Intermuscular fat is essential for enhancing the flavor and texture of cultured meat. Mesenchymal stem cells derived from intermuscular adipose tissues are a source of intermuscular fat. Therefore, as a step towards developing a platform to derive intermuscular fat from mesenchymal stem cells (MSCs) for insertion between myofibrils in cultured beef, an advanced protocol of intermuscular adipose tissue dissociation effective to the isolation of MSCs from intermuscular adipose tissues was developed in cattle. To accomplish this, physical steps were added to the enzymatic dissociation of intermuscular adipose tissues, and the MSCs were established from primary cells dissociated with physical step-free and step-added enzymatic dissociation protocols. The application of a physical step (intensive shaking up) at 5 minutes intervals during enzymatic dissociation resulted in the greatest number of primary cells derived from intermuscular adipose tissues, showed effective formation of colony forming units-fibroblasts (CFU-Fs) from the retrieved primary cells, and generated MSCs with no increase in doubling time. Thus, this protocol will contribute to the stable supply of good quality adipose-derived mesenchymal stem cells (ADMSCs) as a fat source for the production of marbled cultured beef.

A facile and efficient method was developed to prepare highly stretchable and conductive graphene conductors with wrinkled structures by the mechanical stretching and shrinking of elastomeric substrates, in which graphene inks were printed on a prestretched elastomeric substrate. Stretchable and exfoliated graphene inks were prepared by mixing graphite and Ecoflex in a shear-assisted fluid dynamics reactor. The resultant graphene conductor exhibited excellent stretchability at 150% strain and high electrical conductivity of 64 ± 1.2 S m− 1. The resistance of the conductor did not change in bent, twisted, and stretched states. The resistance did not change during 10,000 cycles of stretching/releasing, with a maximum strain of 150%. Based on the graphene conductor, a stretchable conductometric sensor with a two-electrode configuration was fabricated to measure impedance changes at different concentrations of electrolyte ions. This sensor exhibited a good and linear sensitivity curve (298.61 Ω mM− 1, R2 = 0.999) in bent and stretched states.

본 연구는 관상가치가 있는 자생 참두메부추와 갯부추를 절화소재로 이용하고자 수행되었다. 실험은 절화가 수확된 직후, gibberellic acid(GA3) 50, 75, 100mg·L-1, silver thiosulfate(STS) 0.1, 0.3, 0.5mM, 8-hydroxyquinoline sulfate(8-HQS) 25, 50, 100mg·L-1, 그리고 시중에 판매되 고 있는 Chrysal 8mL·L-1, Floralife 10mL·L-1의 보존용액 에 처리되었다. 참두메부추의 절화수명 연장에는 Chrysal 보 존용액 처리가 가장 효과적이었으며 다음으로 75mg·L-1 GA3 처리가 효과적이었다. 한편 8-HQS와 STS는 참두메부추의 절화수명을 단축하고 줄기가 갈변하는 등의 부정적인 영향을 끼쳤다. Chrysal과 더불어 Floralife 보존용액 처리는 절화 참두메부추의 상대 생체중변화율을 높이는데 효과가 있었다. 반면 갯부추는 100mg·L-1 GA3 처리에서 절화수명이 유일하 게 7일까지 연장되었다. GA3 보존용액 처리를 제외한 다른 처리에서 갯부추의 절화수명은 증류수인 대조구보다 비슷하 거나 약간 높은 수준이었다. 절화 갯부추의 수분흡수율은 실 험 초반 100mg·L-1 8-HQS 처리에서, 상대 생체중변화율은 Chrysal 보존용액 처리에서 가장 높게 조사되었으나 두 처 리 모두 절화수명이 유의하게 연장되지 않았다. 갯부추는 100mg·L-1 GA3 처리에서 절화수명을 비롯한 절화품질이 가 장 우수하였다.

L-asparaginase (ASNase) is a therapeutic enzyme used to treat acute lymphoblastic leukemia. Currently, the most widely used ASNases are originated from bacteria. However, owing to the adverse effects of bacterial ASNases, new resources for ASNase production should be explored. Fungal enzymes are considered efficient and compatible resources of natural products for diverse applications. In particular, fungal species belonging to the genus Trichoderma are well-known producers of several commercial enzymes including cellulase, chitinase, and xylanase. However, enzyme production by marine-derived Trichoderma spp. remains to be elucidated. While screening for extracellular ASNase-producing fungi from marine environments, we found four strains showing extracellular ASNase activity. Based on the morphological and phylogenetic analyses using sequences of translation elongation factor 1-alpha (tef1α), the Trichoderma isolates were identified as T. afroharzianum, T. asperellem, T. citrinoviride, and Trichoderma sp. 1. All four strains showed different ASNase activities depending on the carbon sources. T. asperellem MABIK FU00000795 showed the highest ASNase value with lactose as a carbon source. Based on our findings, we propose that marine-derived Trichoderma spp. are potential candidates for novel ASNase production.

Image recognition is not very effective in the water environment due to multiple factors, such as high scattering and high scattering in the water column. This is why the relevant parameters in the Faster R-CNN network model need to adjust continuously to improve the effectiveness of water detection. The control variable method adjusts the program's learning rate by tuning the network model's parameters. Then, the number of training rounds is adjusted according to the loss function of each round, and finally, we can get the number of matches with the minimum loss function. Based on the experimental results on the dataset, it is shown that the proposed method not only selects the learning rate with the best detection results but also has the strongest robustness and achieves a 96%-99% recognition rate for passenger ships, cargo ships, warships, and bridges compared with other learning rates. Experiments show that the Faster R-CNN network model detects water targets with significant results, and the best network model learning rate parameter is 6×10-3.

Repetitive or excessive exposure to ultraviolet (UV) radiation causes oxidative stress-mediated skin photoaging through the overproduction of reactive oxygen species. Actinidia polygama is known as a medical plant used in oriental medicine for treating several diseases such as abdominal pain, stroke and rheumatoid arthritis. Recently, it was reported that A. polygama extract had anti-wrinkle and skin hydrating properties in ultraviolet B (UVB)-exposed hairless mice. However, the molecular biological mechanism of this extract on alleviating skin photoaging is still unknown. Therefore, we investigated the anti-photoaging effects of PB203, which is the powder of A. polygama extract, in the in vivo and in vitro photoaging models. First, PB203 showed 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities due to the presence of anti-oxidant components including flavonoids and polyphenols. In UVB-irradiated hairless mice, oral administration of PB203 (100 mg/ kg) significantly improved wrinkle formation, skin dehydration, elasticity and skin barrier function by decreasing the levels of matrix metalloproteinases (MMPs) and increasing those of collagen I, filaggrin, involucrin and loricrin. Especially, the reduced production of p-p38, p-c-Jun and p-c-Fos by PB203 reversed the elevated levels of MMPs mediated by UVB exposure, resulting in the upregulation of collagen I expression. Consistent with these animal data, PB203 remarkably enhanced the mRNA expression of collagen I, filaggrin, involucrin and loricrin, while suppressed that of MMPs in UVB-irradiated HaCaT cells. And PB203 increased the wound recovery rate of cells by promoting their proliferation and migration. Moreover, PB203 significantly recovered the activity of superoxide dismutase inhibited by UVB in both mice and cells. In conclusion, PB203, which protects skin from UVB-induced photodamage by exerting antioxidant properties, can be considered to have sufficient potential as a functional ingredient or therapeutic agent improving skin photoaging and related skin symptoms.

Many manufacturers applying third party logistics (3PLs) have some challenges to increase their logistics efficiency. This study introduces an effort to estimate the weight of the delivery trucks provided by 3PL providers, which allows the manufacturer to package and load products in trailers in advance to reduce delivery time. The accuracy of the weigh estimation is more important due to the total weight regulation. This study uses not only the data from the company but also many general prediction variables such as weather, oil prices and population of destinations. In addition, operational statistics variables are developed to indicate the availabilities of the trucks in a specific weight category for each 3PL provider. The prediction model using XGBoost regressor and permutation feature importance method provides highly acceptable performance with MAPE of 2.785% and shows the effectiveness of the developed operational statistics variables.

본 논문에서는 외계 행성에서 주어진 임무를 모두 수행하여 클리어하는 탑 뷰 슈팅 게임을 제안한다. 제안 하는 게임을 진행한 플레이어 45명의 로그 기록과 설문 조사 결과를 분석하여, 몰입감, 플레이 패턴의 다양 성, 접근성을 중심으로 제안하는 게임의 특징을 살펴보고자 한다. 첫째, 제안한 게임은 더 높은 몰입감을 위 해 스토리 애니메이션과 오디오를 제공한다. 실험 결과 시각, 청각적 방면으로 몰입감을 효과적으로 제공하 고 있어 대다수의 플레이어가 스토리 애니메이션을 건너뛰지 않고 끝까지 시청했다. 둘째, 제안하는 게임은 더 다양한 플레이 패턴으로 즐길 수 있다. 실험 결과 수많은 경우의 수가 존재하는 무기와 스킬을 선택할 수 있게 하였기 때문에 대부분의 플레이어가 선택하는 스킬들이 서로 겹치지 않고 다양한 방식으로 게임을 플레이할 수 있다. 플레이 패턴이 다양한 만큼 더 게임을 오랫동안, 더 재미있게 즐길 수 있다. 셋째, 제안한 게임은 맵을 비교적 단순하게 구성하였다. 그런데, 호기심 많은 일부 플레이어들이 미션과 직접 관련이 없는 경로를 찾아서 길을 헤매는 경우가 나타났다. 이로 인해, 플레이어들이 게임을 다시 한번 진행하고자 하는 경향이 나타났다.

디지털로 시작된 세대 간의 지식전복과 이에 따른 격차는 게임이라는 콘텐츠를 통해 그 차이점을 드러낼 것이라는 것을 가정하고 본 연구를 수행하였다. 이를 증명하기 위해 실제 부모의 게임에 관한 인식이 자녀 의 삶에 중요한 변수로 자리를 잡고 있는지 분석해 보는 것이 본 연구를 통해 알아보고자 하는 것이 주요 내용이었다. 분석 결과, 부모의 게임인식은 자녀의 삶에 직접적으로 영향을 미치진 않았다. 하지만 자녀가 판단했을 때 부모의 게임인식 수준은 자녀의 삶의 만족도에 상당한 영향을 미치고 있는 것으로 확인이 되 었다. 이는 부모가 스스로 판단하는 게임인식보다, 디지털 세대인 자녀의 게임 활동과 문화를 어떻게 인식 하고 대하는지에 따라 자녀의 행복에 영향을 미친다는 것이다. 본 연구의 결과는 자녀가 인식하는 부모의 게임인식과 자녀의 게임인식 간의 격차는 청소년 자녀의 행복에 매우 중요한 요인으로 작용을 하며, 이는 청소년 자녀의 게임 활동을 부모세대가 꼭 배우고 습득하는 것만이 자녀 세대를 이해하는 길이 아니라, 자 녀와 함께 소통하면서 현재의 상황을 이해하는 것이 보다 중요한 요인으로 나타났다고 할 수 있다. 시대적 상황에 의하여 한 가정 내에 부득이하게 발생한 문화적 취향의 차이지만, 이를 부모가 인정하고 이해하는 방향으로 소통을 진행한다면 자녀의 삶에 매우 긍정적인 영향을 줄 수 있다는 것을 의미하기도 한다.