Flammulina velutipes, known as winter mushroom in the family of Physalacriaceae, is the main edible and export mushroom with the third highest production after oyster and king oyster mushroom in Korea. However, as normal consumers regard F. velutipes as a simple subsidiary material, there is a limitation to increasing mushroom demand. In order to overcome the consumption limit and increase the differentiation of new varieties, it is necessary to breed varieties with enhanced functionality in consideration of consumer preferences. Therefore, the study was performed to analyze nutrient components and several useful functional substances with 26 genetic resources of F. velutipes. Analyses of inorganic compound(Ca, K, Mg) and 15 amino acids revealed that Strain 4148 had the highest content among the 26 strains. Beta-glucan, which increases immune activity and polyphenol, which exert antioxidant effects were higher in non-white strains than in white strains with a small number of exceptions. Among the five fatty acids, linoleic acid(an omega-6 fatty acid) and α-linolenic acid(an omega-3 fatty acid), were detected in six mushroom strains. α-linolenic acid, which was not found in five major mushrooms including oyster mushrooms, was identified in F. velutipes. The results of HPLC analysis showed that ‘Auram’ (Strain 4232) and ‘Baekseung’(Strain 4230) had the highest content of the stabilizing neurotransmitter GABA(15.38 μg/ml and 20.56 μg/ml, respectively) among non-white and white strains, respectively. Our findings provide useful information for breeding F. velutipes to obtain strains with enhanced functionality.

Despite the long history of mushroom use, studies examining the genetic function of mushrooms and the development of new varieties via bio-molecular methods are significantly lacking compared to those examining other organisms. However, owing to recent developments, attempts have been made to use a novel gene-editing technique involving CRISPR/Cas9 technology and genetic scissors in mushroom studies. In particular, research is actively being conducted to utilize ribonucleoprotein particles (RNPs) that can be genetically edited with high efficiency without foreign gene insertion for ease of selection. However, RNPs are too large for Cas9 protein to pass through the cell membrane of the protoplasmic reticulum. Furthermore, guide RNA is unstable and can be easily decomposed, which remarkably affects gene editing efficiency. In this study, nanoparticles were used to mitigate the shortcomings of RNP-based gene editing techniques and to obtain transformants stably. We used Lentinula edodes (shiitake mushroom) Sanjo705-13 monokaryon strain, which has been successfully used in previous genome editing experiments. To identify a suitable osmotic buffer for the isolation of protoplast, 0.6 M and 1.2 M sucrose, mannitol, sorbitol, and KCl were treated, respectively. In addition, with various nanoparticle-forming materials, experiments were conducted to confirm genome editing efficiency via the formation of nanoparticles with calcium phosphate (CaP), which can be bound to Cas9 protein without any additional amino acid modification. RNPs/NP complex was successfully formed and protected nuclease activity with nucleotide sequence specificity.

The purpose of this study was to breed a variety of stable productivity and high storage characteristics of white Hypsizygus marmoreus, which has high demand at domestic and global market due to a low bitter taste and the preference for white species. Accordingly, ‘Baekmaru’ was bred by hybridizing brown and white cap color species and backcrossing with white species. The ‘Baekmaru’ variety was bred by crossbreeding the brown and white species and backcrossing the white species. Through repeated cultivation of ‘Baekmaru’, a variety with a low contamination rate was selected when culturing the spawn for stable cultivation. As a results of demonstration test, the yield of ‘Baekmaru’ was 14% higher than that of the commercial variety. In addition, among the fruiting body characteristics of ‘Baekmaru’, diameter and thickness of the pileus were 16.43±15.27mm and 6.46±0.58mm, which were slightly higher than the commercial variety, and the hardness was 2.69±0.89N for the pileus and 3.09±0.89N for the stipe. The shelf life showed less change in thickness and color of pileus compared to commercial variety. The hardness of pileus of 'Baekmaru' was maintained in the range of 3.5 to 4.0N in the 4oC and 4oC and 20oC mixed treatment until 30 days of storage, and was higher than that of the commercial variety. Therefore, it was suggested to be excellent in storability.

As the importance of public health increases with the spread of infectious diseases, functionality has become a factor affecting consumers’ purchase of mushrooms. Therefore, the bioactive components of button mushrooms (Agaricus bisporus), which are generally known to promote button mushroom consumption, were analyzed. White and brown button mushrooms were compared and white beech mushroom (Hypsizygus marmoreus) were used as a control. White button mushrooms had higher sugar and inorganic potassium concentrations than brown button mushrooms, whereas sodium, magnesium, and vitamin C concentrations were not significantly different between the different button mushrooms. Moreover, there was approximately twice as much ergosterol in white button mushrooms than brown button mushrooms. Brown button mushrooms had higher concentrations of β-glucan and oxalic acid than white button mushrooms, but there was no significant difference in total organic acid content between the two mushroom types. High concentrations of the essential amino acids, ergothioneine, isoleucine, and leucine and the non-essential amino acids, glycine and alanine, were observed. Concentrations of the vitamin B group and total polyphenols were also high.

In the flux used in the batch galvanizing process, the effect of the component ratio of NH₄Cl to ZnCl₂ on the microstructure, coating adhesion, and corrosion resistance of Zn-Mg-Al ternary alloy-coated steel is evaluated. Many defects such as cracks and bare spots are formed inside the Zn-Mg-Al coating layer during treatment with the flux composition generally used for Zn coating. Deterioration of the coating property is due to the formation of AlClx mixture generated by the reaction of Al element and chloride in the flux. The coatability of the Zn-Mg-Al alloy coating is improved by increasing the content of ZnCl2 in the flux to reduce the amount of chlorine reacting with Al while maintaining the flux effect and the coating adhesion is improved as the component ratio of NH4Cl to ZnCl2 decreases. Zn-Mg-Al alloy-coated steel products treated with the optimized flux composition of NH₄Cl•3ZnCl₂ show superior corrosion resistance compared to Zn-coated steel products, even with a coating weight of 60 %.

국내의 주요 산느타리 품종인 호산47(일핵, 산타리 배우자), GB19(일핵, 산타리 배우자), 호산, 여름느타리1호, 삼복, 강산, 약산, 자산, 향산, 여름느타리2호의 유전체를 Hiseq을 이용하여 해독하였고 이 서열 정보에서 SSR을 분리하여 특성구명을 하였다. 일핵균사인 호산 47, GB19 의 유전체의 크기는 각각 37.3와 37.2 Mbp이고, 이핵균사인 나머지 산느타리 품종의 유전체 크기는 47.1~61.1 Mbp인 것으로 밝혀졌다. 품종별 총 SSR의 수는 HS47이 711개로 가장 적고, 강산이(GS)이 1.5배 많은 1,106개로 최다를 기록하였다. SSR의 repeat motif 중에서 hexanucleotide 와 octanucleotide가 가장 많은 빈도로 관찰되었고, 가장 많이 관찰되는 반복서열은 CGA/TCG, A/T, CTC/GAG이었다. SSR의 길이는 모든 품종에서 변이가 많아 유용성이 높은 20~30 nt가 가장 높은 비중인 70%를 차지하였다.

팽이(Flammulina velutipes)는 주름버섯목(Agaricales), 뽕나무버섯과(Physalacriaceae)에 속하는 담자균류로서, 국내 생산량은 연간 31,818톤(‘19)으로 전체 생산량의 약 18%이며 버섯 전체 수출액의 약 36%를 차지할 정도로 대표적인 식용버섯이다. 그러나 국내 재배되고 있는 팽이 국산품종의 비중이 31.6%(20´)로 매우 낮으므로 국제 경쟁력이 우수한 국내육성 품종확보가 필요하며 수출 버섯으로서 보다 품질이 우수한 품종 육성이 필요하다. 이에 본 연구는 농진청에서 육성한 ‘백승’과 ‘우리1호’의 단핵 균주를 확보하여 mon-mon교잡을 통해 ‘설한’ 품종을 육 성하였다. 균사배양 특성은 25 ̊C에서 균사생장이 가장 좋았으며 20, 25 ̊C에서 대조품종보다 균사 생장속도가 빨랐다. 병재배 시 배양 최적온도는 15~17 ̊C로 배지온도가 20 ̊C이상 되지 않게 관리해야하며 생육온도는 7~8 ̊C로 대조품종에 비해 다소 높다. 또한 자실체 수량은 병당 (850 mL) 226.5±8.0 g으로 대조품종에 비해 18.4% 증수 하였으며 초발이일수가 2일 빨라 전체적인 생육기간이 단축되었다. 또한 설한의 외형은 대조품종보다 갓이 작고 대는 굵었으며 갓 색이 더 밝았다. 대치배양과 유전적 다형성 검정 결과, 설한이 다른 흰색 품종들과 유전적으로 구별되는 것을 확인하였다.