This study aimed to examine the effect of a mild elevation in serum cholesterol level in a porcine coronary overstretch restenosis model using a balloon angioplasty catheter or drug-eluting coronary stent. Pigs were divided into two groups and were fed a commercial normal diet (CND, n = 4) or a high-fat diet (HFD, n = 4) for 5 weeks. Coronary overstretch injury by balloon angioplasty or stent implantation was induced in the left anterior descending and left circumflex artery after 1 week of feeding. Histopathological analysis was performed at 4 weeks after coronary injury. During the experiment, the total cholesterol level in the HFD group increased by approximately 44.9% (from 65.9 ± 3.21 mg/dL at baseline to 95.5 ± 9.94 mg/dL at 5 weeks). The lumen area in the CND group was reduced in comparison with that in the HFD group after balloon angioplasty. After stent implantation, the injury score showed no significant difference. There were significant differences in the neointimal area (2.7 ± 0.33 mm2 in the CND group vs. 3.3 ± 0.34 mm2 in the HFD group, p<0.05), lumen area (2.6 ± 0.54 mm2 in the CND group vs. 2.0 ± 0.33 mm2 in the HFD group, p<0.05), and percent area stenosis (52.0 ± 7.96% in the CND group vs. 62.4 ± 5.15% in the HFD group, p<0.05). Body weight change was not different between the two groups. Increased serum cholesterol level activated vascular smooth muscle cell proliferation in the porcine coronary overstretch model.

Acute myocardial infarction (AMI) is considered the major cause of mortality in the world. Tremendous animal studies are performed to develop novel therapeutics, and this study aimed to induce porcine myocardial infarction model by using polyethylene terephthalate (PET). Coronary guidewire was placed in left anterior descending artery (LAD). The balloon angioplasty catheter was inserted at the back of the PET. The balloon catheter was carefully pushed forward, until the balloon marker was located in mid-LAD. Coronary angiography was performed pre- and post-occlusion at 28 days by C-arm. Histologic analysis of heart tissue was performed 28 days after inducing AMI. Thirty three pigs were anesthetized and underwent percutaneous coronary catheterization. All pigs were successfully embolized in mid-LAD by PET. Fifteen pigs died due to ventricular fibrillation during post-anesthetic recovery time, and overall experiment mortality was 45.5%. In 2,3,5- triphenyl tetrazolium chloride staining, gross finding of the ischemic heart lesion showed firm and white area of infarction associated with the apex and left ventricular posterior wall. Infarct on H&E-stained sections demonstrated a region without myocytes and rich with cardiomyocyte with atypical nuclei. Successful induction of AMI by using PET may provide the pathophysiological information of ischemic heart disease and improvement of therapy development for AMI.

루비깍지벌레 Ceroplastes rubens는 감귤에 피해를 주는 주요 깍지벌레 중 하나로 연 1회 발생하며 수액을 흡즙하는 직접적인 피해와 분비된 감로로 인한 그을음병 유발의 간접적인 피해가 발생하고 있다. 제주지역에서 루비깍지벌레 천적으로는 루비붉은깡충좀벌 Anicetus beneficus와 애홍점박이무당벌레 Chilocorus kuwanae 2종이 확인되었다. 1975년 일본에서 도입 방사한 천적인 루비붉은깡충좀벌이 현재까지 지속적으로 발생하고 있었으며, 루비깍지벌레 발생 억제에 영향을 미치고 있었다. 2018년 4월부터 12월까지 도내 유기재배 감귤원 (2개소)에서 황색끈끈이트랩을 이용 루비붉은깡충좀벌 성충의 시기별 발생양상을 조사한 결과 월동세대 성충은 5월 중순, 제1세대와 제2세대 성충은 각각 7월 중순과 8월 하순에 발생 정점을 나타내었다. 조사 포장에서 루비깍지벌레의 기생률은 9월 중순에 9.6%와 19.6%이었다.

Soybean peptide (SP) exhibited low intestinal absorption at oral administration due to its fragile structure under gastric digestion. Therefore, we have attempted to encapsulate peptide by cross-linkage interaction between positive charged chitosan (CS) or chitosan oligosaccharide (CSO) and negative charged peptide. The CS (or CSO) with SP nanoparticles were prepared by using ultrasonification technique. The objective of this study was to find the optimal processing method by changing concentration, pH, and homogenizing conditions. We measured physicochemical properties such as particle size, zeta-potential, encapsulation efficiency (EE%), release rate (RR) and antioxidant ability of samples. The results showed that the optimal processing method was using 0.5% (w/v) CSO (diluted by pH 3 Acetic acid buffer) mixed with 0.5% (w/v) SP (diluted by pH 6 buffer) by 9:1 ratio. Afterwards, using high-speed mixer at 12,000 rpm for 3 min, and then passed 2 times through an ultrasonicator (50% power, 3 min). In this way for processing, the particle sizes of CSO/SP nanoparticles were approximately 300 nm, zeta-potential were approximately 45 mV. In addition, the EE% and RR of CS/SP nanoparticles was higher than the CSO/SP nanoparticles. The increase in antioxidant ability of SP was attributed to the affected by CS/CSO microcapsules. In conclusion, this research can befoundation for the manufacturing process of CS/SP nanoparticles, and it was expected that the future application of this nanoparticle in food matrix.

Cryoprotectant is a substance used to protect biological tissue from freezing injury. However, there was few research paper on application of cryoprotectant in food stuff although its benefits was approved from the biological cell tissues. The objective of this study was to investigate the effect of the sugar addition as a cryoprotectant on the properties of frozen soybean sprouts. Before freezing process, the samples were blanched at 100°C for 1 min to observe the influence of blanching treatment. The blanched or non-blanched soybean sprouts was immersed in sugar solution as cryoprotectant, and continuously, the samples were frozen at -18°C for 24 h. Their physicochemical properties such as drip loss, hardness, color and cellular tissue were analyzed after thawing in running water. In our study, the drip loss of blanched sample without sugar was 43%, and comparatively, blanched one with sugar was 20% which was the lowest value among all samples. There was no significant difference of hardness between sample with sugar and without sugar. From our results, it was supposed that sugar can protect the soybean sprouts during freezing process regardless blanching process.

Excessive salt intake in body induces health risks resulting high blood pressure or heart diseases. Therefore, the low salt concentration and sale tasted food is needed by means of the modification of manufacturing process. The purpose of this study was to study the effect of inhomogeneous salt localization in bread to enhance the saltiness encapsulated salt. The 0, 0.5, 1, 1.5, and 2.0% of liposome encapsulated salt (LS) was added into the baking of white pan bread. The final salt concentration was adjusted at 2% by addition of salt. After baking the bread, the moisture content, loaf volume, fermentation rate, color, texture analysis, salt release rate and sensory test were measured. From this study, moisture content has no significant difference between control and treatments (p>0.05), except for 2.0% LS. Lightness of all treatments was higher than control (p<0.05), whereas, there were no significant difference in hardness (p>0.05). From the sensory test, the bread added 2.0% LS was showed the highest value from the salty taste. Moreover, it is related to the highest release rate of salt was represented at 2.0% LS. In conclusion, the salty intensity of bread can be enhanced by the salt localization using encapsulation of salt.

Recently, semi-dried sweet potato is popular as a natural snake for children’s dessert. The drying condition was optimized to obtain high quality of sweet potato by oven drying process. The mashed yellow and chestnut sweet potato was dried using the oven drier at different temperature (50, 60, 70, and 80°C) then evaluated for the moisture content, appearance observation, texture properties, and sugar contents and sensory test in every 2, 6 and 12 hours. During the dehydration and drying process, the ending point of moisture content divided in three zone from 0-2 hour, 4-6 hour and 8-12 hour. The moisture content was dramatically decreased from 0 hour - 8 hour, but after 8 hour there is no significant decrease. Yellow sweet potato dried at 80°C for 6 hours was investigated as good product base on the sensory test, hardness value, and color appearances as compared to chestnut potato.

아시아틱 나리의 인편삽으로부터 형성된 자구와 소구의 효율적인 비대를 위한 식재깊이, 배양토 및 야간온도를 구명하기 위하여 한국농수산대학에서 육성한 분화용 F1 품종 ‘Deep Red Liasong’과 ‘Yellow Liasong’을 이용하 였다. 자구(직경 8mm)를 3 ~ 4cm의 깊이로 식재하였을 때, 자구의 지름은 19.2 ~ 19.6mm, 높이는 21.5 ~ 21.9mm, 생체중은 33 ~ 3.4g으로 자구가 양호하게 비대하였다. 소구 (직경 15mm)의 6cm 깊이 식재는 구의 지름이 28.5mm, 높 이는 27.8mm이었고 무게가 9.2g으로 가장 양호하게 비 대되었다. 자구의 식재 배양토는 인공배양토 80%+부숙왕 겨20%에서 구의 지름이 21.4mm, 높이는 21.1mm였고 생 체중이7.5g으로 자구가 가장 크게 비대하였다. 소구의 비대도 인공배양토 100%와 인공배양토 80% +부숙왕겨 20%에서 양호하였다. 야간온도에 따른 자구의 비대는 10oC와 12oC에서 지름이 17.2 ~ 17.5mm, 높이는 17.8 ~ 18.7mm, 생체중은 4.2 ~ 4.9g으로 가장 양호하였다. 따라 서 자구는 인공배양토와 부숙왕겨를 80 : 20으로 혼용하여 3 ~ 4cm의 깊이로 식재하여 동절기 동안 10 ~ 12oC로 유 지하고, 소구는 인공배양토에 부숙왕겨를 80 : 20으로 혼 용하여 6cm의 깊이로 식재하는 것이 구의 비대에 효과 적이다.

국내 육성 분화용 아시아틱나리 일대 잡종 ‘Deep Red Liasong’의 효율적인 자구형성을 위하여 구의 부위와 인 편삽 조건을 조사하였다. 구의 부위에 따른 자구 발생률 은 중인편은 87.6%, 내인편은 83.0%로 외인편의 40.3% 보다 양호하였으나 자구형성 개수는 외인편이 많았다. 인 편의 식재 깊이에 따른 인편당 자구의 수는 2.6개에서 2.8개로 차이가 없었으나, 3cm 깊이에서 잎의 발생이 없 었다. 인편의 배양온도는 항온 23oC와 변온인 주간 23oC /야간 13oC에서 인편당 자구의 수가 2.4 ~ 2.6개로 다른 온도처리보다 많았고, 자구 및 뿌리의 생장도 양호하였 다. 관수 주기를 2일에서 6일까지 달리하였을 경우 인편 당 자구의 수가 2.6~2.8개로 차이가 없었으나 8일은 2.2 개로 다소 낮았다. GA3, NAA 및 IBA를 50mg • L−1에서 200mg • L−1 처리 했을 때 농도가 높아질수록 자구의 형 성이 감소하였으나 뿌리의 개수와 길이는 증가하였다. 자 구의 식재 방향에 따른 인편당 자구의 수는 1.8개에서 2.0개로 차이를 보이지 않았으나 등을 위로(upper to back) 향하게 식재 했을 때 잎이 발생하지 않았다. 인편을 상하 1/2과 1/3로 절단하였을 때 인편의 기부에서는 자 구형성이 2.3 ~ 2.4개로 대조구의 2.5개와 같은 수준이었으 나 자구의 크기 및 생장은 대조구보다 작게 나타났다. 상 단에서는 전혀 자구가 형성되지 않았다. 한편, 인편을 좌 우 2등분과 3등분을 하였을 때 인편 내에서 자구의 수 는 차이가 없었으나, 3등분보다 2등분이 자구의 수 및 생 장이 양호하였다. 분화용 아시아틱나리의 효율적인 자구 생산을 위해서는 구근에서 중인편을 3cm의 깊이로 등을 위로 향하게 식재한 후 배양 온도는 항온 23oC나 주간 23oC/야간 13oC에서 건조하지 않도록 유지해야 한다.

국내에서 유통되고 있는 절화장미 품종의 향기를 분석하고 분류하여 방향성 장미 육종을 위한 유전자원의 정보로 활용하고 향기 나는 장미 꽃의 이용도를 높이고자 수행하였다. 총 78품종을 주요 향기 성분에 따라 4개 그룹(aromatic compound, fatty acid derivative, terpene 및complex fragrance)으로 분류하였다. 각각의 그룹은 100개 이상의 향기성분의 조성과 함량이 다르게 나타났다. Aromaticcompound계 그룹의 주요향기 성분은 dimethoxytoluene,phenylethyl alcohol, trimethoxybenzene, ρ-anisaldehyde 등이며 ‘For You’, ‘Vital’, ‘Cool Water’, ‘Golden Gate’ 및‘Orange Flash’ 등 44품종이 이에 속하였다. Fatty acidderivative계 그룹의 주요 향기 성분은 hexanol, hexenal,heneicosane, docosane, nonadecane 및 nonadecene이며‘Green Beauty’, ‘Amor’, ‘Aqua’ 및 ‘Dreaming’ 등 13품종이 이에 속하였다. Terpene계 그룹의 주요 향기성분은 germacrene-D, geraniol 및 nerol이며 ‘Blue Moon’,‘Charming Easies’, ‘Extasy’ 및 ‘Lovely Blue’ 등 12품종이 이에 속하였다. Complex fragrance계 그룹은 위의3가지 그룹의 성분이 혼재되어 있었으며 ‘Boy Friend’,‘Pacific Blue’ 및 ‘Red Calypso’ 등 12품종이 이에 속하였다. 국내에 유통되고 있는 대부분의 절화장미 품종은 주로 aromatic compound계 그룹에 속하고 있었다.

The study was to examine the variation and pattern of colony components of Vollenhovia emeryi. The total of 38 colonies was collected from 10 locations, including 35 colonies from 9 locations in South Korea and 3 colonies from 1 location in USA. To know colony components variation, we studied the approximate colony size, the approximate number of reproductives: queens, gyne and male, the approximate number of brood during 7 months. The observation period was from September 2012 to March 2013 over the breeding season from July to August. As a result, the study has found that colony components of the 38 colonies are not constant. This also indicates that gynes and males appear during non-breeding season at least in the laboratory condition. There are 4 types of gynes and males emergence: both(31.6%), only gynes(2.6%), only males(28.9%), absence(28.9%). The number of gynes and males of each colony also shows variance. It indicates that Vollenhovia emeryi may show colony components continuum. In this species, fertilized eggs become worker females and males; worker females are diploid but males are haplid because of maternal genomic elimination. Unfertilized eggs become queens which are diploid by clonal reproduction. Therefore, each individual of the sex can exceptionally transfer its own genes. This unique haplodiploid sex determination may create the conflict between queen and male and cause colony components continuum.

Malignant transformation of mature cystic teratoma (MCT) is rare. Sarcomatoid carcinoma is a neoplasm comprising malignant mesenchymal cells and a conventional carcinomatous area. Here, we report on a case of sarcomatoid carcinoma arising from an MCT in the left ovary of a 45-year-old female. A unilocular cyst consistent with MCT was observed; however, a nodule within the cyst was confirmed from the resected ovary. Microscopically, the nodule showed both squamous cell carcinoma and pleomorphic sarcomatous components admixing with each other. Lining epithelial cells at the periphery of the main tumor showed squamous metaplasia. When a sarcomatous component is observed in the ovary tumor, it is important to find a squamous cell component, either benign or malignant.

To identify subspecies and stocks of minke whale meats purchased from Korean markets during 2005-2007, we first obtained their complete sequences of mitochondrial DNA cytochrome b and control region sequences, and compared these sequences to the corresponding sequences of the common minke whale (Balaenoptera acutorostrata), obtained from GenBank. From analyses with partial cytochrome b sequences (383 bp) and non-coding, partial control region sequences (463 bp), Korean mink whale meats are identified as products from the North Pacific minke whale (B. a. scammoni). In addition, the sequences of the partial control region from these meats showed G at site no. 298 and G or A at site no. 463, and the meats appeared to originate from the J stock within this subspecies. Thus, because the J stock has been protected since 1986, implementation of strict regulation measures to reduce their accidental fisheries by catch seems urgent. In addition, B. a. scammoni is distinct from B. a. acutorostrata, with an average Jukes-Cantor distance of 2.21% in the complete control region sequence analysis (935 bp) and 1.31% in the complete cytochrome b gene sequence analysis; the current results support the current subspecies classification, although further sequencing analyses with nuclear genes are necessary.