Brassica rapa subspecies show morphological variability, containing vegetable types and oilseed types. The yellow sarson types(Brassica rapa ssp, tricolaris) have distinct morphology, yellow seeded and contain some lines with very unique character of tetralocular ovary. For genetic studies on tetralocular ovary related to high seed yields, we produced genetic segregation population with F2 and double haploid(DH) population. The yellow sarson LP8 (YS-033, CGN06835) with character of tetralocular ovary used as a maternal plant and crossed by LP21 of turnip rape type with bilocular ovary as paternal plant. We took on the microspore cultures on immature bud which is collected on sizing from 2mm to 3.2mm for DH population. The regenerations DH plants are analyzed by ploidy determination using flow cytrometer and selected on diploid plants. These regenerated DH and F2 plants are doing bud pollination and measuring the phenotype traits. Also, these populations will be used for identify of genetic locus relate to tetralocular ovary using genotyping by sequencing.

This study was conducted to measure the effect of PE film mulching and irrigation methods on the growth, yield and antioxidant activity of potatoes tubers, in order to examine the possibility of cultivating potatoes in winter season in vinyl greenhouse on the reclaimed tidal land with weak ground inside the sea wall currently completed. The test was conducted on the sandy loam soil (Munpo series), and its salt concentration was 0.42% at the time of planting. The emergence speed per kind of PE film mulching was in the order of black > coloration > transparent > green color, with the black color showing the fastest speed. The temperature change during a day per kind of PE film mulching was in the order of transparent > coloration > green > black color. As for the salt concentration in the soil for each different way of water management, the salt concentration in the treatment of drip irrigation with 1 week interval was lower than that drip irrigation with 2 weeks interval. As for the growth of above-aerial part, plant length was higher, number of tiller and leaves were more and dry weight of above-aerial part was larger in the treatment of drip irrigaton with 1 week interval than drip irrigation with 2 weeks interval. As for the yield of potatoes depending on were higher with pear color vinyl treatment. each way of water management, the yield in the treatment of drip irrigation with 1 week interval was more than drip irrigation with 2 weeks interval. The yield for each different kind of PE film mulching in the weekly drip-irrigation management section was in the order of transparent ≥ black ≥ coloration ≥ green color. In both of total phenol content and DPPH free radical activity experiments, the content and activity

Treatment of spontaneous retroperitoneal hemorrhage (SRH) is mainly conservative. However, if intra-abdominal hypertension (IAH) or abdominal compartment syndrome (ACS) develops, the treatment strategy should be more aggressive. Surgical decompression has been the gold standard; however, it is very invasive and highly morbid. Thus, percutaneous catheter drainage (PCD) has been introduced as an alternative therapeutic option. We report on a case of successful PCD for prevention of progression of IAH to ACS in a patient with SRH after coronary stent implantation. This case showed that PCD can be an efficacious and safe method for treatment of IAH with impending ACS.

This study conducted experiments on the reclaimed land of Saemangeum located in Jeongrabuk-do in order to gain basic information about growth characteristics and yield ability according to soil salinity. Having soil excluding salt as a control group, this study adjusted the specimens’ soil salinity to level 4 and then planted four varieties including Ilmichal Corn to investigate the growth or grain yield according to salinity. About the corn establishment rate according to soil salinity, over 97% up to 3.2 dS m-1 was established, and then, it was reduced gradually according to the increase of concentration. According to the salt concentration of soil more required growth duration from seeding to heading comparing to non-treatment salt was delayed, at 1.6 dS m-1, two days were delayed, at 3.2 dS m-1, four to six days were delayed differently by varieties, and at 4.8 dS m-1, six to 10 days were delayed. About the plant fresh weight according to soil salinity, Chalok 4 and Eolrukchal indicated 93%~97% or so compared with the salt-free one at 1.6 dS m-1, and Chalok No. 4 showed 79% at the salinity of 3.2 dS m-1, too, and it was a relatively higher growth percentage than those of the other varieties. In terms of dried seed weight according to soil salinity, compared with the corns cultivated in the control group, averagely 12.1% was lowered at the time of cultivation at 1.6 dS m-1, and 3.2 dS m-1 40% was lowered, and about 70% was lowered at 4.8 dS m-1. According to the result of examining the point of time that dried seed start to reduce due to soil salinity with the regression equation, soil salinity which starts the reduction of grain weight is 1.67 dS m-1~2.18 dS m-1, and it differs by varieties, and EC of 50% that the yield reduces in half is 2.96 dS m-1 ~4.45 dS m-1. And the degree of influence on each of the growth factors according to soil salinity is founded to be in the order of establishment rate<plant length=the ear’s fresh weight<the plant fresh weight<yield.

Polyethyleneglycol-adsorbed–superoxide dismutase (PEG-SOD), has been proposed as an effective agent for reducing free radical-mediated injury. The objective of this study was to investigate a protective effect of PEG-SOD supplementation on ovarian tissue during transplantation. Ovaries from F-1 mice were collected and vitrified. After warming, ovaries were autotransplanted under kidney capsule. Mice were randomly divided into four groups according to dose of PEG-SOD, (0 U/ml, 100 U/ml, 1,000 U/ml and 10,000 U/ml respectively). Grafted ovaries were retrieved 2, 7 and 21 days later. PEG-SOD was treated by intraperitoneal injection once every 48 hours and especially for 21 days group, after first week treatment, PEG-SOD was treated once every 4 days. Morphology of ovaries was assessed histological analysis and ELISA for FSH was performed to evaluate restoration of ovarian function. In 2 days groups, morphologically intact follicle ratio of 10,000 U/ml group was significantly higher than other groups. In 7 days groups, morphologically intact follicle ratio was significantly higher in all treatment groups. In 21 days groups, there was no significant difference of intact follicle ratio in total follicles in all groups but intact primordial, primary and secondary follicles ratio was higher in 10,000 U/ml group. FSH levels in blood serum were decrease as time goes on, but there is no statistical difference in each groups. In conclusion, the data of the present study show that PEG-SOD has a beneficial effect on preservation of the morphologically intact follicle.

Ovarian tissue cryopreservation and transplantation causes follicle depletion. To overcome this problem, we investigate the effect of Anti-Müllerian hormone (AMH), a follicle recruitment control hormone, supplementation before and/or after mouse ovarian transplantation. A total of 120 5-week-aged BD F-1 female mice were used. The mice were randomly divided into four groups according to AMH doses (0, 5, 25, 125 μg/mL, respectively). AMH was injected intraperitoneally on every other day for a week before, after, or before and after transplantation of ovaries under kidney capsules was performed. One week after transplantation, follicular normality was evaluated by histological analysis and TUNEL assay. In Group A and C, morphologically intact follicle (G1) ratios of AMH treated groups showed no statistically significant difference. In Group B, G1 ratios of 25 and 125 μg/mL of AMH treated groups were higher than those of 5 μg/mL treated group, but there was no improvement in G1 ratio after AMH treatment. In every group, apoptotic follicle ratios did not show any trend according to AMH treatment. Proportions of primordial follicle were not significantly different according to AMH treatment in all groups. The result of the present study demonstrated that AMH treatment during on transplantation of cryopreserved ovaries has no significant effect on follicle survival and prevention of follicle depletion.

Objective : To investigate the effects of Simvastatin and Methylprednisolone on ovarian tissue cryopreservation and transplantation using mouse models. Methods : The mice were randomly distributed into 1 control and 3 experimental groups. The B6D2F1 mice were given oral Simvastatin (5 mg/kg), intravenous Methylprednisolone (15 mg/kg), or a combination of both at 2 hours before ovariectomy. Same volume of normal saline was given perorally in the control group at 2 hours before ovariectomy. The ovarian tissues were vitrified accrording to our protocols. The vitrified ovaries were warmed 1 week later and auto-transplanted under bilateral kidney capsules. The ovaries and blood sera were collected at 2, 7 or 21 days after transplantation. Histological analysis, TUNEL assay, immuno-histochemistry for CD31, serum AMH level and embryonic development after in vitro fertilization were assessed for evaluation. Results : With regard to the total grade 1 follicle rate, both Simvastatin or Methylprednisolone treated groups were significantly increased at 2, 7 or 21 days after transplantation (except Simvastatin treated group at 7 days). A combination of Simvastatin and Methylprednisolone group was significantly improved in terms of the total G1 follicle rate, apoptotic follicle rate, CD31 positive area and serum AMH after ovarian tissue transplantation. However, there were no statistically difference with respect to the oocyte maturation rate, blastulation rate, and the other embryonic development parameters after in vitro fertilization procedure among the four groups. Conclusion : Our results suggest that combined donor Simvastatin and Methylprednisolone have beneficial effects on the quality and function of transplanted ovarian tissues.

Glucosinolates of Brassica rapa collection from Korea genebank were measured to determine total glucosinolate content and their variation of diverse glucosinolates; Around 100 accessions representing the different morphotypes and geographical origin of Brassica rapa were analysed. The principal component analysis was performed to evaluate the differences among morphotypes using the profiles of 14 glucosinolates identified from the leaves. DMRT test and box plots showed the significant difference between total glucosinolates of subspecies. Most of turnip accessions had higher gluconilates compared to the other type accessions, Chinese cabbage and pak choi. These accessions will be used for GWAS study for glucosinolate. Now they are being finger-printed by genotyping by sequencing (GBS). Among these accession, we selected a turnip accession with high amount of glucosinolate, K0466 and two Chinese cabbage accession with low amount of glucosinolate, K0015 and K0621. To analyse quantitative traits loci (QTL) for glucosinolate synthesis, these three accessions were fixed through microspore culture. Finally, six homozygous lines were selected and were crossed each other to make F1 hybrids. We just harvested F2 seeds and transferred doubled haploid plants to pots. QTL analysis for glucosinolate will be performed these F2 and DH population.

May-Thurner syndrome is caused by blockade of local venous flow due to local vascular intimal proliferation, caused by repeated pulsatile compression of the iliac or iliofemoral vein between the iliac artery and the lumbar spine. In this case, we confirmed May-Thurner syndrome using lower extremity computed tomographic angiography and venography. However, on venography, it was impossible to distinguish the left iliac vein from the collateral vein; a thrombus was also seen, although some of the thrombus was not seen clearly. These problems were overcome with use of intravascular ultrasound. We report on intravascular ultrasound guided treatment of May-Thurner syndrome.

May-Thurner syndrome is associated with deep vein thrombosis resulting from chronic compression of the iliac vein against the lumbar vertebrae caused by the overlying common iliac artery. Stent insertion into the compressed lesion is used in treatment of May-Thurner syndrome. Various complications can occur during angioplasty while using a stent. Among these complications, shrinkage of the vein below the stent, a rare complication, was observed in our hospital during treatment of a patient with May-Thurner syndrome. Different complications can occur when venous angioplasty is performed, unlike that when arterial angioplasty is performed.

본 연구에서는 새만금 지역에 조성된 신간척지 포장에서 담수와 하작물 재배처리가 제염 및 후작으로 재배한 청보리의 생육 및 수량에 미치는 영향을 조사 분석하여, 신간척지포장의 조기제염 기술을 개발하고자 하였다.1. 담수기간별 하작물 재배후 청보리 입모를 보면 무담수에서 m2당 216개로 입모율 25%, 1개월 담수 43%, 2개월과 3개월 담수 58%, 벼 재배구는 60% 이었다.2. 토양염농도는 청보리 파종 시에 무담수 처리에서 0.50%로 높았고, 그 외 담수처리에서는 0.2% 이내로 낮았으며, 생육 중기까지는 높아지다가 생육후기에는 파종시의 수준과 비슷하였다.3. 초장과 간장은 담수기간이 길수록 많았으며, 경수도에서 504%, 벼 재배에서는 536% 증수하였다.5. 사료가치는 담수 1개월은 단백질함량과 섬유소함량이낮게 나타났고, 담수 3개월은 벼 재배구와 사료가치면에서 비슷한 결과를 보였다.이상의 결과를 종합하여 볼 때, 새로 조성된 간척지(사양토)에서 제염을 목적으로 담수할 경우 3개월 이상 담수하거나 벼를 재배하여 제염을 하고 밭작물(청보리)을 재배하여야 어느 정도 수량을 얻을 수 있을 것으로 판단된다.많아지는 경향이었다.4. 수량은 무담수에서는 극히 적었으며, 담수기간이 길수록 수량은 급격히 증가하여, 무담수 대비 3개월 담수에서 504%, 벼 재배에서는 536% 증수하였다. 5. 사료가치는 담수 1개월은 단백질함량과 섬유소함량이낮게 나타났고, 담수 3개월은 벼 재배구와 사료가치면에서 비슷한 결과를 보였다.이상의 결과를 종합하여 볼 때, 새로 조성된 간척지(사양토)에서 제염을 목적으로 담수할 경우 3개월 이상 담수하거나 벼를 재배하여 제염을 하고 밭작물(청보리)을 재배하여야 어느 정도 수량을 얻을 수 있을 것으로 판단된다.

제염중인 간척지 토양에 감자재배 가능성을 검토코자 새만금 간척지 토양을 대조구를 포함한 4수준의 염농도로 조정하여 충진한 포트에 5개의 감자 품종을 식재하여 시험을수행하였다.1. 토양염농도에 따른 감자 입모는 4.8 dS m-1까지 결주없이 균일하게 입모 되었다. 염농도별 감자 출현소요일수는 숙전토양 비하여 1.6 dS m-1에서는 품종에 따라 3-4일이 지연되었고, 3.2 dS m-1에서는 6-10일이,4.8 dS m-1에서는 7-13일이 지연되었다.2. 토양염농도에 따른 경수는 숙전토양에 비해 1.6 dS m-1에서는 품종에 따라 각각 14%-58%가 감소되었으며,이후 토양염농도가 높아질수록 감소가 심했다. 초장은주당 경수가 적었을 때 커지는 경향으로 염농도보다경수의 영향을 더 크게 받았다.3. 괴경 수량은 대조구 대비 1.6 dS m-1일때 33.7%, 3.2 dS m-1일때 59.5%, 4.8 dS m-1에서는 79.3% 가 감소되었다.4. 회귀곡선을 이용하여 수량의 감소가 시작되는 염농도를 분석한 결과 추동은 1.2 dS m-1, 추백은 1.8 dS m-1,추강과 추영은 1.9 dS m-1, 그리고 수미는 2.0 dS m-1이었고, 수량의 50%가 감소되는 염농도는 추백은 2.4 dS m-1,추동 2.45 dS m-1, 추강 2.81 dS m-1, 추영 3.03 dS m-1,수미는 3.29 dS m-1였다.이상의 결과를 종합하였을 때 간척지 재배에 적당한 감자품종은 수미로 판단된다.

Human embryonic stem (ES) cells are a potential source of cells for developmental studies and for a variety of applications in transplantation therapies and drug discovery. However, human ES cells are difficult to culture and maintain at a large scale, which is one of the most serious obstacles in human ES cell research. Culture of human ES cells on MEF cells after disassociation with accutase has previously been demonstrated by other research groups. Here, we confirmed that human ES cells (H9) can maintain stem cell properties when the cells are passaged as single cells under a feeder-free culture condition. Accutase-dissociated human ES cells showed normal karyotype, stem cell marker expression, and morphology. We prepared frozen stocks during the culture period, thawed two of the human ES cell stocks, and analyzed the cells after culture with the same method. Although the cells revealed normal expression of stem cell marker genes, they had abnormal karyotypes. Therefore, we suggest that accutase-dissociated single cells can be usefully expanded in a feeder-free condition but chromosomal modification should be considered in the culture after freeze-thawing.

The objective of this study was to investigate the response of seed germination based on seed treatment of supercritical carbon dioxide (SC-CO2) sterilization. Seeds of eleven species were treated with SC-CO2 at 150 bar, 55℃ and 15 min (condition of fungi sterilization). Standard germination tests were used to evaluate germinability of seeds. Compared with non-treated seeds, SC-CO2 treatment caused significant variation of germination rate in different species. Different species showed different response to SC-CO2 treatment. The results suggest that crop of Leguminosae family is suitable for SC-CO2 sterilization.