본 연구에서는 azoxymethane (AOM)과 dextran sodium sulfate (DSS)로 유도된 대장 발암과정에 대한 셀레늄의 방어 효과를 조사하였다. 셀레늄 결핍(0.02 ppm Se), 정상(0.1 ppm Se), 과다(0.5 ppm Se)사료를 12주간 식이로 급여하여 혈액검사와 대장암 발생의 초기단계인 aberrant crypt foci (ACF)수를 측정했으며, 암 발생율을 조사하였다. ICP-AES 를 사용하여 간의 셀레늄 농도를 측정하였으며, 또한 셀레늄포함 항산화효소인 glutathione peroxidase (GPx) 활성을 알아보았다. 또한 TUNEL assay와 PCNA, β-catenin에 대한 면역조직 염색을 수행하였다. ACF 수 및 종양 발생률에 있어서, 셀레늄과다사료를 급여한 군이 정상셀레늄사료를 급여한 군보다 낮았으며, 셀레늄결핍사료를 급여한 군은 오히려 ACF 수 및 종양 발생률이 높았다. GPx 활성은 셀레늄의 섭취가 과다한 군에서 높게 나타났으며, 이 때, TUNEL 에서 apoptotic positive cell이 증가하는 것을 확인했다. 또 한 셀레늄의 섭취가 과다한 군에서 PCNA와 β-catenin의 발현이 감소됨을 볼 수 있었다. 본 마우스 모델실험에서 셀레늄은 여러 기전에 의해 대장암 발생을 억제할 수 있을 것으로 사료된다.

The present study investigated the effects of follicle stimulating hormone (FSH) and human chorionic gonadotrophin (hCG) on the nuclear maturation of canine oocytes. Oocytes were recovered from mongrel female ovaries in various reproductive states; follicular, luteal or anestrous stage. Oocytes were cultured in serum-free tissue culture medium (TCM)-199 supplemented with various concentrations of FSH (Exp. 1: 0, 0.5, 1.0 or 10 IU) or hCG (Exp. 2: 0, 0.5, 1.0 or 10 IU) or both (Exp. 3: 1 IU FSH + 1 IU hCG) for 72 hr to determine the effective concentration of these hormones, and to examine their combined effect. After maturation culture, oocytes were denuded in PBS containing 0.1% (w/v) hyaluronidase by gentle pipetting. The denuded oocytes were stained with 1.9 μM. Hoechst 33342 in glycerol and the nuclear state of oocytes was evaluated under UV light. More (p<0.05) oocytes matured to MII stage when follicular stage oocytes were supplemented with 1 IU FSH (6.2%) compared with the control, 0.1 or 10.0 IU FSH (0 to 1.2%). Significantly higher (p<0.05) maturation rate to MII stage was observed in follicular stage oocytes supplemented with 1.0 IU hCG (7.2%) compared with the control or other hCG supplemented groups (0 to 1.5%). However, the combination of FSH and hCG did not improve the nuclear maturation rate of canine oocyte (2.4 %) compared with FSH (6.2%) and hCG alone (7.2%). In conclusion, FSH or hCG alone significantly increased the maturation of canine oocytes to MII stage.

Carbon molecular sieve (CMS) membranes were prepared by pyrolysis of polyimides having carboxylic acid groups and applied to the hydrogen separation. The polymeric membranes having carboxylic acid groups showed different steric properties as compared with polymeric membranes having other side groups (-CH3 and -CF3) because of the hydrogen bond between the carboxylic acid groups. However, the microporous CMS membranes were significantly affected by the decomposable side groups evidenced from the wide angle X-rat diffraction, nitrogen adsorption isotherms, and single gas permeation measurement. Furthermore, the gas separation properties of the CMS membranes were essentially affected by the pyrolysis temperature. As a result, the CMS membranes Prepared by Pyrolysis of polyimide containing carboxylic acid froups at 700℃ showed the H2 permeability of 3,809 Baller [1×10-10 H cm(STP)cm/cm2.s.cmHg], H2/N2, selectivity of 46 and H2/CH4 selectivity of 130 while the CMS membranes derived from polyimide showed the H2 permeability of 3,272 Barrer, H2/N2 selectivity of 136 and H2/CH4 selectivity of 177.

Polyvinylpyrrolidone을 포함하는 폴리이미드 전구체의 열분해 공정을 통해 탄소분자체막을 제조하였으며 열분해성 고분자를 포함하는 전구체를 통해 제조된 막의 구조 및 기체 투과 특성에 대해 연구 하였다. 열분해성 고분자를 포함하는 전구체의 열적 특성을 조사한 결과 열적으로 안정한 폴리이미드의 경우 550℃에서 분해되는 것을 확인할 수 있었으며 열분해성 고분자의 경우 400℃에서 분해가 시작되는 것을 TCA를 통해 확인하였다 제조된 탄소분자체막의 기체 투과 특성을 조사한 결과 최종 열분해 온도가 증가됨에 따라 기체 투과도는 감소하였으며 열분해성 고분자를 포함한 전구체로부터 제조된 탄소분자체막의 경우 기체 투과가 향상됨을 알 수 있었다. 열분해성 고분자를 함유하는 전구체로부터 550℃에서 열분해를 통해 제조된 탄소분자체막의 경우 O2 투과도 808 Barrer [10-10cm3 (STP)cm/cm2scmHg]과 O2/N2선택도 7을 나타내었다.

Rats are one of the most widely used animals in biomedical sciences because their metabolism and physiology are comparable to humans. In recent years, gene-targeted models have been developed using various animal species utilizing engineered nucleases such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas). It has recently become possible to efficiently transfect CRISPR/Cas into embryos via electroporation. However, electroporation can damage fertilized eggs; therefore, it is important to determine the optimal embryo culture conditions. A standardized approach for routine and reproducible rat transgenesis will render rat models more accessible for research. We performed experiments to obtain rat embryos with efficient superovulation and synchronization, and to investigate the appropriate medium conditions for pronuclear stage embryos subjected to electroporation stimulation for the introduction of engineered nuclease.

고무화합물 형태로 구성된 조영제의 병에 Syringe Connector의 Spike를 연결 시 고무의 찢김 정도를 알아보고 찢김 및 분쇄로 인한 합성고무의 혼입 유무와 분쇄된 합성고무가 검출 시 분쇄물의 크기를 실험을 통해 알아보고자 하였다. 그 결과 찢김 정도의 경우 Syringe Connector의 끝과 최초 접촉하는 앞면이 약 3.14±0.04 ㎜로 뒷면 보다 많이 찢겼으며, 실험 대상인 10 병의 조영제에서 평균 7 개에서 15 개로 모두 분쇄물이 검출되었다. 검출된 분쇄물을 이용하여 크기를 측정한 결과 평균크기는 약 7.89±0.31 ㎛이었다. 향 후 다양한 실험 및 분석방법을 통한 추가실험과 더불어 흡인된 분쇄물 차단을 위한 미세 필터타입 자동주입장치의 개발이 필요하며, 분쇄물 유입 시 치명적 사고를 대비하여 관련기관의 관심 또한 필요할 것으로 사료된다.

Gummy jellies are popular desserts or snacks and widely consumed by various age groups around the world. Consumers' new needs and desire for a healthy food have promoted supplementation of the functional food ingredient to snack foods. As corn concentrate (CC) possesses diverse functional activities, it may be beneficial to increase its consumption via supplementation into snack foods such as jelly. There has been a scarcity of reports on antioxidant potential of CC and how much level is enhanced which occurs upon making CC incorporated jellies. This prompted us to undertake the present investigation with the objective to evaluate the physical, sensory, and antioxidant characteristics of jellies containing various levels of CC (3%, 6%, 9%, and 12%). The pH level (6.98 to 6.45), moisture content (89.34 to 80.06%, w.b.), and lightness (L*) (20.79 to 16.50) decreased significantly while total soluble solids (1.04 to 2.48 °Brix), hardness (3.47 to 5.57 N), redness (a*) (-0.45 to 4.95), and yellowness (b*) (4.76 to 7.70) increased significantly with increasing levels of added CC (p<0.05). In addition, 2,2-diphenyl-1-picrylhydrazyl (0.62 to 3.45%) and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (0.22 to 1.88%) radical scavenging activities significantly increased (p<0.05), and they were well-correlated. Consumer acceptance test indicated that addition of 6% CC had a favorable effect on consumer preferences for most attributes. Based on our study, jelly with 6% CC is recommended for developing CC-added jellies with improved overall qualities without sacrificing consumer acceptability while taking advantage of the functional properties of CC.

Background : ROS produced by oxidative stress damaged endothelial cells, and cause a variety of vascular complications. In diabetic hyperglycemia state, ROS increase. The polyol pathway occur in diabetic complications, the excess glucose is absorbed into the polyol pathway when aldose reductase increased, NADPH changes it to sorbitol. Glutathione (GSH) removes ROS. GSH level is reduced by glutathione reductase, using NADPH as an electron donor. Activation of the polyol pathway decrease NADPH, and GSH also reduced. As a result, ROS is increased. In diabetic hyperglycemia state, Glycolysis increases. Effects of increased glycolysis, protein kinase C (PKC) is increased. NAD(P)H oxidase, stimulated by PKC-dependent pathway, increases ROS in the cell. In this study, we measured the ROS scavenging activity of 5 natural products (Lycii fructus, Astragalus membranaceus, Cassia Tora, Polygonatum odoratum, Rubus Coreanus), to confirm the efficacy as diabetic antioxidants. Methods and Results : We extracted 5 natural product by distilled water and ethanol. DPPH radical scavenging activity was significantly higher in Lycii fructus, Rubus coreanus. ABTS radical scavenging activity was better Rubus coreanus, Lycii fructus, Cassia Tora. In addition to, Rubus coreanus, Cassia Tora, Lycii fructus was comparatively higher reducing power activity than other natural products. And total phenolic and flavonoid contents were much higher in Rubus coreanus compared with other extracts. Conclusion : These results suggest that Lycii fructus, Rubus coreanus can be applied as diabetic antioxidant that prevent vascular complications caused by ROS.

To overcome the risk of the ovarian hyperstimulation syndrome (OHSS) in patients have polycystic ovarian syndrome (PCOS) and to prepare emergency fertility preservation in patients undergoing anticancer treatment, several researchers have reported IVM of oocytes retrieved from ovaries exposed by only hCG priming. However, the maturation rate and the developmental potential of embryos from IVM oocytes are significantly lower than those of oocytes matured in vivo. Here, we investigated the optimal time point for immature oocyte collection at post hCG only injection for in vitro maturation, in vitro fertilization and blastocyst formation. Immature GV oocytes were collected from 25 days old B6D2F1 female mouse at 12 hr, 14 hr, 16 hr or 24 hr post hCG injection. Oocytes were collected from antral or late secondary follicle by puncturing with 26 G needle. Collected oocytes were cultured in G2 medium with 10% FBS, FSH, estradiol, and hCG for 16 hr in vitro and subjected in vitro fertilization and further embryonic development. To examine follicular maturation, we estimated the numbers of primordial, primary, secondary follicle and antral follicle on ovaries of each time point post hCG. To confirm the optimal time point post hCG injection for collecting immature oocytes, we recovered the oocytes from each time point. There is no difference in the number of oocytes per mice. Oocytes collected at 14 hr post hCG injection were shown higher maturation rate to MII stage and blastocyst formation compare to other three groups (p<0.01). However, there is no difference in the maturation rate on the other three groups. Also, apoptotic signal with TUNEL assay or anti-PARP staining was not change in ovaries from all experimental groups. Granulosa cell proliferation test with anti Ki-67 or anti AMH was not show any difference. According to these results, there are no significant differences in four different time points at 12 hr, 14 hr, 16 hr or 24 hr of collection of immature oocytes in hCG primed mouse. However, oocytes from 14 hr post hCG injection showed higher percentages of maturation rate, in vitro fertilization rate, blastocyst formation.

A new black seed coat soybean variety, “Socheong” was developed at the Yeongnam Agricultural Research Institute (YARI) in 2006. The goal to breed the black seed coat soybean is to develop the cultivar with high yield, lodging tolerance, resistant to disease such as soybean mosaic virus (SMV), and bacterial pustule and seed size. Socheong was selected from the cross between Milyang 78, which was late maturing, susceptible to lodging and SMV and with large seed size and green cotyledon, and Peking which was tolerant to lodging and with small seed size. The preliminary, advanced and regional yield trials for evaluation and selection of this variety were carried out from 2002 to 2006. It has determinate growth habit, white flower, brown pubescence, brown pod color, black seed coat, green cotyledon, elongated flattened seed shape, oval leaf shape and small seed size (15.7 grams per 100 seeds), and it was 3 days later in maturity than the check cultivar Cheongjakong. Socheong was higher, in the seed quality of sucrose and total sugar contents (6.8 and 8.2%) and isoflavone contents (1,754 ㎍/g) than the check cultivar. Futhermore, it has good characteristics for mechanical harvest, such as lodging tolerance, pod shattering and stem diameter. It also has been identified to have resistance to soybean mosaic virus symptom which was the troublesome soybean diseases. The average yield of Socheong was 2.21 ton per hectare in the regional yield trials carried out in four locations of Korea among seven locations from 2004 to 2006, which was 5 percent lower than the check cultivar Cheongjakong.

A new black seed coat soybean cultivar, “Heugmi” was developed at the Yeongnam Agricultural Research Institute (YARI) in 2006. The goal to breed the black seed coat soybean is to develop the cultivar with high yield, lodging tolerance, resistant to disease such as soybean mosaic virus (SMV), and bacterial pustule and seed size. Heugmi was selected from the cross between Milyang 78, which was late maturing, susceptible to lodging and SMV and with large seed size and green cotyledon, and Milyang 68, which was middle maturing, resistant to lodging and SMV and with middle seed size and yellow cotyledon. The preliminary, advanced and regional yield trials for evaluation and selection of this cultivar were carried out from 2001 to 2006. It has determinate growth habit, purple flower, brown pubescence, black pod color, black seed coat, green cotyledon, spherical seed shape, oval leaf shape and middle seed size (24.8 grams per 100 seeds), and it was 2 days earlier in maturity than the check cultivar Cheongjakong. Heugmi was better in the seed quality of crude protein contents, sucrose and total sugar contents than the check cultivar. It also has been identified to have resistance to SMV which was the troublesome soybean diseases. The average yield of Heugmi was 2.53 ton per hectare in the regional yield trials carried out in seven locations of Korea from 2004 to 2006, which was 8 percent higher than the check cultivar Cheongjakong.

This paper is a study of the relationships between trade and transshipment in Korea. Through the analysis of the data collected, a comprehensive model has been developed to analyze and predict relationships between trade and transshipment. By using analyses of port and trade evolution in Asia, the model identifies some important results. An application of the model to forecast developments in selected regions in China is also included in this paper. The paper provides a basis for shipping companies to decide on appropriate transshipment port strategies, and provides important theoretical references for Korean ports' development Supported by Shanghai leading Academic Discipline Project, project Number: T0602.