온실가스 배출량을 최소화하기 위하여 가열 없이 생산이 가능한 상온 아스팔트 포장 공법도 2000년 초부터 개발되어 활용되고 있으 나, 기술적 한계로 인해 성능 확보가 어려워 대부분 기층용으로 활용중에 있다. 상온 아스팔트 혼합물은 유화아스팔트를 사용하는데 양생하는 동안 혼합물 내부에 있는 물이 증가됨에 따라 혼합물 내부의 높은 공극률이 발생하게 되어 포장의 성능을 확보하는데 한계 가 있다. 따라서 본 연구에서는 유화 아스팔트 내 아스팔트 고형분 함량을 증가시켜 물 함량을 최소화함으로서, 양생시간을 단축하고 낮은 공극률 확보를 통한 상온 아스팔트 혼합물의 성능의 변화를 평가하였다. 시험결과, 고형분 함량이 변화에 따라 공극률 및 간접인 장강도, 터프니스 물성이 변화가 나타났다. 하지만 고함량 고형분의 유화 아스팔트를 상온 아스팔트 혼합물에 적용하기 위해서는 최적 함수비 결정방식 및 양생방식 등에 대한 추가적인 연구가 필요한 것으로 나타났다.

본 연구에서는 온도 반응형 발열 아스팔트 포장 공법 개발을 위하여 온도 반응형 신소재의 아스팔트 적용 방안과 이를 아스팔트 재 료에 적용하기 위한 기초 연구를 수행하였다. 발열 아스팔트 포장에 적합한 상변화 온도 범위에 따른 PCM 재료 선정 및 아스팔트 재 료에 적용하기 위한 캡슐화 방안을 검토하고 다양한 소재를 활용한 캡슐화된 PCM 신소재(ePCM)를 제작하였다. 이에 대한 발열 특성 및 물리적 특성 평가를 수행하였다.

최근 결빙으로 인한 교통사고가 빈번히 발생하고 있으며, 도로순찰시 육안 인식이 어려운 도로살얼음 검지를 위해 다양한 방식의 검지센서가 도입되고 있다. 본 연구에서는 국내외 상용화되어 있는 차량부착식 노면상태 검지센서에 대한 현장 검증을 통해 국내 도 로조건에의 적용 가능성을 검토하였다. 차량부착식 검지센서의 성능을 평가하기 위해 한국건설기술연구원의 연천SOC실증연구센터 내 의 도로기상재현 실험시설에 결빙(Ice), 습윤(Wet), 건조(Dry) 등 3가지의 노면상태가 육안으로 명확히 구분이 가능하도록 도로환경을 구현하였으며, 센서종류별로 차량에 부착하여 다양한 도로상태를 측정하였다. 평가결과 노면상태 측정결과의 정확도는 높은 것으로 나 타났으나, 그 외의 측정항목의 정확도는 상당한 차이가 발생하기도 하였다. 향후 다양한 도로환경 조건에서 추가적인 시험을 통해 차 량부착식 노면상태 검지센서의 현장적용을 기반자료로 활용할 수 있을 것으로 판단된다.

최근 급격한 기후 변화로 인해 도로 교통사고의 발생 빈도가 증가하고 있으며, 특히 겨울철에 자주 발생하는 도로 살얼음(블랙아이 스) 현상이 주요 원인 중 하나로 지목되고 있다. 도로살얼음의 형성 메커니즘은 다양한 요인에 따라 복합적으로 작용하며, 당시의 도 로 기상 조건과 도로의 기하학적 구조에 따라 얼음의 형태 및 강도가 결정된다. 그중에서도 도로 노면 온도는 도로살얼음 형성에 중 요한 요소로, 여러 나라에서 겨울철 교통안전 평가를 위한 주요 지표로 사용되고 있다. 그러나 현재 도로 노면 온도에 대한 명확한 정 의가 부족할 뿐만 아니라, 측정 방법에 따라 계측 편차와 온도 손실 등 여러 한계가 존재해 정확한 온도 측정이 어려운 실정이다. 이 에 본 연구는 지중 깊이에 따른 온도 데이터와 도로 기상 데이터를 결합하여 보다 정밀한 도로 노면 온도 예측 방법을 제시하는 것을 목적으로 한다. 연구를 위해 지중 깊이 2cm, 3cm, 4cm, 5cm, 7cm, 9cm, 15cm, 20cm에 각각 온도 센서를 설치하였으며, 기상 데이터는 해당 지점에서 2m 떨어진 AWS(Automatic Weather System)를 통해 대기 온도, 습도, 강수량, 일사량 등의 정보를 수집하였다. 이를 바 탕으로 지중 온도와 기상 조건의 상관관계를 활용하여 노면 온도를 예측하는 방법론을 도출하였다. 본 연구의 결과는 도로 노면 온도 예측의 정확성을 향상시킬 뿐만 아니라, 새로운 접근 방식을 통해 노면 온도의 정의를 재정립하는 데 기여할 것으로 기대된다.

Sucralose는 식품 분야에서 sucrose 대체물로 사용되며 세계적으로 승인된 무열량 고강도 인 공 감미료이다. 하지만 감미료가 뇌에 미치는 영향에 대한 조사는 미비하여 본 연구에서 scopolamine으 로 유발된 기억력 손상 동물모델에서 sucralose 단기 섭취가 인지 및 기억 보호 효과를 가지는지 확인하 였다. Sucralose 2, 5, 10 mg/kg를 경구투여하였으며 30분 후 대조군, 약물군에 scopolamine (1mg/kg) 복강투여를 진행하였고 정상군에는 식염수로 복강투여 후 행동실험을 수행하였다. 결과적으로 Y-maze, passive avoidance, Morris water maze에서 대조군과 비교해 10% 이상의 인지기능이 회복되는 결과를 도출하였다. 또한, 전염증성 사이토카인을 측정한 결과 sucralose가 IL-6와 TNF-α를 30% 이상 억제 하는 것을 보였으며 세포 내 신호 메커니즘으로 ERK-CREB의 발현량을 관찰한 결과 농도 의존적으로 증가하였다. 따라서 sucralose는 건망증 환자의 예방과 치료를 위한 기능성 식품과 관련이 있다고 시사 된다.

PURPOSES : In this study, the basis for improving the maintenance method of road pavement in Jeju Island, where deterioration is accelerating, was presented through field construction and analysis of various combinations of maintenance methods. METHODS : Construction was performed on Jeju Island's Aejo Road, which has high traffic and frequent early damage, using various asphalt mixtures mainly applied in Jeju Island, with different maintenance cross-sections depending on the level of repair. The quality and performance of the asphalt mixture collected during construction were evaluated, and MEPDG was used to analyze the service life according to the type and maintenance level of the mixture. RESULTS : While the mixture for the surface layer satisfied the quality standards and had excellent rutting and moisture resistance performance, the asphalt mixture for the intermediate and base layer did not satisfy the quality standards such as air voids, so it was judged that quality control was necessary during production. The section repaired to the base layer was found to be advantageous for the integrated behavior of the pavement and had the best structural integrity. As a result of predicting the service life, the estimated life of the section where only the surface layer was repaired was analyzed to be approximately 7 years, the section where the intermediate layer was repaired was 14.5 years, and the section where the entire section up to the base layer was repaired was analyzed to be 18 years. CONCLUSIONS : In Jeju Island, where deterioration is accelerating, it was analyzed that when establishing a maintenance plan, it is necessary to consider repairing the middle and base floors in order to secure the designed life of 10 years.

PURPOSES : There has been increasing interest in South Korea on warm-mix asphalt (WMA) and cold-mix asphalt (CMA) technologies that allow production of asphalt pavement mixtures at comparatively lower temperatures than those of hot-mix asphalt (HMA) for use in pavement engineering. This study aims to evaluate the feasibility of replacing HMA pavement with WMA pavement with the goal of reducing CO2 emissions associated with asphalt production for road construction. METHODS : Changes in the dynamic modulus characteristics of WMA and HMA according to short-term and long-term aging were evaluated. In addition, the effects of water damage were evaluated for short- and long-term aging stages. RESULTS : For WMA, in the process of mixing and short-term aging, early-age dynamic modulus decreased owing to low temperature and reduced short-term aging (STA) time. This could result in early damage to the asphalt pavement depending on the applied traffic load and environmental load. CONCLUSIONS : Mastercurves of the dynamic modulus were used for comparative analysis of WMA and HMA. Compared to the dynamic modulus after STA of HMA, the estimated aging time determined by experiments for WMA to achieve the required stiffness was more than 48 hours, which is equiva-lent to approximately 4 to 5 years real service life when converted. It is considered that further studies are needed for performance optimization to achieve early-age performance of the asphalt mixes.

PURPOSES : The purpose of this study was to evaluate the performance of a titanium dioxide (TiO2) asphalt surface treatment agent for reducing NOx on the roadside at laboratory and full scales. METHODS : To verify the NOx reduction performance of TiO2 and silicon-based resin-applied surface treatment agents at the lab scale, a bed flow photo reactor test (ISO standard) and a mixed tank photo reactor test designed to apply real-scale construction materials were conducted. Subsequently, the full-scale NOx reduction performance was verified using a full-scale demonstration facility, and the field construction capability of the TiO2 asphalt surface treatment agent was verified through actual road site application. RESULTS : The bed flow photoreactor and mixed tank photoreactor methods showed the same trend in the NOx removal performance. Evaluation of the NOx removal performance of the TiO2 surface treatment agent revealed that the NO removal rate was approximately 13% at the laboratory scale and 15% at full scale. CONCLUSIONS : Through this study, it was determined that the asphalt surface treatment agent applied with TiO2 will have a sufficient NOx reduction effect in an actual road site. In the future, it will be necessary to analyze the continuity of the effect according to traffic volume through continuous monitoring in the field.

Recently, with the increasing global demand for Ganoderma lucidum as a material for functional beverages, varieties with enhanced functionality are needed. As part of this effort, 10 strains were selected from a collection of 160 G. lucidum strains, based on their quantity and fruiting body characteristics. These included four strains collected in Korea, three in Japan, and three of unknown origin. Approximately 44% of the 1,290 hybrid strains were secured. After two rounds of bag cultivation in oak sawdust, three strains (G-20-4, G-20-5, and G-20-7) were ultimately selected for single-timbered cultivation. Among these, G-20-4 demonstrated the earliest harvesting with a large size and a bright cap. Additionally, this strain exhibited the highest levels of total glucan, β-glucan, total triterpenoid, total polyphenol, and total flavonoid content.

Urban areas in watersheds increase the impervious surface, and agricultural areas deteriorate the water quality of rivers due to the use of fertilizers. As such, anthropogenic land use affects the type, intensity and quantity of land use and is closely related to the amount of substances and nutrients discharged to nearby streams. Riparian vegetation reduce the concentration of pollutants entering the watershed and mitigate the negative impacts of land use on rivers. This study analyzes the data through correlation analysis and regression analysis through point data measured twice a year in spring and autumn in 21 selected damaged tributary rivers within the Han River area, and then uses a structural equation model to determine the area land use. In the negative impact on water quality, the mitigation effect of riparian vegetation was estimated. As a result of the correlation analysis, the correlation between the agricultural area and water quality was stronger than that of the urban area, and the area ratio of riparian vegetation showed a negative correlation with water quality. As a result of the regression analysis, it was found that agricultural areas had a negative effect on water quality in all models, but the results were not statistically significant in the case of urban areas. As a result of the model estimated through the structural equation, BOD, COD, TN, and TP showed a mitigation effect due to the accumulation effect of river water quality through riparian vegetation in agricultural areas, but the effect of riparian vegetation through riparian vegetation was found in urban areas. There was no These results were interpreted as having a fairly low distribution rate in urban areas, and in the case of the study area, there was no impact due to riparian forests due to the form of scattered and distributed settlements rather than high-density urbanized areas. The results of this study were judged to be unreasonable to generalize by analyzing the rivers where most of the agricultural areas are distributed, and a follow-up to establish a structural equation model by expanding the watershed variables in urban areas and encompassing the variables of various factors affecting water quality research is required.

Flammulina velutipes, known as winter mushroom in the family of Physalacriaceae, is the main edible and export mushroom with the third highest production after oyster and king oyster mushroom in Korea. However, as normal consumers regard F. velutipes as a simple subsidiary material, there is a limitation to increasing mushroom demand. In order to overcome the consumption limit and increase the differentiation of new varieties, it is necessary to breed varieties with enhanced functionality in consideration of consumer preferences. Therefore, the study was performed to analyze nutrient components and several useful functional substances with 26 genetic resources of F. velutipes. Analyses of inorganic compound(Ca, K, Mg) and 15 amino acids revealed that Strain 4148 had the highest content among the 26 strains. Beta-glucan, which increases immune activity and polyphenol, which exert antioxidant effects were higher in non-white strains than in white strains with a small number of exceptions. Among the five fatty acids, linoleic acid(an omega-6 fatty acid) and α-linolenic acid(an omega-3 fatty acid), were detected in six mushroom strains. α-linolenic acid, which was not found in five major mushrooms including oyster mushrooms, was identified in F. velutipes. The results of HPLC analysis showed that ‘Auram’ (Strain 4232) and ‘Baekseung’(Strain 4230) had the highest content of the stabilizing neurotransmitter GABA(15.38 μg/ml and 20.56 μg/ml, respectively) among non-white and white strains, respectively. Our findings provide useful information for breeding F. velutipes to obtain strains with enhanced functionality.

PURPOSES : In this study, to improve the quality and construction performance of backfill materials for road excavation and restoration, the basic properties of rapid-hardening materials and stone sludge are analyzed to propose an optimal mix design. METHODS : To utilize rapid-hardening materials and stone sludge as controlled low-strength materials for pipeline construction in downtown areas, specimens were prepared for each compounding condition of fast-hardening materials. Flow, slump, and compressive strength tests were performed at various setting times. Subsequently, the physical and mechanical characteristics of the rapid-hardening backfill material for each mixing factor were analyzed. RESULTS : When ultrafast hardening cement and stone sludge are used, a setting time exceeding 30 min is required for a water–binder (W/B) ratio of 200% or higher. Considering the economic feasibility of ultrafast hardening cement, a W/B of 300% is considered the most suitable when high-performance superplasticizer and retarders are mixed. A flow test was performed on the rapid-hardening backfill material and the results show that if the mixing time exceeds 10 min, then the fluidity decreases rapidly, which necessitates a higher amount of superplasticizer. When ultrafast hardening cement is used, the initial strength (based on 4 h) is 0.7 MPa or higher for all formulations at a W/B ratio of 300%, and the compressive strength decreases slightly as the amount of superplasticizer is increased by 0.1%. CONCLUSIONS : Based on the fluidity and strength of the backfill material, which is composed of a rapid-hardening material and stone sludge, the most optimal performance is achieved when ultrafast-hardening cement with a W/B ratio of 300% is used in addition to a highperformance fluidizing agent 0.3% (wt./B) and retarder 0.2% (wt./B).

Despite the long history of mushroom use, studies examining the genetic function of mushrooms and the development of new varieties via bio-molecular methods are significantly lacking compared to those examining other organisms. However, owing to recent developments, attempts have been made to use a novel gene-editing technique involving CRISPR/Cas9 technology and genetic scissors in mushroom studies. In particular, research is actively being conducted to utilize ribonucleoprotein particles (RNPs) that can be genetically edited with high efficiency without foreign gene insertion for ease of selection. However, RNPs are too large for Cas9 protein to pass through the cell membrane of the protoplasmic reticulum. Furthermore, guide RNA is unstable and can be easily decomposed, which remarkably affects gene editing efficiency. In this study, nanoparticles were used to mitigate the shortcomings of RNP-based gene editing techniques and to obtain transformants stably. We used Lentinula edodes (shiitake mushroom) Sanjo705-13 monokaryon strain, which has been successfully used in previous genome editing experiments. To identify a suitable osmotic buffer for the isolation of protoplast, 0.6 M and 1.2 M sucrose, mannitol, sorbitol, and KCl were treated, respectively. In addition, with various nanoparticle-forming materials, experiments were conducted to confirm genome editing efficiency via the formation of nanoparticles with calcium phosphate (CaP), which can be bound to Cas9 protein without any additional amino acid modification. RNPs/NP complex was successfully formed and protected nuclease activity with nucleotide sequence specificity.