Sucralose는 식품 분야에서 sucrose 대체물로 사용되며 세계적으로 승인된 무열량 고강도 인 공 감미료이다. 하지만 감미료가 뇌에 미치는 영향에 대한 조사는 미비하여 본 연구에서 scopolamine으 로 유발된 기억력 손상 동물모델에서 sucralose 단기 섭취가 인지 및 기억 보호 효과를 가지는지 확인하 였다. Sucralose 2, 5, 10 mg/kg를 경구투여하였으며 30분 후 대조군, 약물군에 scopolamine (1mg/kg) 복강투여를 진행하였고 정상군에는 식염수로 복강투여 후 행동실험을 수행하였다. 결과적으로 Y-maze, passive avoidance, Morris water maze에서 대조군과 비교해 10% 이상의 인지기능이 회복되는 결과를 도출하였다. 또한, 전염증성 사이토카인을 측정한 결과 sucralose가 IL-6와 TNF-α를 30% 이상 억제 하는 것을 보였으며 세포 내 신호 메커니즘으로 ERK-CREB의 발현량을 관찰한 결과 농도 의존적으로 증가하였다. 따라서 sucralose는 건망증 환자의 예방과 치료를 위한 기능성 식품과 관련이 있다고 시사 된다.

PURPOSES : In this study, the basis for improving the maintenance method of road pavement in Jeju Island, where deterioration is accelerating, was presented through field construction and analysis of various combinations of maintenance methods. METHODS : Construction was performed on Jeju Island's Aejo Road, which has high traffic and frequent early damage, using various asphalt mixtures mainly applied in Jeju Island, with different maintenance cross-sections depending on the level of repair. The quality and performance of the asphalt mixture collected during construction were evaluated, and MEPDG was used to analyze the service life according to the type and maintenance level of the mixture. RESULTS : While the mixture for the surface layer satisfied the quality standards and had excellent rutting and moisture resistance performance, the asphalt mixture for the intermediate and base layer did not satisfy the quality standards such as air voids, so it was judged that quality control was necessary during production. The section repaired to the base layer was found to be advantageous for the integrated behavior of the pavement and had the best structural integrity. As a result of predicting the service life, the estimated life of the section where only the surface layer was repaired was analyzed to be approximately 7 years, the section where the intermediate layer was repaired was 14.5 years, and the section where the entire section up to the base layer was repaired was analyzed to be 18 years. CONCLUSIONS : In Jeju Island, where deterioration is accelerating, it was analyzed that when establishing a maintenance plan, it is necessary to consider repairing the middle and base floors in order to secure the designed life of 10 years.

PURPOSES : There has been increasing interest in South Korea on warm-mix asphalt (WMA) and cold-mix asphalt (CMA) technologies that allow production of asphalt pavement mixtures at comparatively lower temperatures than those of hot-mix asphalt (HMA) for use in pavement engineering. This study aims to evaluate the feasibility of replacing HMA pavement with WMA pavement with the goal of reducing CO2 emissions associated with asphalt production for road construction. METHODS : Changes in the dynamic modulus characteristics of WMA and HMA according to short-term and long-term aging were evaluated. In addition, the effects of water damage were evaluated for short- and long-term aging stages. RESULTS : For WMA, in the process of mixing and short-term aging, early-age dynamic modulus decreased owing to low temperature and reduced short-term aging (STA) time. This could result in early damage to the asphalt pavement depending on the applied traffic load and environmental load. CONCLUSIONS : Mastercurves of the dynamic modulus were used for comparative analysis of WMA and HMA. Compared to the dynamic modulus after STA of HMA, the estimated aging time determined by experiments for WMA to achieve the required stiffness was more than 48 hours, which is equiva-lent to approximately 4 to 5 years real service life when converted. It is considered that further studies are needed for performance optimization to achieve early-age performance of the asphalt mixes.

PURPOSES : The purpose of this study was to evaluate the performance of a titanium dioxide (TiO2) asphalt surface treatment agent for reducing NOx on the roadside at laboratory and full scales. METHODS : To verify the NOx reduction performance of TiO2 and silicon-based resin-applied surface treatment agents at the lab scale, a bed flow photo reactor test (ISO standard) and a mixed tank photo reactor test designed to apply real-scale construction materials were conducted. Subsequently, the full-scale NOx reduction performance was verified using a full-scale demonstration facility, and the field construction capability of the TiO2 asphalt surface treatment agent was verified through actual road site application. RESULTS : The bed flow photoreactor and mixed tank photoreactor methods showed the same trend in the NOx removal performance. Evaluation of the NOx removal performance of the TiO2 surface treatment agent revealed that the NO removal rate was approximately 13% at the laboratory scale and 15% at full scale. CONCLUSIONS : Through this study, it was determined that the asphalt surface treatment agent applied with TiO2 will have a sufficient NOx reduction effect in an actual road site. In the future, it will be necessary to analyze the continuity of the effect according to traffic volume through continuous monitoring in the field.

Recently, with the increasing global demand for Ganoderma lucidum as a material for functional beverages, varieties with enhanced functionality are needed. As part of this effort, 10 strains were selected from a collection of 160 G. lucidum strains, based on their quantity and fruiting body characteristics. These included four strains collected in Korea, three in Japan, and three of unknown origin. Approximately 44% of the 1,290 hybrid strains were secured. After two rounds of bag cultivation in oak sawdust, three strains (G-20-4, G-20-5, and G-20-7) were ultimately selected for single-timbered cultivation. Among these, G-20-4 demonstrated the earliest harvesting with a large size and a bright cap. Additionally, this strain exhibited the highest levels of total glucan, β-glucan, total triterpenoid, total polyphenol, and total flavonoid content.

Urban areas in watersheds increase the impervious surface, and agricultural areas deteriorate the water quality of rivers due to the use of fertilizers. As such, anthropogenic land use affects the type, intensity and quantity of land use and is closely related to the amount of substances and nutrients discharged to nearby streams. Riparian vegetation reduce the concentration of pollutants entering the watershed and mitigate the negative impacts of land use on rivers. This study analyzes the data through correlation analysis and regression analysis through point data measured twice a year in spring and autumn in 21 selected damaged tributary rivers within the Han River area, and then uses a structural equation model to determine the area land use. In the negative impact on water quality, the mitigation effect of riparian vegetation was estimated. As a result of the correlation analysis, the correlation between the agricultural area and water quality was stronger than that of the urban area, and the area ratio of riparian vegetation showed a negative correlation with water quality. As a result of the regression analysis, it was found that agricultural areas had a negative effect on water quality in all models, but the results were not statistically significant in the case of urban areas. As a result of the model estimated through the structural equation, BOD, COD, TN, and TP showed a mitigation effect due to the accumulation effect of river water quality through riparian vegetation in agricultural areas, but the effect of riparian vegetation through riparian vegetation was found in urban areas. There was no These results were interpreted as having a fairly low distribution rate in urban areas, and in the case of the study area, there was no impact due to riparian forests due to the form of scattered and distributed settlements rather than high-density urbanized areas. The results of this study were judged to be unreasonable to generalize by analyzing the rivers where most of the agricultural areas are distributed, and a follow-up to establish a structural equation model by expanding the watershed variables in urban areas and encompassing the variables of various factors affecting water quality research is required.

Flammulina velutipes, known as winter mushroom in the family of Physalacriaceae, is the main edible and export mushroom with the third highest production after oyster and king oyster mushroom in Korea. However, as normal consumers regard F. velutipes as a simple subsidiary material, there is a limitation to increasing mushroom demand. In order to overcome the consumption limit and increase the differentiation of new varieties, it is necessary to breed varieties with enhanced functionality in consideration of consumer preferences. Therefore, the study was performed to analyze nutrient components and several useful functional substances with 26 genetic resources of F. velutipes. Analyses of inorganic compound(Ca, K, Mg) and 15 amino acids revealed that Strain 4148 had the highest content among the 26 strains. Beta-glucan, which increases immune activity and polyphenol, which exert antioxidant effects were higher in non-white strains than in white strains with a small number of exceptions. Among the five fatty acids, linoleic acid(an omega-6 fatty acid) and α-linolenic acid(an omega-3 fatty acid), were detected in six mushroom strains. α-linolenic acid, which was not found in five major mushrooms including oyster mushrooms, was identified in F. velutipes. The results of HPLC analysis showed that ‘Auram’ (Strain 4232) and ‘Baekseung’(Strain 4230) had the highest content of the stabilizing neurotransmitter GABA(15.38 μg/ml and 20.56 μg/ml, respectively) among non-white and white strains, respectively. Our findings provide useful information for breeding F. velutipes to obtain strains with enhanced functionality.

PURPOSES : In this study, to improve the quality and construction performance of backfill materials for road excavation and restoration, the basic properties of rapid-hardening materials and stone sludge are analyzed to propose an optimal mix design. METHODS : To utilize rapid-hardening materials and stone sludge as controlled low-strength materials for pipeline construction in downtown areas, specimens were prepared for each compounding condition of fast-hardening materials. Flow, slump, and compressive strength tests were performed at various setting times. Subsequently, the physical and mechanical characteristics of the rapid-hardening backfill material for each mixing factor were analyzed. RESULTS : When ultrafast hardening cement and stone sludge are used, a setting time exceeding 30 min is required for a water–binder (W/B) ratio of 200% or higher. Considering the economic feasibility of ultrafast hardening cement, a W/B of 300% is considered the most suitable when high-performance superplasticizer and retarders are mixed. A flow test was performed on the rapid-hardening backfill material and the results show that if the mixing time exceeds 10 min, then the fluidity decreases rapidly, which necessitates a higher amount of superplasticizer. When ultrafast hardening cement is used, the initial strength (based on 4 h) is 0.7 MPa or higher for all formulations at a W/B ratio of 300%, and the compressive strength decreases slightly as the amount of superplasticizer is increased by 0.1%. CONCLUSIONS : Based on the fluidity and strength of the backfill material, which is composed of a rapid-hardening material and stone sludge, the most optimal performance is achieved when ultrafast-hardening cement with a W/B ratio of 300% is used in addition to a highperformance fluidizing agent 0.3% (wt./B) and retarder 0.2% (wt./B).

Despite the long history of mushroom use, studies examining the genetic function of mushrooms and the development of new varieties via bio-molecular methods are significantly lacking compared to those examining other organisms. However, owing to recent developments, attempts have been made to use a novel gene-editing technique involving CRISPR/Cas9 technology and genetic scissors in mushroom studies. In particular, research is actively being conducted to utilize ribonucleoprotein particles (RNPs) that can be genetically edited with high efficiency without foreign gene insertion for ease of selection. However, RNPs are too large for Cas9 protein to pass through the cell membrane of the protoplasmic reticulum. Furthermore, guide RNA is unstable and can be easily decomposed, which remarkably affects gene editing efficiency. In this study, nanoparticles were used to mitigate the shortcomings of RNP-based gene editing techniques and to obtain transformants stably. We used Lentinula edodes (shiitake mushroom) Sanjo705-13 monokaryon strain, which has been successfully used in previous genome editing experiments. To identify a suitable osmotic buffer for the isolation of protoplast, 0.6 M and 1.2 M sucrose, mannitol, sorbitol, and KCl were treated, respectively. In addition, with various nanoparticle-forming materials, experiments were conducted to confirm genome editing efficiency via the formation of nanoparticles with calcium phosphate (CaP), which can be bound to Cas9 protein without any additional amino acid modification. RNPs/NP complex was successfully formed and protected nuclease activity with nucleotide sequence specificity.

The purpose of this study was to breed a variety of stable productivity and high storage characteristics of white Hypsizygus marmoreus, which has high demand at domestic and global market due to a low bitter taste and the preference for white species. Accordingly, ‘Baekmaru’ was bred by hybridizing brown and white cap color species and backcrossing with white species. The ‘Baekmaru’ variety was bred by crossbreeding the brown and white species and backcrossing the white species. Through repeated cultivation of ‘Baekmaru’, a variety with a low contamination rate was selected when culturing the spawn for stable cultivation. As a results of demonstration test, the yield of ‘Baekmaru’ was 14% higher than that of the commercial variety. In addition, among the fruiting body characteristics of ‘Baekmaru’, diameter and thickness of the pileus were 16.43±15.27mm and 6.46±0.58mm, which were slightly higher than the commercial variety, and the hardness was 2.69±0.89N for the pileus and 3.09±0.89N for the stipe. The shelf life showed less change in thickness and color of pileus compared to commercial variety. The hardness of pileus of 'Baekmaru' was maintained in the range of 3.5 to 4.0N in the 4oC and 4oC and 20oC mixed treatment until 30 days of storage, and was higher than that of the commercial variety. Therefore, it was suggested to be excellent in storability.

As the importance of public health increases with the spread of infectious diseases, functionality has become a factor affecting consumers’ purchase of mushrooms. Therefore, the bioactive components of button mushrooms (Agaricus bisporus), which are generally known to promote button mushroom consumption, were analyzed. White and brown button mushrooms were compared and white beech mushroom (Hypsizygus marmoreus) were used as a control. White button mushrooms had higher sugar and inorganic potassium concentrations than brown button mushrooms, whereas sodium, magnesium, and vitamin C concentrations were not significantly different between the different button mushrooms. Moreover, there was approximately twice as much ergosterol in white button mushrooms than brown button mushrooms. Brown button mushrooms had higher concentrations of β-glucan and oxalic acid than white button mushrooms, but there was no significant difference in total organic acid content between the two mushroom types. High concentrations of the essential amino acids, ergothioneine, isoleucine, and leucine and the non-essential amino acids, glycine and alanine, were observed. Concentrations of the vitamin B group and total polyphenols were also high.

본 연구는 인천광역시 강화군에 위치한 퇴모·혈구산의 관속식물상과 강화도 및 주변 지역의 외래식물 등을 파악하여, 조사된 식물들의 체계적인 관리와 생물다양성 보전을 위한 기초자료로 활용하기 위하여 조사를 실시하였다. 조사는 2019년 3월부터 2020년 10월까지 총 13회에 걸쳐 실시되었다. 퇴모·혈구산 일대의 관속식물상은 총 101과 321속 517종 13아종 38변종 5품종 573분류군으로 파악되었다. 이는 우리나라 관속식물 총 4,641분류군의 약 12.35%로 나타났다. 퇴모·혈구산에서 확인된 한반도 북방계식물은 청나래고사리 등 68분류군, 한반도 특산식물은 늦둥굴레 등 10분류군, IUCN 지정 희귀식물은 께묵 등 총 9분류군이 확인되었다. 식물구계학적 특정식물은 총 69분류군으로 파악 되었다. Ⅳ등급은 뚝사초 등 3분류군, Ⅲ등급은 종덩굴 등 14분류군이 확인되었다. 연구 대상지에서 출현한 귀화식물은 개망초, 돼지풀, 망초 등 63분류군으로 확인되었다. 개망초, 돼지풀, 망초 3종은 강화도뿐만 아니라 주변 지역인 석모도, 교동도 김포 등에도 전방위적으로 확산된 것으로 파악되었다. 그리고 가시상추, 가시박, 애기수영 등 생태계교란식물이 퇴모·혈구산에 처음으로 보고되어 퇴모·혈구산 내 특기할만한 식물 등 자생식물에 대해 중장기적인 보전 대책을 수립하 고 정기적으로 물리적인 제거 등의 관리방안 대책을 조기에 마련하여야 할 것이다

PURPOSES : High concentrations of particulate matter (PM) are emitted or generated from vehicle emissions in urban roads with dense transient populations. To reduce the effect of PM emission on bus stop users at roadsides, a plan to reduce PM emitted from the roadside must be devised. In this study, an atmospheric environment at a roadside is simulated in a large-scale environment chamber, and a test for reducing PM around the bus stop is conducted by installing a bus stop adapted to a PM reduction system. METHODS : Exhaust gas is injected into the experimental and reference chambers using diesel and gasoline vehicles for roadside airquality simulations. The two vehicles are operated in an idle state without an acceleration operation to emit exhaust gas uniformly, and the initial conditions are achieved by injecting car emissions for approximately 40 min. The initial condition is set to 1 ppm of NOx concentration in the environment chamber. Between the two environment chambers, a bus stop adapted to the PM reduction system is installed in the experimental chamber to conduct a PM reduction experiment pertaining to the air quality around the roadside. The experimental progress is set as the start time of the experiment based on the time at which the initial conditions are achieved; simultaneously, the PM reduction system in the experimental chamber is operated. After the simulation is commenced, the PM concentration, which changes over time, is measured using a high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS) without additional injection of car emissions or pollutants. The HR-ToF-AMS measures the chemical composition of non-refractory PM1.0 (NR-PM1.0) in real time. RESULTS : The NR-PM1.0 compound (organic aerosol (OA), NO3 -, SO4 2-) increases by 160% compared with the simulated initial concentration up to T90min in both environmental chambers; this is speculated to be due to secondary formation. The reference chamber indicates a slight decrease or a steady-state after T90min, whereas the experimental chamber indicates a gradually decrease as the experiment progresses. The bus stop adapted to the PM reduction system reduces the amount of black carbon in the experimental chamber by 37% at 200 min. This implies that the PM emitted from the roadside is filtered via the PM reduction system installed at the bus stop, and cleaner air quality can be provided to passengers. CONCLUSIONS : The PM reduction system evaluated in this study can be detached from and attached to the outdoor billboard of a bus stop. Since it adopts air filtration technology that uses a high-efficiency particulate air filter, it can be maintained and managed easily. In addition, it can provide an atmospheric environment with reduced PM emission to passengers as well as provide a better air-quality condition to passengers waiting for public transportation near roadsides.