noise on traffic noise pollution, and puts forward that in order to improve the comfort of car ride, control traffic noise pollution and build a green tire industry, the tire labeling system should be improved, and the corresponding requirements for tire rolling noise should be put forward according to the road conditions in Korea.

This study was aimed to determine the effects of hybrid and inoculant on in vitro rumen digestibility and rumen fermentation indices of corn silage. Two corn hybrids(Kwangpyeongok (KW) vs. Pioneer 1543(PI)) were ensiled for 100 days with two inoculants(L. plantarum(LP) vs. L. buchneri(LB)) applied at 1.2 × 105 cfu/g of fresh forage. Silages were incubated in rumen fluid medium at 39℃ for 72 hours. Both of KW and PI had no difference(p>0.05) on in vitro digestibility of dry matter(IVDMD) and neutral detergent fiber(IVNDFD), while application of LB produced higher(p<0.05) IVDMD and IVNDFD than LP. Hybrid and inoculant applications did not affect(p>0.05) total gas volume, ammonia-N, total VFA, and acetate concentrations in the rumen. Applied LB was higher(p<0.05) propionate concentration than LP, while hybrids had no effect(p>0.05). Furthermore, the interaction effect(p<0.05) was occurred in propionate concentration. Butyrate concentration did not affected(p>0.05) by hybrid and inoculant applications, but there was an interaction effect between hybrid and inoculant(p<0.05). Applications of hybrid and inoculant had effects(p<0.05) on iso-butyrate and valerate, without any interaction effects(p>0.05). This study concluded that application of KW and PI has no effects on in vitro rumen digestibility and rumen fermentation indices of corn silage, while application of LB increase the rumen digestibility in both of corn hybrids.

The study on the tire noise due to the On-Board Sound Intensity test method. The object of this project is to prepare the countermeasure of the noise reduction according to collect tire/road noise data under various influencing factors by using On-Board Sound Intensity test method. To accomplish the object ; A automotive is used as a test platform to test tire/road noise by changing different types of tires and using On-Board Sound Intensity test system. The influence of inflation pressure, road, load, speed and pattern type on tire/road noise is compared and analyzed. At the same time, the mechanism of tire/road noise is analyzed, which provides a experimental basis for tire noise reduction.

We have studied a method to prepare polydopamine-modified reduced graphene oxide-supported Pt nanoparticles (Pt– PDA–RGO). The Pt–PDA–RGO nanocomposites were synthesized by a wet-coating process, which was induced by selfpolymerization of dopamine. As an eco-friendly and versatile adhesive source in nature, dopamine could be easily adhered to surfaces of organic material and inorganic material via polymerization processes and spontaneous adsorption under weak alkaline pH conditions. To apply the unique features of dopamine, we synthesized Pt–PDA–RGO nanocomposites with a different quantity of dopamine, which are expected to preserve the improved Pt adsorption on graphene, resulting in the enhanced electrocatalytic performance. The morphology and micro-structure were examined by field emission scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. Compared to pristine Pt–deposited RGO (Pt–RGO), Pt–PDA–RGO (30 wt% dopamine against RGO) nanocomposites showed a superior electrochemical active surface area for a methanol oxidation. This could be related to the fact that the optimized c

북베트남의 컨테이너 터미널을 주제로 한 연구는 다수 있지만, 선박의 대기시간이나 화물처리에 관련한 비용 측면에서의 경쟁을 다룬 연구는 거의 없다. 이 논문은 북 베트남에 입지한 컨테이너 터미널의 TEU당 평균대기비용을 추산해 본 연구다. 우선 대기 행렬이론을 적용하여 평균대기시간을 추산한 뒤, 불확실성 이론을 적용하여 선박의 일당 비용을 추산할 것이다. 그리고 각 터미널의 하역능력 내지 물동량 처리율과 관련하여 TEU당 일련의 대기시간을 산정하기 위해 시뮬레이션을 실시하였다. 추산된 일련의 대기시간을 근거로 하여 각 항만의 평균대기시간과 물동량 처리율 / 하역률 간의 관계를 제시하기 위한 함수를 추정하기 위해 비선형회귀법을 적용하였다. 연구 결과는 북베트남의 컨테이너 터미널간의 경쟁상황에 관한 후속 연구에서 게임이론을 적용하는 데 이용될 수 있을 것 이다.

In this study, we studied the alignment characteristics of liquid crystal on polyimide substrate when irradiated with ion beam in oblique direction on uniformly coated polyimide substrate. The inclined irradiation angle of the ion beam was set to 30 degree, 45 degree and 60 degree and the characteristics were observed for 1 minute and 2 minutes at an ion beam irradiation intensity of 600 to 3,000 eV for each angle. The Alignment of the liquid crystals were observed using a polarized microscope after preparing antiparallel specimens. The pretilt angle of the liquid crystal using the crystal rotation method was measured, and the applicability to actual products was evaluated. Experimental results showed that uniformly aligned liquid crystals could be obtained in samples irradiated at 1 and 2 minutes with an ion beam intensity of 1200 eV or higher when irradiated with 30 degree and 45 degree tilted ion beams. And, at 60 tilted degree, It shows that uniformly aligned liquid crystals could be obtained in samples irradiated at 1 and 2 minutes with an ion beam intensity of 2400 eV or higher. The pretilt angle of the liquid crystal showed the best characteristics when irradiated with ion beam inclined at 45 degrees, and it was confirmed that the pretilt angle was 0.2 to 1.3 degrees, which is usable for horizontally oriented LCD.

We designed a system that can automatically collect, convey, and control cool air of 15oC-20oC containing carbon dioxide from a mushroom cultivation house to a strawberry plastic house. We recorded the temperature at various positions from July to August 2017. The average temperature of the green house during day and at night was maintained at 33oC and 26oC, respectively. In the moveable three-tier cylindrical bed, the average temperature around root was maintained at 26oC and 21oC during day and at night, respectively. On the high-bench in the green house, the temperature was maintained at 32oC and 30oC during day and at night, respectively. The carbon dioxide concentration was maintained around 800-1,600 ppm in the mushroom cultivation system and 400-800 ppm in the strawberry plastic house. The growth characteristics of the strawberry treated with moveable three-tier cylindrical bed were significantly different from those of the untreated high-bench bed. In addition, during the summer season, moveable three-tier cylindrical bed showed more tendency to increase in normal fruit number (NFN) and to decrease in defective fruit number (DFN) compare to the high-bench bed. Therefore, the moveable three-tier cylindrical bed showed a tendency to be more than 2 times higher yields than that of the high-bench bed. It was confirmed that everbearing strawberry cultivars could be cultivated in green house due to the cool air supply from the mushroom cultivation system in the summer season.