The interaction of mastoparan B, a cationic tetradecapeptide amide isolated from the hornet Vespa basalis, with phospholipid bilayers was studied with synthetic mastoparan B and its analogue with Ala instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and its analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an α-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic face in the amphipathic α-helix of MP-B critically affected the biological activity and helical content.
1. 들깨는 용도면에서 볼 때 종실은 식용으로, 기름은 식용과 공업용, 깻잎은 채소로 또 깻잎의 정유성분은 향료로 이용될 수 있는 다용도 작물이다. 2. 종실의 단백질 함량은 18-28% 정도이고 단백질의 아미노산조성에 있어 글루타메이트(glutamate)함량은 계란보다 월등히 많다. 3. 종실의 기름 함량은 35~54%로서 기름중에는 리놀렌산(linolenic acid)이 60% 이상 함유되어 있으므로 생리적 기능성 식품으로 우수한 특성을 갖고 있으며 필수지방산의 공급원으로서도 중요하다. 4. 기름의 특성상 들기름은 고도의 불포화 지방산인 리놀렌산이 주성분이기 때문에 들기름을 짜서 상온에서 보관하게 되면 산패(oxidation)되기 쉬우므로 장기간 저장 이용하기 어렵다. 5. 깻잎은 비타민 C와 베타카로틴(β-carotene) 함량이 많고 다른 미량성분도 풍부하게 함유되어 있으므로 들깨잎은 생식용 채소로서의 이용가치가 높다. 6. 깻잎의 정유(essential oil) 성분은 페릴라알데하이드(perilla aldehyde)가 주성분이며 이는 식품이나 식품첨가제로 쓰일 때 광범위한 항균작용(antimicrobial activity)을 나타낸다. 7 들깨나 자소잎의 붉은 색소중에는 안토시아닌(anthocynins)이 다량 함유되어 있으며 이는 식품색소로 널리 쓰인다.
Lagrange 방법과 B-spline 기법을 이용한 경계요소법으로 경사면에 작용하는 고립파의 특성을 연구하였다. 최대 처오름높이는 실험자료와 비교한 결과, 기존의 경계요소법에 의한 결과에 비해 높은 정확도를 나타냈다. 또한, 벽면 경사각의 변화에 따른 최대 처오름높이와 속도벡터의 변화를 나타내었으며, 경사면에 작용하는 동수압의 특성을 조사하였다.
Activated carbon is amorphous and its intraparticle pores are well developed. Thus it has high adsorption capabilities and catalytic effect, and is utilized in many areas of industries such as food processing. In recent years, the demand of activated carbon has been increased to solve the environmental problems-waste treatment and removal of poisonous gas. Therefore, in this study an activated carbon was made using the waste and then the industrial characteristics of the produced activated carbon were investigated. The result showed that the carbonization yield was decreased when carbonization temperature was increased from 400 to 600℃ and that the optimal carbonization temperature was 500℃. The optimal concentration of NaOH for removing ash in the raw sample was 1~2N. The iodine adsorption of activated carbon using waste sample at 500℃ carbonization was 1204.28mg/g. The activation result of carbonization sample showed that the optimal activation condition was the carbonization at 500℃ and the activation at 800℃. So the production of activated carbon was thought to be possible, reused as resource, and decreased the environmental pollution.
The growth and heavy metal experiments revealed oppositive interactions between toxic metals(Zn and Cd) and Mn when the coastal diatom T. pseudonana were used. Cd and Zn inhibited the algal growth rate only at low Mn ion concentrations and this effect could be accounted for an inhibition of cellular Mn take by the toxic metals. Mn and Zn inhibited cellular Cd take and this indicated a reciprocal effects among the metals with respect to metal take. Saturation kinetics modeling of the take data was consistent with two metals competing with each other for binding to the Mn take system and with both Cd and Mn being transported into the cell by that system. Mathematical modeling of Mn and Cd take data revealed evidence for a Cd efflux system.