The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

PURPOSES : There has been increasing interest in South Korea on warm-mix asphalt (WMA) and cold-mix asphalt (CMA) technologies that allow production of asphalt pavement mixtures at comparatively lower temperatures than those of hot-mix asphalt (HMA) for use in pavement engineering. This study aims to evaluate the feasibility of replacing HMA pavement with WMA pavement with the goal of reducing CO2 emissions associated with asphalt production for road construction. METHODS : Changes in the dynamic modulus characteristics of WMA and HMA according to short-term and long-term aging were evaluated. In addition, the effects of water damage were evaluated for short- and long-term aging stages. RESULTS : For WMA, in the process of mixing and short-term aging, early-age dynamic modulus decreased owing to low temperature and reduced short-term aging (STA) time. This could result in early damage to the asphalt pavement depending on the applied traffic load and environmental load. CONCLUSIONS : Mastercurves of the dynamic modulus were used for comparative analysis of WMA and HMA. Compared to the dynamic modulus after STA of HMA, the estimated aging time determined by experiments for WMA to achieve the required stiffness was more than 48 hours, which is equiva-lent to approximately 4 to 5 years real service life when converted. It is considered that further studies are needed for performance optimization to achieve early-age performance of the asphalt mixes.

This longitudinal study examined how an English for Specific Purposes (ESP) teacher transformed her teacher identity over time. Hinging on the close relationship between teaching and identity construction, this study was grounded on the notion that teaching a language is becoming a person who teaches it (Benson, 2017). Data were collected through six face-to-face interviews and six electronic interviews with the teacher during a four-year period. Findings demonstrate that becoming an ESP teacher is a complex process of constructing and negotiating identities. The teacher started ESP teaching as a scared general English teacher, and then negotiated her identities as a struggling but hardworking collaborator and learner. Later, she positioned herself as a novice ESP teacher, and then finally constructed her identity as a competent, helpful ESP teacher. Significant factors affecting identity transformation include needs analysis, enhanced subject matter knowledge, capacity to develop teaching materials, and teacher agency. Based on these findings, this study suggests implications for ESP teacher education programs.

본 연구에서는Stable Diffusion 프레임워크를 활용하여 게임 스타일의 스케치, 특히 도시 장면을 생성하는 방법 을 소개한다. 확산 기반의 모델인Stable Diffusion은 쉬운 접근성과 뛰어난 성능으로 많은 연구자와 일반인들에 게 선호되며, 텍스트-스케치, 이미지-스케치의 생성이 가능하다. Stable Diffusion의 몇 가지 문제는 이미지의 국 소성 보존 문제 및 미세 조정인데, 이를ControlNet과DreamBooth를 사용하여 해결한다. 결과적으로, 본 연구를 통 해 게임 제작에 사용될 수 있는 텍스트-스케치, 이미지-스케치 생성이 가능하며, 더 나아가 아티스트를 돕는 툴 로도 활용될 수 있다.

Emojis and avatars are widely used in online communications, but their emotional conveyance lacks research. This study aims to contribute to the field of emotional expression in computer-mediated communication (CMC) by exploring the effectiveness of emotion recognition, the intensity of perceived emotions, and the perceived preferences for emojis and avatars as emotional expression tools. The following were used as stimuli: 12 photographs from the Yonsei-Face database, 12 Memojis that reflected the photographs, and 6 iOS emojis. The results of this study indicate that emojis outperformed other forms of emotional expression in terms of conveying emotions, intensity, and preference. Indeed, the study findings confirm that emojis remain the dominant form of emotional signals in CMC. In contrast, the study revealed that Memojis were inadequate as an expressive emotional cue. Participants did not perceive Memojis to effectively convey emotions compared with other forms of expression, such as emojis or real human faces. This suggests room for improvement in the design and implementation of Memojis to enhance their effectiveness in accurately conveying intended emotions. Addressing the limitations of Memojis and exploring ways to optimize their emotional expressiveness necessitate further research and development in avatar design.

본 논문은 예이츠와 셰익스피어가 문학적 도구로서 ‘유령’을 사용함에 있어 서로 다른 입장과 목적을 갖고 있었음을 전제로 그들이 극작품에서 ‘유령’이라는 도구를 어떻게 사용하는지에 대하여 살펴보고자 한다. 셰익스피어 유령극의 전형적인 특징은 유령을 마주하게 되는 인물이 대부분 죄를 지은 인물이라는 점인데, 특정 유령을 사용함으로써 캐릭터의 심리에 대해 많은 것을 드러내고, 이를 통하여 관객들의 공감적 반응을 이끌어낸다. 반면, 예이츠는 유령을 마주하는 인물보다는 유령 그 자체가 목적이었기 때문에 셰익스피어와는 유령에 대한 사용방식이 다를 수밖에 없었다. 예이츠는 극작가로서 그 자신만의 가치를 갖고 있었다.

본 연구에서는 자기공명영상 검사 시 와인색 반영구 문신용 염료가 신호 강도에 미치는 영향을 분석하고, 전자파흡수 에 의한 발열 감소 방안을 연구하고자 하였다. 한천과 와인색 반영구 문신용 염료를 이용하여 염료가 퍼진 형태의 팬텀(패드형 팬텀)과 한곳에 뭉친 형태의 팬텀(주사형 팬텀)을 제작하였다. 두 팬텀은 전신용 자기공명영상장치에서 고속스핀에코(turbo spin echo, TSE)와 경사에코(gradient echo, GRE) 계열의 펄스열을 이용하여 2D와 3D로 검사하였다. 검사 후 영상 분석은 팬텀의 몸체, 염료, 공기 방울, 배경을 대상으로 총 720회의 신호강도를 측정하였 다. 전자파흡수에 의한 발열 감소 방안을 확인하기 위해 삽입형 온도계를 팬텀에 삽입 후 검사 전후 팬텀의 온도 변화를 확인하였다. 이때, 대기 온도의 상승 변화를 고려하기 위해 검사 전 대기 온도를 측정하였고, 발열 감소를 위한 방법으로 장치에 내장된 팬, 젖은 거즈, 얼음을 이용하였다. 신호 강도는 2D 영상보다 3D 영상이 좋았고, TSE 계열이 GRE 계열보다 좋았다. 특히 와인색 반영구 문신용 염료가 있는 부위의 신호강도는 공기 때문에 발생한 자화 율 인공물보다 신호강도가 낮게 측정되었다. 온도 측정 검사는 패드형 팬텀과 주사형 팬텀 모두 검사에 의한 온도상 승 효과가 있었으며 온도의 저감을 위한 방법으로 얼음 패드, 팬, 젖은 거즈 순서였다. 본 연구를 통해 와인색 반영구 문신용 염료는 자기공명영상 검사에서 영상과 환자의 안전에 문제를 일으킬 수 있음을 확인하였고, 발열 감소 방안으 로 얼음 패드가 효과적이었다는 것을 확인할 수 있었다.

The rack cylinder is an important part of the pile leg structure of the jack up platform. Because of its complex structure, the flow field around the rack cylinder is different from that around the ordinary cylinder, which brings difficulties to the research of the rack cylinder. In this paper, using CFD(Computational Fluid Dynamics) solved the flow field of chords with different rack height and rack width under different KC and Re, the characteristics of the flow field around the cylinder with rack are obtained. The results show that Re, KC, rack height and rack width all have different effects on the flow field. When Re and KC are constant, Cd will increase with the increase of rack height ratio, the change of Cd and Cl is not significant, while the change of Cd and Cl varies with Re when the chord structure is fixed.

In this study, we examined the antagonistic effects of sprout-borne lactic acid bacteria (LAB) on Salmonella enterica serovar Enteritidis. This antagonism is promoted as a means of controlling contamination during sprout production and provides additional LAB for consumers. We isolated a total of 24 LAB isolates in nine species and five genera from seven popular vegetable sprouts: alfalfa (Medicago sativa), clover (Trifolium pratense), broccoli (Brassica oleracea ssp. italica), vitamin (B. rapa ssp. narinosa), red radish (Raphanus sativus), red kohlrabi (B. oleracea var. gongylodes), and Kimchi cabbage (B. campestris var. pekinensis). Based on 16S rRNA gene sequences, the LAB species were identified as Enterococcus casseliflavus, E. faecium, E. gallinarum, E. mundtii, Lactococcus taiwanensis, Leuconostoc mesenteroides, Pediococcus pentosaceus, and Weissella cibaria, and W. confusa. A total of 16 LAB isolates in seven species including E. faecium, E. gallinarum, E. mundtii, L. taiwanensis, L. mesenteroides, P. pentosaceus, and W. cibaria showed antagonistic activity toward S. enterica. The growth inhibition of sprout LAB on S. enterica was confirmed by co-culture. Unexpectedly, sprout LAB failed to suppress the growth of S. enterica in alfalfa sprouts, whereas all LAB strains stimulate S. enterica growth even if it is not significant in some strains. The findings of this study indicate that S. enterica-antagonistic LAB are detrimental to food hygiene and will contribute to further LAB research and improved vegetable sprout production.

Single-atom Pd clusters anchored on t-BaTiO3 material was synthesized using hydrothermal and ultrasonic methods for the effective piezoelectric catalytic degradation of pollutants using vibration energy. XRD patterns of BaTiO3 loaded with monoatomic Pd were obtained before and after calcining, and showed typical cubic-phase BTO. TEM and HAADF-STEM images indicated single-atom Pd clusters were successfully introduced into the BaTiO3. The piezoelectric current density of the prepared Pd-BaTiO3 binary composite was significantly higher than that of the pristine BaTiO3. Under mechanical vibration, the nanomaterial exhibited a tetracycline decomposition rate of ~95 % within 7 h, which is much higher than the degradation rate of 56.7 % observed with pure BaTiO3. Many of the piezo-induced electrons escaped to the Pd-doped BaTiO3 interface because of Pd’s excellent conductivity. Single-atom Pd clusters help promote the separation of the piezo-induced electrons, thereby achieving synergistic catalysis. This work demonstrates the feasibility of combining ultrasonic technology with the piezoelectric effect and provides a promising strategy for the development of ultrasonic and piezoelectric materials.

Changes in the microstructure and mechanical properties of as-roll-bonded AA6061/AA5052/AA1050 threelayered sheet with increasing annealing temperature were investigated in detail. The commercial AA6061, AA5052 and AA1050 sheets with 2 mm thickness were roll-bonded by multi-pass rolling at ambient temperature. The roll-bonded Al sheets were then annealed for 1 h at various temperatures from 200 to 400 °C. The specimens annealed up to 250 °C showed a typical deformation structure where the grains are elongated in the rolling direction in all regions. However, after annealing at 300 °C, while AA6061 and AA1050 regions still retained the deformation structure, but AA5052 region changed into complete recrystallization. For all the annealed materials, the fraction of high angle grain boundaries was lower than that of low angle grain boundaries. In addition, while the rolling texture of the {110}<112> and {123}<634> components strongly developed in the AA6061 and AA1050 regions, in the AA5052 region the recrystallization texture of the {100}<001> component developed. After annealing at 350 °C the recrystallization texture developed in all regions. The as-rolled material exhibited a relatively high tensile strength of 282 MPa and elongation of 18 %. However, the tensile strength decreased and the elongation increased gradually with the increase in annealing temperature. The changes in mechanical properties with increasing annealing temperature were compared with those of other three-layered Al sheets fabricated in previous studies.

The legal status of Biodiversity Beyond National Jurisdiction (BBNJ) has been regulated through the High Seas Treaty as a common heritage of humankind. However, there still exist problems related to overlapping areas above the Extended Continental Shelf (ECS). In such areas, a significant continental shelf would fall within national jurisdiction, whereas the water column would be under the regime of the high seas, and BBNJ would be the common heritage of humankind. We argue that, in order to address the overlapping of areas within the superjacent waters in the ECS, a given sui generis status is required, so that the coastal state has a sovereign right to conserve the BBNJ in these areas. This study concludes that the sui generis arrangement should be implemented in the context of an overlapping ECS and its water column. The efforts of the Indonesian government to extend the continental shelf beyond 200 nautical miles constitute an important step in preserving natural resources for future generations.

Volcanic seawater has been naturally filtered and purified by volcanic rock layers. It contains abundant minerals such as calcium, magnesium, and iron. This study investigated the genotoxicity of calcium from Jeju lava seawater (CJLS). We performed bacterial reverse mutation assay, chromosomal aberration assay, and mammalian micronucleus test at up to 5,000 g/plate concentrations with or without metabolic activation to determine the CJLS genetic toxicity. None of these tests showed any mutagenic potential. The bacterial reverse mutation assay showed that the CJLS did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537, and Escherichia coli WP2uvrA with or without metabolic activation of the S9 mixture. The oral administration of CJLS also did not significantly increase the number of micronucleated polychromatic erythrocytes or the mean ratio of polychromatic to total erythrocytes. Additionally, CJLS did not cause a significant chromosomal aberration in CHL cells in the presence or absence of S9 activation. Therefore, CJLS could be considered as a reliable and safe functional food ingredient.

This study investigated the surface tension and foaming properties of the hot-water extracts of pumpkin leaf and chickpea, as well as the effects of the plant hot-water extracts on white pan bread baking. Propylene glycol alginate (PGA), a synthetic emulsifier widely used in bakery, was used as a control. Pumpkin leaf water extract showed lower surface tension and comparable foaming capacity, compared with chickpea water extract and PGA solution when total solid  0.15% (w/w). Chickpea water extract showed the highest foam stability when total solid  0.15% (w/w). The dough was found to have a weak gel structure, and its viscoelastic properties were not significantly influenced by adding 0.05% or 0.15% (w/w) (based on total solid content) plant water extracts or PGA. The specific volume of the bread increased, and the baking loss was reduced by adding the two plant water extracts of total solid 0.15% (w/w). The hardness and chewiness of the bread crumb were reduced to a level comparable to the crumb containing 0.05% (w/w) PGA. The results showed that the pumpkin leaf water extract could be an effective natural emulsifier with a high phenolic content for bakery products.

Central bank digital currency (CBDC) is generally defined as the digital form of a country’s fiat currency. Based on the distributed ledger technology and other financial technology, CBDC could improve the efficiency of domestic and cross-border payments, increase payment safety and soundness, and promote financial inclusion. However, it is argued that the introduction of CBDC would threaten data security and invade personal privacy. Currently, this issue has received growing concern, and some recommendations are proposed by countries or international organizations, like privacy design, restrictions on public authorities and payment intermediaries, and establishing independent supervisory authority. Other suggestions include getting countries involved in international coordination and promoting the formation of unified standards. Among major economies, China is the first to launch CBDC, which is known as e-CNY. Based on an overview of the privacy protection legislation in China, this article attempts to describe the rules that should be followed when dealing with personal data generated in e-CNY circulation.

행복은 인간이 추구하는 최고의 가치이자 선이다. 그러나 오늘날의 경영환경은 직원들에게 점점 더 많 은 자원을 요구하여 그들의 행복추구를 방해한다. 자원보존이론과 긍정정서의 구축 및 확장이론에 의하 면, 개인의 행복은 그들이 소유하고 있거나 획득할 수 있는 자원량에 비례하게 된다. 본 연구는 심리적 웰빙이라는 개념을 통해 조직 구성원의 행복을 살펴보고자 한다. 이 연구에서 저자는 먼저 심리적 웰빙의 정서적 측면과 자아실현적 측면을 구분한 다음, 목표 상황에 따라 이러한 측면이 어떻게 변화하는지 살펴 본다. 목표설정이론에 근거하여 목표달성을 위해 자기규제를 할 경우 이를 통해 자아실현적 측면의 심리 적 웰빙을 증진할 수 있지만, 동시에 상실되는 자원을 통해 정서적 웰빙이 침해될 수 있다. 목표가 불명확 할 경우 목표달성가능성이 줄어들어 개인은 회피적 자기규제를 통해 자원손실을 줄이게되고 이를 통해 정서적 웰빙을 보호하지만 자아실현적 웰빙은 감소할 수 있다. 본 논문은 또한 상사근접관리로 인한 강제 적 규제상황을 가정하여 조절효과를 확인하였다. 12개 조직의 데이터를 수집하여 통계적으로 검증하였다. 그 결과 목표의 난이도와 모호성은 직원의 자기조절 전략을 통해 심리적 웰빙을 변화시키며, 이러한 경향 은 상사근접관리에 의해 조절되는 것으로 나타났다. 그리고 심리적 웰빙의 이 두 가지 측면이 갖는 이론 적 및 실무적 시사점에 대해서도 논하였다.