This study investigated the physical properties of polymers and antimicrobial activities of organic acids on Listeria monocytogenes to develop hydrogels. κ-carrageenan (1, 2, and 3%), carboxymethylcellulose (CMC; 1, 3, and 5%), and agar (1.5 and 3%) were mixed with cross-linkers (Na+, K+, Ca2+, and Al3+) or each other by stirring or heating to form cross-linkage, and their physical properties (hardness, elasticity, and swelling) were measured. The hydrogels formulated with organic acid (1, 3, and 5%) were analyzed by spot assay against L. monocytogenes. κ-carrageenan formed hydrogels with high hardness without other cross-linkers, but they had low elasticity. The elasticity was improved by mixing with other cross-linkers such as K+ or other polymer, especially in 3% κ-carrageenan. CMC hydrogel was formed by adding cross-linkers Al3+, Na+, or Ca2+, especially in 5% CMC. Thus, stickiness and swelling for selected hydrogel formulations (two of κ-carrageenan hydrogels and three of CMC hydrogels) were measured. Among the selected hydrogels, most of them showed appropriate hardness, but only 3% κ-carrageenan-contained hydrogels maintained their shapes from swelling. Hence, 3% κ-carrageenan+0.2% KCl and 3% κ-carrageenan+1% alginate+0.2% KCl+0.2% CaCl2 were selected to be formulated with lactic acid, and showed antilisterial activity. These results indicate that 3% κ-carrageenan hydrogels formulated with lactic acid can be used to control L. monocytogenes on food surface.