To understand how reactivity between reinforcing nanoparticles and aqueous solution affects electrodeposited Cu thin films, two types of commercialized cerium oxide (ceria, CeO2) nanoparticles were used with copper sulfate electrolyte to form in-situ nanocomposite films. During this process, we observed variation in colors and pH of the electrolyte depending on the manufacturer. Ceria aqueous solution and nickel sulfate (NiSO4) aqueous solutions were also used for comparison. We checked several parameters which could be key factors contributing to the changes, such as the oxidation number of Cu, chemical impurities of ceria nanoparticles, and so on. Oxidation number was checked by salt formation by chemical reaction between CuSO4 solution and sodium hydroxide (NaOH) solution. We observed that the color changed when H2SO4 was added to the CuSO4 solution. The same effect was obtained when H2SO4 was mixed with ceria solution; the color of ceria solution changed from white to yellow. However, the color of NiSO4 solution did not show any significant changes. We did observe slight changes in the pH of the solutions in this study. We did not obtain firm evidence to explain the changes observed in this study, but changes in the color of the electrolyte might be caused by interaction of Cu ion and the by-product of ceria. The mechanical properties of the films were examined by nanoindentation, and reaction between ceria and electrolyte presumably affect the mechanical properties of electrodeposited copper films. We also examined their crystal structures and optical properties by X-ray diffraction (XRD) and UV-Vis spectroscopy.

This study was performed with an aim to improve the early-age strength of concrete containing fly ash, which is known to increase the long-age strength of concrete, reduce drying shrinkage, and enhance water tightness. The composition was partially substituted with calcium sulfoaluminate (CSA), from which ettringite is actively produced, in the early stages of hydration to verify its effect on improving the early-age strength and to determine the optimal mixing ratio. For this purpose, up to 30 % of the cement weight was substituted with fly ash, and the amount of CSA substitution was 8% of the fly ash weight. The mixtures were then fabricated into concrete specimens for compressive strength measurement and analysis of the correlation between the hydration products and the compressive strength.

Tungsten oxide films were prepared by an electrochemical deposition method for use as the anode in rechargeable lithium batteries. Continuous potentiostatic deposition of the film led to numerous cracks of the deposits while pulsed deposition significantly suppressed crack generation and film delamination. In particular, a crack-free dense tungsten oxide film with a thickness of ca. 210 nm was successfully created by pulsed deposition. The thickness of tungsten oxide was linearly proportional to deposition time. Compositional and structural analyses revealed that the as-prepared deposit was amorphous tungsten oxide and the heat treatment transformed it into crystalline triclinic tungsten oxide. Both the as-prepared and heat-treated samples reacted reversibly with lithium as the anode for rechargeable lithium batteries. Typical peaks for the conversion processes of tungsten oxides were observed in cyclic voltammograms, and the reversibility of the heat-treated sample exceeded that of the as-prepared one. Consistently, the cycling stability of the heat-treated sample proved to be much better than that of the as-prepared one in a galvanostatic charge/discharge experiment. These results demonstrate the feasibility of using electrolytic tungsten oxide films as the anode in rechargeable lithium batteries. However, further works are still needed to make a dense film with higher thickness and improved cycling stability for its practical use.

Fe4[Fe(CN)6]3 coated on a mica or TiO2/mica surface as infrared reflective blue pigment was prepared by a hydrothermal method. Fe4[Fe(CN)6]3, used as coloring agent, was uniformly coated on mica or TiO2/mica under the optimized condition of a 1.2 : 1 weight ratio between iron(III) chloride hexahydrate and potassium ferrocyanidetrihydrate at the initial pH level of 4.5 at 70˚C. The infrared (IR)-reflective pigments were characterized by SEM, Zeta-potenial, FT-IR, and UV-VIS NIR spectrophotometry. Especially the CIE color coordinate and total solar reflectance(TSR) properties of the pigments were investigated in relation to variation of the coating and coated substrate thicknesses. Isolation-heat paint was prepared with 20 wt% blue pigments fully dispersed in acryl-urethane resin and several additives to coat the film uniformly. The films were also measured with CIE color coordinate, TSR, and the surface temperature was recorded by an isolation-heat measuring system. The pigments and films of Fe4[Fe(CN)6]3 coated on mica and TiO2/mica showed high TSR values compared with the TSR value of Fe4[Fe(CN)6]3 itself. According to the increase of TSR value, the property of isolation-heat is effective. To realize the optimal blue color, we applied the the pigment to TiO2 coated mica(TM(b)) which has blueish interference color. The pigment of Fe4[Fe(CN)6]3 coated on TM(b) shows a strong blue color compared with that of Fe4[Fe(CN)6]3 coated on TiO2/Mmca(TM(w)), which has a whitish interference color.

We present an easy method of preparing two-dimensional (2D) periodic hollow tin oxide (SnO2) hemisphere array gas sensors using polystyrene (PS) spheres as a template. The structures were fabricated by the sputter deposition of thin tin (Sn) metal over an array of PS spheres on a planar substrate followed by calcination at an elevated temperature to oxidize Sn to SnO2 while removing the PS template cores. The SnO2 hemisphere array structures were examined by scanning electron microscopy and X-ray diffraction. The structures were calcined at various temperatures and their sensing properties were examined with varying operation temperatures and concentrations of nitric oxide (NO) gas. Their gas-sensing properties were investigated by measuring the electrical resistances in air and the target gases. The measurements were conducted at different NO concentrations and substrate temperatures. A minimum detection limit of 30 ppb, showing a sensitivity of S = 1.6, was observed for NO gas at an operation temperature of 150˚C for a sample having an Sn metal layer thickness corresponding to 30 sec sputtering time and calcined at 600˚C for 2 hr in air. We proved that high porosity in a hollow SnO2 hemisphere structure allows easy diffusion of the target gas molecules. The results confirm that a 2D hollow SnO2 hemisphere array structure of micronmeter sizes can be a good structural morphology for high sensitivity gas sensors.

2001년 4월 30일 국가인권위원회법안이 국회를 통과함으로써 국가인권위원회(이하 ‘인권위’)는 탄생하였다. 인권위는 유엔의 지침에 따라 인권의 보호와 증진 기능, 침해조사·구제권한. 법제개선권고권한, 인권교육권한 등을 가진 독립기관으로 설립되었다. 인권위가 국민의 인권의식을 신장하는데 기여하였다는 것은 누구도 부인할 수 없다. 그 동안 인권위는 집회 및 시위의 자유, 사상과 양심의 자유, 노동자의 권리, 외국인 동성애자 등 사회적 소수자의 인권문제에 관하여 권고나 의견표명을 하며 사회적 문제에 관하여 관심을 가져왔다. 그리고 인권침해 및 차별행위에 대하여 국민이 진정을 하게 되면 이를 조사하고 구제함으로써 국가공권력에 의한 인권침해를 감시·예방하고자 하였다. 국가인권위원회법 제1조에 따라 설립된 인권위는 모든 개인이 가지는 불가침의 기본적 인권을 보호하고 그 수준을 향상시킴으로써 인간으로서의 존엄과 가치를 구현하고 민주적 기본질서의 확립에 이바지하는 데 기여하여야 한다. 진정의 대상에 대해서는 인권전반의 문제를 다루고, 보다 강제력 있는 조사권을 부여함과 동시에 조사권 제한 범위를 적절히 조정하고 분명히할 필요가 있다. 인권침해 구제를 위해서는, 사안에 따라서 시정명령권이나 다양한 구제조치를 취할 수 있도록 해야 한다. 정부에 대하여 조언하고 권고하는 기능은 인권침해 조사와 구제 못지않게 중요한 기능이다. 국가인권기구는 단지 정부의 업무를 지원하는 차원이 아니라, 적극적이고, 독립적으로 인권관련 법안과 법률 등을 검토하고, 개선을 권고하며, 정부의 관행을 개선하는데 노력하여야 한다. 그러한 과정 속에서 인권위는 더욱 신뢰를 얻을 수 있고, 조직과 예산상 확충도 실현할 수 있을 것이다.

중국은 2002년 이래 한국의 최대 투자대상국이다. 1979년 최초로 외상투자기업법이 제정된 이래 중국의 외국인투자관련 법률제도가 끊임없이 개선되어 왔지만 입법속도가 투자환경의 변화속도에 미치지 못하고 있다. 이에 본 연구는 현재 중국의 외상투자기업법률에 존재하는 주요 이슈들을 다양한 사례분석을 통해 살펴보고 향후 발전방향을 전망하고자 한다. 본문에서는 중국 회사법을 외상투자기업에 적용하면서 발생하는 다양한 문제를 사례와 함께 분석하고, 그 외 지방보호주의, 이사회의 직권문제, 출자 사기, 사회보장기금 납입문제 등의 문제도 다루고 있다. 중국은 기업의 형식에 따라 외국인투자기업에 관한 법률을 각각 제정하고 있어 많은 부분에서 중복되고 때로는 충돌되는 부분도 있다. 이 때문에 기업법 위주의 외국인투자법 체계는 향후 개선될 것으로 전망된다.

한국의 현행 대외무역법은 1986년 제정된 이래 29차례의 개정을 거치면서 무역의 확대와 국제수지의 균형에 기여하였다. 반면 1994년 제정된 이래 중국의 대외무역법은 WTO가입 이후 2004년 한 차례의 개정만을 거쳤으며 현재 변화하는 국제무역환경에 대응하기 위해 개정논의가 진행중에 있다. 한중 양국의 역사적, 사회적 배경이 다르기 때문에 양국의 대외무역법 체계는 구성요소와 특징 면에서 차이가 있다. 이러한 양국 대외무역법의 역사적 발전과정에 대한 이해는 중국의 현행 대외무역법에 대한 이해를 제고시킬 뿐만 아니라 향후 개정방향에도 시사점을 제공할 수 있을 것이다. 본 연구에서는 한국과 중국의 대외무역법의 역사적 발전과정을 살펴본 후, 양국 대외무역법의 발전배경, 행정관리체계, 무역촉진 및 무역제한, 수출입질서, 무역업 자유화 등을 비교분석하고 있다.

수치심으로 인해 사람들은 자신을 부정적으로 보기 싶다. 다시 말하면 내재화된 수치심으로 인해 사람들은 자신은 가치 없는 존재로 생각하기 쉽다. 또한 기독교 안에 있는 죄의 신학 역시 사람들로 하여금 죄책감을 느끼게 하면서 그 안에는 자신에 대한 수치심을 동반하게 만들었다. 코헛의 자기 심리학적 이해 속에서 수치심은 자아와 자아 대상과의 관계성 속에서의 수치심이 생성될 수 있는 자아의 개념 설명으로 인해 자아 대상의 적절한 공감은 건강한 자아를 형성하고 수치심을 덜 느낄 수 있음을 시사한다. 또한 죄의 신학의 절대적 기준이 많은 힘없는 자들에게는 더욱 더 죄책감을 느끼게 하기도 하기 때문에 화를 내고 불순종하고 자만이 죄라고 정의하기에 앞서 그들이 왜 그러한 감정을 가지게 되었는지에 대한 탐구에 새로운 죄의 해석을 제공함으로써 약한 자들이 느끼는 죄책감으로 인한 수치심을 극복하도록 도와주고자 한다.

중국은 2010년 ‘涉外民事關係法律適用法’을 제정하여 2011년 4월부터 시행하고 있다. 본 법의 제정으로 형식적 측면에서 각 법에 분산되어 있던 국제사법 규정들이 완전하지는 않지만 본 법을 중심으로 재편되는 계기가 되었다. 본 법은 내용적 측면에서도 선진입법기술을 대폭적으로 수용하여 개방적인 태도를 취하였는데, 특히 최밀관련국법 원칙을 도입한점, 기존의 본국법주의를 포기하고 상거소지개념을 전격적으로 도입한점, 당사자자치 원칙을 제1장 일반규정에 규정하여 국제사법의 일반원칙으로 선언한 점은 전위적 입법으로서 세계적인 국제사법의 동향을 반영한 것이라고 평가되고 있다. 중국 국제사법의 시행으로 인해 중국에서 활동하고 있는 우리나라 기업이나 교민들이 법적 분쟁에 휘말리게 된 경우, 중국의 섭외성 판단 기준에 따라 당해 사건이 섭외사건으로 판단되면 중국 국제사법에 따라 준거법이 결정될 것이다. 또한 우리 국제사법 제9조가 반정(renvoi )을 인정하고 있으므로, 한국 법원에서 문제된 섭외적 사안에 대하여 우리 국제사법의 적용을 통해 중국법이 준거법으로 지정된 경우에는 중국 국제사법에 의해 한국법이 준거법으로 결정되는지를 반드시 확인하여야 한다. 그러한 점에서 중국 국제사법에 관한 연구는 이론적ᆞ실제적으로 큰 의의가 있다. 본고에서는 중국 국제사법의 제정경과 및 체계적 위상에 대해 검토한후에, 중국 국제사법의 입법적 의의로서 최밀관련국법 원칙, 당사자자치, 상거소지법주의의 전면적 도입에 관하여 살펴보았다.

This report describes a rare case of primary rectal mature teratoma in a 56-year-old woman. She was referred to the outpatient clinic with a large pedunculated rectal mass, which was found during a regular health check-up. Polypectomy was performed and microscopic findings showed various structures derived from all three germ cell layers. Epidermis, hair follicles, sebaceous glands, eccrine glands, and apocrine sweat glands, with some scattered melanophages and lymphocytes were present as ectodermal derivatives. Smooth muscle fibers, blood vessels, and fibrous and adipose tissues were found as mesodermal derivatives. In addition, thyroid follicles, mucinous glands, and bronchial respiratory epithelium with peribronchial glands were detected as endodermal derivatives. She is healthy and has shown no evidence of recurrence or distant metastasis for 25 months post-surgical resection. Primary rectal teratomas are generally benign and primarily affect women. Therefore, minimally invasive surgical procedures, such as endoscopic polypectomy for a pedunculated polyp and segmentectomy for a larger mass, are satisfactory in most cases. Induction of primary rectal teratomas has been suggested to occur mainly by errors in a single germ cell after the end of meiosis I; in addition, it has also been suggested that the difference in gender incidence may be associated with differences in sex chromosomes between males and females rather than with anatomical proximity between ovary and rectum.

The anti-proliferative efficacy of t,t-conjugated linoleic acid (t,t-CLA), c9,t11-CLA, and t10,c12-CLA was compared in several human cancer cell lines. Gastric NCI-N87, liver Hep3B, pancreas Capan-2, and lung NCI-H522 cancer cells were incubated with 50 μM CLA isomers over a period of 6 days. The t,t-CLA inhibited the growth of all cancer cell lines to different extents, but c9,t11-CLA and t10,c12-CLA inhibited or stimulated the growth of the cancer cell lines. NCI-N87 cells were the most sensitive to growth inhibition and apoptosis from all CLA isomers tested. In NCI-N87 cells, CLA isomers reduced the release of arachidonic acid (AA) via the inhibition of cytosolic phospholipase A2 (cPLA2 ) activity, consequently reducing the production of PGE2 through the inhibition of cyclooxygenase-2 (COX-2). The efficacies of CLA isomers were in the following order (from most to least effective): t,t-CLA, t10,c12-CLA and c9,t11-CLA. Overall, these results imply that the anti-proliferative efficacy of t,t-CLA on cancer cells, especially NCI-N87 cells, was greater than other CLA isomers due to its induction of apoptosis through the inhibition of cPLA2 and COX-2 activities.

Antimicrobial peptides (AMPs) are an important component of innate defense mechanisms with broad-spectrum activities against various pathogenic microorganisms, including Gram-positive and Gram-negative bacteria, fungi, and viruses. Antibiotic resistance has become a pervasive and global health burden, resulting in the immediate need to develop a new class of antibiotic substances. We screened a 16-mer random peptide library using the yeast two-hybrid system with Beclin 1 as bait and found that two 16-mer peptides (named P4 and P30) appeared to interact with Beclin1 in the β-gal assay. The two candidate cDNAs were introduced into the yeast secretory system of Pichia pastoris and their expression induced in the presence of methanol. Spectrophotometric analysis and Disc clear zone assay using the supernatant of the yeast growth media showed that both of the two peptides had strong activities against Staphylococcus aureus, MRSA (methicillin resistance Staphylococcus aureus), MRSA2242, and MRSA-2250, but no effect on commensal Lactobacillus strains. PCR analysis of the genomic DNA of transformed Pichia pastoris using AOX1 primers revealed that the two cDNAs were integrated into the genome at the AOX1 locus. Our result suggests that these peptides could be developed as a useful alternative to classic chemical antibiotics.

To clarify the role of stem cells in hepatocarcinogenesis, CD44 expression was investigated in mouse livers as well as embryonic cell lineages treated with diethylnitrosamine (DEN). Liver tumors induced by DEN were analyzed by immunohistochemisty for CD44. Liver tissues were sampled at 6, 24, and 48 hr after treatment with saline or DEN. Mouse embryonic stem cells (ESCs), hepatic progenitor cells (HPCs), and hepatocyte like cells (HCs), representing 0, 22, and 40 days of differentiation, respectively, were treated with DEN at four doses (0, 1, 5, and 15 mM, respectively) for 24 hr, after which CD44 expression levels were examined by relative quantitative real-time PCR. CD44 expression was weakly detected in tumor cells as well as in some hepatocytes surrounding the tumor cells. However, CD44 expression was not detected in liver tissue treated with DEN at early time points. The CD44 mRNA expression level was significantly different among cells treated with 5 mM DEN at day 22 (P<0.01) as well as 1, 5, and 15 mM DEN at day 40 (P<0.01) compared with control. Taken together, CD44 expression slightly increased in mouse DEN-induced tumors. Furthermore, expression of CD44 in embryonic cell lineages treated with various doses of DEN significantly differed among embryo stem cells and derived hepatic lineage cells. This suggests that CD44 expression may be modulated in the progeny of stem cells during their differentiation toward hepatocytes, and its expression may increase in the tumor stage but not during early carcinogenesis.

The purpose of this study was to identify the composition and organization of lingual tissues underlying the histo-structural change of developing tongue in Korean native goats by light microscopy (LM). Tongues of the fetuses on days 60, 90, 120 and neonate were examined for the morphological development. In the 60-day-old fetuses, the tongue tissues were differentiated into epithelium, lamina propria and muscle layer. Primordia of filiform, conical, lentiform, fungiform and vallate papillae appeared and rudiments of taste bud were observed in the epithelia of the primordia of the gustatory papillae. The dorsal surface of the lingual epithelia showed a weak PAS positive reaction. Collagenous fibers and small blood vessels were shown in the connective tissues. In the 90-day-old fetuses, Von Ebner’s glands were moderately PAS positive while the muscle fibers and connective tissue were strongly positive for PAS. The collagenous fibers increased and came to have a more complex arrangement in the tongue. The muscle fibers were spread out at various directions and developed in striated muscle bundles. In the 120-day-old fetuses, taste buds were observed in the epithelia of the gustatory papillae, and several well-developed tissues visible such as blood vessels, collagenous fibers, muscle fiber bundles and Von Ebner’s glands. In the neonates, many taste buds were found in a transverse section of the vallate papilla. The muscle layers, Von Ebner’s glands, collagenous fibers and blood vessels were more developed than those of the 120-day old fetuses. These findings indicate that goat tongues have a variety of different shapes during prenatal development.

Our previous research on sulfated polysaccharide purified from Ecklonia cava, a brown alga found in Jeju island, Korea, showed that sulfated polysaccharides modulate the apoptotic threshold of intestinal cells, thereby preventing intestinal damage caused by ionizing radiation. In this study, we investigated the ability of sulfated polysaccharide to augment restoration of small intestinal stem cells from γ-ray-induced damage. In our results, sulfated polysaccharide treatment increased the numbers of Ki-67-positive cells as well as inducible nitric oxide synthase (iNOS)-expressing cells in the small intestine compared with those of irradiated only mice. Meanwhile, exposure to irradiation increased the number of paneth cells, which are frequently associated with intestinal inflammation, whereas sulfated polysaccharide treatment reduced the number of paneth cells in the small intestinal crypt. Conclusively, our data suggest that reduction of iNOS-expressing cells and paneth cells in sulfated polysaccharide-treated mice contributes to the inhibition of radiation-induced intestinal inflammation.

This study was conducted in order to determine the functionality of mineral-rich salt with lower NaCl and higher mineral contents on blood pressure and lipid metabolism in Dahl salt-sensitive rats. A 1% salt solution was administered to five-week-old male Dahl rats– one normal and three salt groups (Purified salt, sun-dried salt, and bamboo salt) for 15 weeks. On the basis of the salt production process, the sun-dried group was classified into two subgroups: SS1 (2-year) and SS2 (>5-year) depending on the storage period of the mineral-rich salt. The relationships between salt intake and changes in blood pressure, serum lipids, and serum mineral concentrations were then examined. The results showed that intake of SS2, which is stored for five years, and BS (bamboo salt) resulted in continuous delay of the increase in blood pressure and inhibited angiotensin–converting enzyme (ACE) activity. In addition, a significant decrease in the triglyceride level in serum lipids of approximately 30% was observed in the SS2 group compared to the PS (purified salt) group. However, all salt intake groups showed an increase in total cholesterol levels compared to the normal group. The results demonstrate that intake of mineral-rich salt is beneficial for the human body and results in reduced blood pressure and triglyceride levels in serum lipids, however, conduct of more research will be needed in order to explore other functions.

입증책임이란 요건사실의 진위가 불명한 경우에 대처하여 요건사실의 부존재의 경우와 마찬가지로 취급하여 소송당사자 일방에 대하여 그에게 유리한 법규부적용의 불이익을 부담시켜서 판결을 가능하게 하는 것이다. 따라서 소송당사자의 승패에 결정적 역할을 한다. 이와 관련하여 상속이나 증여의 경우에는 납세자에게 자료가 편중되어 있어서 즉 증거의 거리와 증거의 지배를 대부분 납세의무자가 하고 있어서 과세관청에 입증의 곤란을 초래하기 때문에, 이러한 입증책임의 곤란의 문제를 해결하기 위해 다른 세법의 규정과 달리 많은 추정규정과 의제규정 두고 있다. 하지만 추정규정과 의제규정은 과세관청의 입증책임을 완화 또는 입증필요의 전환을 시키는 점에 있어서 입증책임분배원칙에 대한 예외에 해당할 수 있다. 따라서 이와 같은 입법은 되도록 제한할 필요가 있고, 그 해석도 엄격할 필요가 있다. 즉 납세의무자의 헌법 제23조의 재산권과 헌법 제27조의 재판받을 권리를 침해할 가능성을 내포하고 있는 점에서 국민의 예측가능성을 고려한 입법이 요구된다. 궁극적으로 조세법률주의를 실질적으로 잠탈 할 가능성이 있으므로 충분한 고려를 통한 입법이 요구된다. 특히 의제규정의 경우에는 추정규정과 달리 반증을 통한 번복 가능성이 존재하지 않으므로 국민의 구제가능성 어렵기 때문에 신중한 입법이 요구된다. 특히 명의신탁의 증여의제규정인 상증법 제45조의2와 관련하여 위헌가능성과 최근 대법원 판례의 ‘조세회피의 목적’과 관련된 변화된 입장을 고찰할 필요가 있다. 그리고 추정규정 중 재산취득자금의 증여추정 규정인 상증법 제45조 1항에서의 입증의 범위에 관하여 종전의 규정과 달리 ‘다른 자로부터’의 문구가 삭제되어 기존과 다른 입증책임론이 필요한 것이 아닌지 고찰할 필요가 있다.

흔히 도교(道敎)는 여러 가지 신체수련을 통해 장생불사를 추구하고 부적이나 주문, 의례를 통해 세속적 구복을 추구하는 종교로서, 노장사상과 같은 도가(道家)와 구별하여야 하는 것으로 인식되는 경우가 많다. 물론 도교 안에는 벽곡(辟穀)과 같은 식이요법, 도인(導引)과 같은 체조, 화학연금술, 약초학, 부적 등 다양한 종교 문화적 요소가 존재한다. 그것만이라면 도교는 의학이나 체육학, 혹은 대중적 주술행위에 지나지 않을 것이다. 그러나 제대로 살펴보면 도교 안에는 깊은 사색과 명상이 있고, 그들의 심신 훈련의 기술의 바탕에는 매우 심오하고 체계적인 철학이 자리하고 있다. 노장사상으로부터 출발된 도교의 다양한 사상과 수행법은 당말 오대 이후로부터 시작되어 송원 시대에 가장 흥성했던 내단 사상에 이르러 가장 완성도 있게 체계화된다. 도교 내단(內丹)의 가장 독특한 수행법의 특징은 몸과 마음의 균형 있는 연마, 곧 ‘성명쌍수(性命雙修)’라고 할 수 있다. 유교나 불교와 구별되는 도교수행의 독특성은 마음수련을 위해 몸 수련이 반드시 동반되어야 한다는 것이다. 도교에서는 인간을 심신상관적(psycho-somatic)인 존재로 인식하는 바, 도교의 구원, 곧 득도를 통해 신선적 경지에 오르기 위해서는 육체와 정신, 양면의 수행이 필요하다고 본다.도교의 내단 사상과 수행법은 한국에서도 성행하였다. 조선시대의 여러 선비들은 단학수행에 몰두하고 다양한 저술을 통해 그들의 수행법을 소개하고 있다. 그 대표적인 인물이 김시습(金時習)․권극중(權克仲)․정염(鄭磏) 등이다. 조선중기의 정염(鄭磏: 1506~1549)에 의해 작성된『용호비결(龍虎秘訣)』은 조선시대의 내단內丹)사상의 핵심을 잘 보여주는 저서이다『용호비결』은 도교의 심신수련서 가운데에서도 그 내용이 간단하면서도 한국도교의 내단 수행(修行)의 본질과 핵심을 잘 드러내주고 있다.『용호비결』에서는 도교의 수행법을 폐기(閉氣), 주천화후(周天火侯), 태식(胎息)의 세 단계로 나누어 설명하고 있다. 이 가운데에서도 폐기의 과정이 가장 중요하며 따라서 이를 올바로 이해하고 바르게 수련하는 것이 필요하다고 한다. 『용호비결』과 같은 단학 수행서를 통해서 알 수 있듯이 도교의 수행법은 정신완성을 위해 신체의 상태를 온전하게 보전하고 그 순환상태가 원활하게 함으로써 지속적으로 정신에너지를 공급하고자 한다. 그리고 이러한 심신상관적 인간이해와 종교적 실천은 현대의학이나 심리학에서 말하는 인간의 정신이해와도 상통한다고 할 수 있다.

In this study, characteristics and immuno-modulatory effects of Weissella cibaria JW15 isolated from Kimchi, traditional Korean fermented food, were examined for investigation of the capacity of potentially probiotic strains. We measured acid, bile, and heat tolerance, adhesive properties to intestinal epithelial cells, and inhibitory activity against pathogens. JW15 could survive at pH 3.0 for 2 hr, but not at pH 2.0. JW15 also showed tolerance to 0.3% oxgall bile salt, and heat tolerance at 70°C and 80°C for 5 min, respectively. Adhesive ability to Caco-2 cells was similar to that of Lactobacillus rhamnosus GG (LGG), a well-known commercial probiotic. JW15 exhibited antimicrobial activities to pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, and Salmonella enteritidis. The immuno-modulatory effects of JW15 were compared with those of LGG, a well-known immune enhancer. For analysis, production of nitric oxide (NO), NF-κB (Nuclear factor κB), Interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) was measured. The concentration of NO induced by JW15 was higher than that by LGG at low concentration (1 × 107 cfu/mL). Low and high (5 × 107 CFU/mL) concentration of JW15 induced statistically higher production of NF-κB, IL-1β, and TNF-α than that produced by LGG, respectively. In conclusion, Weissella cibaria JW15 had ability as a probiotic strain, including acid, bile, and heat tolerance, adhesive properties to intestinal epithelial cells, and inhibitory activity against pathogens. In addition, JW15 showed better immuno-modulatory effects than LGG when NO, NF-κB, IL-1β, and TNF-α were measured. According to these results, the characteristics and immunomodulating activity of Weissella cibaria JW15 are suitable for consideration as a potential probiotic.