극심한 고온으로 인해 정상적인 작물의 재배가 어려운 여름철의 온실 내 기온을 억제시키기 위한 방법 중 가장 효율을 인정받고 있는 증발냉각법은 실내 습도의 증가로 인해 그 사용에 제한을 받게 된다. 본 연구에서는 온실에서 증발냉각법의 냉방효율을 높이기 위한 방법으로 냉수파이프를 설치하여, 그 열적 특성을 분석하고, 제습효과를 조사하였으며, 그 결과를 요약하면 다음과 같다. 동파이프와 아연도금스틸파이프의 총열전달량을 비교한 결과, 동파이프가 다소 크게 나타났으나, 유의성이 없었으며, 두 가지 파이프 모두 이론값 보다 실측값이 더 작게 나타났다. 냉수파이프 표면에 응축되어 제거된 수증기의 양은 동파이프와 아연도금스틸파이프 사이에서 큰 차이를 보이지 않았으나, 두 가지 파이프 모두에서 제습효과는 충분히 큰 것으로 나타났다. 냉수파이프를 설치함으로써 증발냉각시스템의 냉방효율을 평균 48%만큼 높일수 있으며, 평균 1.3℃만큼의 실내기온을 추가로 냉각시킬 수 있는 것으로 예측되었다. 또한, 냉수파이프를 이용해 증발냉각시스템의 가동으로 인한 실내의 과습문제를 해결하기 위해서는 파이프표면의 응축된 수분을 효과적으로 제거할 수 있는 장치나 방법에 대한 추가적인 연구가 필요할 것으로 판단된다.

Polyoxyethylene monooleate was prepared by addition of ethylene oxide to oleic acid. And also, polyoxyethylene monooleate type oil dispersant was prepared by blending polyoxyethylene monooleate, n-paraffine, sorbitan monooleate, sorbitan monopalmitate, and palm oil. Dispersion efficiency test was carried out by vertical shaking flask and swirling flask methods. Low toxic oil dispersant was prepared with polyoxyethylene monooleate, which has high biodegradability and excellent dispersion efficiency on crude oils and weathered W/O emulsions with high viscosity, and its dispersion efficiency was measured to various crude oils and weathered oils.

This experiment was carried out to investigate the factors affecting superovulation in rabbits and to determine the effect of pFSH and PMSG on ovarian superovulatory responses and embryo production, and the effect of superovulation treatment with a single injection of pFSH dissolved in polyvinylpyrrolidone on the ovarian responses and the embryo quality. The results obtained were suonmerized as follows: Superovulatory response resulted in significantly (P<0.05) higher ovulation rates and more embryos in spring or autumn, compared with summer or winter. Repeated superovulatory treatments with PMSG leaded to a significantly(P<0.05) decreased number of total follicles and recovered ova. Superovulation with pFSH resulted in the higher number of ovulated follicles and recovered ova than with PMSG. A single subcutaneous injection of pFSH dissolved in 25% PVP resulted in the more ovulation points(33.2) and recovered embryos(30.2), which were comparable to the multiple injections of pFSH(44.8 vs 37.7).These results indicated that the treatment with a single injection of FSH dissolved in PVP was an efficient and simple alternative method to the conventional multiple FSH injections for superovulation in rabbits.

본 논문은 바닥판을 고려한 3차원 골조 구조물의 효율적인 연직 진동 해석법을 제안한다. 바닥판의 영향을 고려하면 구조물의 진동해석을 좀더 정확히 수행할 수 있는 반면, 면요소의 사용에 따른 많은 자유도의 사용으로 많은 해석시간과 기억용량이 필요하게 된다. 따라서, 행렬응축기법을 사용하여 적절한 요소분할 및 주자유도 선택 기법을 제안하였으며, 대형요소를 사용하여 정확화된 일반적인 건물의 동적해석에 적용하여 입력 데이터의 작성을 간단히 하고 동적해석을 빠르고 신뢰할 만한 결과를 얻을 수 있는 기법에 대하여 연구하였다.

This study was carried out to establish an effective system for embryo transfer techniques by analyzing several factors affecting in-vivo embryo transfer in Korean cattle Embryos were transferred into a toral of 301 recipients. The results obtained in studies on the factors affecting pregnancy rate after embryo transfer by condition of transfer time were as follows ; 1. The pregnancy rate by the seasons of transferred fresh and frozen embryos were not different, but the pregnancy rate was slightly higher in summer(80.8%). 2. The pregnancy rate by the days of embryo transfer after estrus were not different when fresh embryos were transferred, but the pregnancy rate was highest at 8 days when frozen embryos were transferred(P<0.01, 40.0%). 3. The pregnancy rate at estrus synchronization was remarkably higher with PGF treated than natural (P<0.05, 70.4%, 43.4%). 4. The pragnancy rate by the degree of estrus synchronization was best when the estrus was synchronized in both fresh and frozen embryos (83.3% and 29.7%, respectively), but the pregnancy rate was not different among 2 days. But the pregnancy rate of frozen embryos were slightly higher when the recipients exhibited estrus earlier than donors.

This study was carried out to establish an effective system for embryo transfer techniques by analyzing several factors affecting in-vivo embryo transfer in Korean cattle. Embryos produced in-vivo were transferred into a total of 301 recipients The results obtained in studies on the factors affacting pregnancy rate after embryo transfer by condition of recipients were as follows ; 1. The pregnancy rate by age and parity of recipients showed high in 5~8 and over 12 years old(72.7~73.9%), and 3rd~4th parity(82.1%) for fresh embryos(P<0.05). The pregnancy rate did not differ by age and parity of recipients in frozen embryos. The pregnancy rate of frozen embryos tended to be similar to that of fresh embryos(38.5% and 25.0~36.7%). 2. The number of observation for normal estrus cycles of recipients did not differ In pregnancy rate between one and 2 times in fresh embryos(64.9%, 69.8%). The pregnancy rate by transferred frozen embryos showed significantly higher after 2 times of observation(P<0.05, 16.3%, 37.5%). The pregnancy rate by days open did not differ between fresh and frozen embryos. But the pregnancy rate was slightly higher in 12 months and 6 months of days open for fresh and frozen embryos, respectively(70.1~71.1% and 24.5%, respectively). 3. The pregnancy rate of transferred fresh and frozen embryos into right and left side of uterine horn did not differ(62.1% : 65.9% 25.0% : 24.3%, respectively). The pregnancy rate by the grade of CL was not different in fresh embryos, but the pregnancy rate was significantly higher in the grade A than B for frozen embryos(P<0.01, 43.2%, 16.2%).

This study was carried out to establish an effective system for embryo transfer techniques by analyzing several factors affecting in-vivo embryo transfer in Korean cattle. Embryos produced in-vivo were transferred into a total of 301 recipients. The results obtained in studies on the factors affecting pregnancy rate after embryo transfer by condition of embryos were as follow ; 1. The pregnancy rate of 301 recipients was 45.2% and higher with fresh embryos than with frozen embryos(63.5% : 21.4%, P<0.01). Embryos superovurated by FSH-P had slightly greater than by SUPER-OV in pragnancy rate, athough these were no difference between two treatments. 2. The pregnancy rates of transferred morulae and blastocysts showed no difference between fresh and frozen embryos(63.5% : 63~6% ; 20.0% : 25.8%). However, the pregnancy rates by quality of flesh and frozen embryos were significantly different(P<0~05). The pregnancy rates were outstandingly high in the grade A, B of fresh embryos(59.0~66.4%), and in the grade A of frozen embryos(43.6%). 3. The number of transferred embryos showed no difference in pregnancy rate, but when frozen embryos transferred, the pregnancy rate was slightly higher with two embryos than that with one embryo.

This study was to determine the effect of ionomycin and 6-dimethylaminopurine (6-DMAP) and/or elcetrical stimulation on the oocyte activation and production of rabbit nuclear transplant embryos. The oocytes were collected from the oviduct of superovulated rabbits at 14 h post hCG injection and cultured in TCM-199 containing 10% FBS until 19 h post hCG injection. To determine the optimum concentration and exposure time of 6-DMAP, some oocytes were activated with 5 M ionomycin for 5 min and then in 2.0 mM 6-DMAP for 0.5 to 3.0 h, or in 1.0 to 3.0 mM 6-DMAP for 2.0 h. Other control oocytes were stimulated electrically(3X, 1.25 kV/cm, 60 sec) in 0.3 M mannitol solution supplemented with 100 M CaCl and MgCl. The nuclear donor embryos of 8-cell stage were synchronized to G phase of 16-cell stage, and the recipient cytoplasms were obtained from removal of the first polar body and a portion of membrane bound cytoplasm of the oocytes collected at 15 h post hCG injection. A separated blastomere was injected into the perivitelline space of the enucleated oocytes. The oocytes injected with nucleus were cultured until 19 h post hCG and then electrofused and activated by electrical stimulation with or without ionomycin and 6-DMAP. These nuclear transplant embryos were cultured in TCM-199 containing 10% FBS in 39˚C, 5% CO2 incubator for 120 h. For the oncytes activated parthenogenetically with electrical stimulation with or with-out ionomycin and the various concentration of exposure time of 6-DMAP, the highest cleavage(92.3%) and development to blastocyst stage(41.0%) were resulted from the oocytes activated by ionomycin and 2.0 mM 6-DMAP for 2.0 h, which were found to be significantly(P<0.05) higher than the cleavage(45.2%) and developement to blastocyst stage(14.3%) from the oocytes activated with electrical stimulation. The significantly(P<0.05) more oocytes(71.4%) developed to 4 cell stage at 24 h post activation by ionomycin and 6-DMAP than those by electrical stimulation(18.9%). For the nuclear transplant embryos, the cleavage rate was similarly high in oocyte activation by electrical stimulation with(79.4%) or without ionomycin and 6-DMAP(70.5%). However, the embryo development to blastocyst stage was significantly(P<0.05) higher in oocyte activation by electrical stimulation with ionomycin and 6-DMAP(44.4%) than by electrical stimulation only(25.0%). The significantly(P<0.05) more nuclear transplant embryos(45.6%) developed to 4 cell stage at 18 h post activation by electrical stimulation with ionomycin and 6-DMAP than those by electrical stimulation only(10.6%). These results indicated that the supplemental oocyte activation by ionomycin and 6-DMAP with electrical stimulation enhanced and accelerated the preimplanted in vitro development of the rabbit nuclear transplant embryos.

겨울철 오이 시설재배에서 태양열 시스템을 이용한 지중가온의 효과를 구명하고자 지중 40 cm에 15 mm의 PPC파이프를 130 cm이랑 에 4열 매설한 후 지중 20 cm의 지온을 22~23℃로 설정한 후 1996년 11월 7일부터 1997년 1월 30일까지 일정한 온도로 유지, 관리하여 무가온구와 가온구의 지상부, 지하부 및 수량을 비교시험한 결과는 다음과 같다 1) 지중가온에 의한 지온확보는 가온구는 15~20 cm에서 22℃ 정도의 평균온도를 확보할 수 있었고, 무가온구는 평균 17~18℃ 정도였다 2) 정식 30일 정도에서 초기생육은 가온구가 초장, 경경, 엽수, 엽면적 등 모두 증가하였으며 특히 초장 27%, 엽수 51%, 엽면적은 150% 정도 증가하였다. 또 지상부 평균 증가율도 관행대비 가온구 증가율이 117% 정도였다. 3) 가온구의 지하부 뿌리의 생장성이 관행구에 비하여 평균 56% 정도 증가하였다 4) 과수의 수량면에서도 총과수가 무가온구 313개, 가온구 614개로 가온구가 196% 정도 증수되었다.

태양열 에너지의 효율적인 이용과 자동화 장치의 개발을 목표로 지중가온의 온도변화 특성을 실험. 분석한 결과는 다음과 같다. 1) 10월 13일의 1일 하우스 내기온이 주야간에 24℃의 차이가 있으며, 무가온시 지온변화는 지중 10 m 부근에서 6℃, 지중 20 cm 부근에서는 3℃정도의 차이를 보이고 있다. 2) 20시경에 내기온과 지온차가 가장 작은 것으로 나타났으며, 지중 20 cm 부근의 온도변화는 내기온이 가장 낮은 오전 7시부터 약 3시간이 경과된 오전 10시에 최저가 되었다 3) 가온수의 온도를 40℃, 50℃, 60℃로 변화하였을 때 지중 10 cm의 최저은도는 약 20℃ 지중 20 cm의 최저온도는 약 23℃로 나타나 가온온도가 40℃ 이상일 경우 가온온도에 따른 지중 10~20 cm사이의 온도차는 매우 작았다. 4) 지중 15~20 cm의 지온이 20℃가 되기 위해서는 가온수의 온도를 40℃ 이하가 되도록 설정하여야한다. 5) 가온수의 온도가 40℃, 50℃, 60℃이고 파이프 매설 깊이가 12 m일 경우 유입구와 유출구의 1일 평균온도차는 40℃일 경우 3.5℃ 50℃일 경우 4.4℃, 60℃일 경우 5.4℃정도로 이 구간에서 온도변화식은 T = 0.09591T＋2.5451(R2= 0.9966)로 거의 선형적으로 변화하였다. 6) 가온수 온도가 40℃의 경우 지중 15~20 cm, 50℃의 경우는 지중 13~19 cm, 60℃의 경우는 12~17 cm 부근이 경계영역으로 판단되었다. 7) 재배기간중 하우스 내기온을 11℃ 이상으로 유지하고, 가온수의 온도를 28℃로 순환 결과 지중 15 cm 이하에서 최저지온를 20℃ 이상의 온도를 유지할 수 있어 저온수공급에 의한 온도상승효과가 뚜렷이 나타났다. 8) 가온수의 온도를 28℃로 하여 지중가온 한 결과 지중 15~20 cm사이에 온도변화는 무가온구에 비하여 공히 4℃~7℃가 상승되었다.