본 연구는 불법적으로 수산물에 사용될 수 있는 염료 18종에 대한 안전관리 강화를 위해 정량 및 정성 분석이 가능한 LC-MS/MS를 적용하여 검증하기 위해 수행되었다 . 확립된 시험법은 CODEX CAC/GL-71 가이드라인에 따라 직선성, 정밀성 , 정량한계 및 회수율 등을 통해 유효성을 확인하였다 . 대상시료에 1% 아세트산을 함유한 아세토니트릴로 추출 후 C18 과 PSA로 정제하였다 . 본 실험에서 정량한계는 0.002 mg/kg 수준으로 정량한계를 포함한 농도에 따라 검량선을 작성하였고 모두 0.98 이상의 직선성을 확인하였다 . 또한 정확성은 63%-112% 이고, 정밀도는 15% 이하로 재현성이 우수하였다 . 국내 유통 중인 수산물 124 건을 수거하여 개발된 분석법의 적용성 검증과 안전성을 확인하고자 잔류실태조사를 실시 하였고 그 결과 7건이 미량으로 검출 되었고 부적합은 없었다 . 확립된 시험법은 수산물 안전관리에 활용할 수 있을 것으로 사료되는 바이다 .

The establishment of porcine embryonic stem cells (ESCs) from porcine somatic cell nuclear transfer (SCNT) blastocysts is influenced by in vitro culture day of porcine reconstructed embryo and feeder cell type. Therefore, the objective of the present study was to determine the optimal in vitro culture period for reconstructed porcine SCNT embryos and mouse embryonic fibroblast (MEF) feeder cell type for enhancing colony formation efficiency from the inner cell mass (ICM) of porcine SCNT blastocysts and their outgrowth. As the results, porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days showed significantly increased efficiency in the formation of colonies, compared to those for 7 days. Moreover, MEF feeder cells derived from outbred ICR mice showed numerically the highest efficiency of colony formation in blastocysts produced through in vitro culture of porcine SCNT embryos for 8 days and porcine ESCs with typical ESC morphology were maintained more successfully over Passage 2 on outbred ICR mice-derived MEF feeder cells than on MEF feeder cells derived from inbred C57BL/6 and hybrid B6CBAF1 mice. Overall, the harmonization of porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days and MEF feeder cells derived from outbred ICR mice will greatly contribute to the successful establishment of ESCs derived from porcine SCNT blastocysts.

As a preclinical study, many researchers have been attempted to convert the porcine PSCs into several differentiated cells with transplantation of the differentiated cells into the pigs. Here, we attempted to derive neuronal progenitor cells from pig embryonic germ cells (EGCs). As a result, neuronal progenitor cells could be derived directly from pig embryonic germ cells through the serum-free floating culture of EB-like aggregates (SFEB) method. Treating retinoic acid was more efficient for inducing neuronal lineages from EGCs rather than inhibiting SMAD signaling. The differentiated cells expressed neuronal markers such as PAX6, NESTIN, and SOX1 as determined by qRT-PCR and immunostaining. These data indicated that pig EGCs could provide valid models for human therapy. Finally, it is suggested that developing transgenic pig for disease models as well as differentiation methods will provide basic preclinical data for human regenerative medicine and lead to the success of stem cell therapy.

At q = 1.81 ± 0.20 × 10-5, KMT-2018-BLG-0029Lb has the lowest planet-host mass ratio q of any microlensing planet to date by more than a factor of two. Hence, it is the first planet that probes below the apparent "pile-up" at q = 5-10 ×10-5. The event was observed by Spitzer, yielding a microlens-parallax πE measurement. Combined with a measurement of the Einstein radius θE from finite-source effects during the caustic crossings, these measurements imply masses of the host Mhost = 1.14+0.10-0.12 M⊙ and planet Mplanet = 7.59+0.75-0.69 M⊕, system distance DL = 3.38+0.22-0.26 kpc and projected separation a⊥ = 4.27+0.21-0.23 AU. The blended light, which is substantially brighter than the microlensed source, is plausibly due to the lens and could be observed at high resolution immediately.

The present study examined changes in surface shape and pore size observed in carbon black particles isothermally oxidized in an air atmosphere according to their burn-off ratio. Carbon black materials were fed into a horizontal tubular furnace in an air atmosphere when the inside temperature reached 600 °C. Subsequently, while changing the isothermal oxidation time, carbon black samples with different burn-off ratios were obtained, i.e., 10.5, 20.0, 30.4, 41.0, 49.9, 59.8, 71.1, and 81.0%. The scanning electron microscope analysis revealed that the observed carbon black particles were in the form of aggregated primary particles, and that there was no change in the particle size of these primary particles as the burn-off process proceeded. The latter observation supported the observation that pores were formed in the carbon black samples during the burn-off process. Notably, the Brunauer–Emmett–Teller analysis exhibited hysteresis curves, indicating that the corresponding adsorption isotherms were of IV-type. It was also found that the area of the hysteresis curves increased as the burn-off process proceeded. The specific surface area of the raw carbon black sample was 58.00 m2/g, while that of the 81.0% sample was about 4.1 times the figure at 240.27 m2/g. The total pore volume VT was 0.17 cm3/g for the raw sample, and it was much higher for the 81.0% sample at 0.58 cm3/g. The transmission electron microscope analysis showed that the raw carbon black particles had a spherical shape with a smooth surface, but inner pores were not observed. In the 49.9% sample, pores with a size of about 5 nm were observed inside carbon black particles. Notably, the size of the pores observed in the 81.0% sample was about 20 nm and the large pores were created by the collapsing and merging of the smaller pores by oxidation.

본 연구는 아이스플랜트의 기능성 소금 대체제로서의 가능성을 조사하고자 천연 및 정제소금과의 NaCl, Dpinitol, 총 페놀, 총 플라보노이드 및 DPPH 라디칼 소거능에 대한 기초자료를 비교 조사하였다. 아이스플랜트 동 결건조분말의 NaCl 함량을 분석한 결과 19%이었고 Dpinitol의 함량은 777 mg/100 g으로 천일염 및 정제염에 비해 NaCl 함량은 유의적으로 낮으나 D-pinitol을 함유하고 있어 건강기능성에는 도움이 될 것으로 사료된다. 또한, 아이스플랜트 동결건조분말의 총 페놀 및 플라보노이드 함량은 각각 115 mg/100 g 및 985 mg/100 g을 함유 하고 있어 DPPH라디칼 소거능의 항산화효과를 갖는 것으로 나타났다. 따라서 아이스플랜트 분말의 bioactive compound을 함유한 대체 소금 천연소재로서 가능성을 확인한 기초 연구를 제공하였다.

농촌진흥청 국립원예특작과학원에서는 2017년 빅립 형태의 분홍색계 팔레놉시스 ‘Lovely Angel’을 개발하였다. 2009년 백색 팔레놉시스 P. ‘Timonthy’와 빅립을 가지는 분홍색 P. World Class ‘Big Foot’을 모본과 부본으로 교배하였다. 2013년 생육이 우수하고 화색, 화형, 꽃대수 등 개화 특성이 우수한 ‘09056-12’를 개체선발하여 기내 화경배양을 통해 증식하였다. 2014년부터 2017년에거쳐 1차, 2차 특성검정을 통해 품종의 안정성과 균일성을 확인하여 ‘Lovely Angel’을 육성하였다. 이 품종은 밝고 선명한 보라빛 분홍색(RHS, PVG81C)을 띄며, 줄 무늬를 가지고 있고 설판이 큰 빅립 형태가 특징적이다. 안아피는 형태의 꽃은 길이와 폭이 각각 6.1, 5.6cm이며, 1개의 꽃대에 20.6개의 소화가 착생하여 꽃 수가 비교적 많이 달리고, 꽃대 길이는 31.8cm 이다. ‘Lovely Angel’는 생육시 잎은 반하수로 자라며 길이는 18.7cm, 폭은 6.8cm이다. 신품종인 ‘Lovely Angel’은 소형 분화용으로 이용 가능할 것이다.

본 연구는 Veronica속 20종의 생장 및 개화 특성을 평가하기 위해 2년 동안 국립수목원 식물자원연구과 육종온실에서 수행하였다. Veronica속 식물은 다양한 생장 및 개화 특성을 가지고 있었다. 꼬리풀 20종을 식물 형태 및 초장에 따라 분류하였다. 포복형의 10cm 미만 초장은 V. armena와 V. repens, 직립형 30cm 미만 초장은 V. gentianoides ‘Little blues’ 등 4종, 직립형 30~60cm 사이 초장은 V. gentianoides ‘Blue Streak’ 등 7종, 직립형 60cm 이상 초장은 V. incana 등 7종이었다. 대부분의 엽색은 초록색이었고 V. incana와 V. incana ‘Silbersee’는 잎에 흰 털이 있었다. 꽃대 수는 5.3개부터 80.7개, 화수는 4.5개부터 67.3개였고 개화일은 3월 초순부터 6월 중순이었다. 화서의 형태는 총상화서, 수상화서, 취산화서로 분류하였다. 화색은 보라색 13종, 분홍색 2종, 흰색 5종이었다. 2017년 모든 개체가 개화한 종은 3종으로 V. longifolia ‘Blue Shades’, V. spicata ‘Blue Bouquet’, V. subsessilis ‘Blue Pyramid’였고, 일부 개체만 개화한 종은 7종으로 V. armena, V. gentianoides ‘Little Blues’, V. longifolia ‘Alba’, V. prostrata ‘Nestor’, V. spicata, V. spicata ‘Alba’, V. spicata ‘Sightseeing’ 이었다. 모든 개체가 개화하지 않은 종은 10종으로 V. gentianoides ‘Blue Streak’, V. incana, V. incana ‘Silbersee’, V. longifolia ‘Pink Shades’, V. orchidea ‘Blue Fingers’, V. repens, V. schmidtiana, V. spicata ‘Blue Carpet’, V. spicata ‘Pink Goblin’, V. teucrium ‘Royal Blue’였다. 반면, 2018년에는 모든 종의 개체가 개화하였다.

Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.

The CC chemokine receptor 5 (CCR5) is a G protein-coupled receptor that regulates chemotaxis and effector functions of immune cells. It also serves as the major co-receptor for the entry of human immunodeficiency virus (HIV). Recently, CCR5 inhibitors have been developed and used for the treatment or prevention of HIV infections. Additionally, it has been identified that CCR5 controls bone homeostasis by regulating osteoclastogenesis and the communication between osteoblasts and osteoclasts. However, the effects of CCR5 inhibition on bone tissue in elderly patients are unknown. This study aimed to examine the bone phenotype of aged CCR5 knockout (KO) mice. Femoral and tibial bones were isolated from 12-month and 18-month old wild-type (WT) and CCR5 KO mice, and microcomputed tomography and histology analyses were performed. Twelve-month-old CCR5 KO mice exhibited a decreased trabecular bone mass and cortical bone thickness in both femoral and tibial bones compared with agematched WT mice. Eighteen-month-old mice also showed a decreased trabecular bone mass in femurs compared with control WT mice, but not in tibial bones. Unlike in 12-month-old mice, the cortical margin of femurs and tibias in 18-month-old mice were rough, likely because they were aggravated by the deficiency of CCR5. Overall, our data suggest that the deficiency of CCR5 with aging can cause severe bone loss. When CCR5 inhibitors or CCR5 inactivating technologies are used in elderly patients, a preventive strategy for bone loss should be considered.

Alopecia has emerged as one of the biggest interests in modern society. Many studies have focused on the treatment of alopecia, such as transplantation of hair follicles or inhibition of the androgen pathway. Hair growth is achieved through proper proliferation of the components such as keratinocytes and dermal papilla cells (DPCs), movement, and interaction between the two cells. The present study examined the effect of the hedgehog (Hh) signaling pathway, which is an important and fundamental signal in the cell, on the morphology and the viability of human keratinocytes and DPCs. Upregulation of Hh signaling caused a morphological change and an increase in epithelium-mesenchymal transition-related gene expression but reduced the viability of keratinocytes, while the alteration of Hh signaling did not cause any change in DPCs. The results show the possibility that the regulation of Hh signaling can be applied for the treatment of alopecia.