The two-spotted spider (Tetranychus urticae) is an important pest of many agricultural crops. Laboratory bioassayswere conducted to determine the toxicity of Pyflubumide and Cyenopyrafen against three populations of T. urticae collectedfrom apple orchards. The adults and eggs of three populations all showed susceptibility to Pyflubumide and Cyenopyrafen,as the 50% lethal concentration (LC50) values were all lower than each of the recommended concentrations. There wereconsiderable differences in susceptibility of the acaricides between eggs and adults in different populations. Pyflubumideshowed highest toxicity to adults of Yeongju population (LC50 = 1.40 mg/l) and eggs of Andong population (LC50 =0.54 mg/l). Cyenopyrafen showed lowest toxicity to adults of Andong population (LC50 = 20.29 mg/l) while displayedhighest toxicity to their eggs (LC50 = 2.64 mg/l). These results suggested that T. urticae had not developed resistancein these three regions, Pyflubumide and Cyenopyrafen can serve as alternative chemical candidate for integrated mite managementin apple orchards.

By definition, endosymbionts reside in the hosts' cells and are maternally inheritted to offspring as the mitochondriondoes. Recent progresses reveal that some endosymbionts have evolved their manipulabilities of hosts' reproduction. Inthis nature, once an endosymbiont shares its host with others, their association may last long unless their evolutionaryinterest or hosts' fitness is undermined.We tested whether the association existed among the endosymbionts; Wolbachia, Spiroplasma, Cardinium and Rickettsia.The results show that there are some multiple infection cases but there is no obvious pattern in their association.

Korea has been importing large quantities of plant materials from countries annually all over the world. Accordingly,various kinds of pests have been also detected during import inspections. In addition, these days the weather in Koreahas been getting warmer and a number of imported subtropical and tropical crops are being cultivated in greenhouses.In these regards, conducting a monitoring survey by using pheromone traps is required in order to prevent introductionand settlement of invasive species to Korea. we provide a list of insects collected from five types of pheromone trapscollected while monitoring exotic pests over the last seven years. Data from the monitoring surveys of pheromone trapsfrom 2010 to 2016 was extracted from database of the Plant Quarantine Integration System (PQIS) developed by theAnimal and Plant Quarantine Agency (APQA). As a result of our review on the PQIS database, 119 species were identified.The families forming the main groups are Tephritidae, Tortricidae, Camillidae and Cosmopterigidae. We believe that researchersand inspectors in charge of quarantine need to be well aware of the species collected from monitoring traps.

The attraction of the tomato whiteflies Trialeurodes vaporariorum, to four type of traps combined with a colored stickyboard and an light emitting lamp was investigated in 2016 in an tomato greenhouse. The colors of the sticky boardswere yellow and the peak wavelengths of the light emitting traps were 470nm(blue), 590nm(yellow) and 450~625nm(white).The type of traps was designed to directly illuminate the plants with an light emitting lamp and set along the lowerpart of the crops whose each sticky board was directed to the plants. This experiments showed that T. vaporariorumadults on average preferred (p>0.05) traps in blue lights(110±3.2adults/trap) compared to traps in yellow lights (71±1.4adults/trap)and white light traps(45±1.1adults/trap). The blue light trap(470nm) showed the most attractive to T. vaporariorum adults,followed by a similarly attraction to the yellow light trap(590nm), whereas the control (no light trap) was little attractiveto T. vaporariorum adults.

The present study was carried out to assess the perception of entomophagy in the Korean population. 329 respondentsof varying age groups were included in a semi-structured questionnaire survey. Results revealed that altogether 88% ofthe respondents knew ‘entomophagy’ fully or partially which is higher than few other scientific reports examined consumeracceptance in different countries, probably suggesting a rich tradition of using insects for food and medicinal purposesin Korea. Males were found more inclined to accept insect as food while females were neophobic. A similar trend wasobserved in case of using insects as a food ingredient. Acceptance of insect as food and or food ingredients was foundmore among older people in comparison to younger people which could be presumably because of familiarity with ancestralknowledge. Taste or flavor was found the most important determinant factor followed by nutrition and environmental issues.The outcomes of the study advocate ‘awareness’ of food safety, environmental and health issues among consumers canbe an instrument for wide acceptance of entomophagy.

West Nile Virus (WNV) is transmitted by infected mosquitoes. Vector mosquitoes usually acquire these pathogens fromfeeding on an infected host, and transmit the pathogens to a naive host during feeding events. To understand the virustransmission dynamics and to survey WNV throughout country, the present study has been conducted. We collected mosquitoesat urban parks in Seongnam, Wonju, Gunsan, Daegu, and Tongyeong using CDC light trap with Dry ice from April toSeptemper in 2017 (mosquito collecting is on going). Among collected mosquitoes, blood-fed mosquitoes were conductedblood meal identification assay and the other mosquitoes were subjected to virus detection using real-time PCR method.A total of 2,290 mosquitoes representing 6 genera and 15 species were collected. The most dominant species was Culexpipiens complex (42.1%) followed by Aedes albopictus (15.1%), Ae. vexans nipponii (14.6%), Ochlerotatus koreicus (9.8%),Cx. orientalis (6.5%), and Armigeres subalbatus (4.4%). The blood meal source were of mammal (93.3%), and birds (6.7%).So far, no WNV has been detected in any mosquitoes.

The members of the genus Flavivirus are noteworthy, as they cause infectious diseases in humans, such as Zika, denguefever, yellow fever, West Nile, and Japanese encephalitis. Due to the increased awareness of the public health risk posedby flavivirus-infected mosquitoes, mosquito collections were performed in six urban parks of South Korea, as the parksare designated for human recreation but also provide suitable habitats for mosquitoes. We examined the diversity andabundance of mosquito species and conducted molecular diagnostics for the detection of flavivirus infections. Monthlycollections were carried out in each park from March to August in 2017. A total of 4,851 mosquitoes (5 genera and13 species) were collected using BG-sentinel traps and then investigated for flavivirus infections. Pathogenic flavivirusinfections causing human diseases were not observed in the field-collected mosquitoes. However, insect-specific flavivirus(ISF) infections were detected in several mosquito pools. ISF has been previously known to enhance or suppress the replicationof medically important flaviviruses in co-infected mosquito cells. In this study, partial sequences of ISF were analyzed.However, further studies are needed in order to determine its genetic characterization and biological function in vivo.

The species of the genus Elasmostethus Fieber, 1860, belonging to the family Acanthosomatidae, occurring in the KoreanPeninsula are reviewed. Through this study, six species are recognized; the occurrence of four previously recorded species,including E. brevis Lindberg, 1934, E. humeralis Jakovlev, 1883, E. interstinctus (Linnaeus, 1758), and E. nubilus (Dallas,1851) are confirmed; two species, E. rotundus Yamamoto, 2003, and E. yunnanus Hsiao & Liu, 1977, are recorded forthe first time from the Korean Peninsula. The record of E. kansuensis Hsiao & Liu, 1977, is based on misidentificationof E. yunnanus. Therefore E. kansuensis is deleted from the Korean fauna. Photographs of habitus and genital segmentsof the two newly recorded species are included, and diagnoses and a key to the Korean species are provided.

Invasive pests have posed an ecological threat as climate change has been accelerated, suggesting early prediction ofinvasive pests is required to minimize damages by them. As one of predictive tools, CLIMEX has been effectively usedin a few regions, including US, Australia, and Europe. It allows us to predict a species distribution on a local area inresponse to climatic conditions: and thus, potential distribution of invasive species, risk assessment of agricultural pests,and suitability of biological control agents have been tested by CLIMEX. In this study, we introduced how to use CLIMEXfor predicting a species distribution differed by climate change in terms of its functions, required data, and examplesof its application.

This study was designed to optimize ethanol extraction process of unfertilized corn silk (UCS) to maximize phytochemical contents and bioactivities. The response surface methodology (RSM) with central composite design (CCD) was employed to obtain the optimal extraction conditions. The influence of ethanol concentration, extraction temperature and extraction time on total polyphenol contents, total flavonoid contents, maysin contents, 2,2-diphenyl-1- picrylhydrazyl(DPPH) radical scavenging activities and tyrosinase inhibition were analyzed. For all dependable variables, the most significant factor was ethanol concentration followed by extraction temperature and extraction time. The following optimum conditions were determined by simultaneous optimization of several responses with the Derringer’s desirability function using the numerical optimization function of the Design-Expert program: ethanol concentration 80.45%, extraction temperature 53.49°C, and extraction time 4.95 h. Under these conditions, the predicted values of total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity and tyrosinase inhibition were 2758.74 μg GAE/g dried sample, 1520.81 μg QUE/g dried sample, 810.26 mg/100g dried sample, 56.86% and 43.49%, respectively, and the overall desirability (D) was 0.74.

Drought is one of the detrimental factors that impair plant growth and productivity. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in maize leaves in response to drought stress. Two-week-old maize seedlings were exposed to drought (DT) by suspending water supply. DEGs were screened after 3 days of DT-treated samples using the ACP-based technique. Several DEGs encoding 16.9 protein, antimicrobial protein, hypothetical protein NCLIV_068840, thioredoxin M-type were identified in maize leaves under drought stress. These genes have putative functions in plant defense response, growth and development. These identified genes would be useful for predictive markers of plant defense, and growth responses under drought stress in plants.

Salt stress is one of the most limiting factors that reduce plant growth, development and yield. However, identification of salt-inducible genes is an initial step for understanding the adaptive response of plants to salt stress. In this study, we used an annealing control primer (ACP) based GeneFishing technique to identify differentially expressed genes (DEGs) in Italian ryegrass seedlings under salt stress. Ten-day-old seedlings were exposed to 100 mM NaCl for 6 h. Using 60 ACPs, a total 8 up-regulated genes were identified and sequenced. We identified several promising genes encoding alpha-glactosidase b, light harvesting chlorophyll a/b binding protein, metallothionein-like protein 3B-like, translation factor SUI, translation initiation factor eIF1, glyceraldehyde-3-phosphate dehydrogenase 2 and elongation factor 1-alpha. These genes were mostly involved in plant development, signaling, ROS detoxification and salt acclimation. However, this study provides new molecular information of several genes to understand the salt stress response. These genes would be useful for the enhancement of salt stress tolerance in plants.

Xenotransplantation is proposed as a solution to the problem of organ shortage. However, transplantation of xenogeneic organs induces an antigen-antibody reaction in α-1,3-gal structure that are not present in humans and primates, and thus complement is also activated and organs die within minutes or hours. In this study, we used FasL gene, which is involved in the immune response of NK cell, and US11, which suppresses MHC Class I cell membrane surface expression, to inhibit cell mediated rejection in the interspecific immunity rejection, and also hDAF(CD55) was introduced to confirm the response to C3 complement. These genes were tranfeced into Korean native pig fetal fibroblasts using pCAGGS vector. And cytotoxicity of NK cell and human complement was confirmed in each cell line. The US11 inhibited the cytotoxicity of NK cell and, in addition, the simultaneous expression of US11 and Fas ligand showed excellent suppress to T-lymphocyte cytotoxicity, hDAF showed weak resistance to cytotoxicity of natural killer cell but not in CD8+ CTLs. Cytotoxicity study with human complement showed that hDAF was effective for reducing complement reaction. In this studies have demonstrated that each gene is effective in reducing immune rejection.

This study focuses on exploring how to close attitude-behavior gap of consumers with regards to both recycled and upcycled fashion products. Specifically, this study is to explain the purchase gap between purchase intention and purchase experience of these sustainable products. Although many consumers had positive attitudes toward sustainable products, they often ended up not purchasing them. Factors including perceived values, risks, environmental concern, perceived consumer effectiveness, subjective norms, and demographic variables were examined to understand the discrepancy. The results indicate that antecedents of purchase intention were different from those of purchase experience and that influencing factors for recycled products were different from those for upcycled products. This study provides insights into understanding consumers and developing effective strategies to encourage desirable behaviors.

With the increasing popularity of social media, opinion leaders who have lots of followers have recently created strong advertising effects and generated greater sales. The emergence of online stars, Wang Hong in China, comes at a time when self-media gains popularity and e-commerce requires innovative ways to endorse brands in fashion industry. This study examines how these influencers impact consumers’ attitude toward the products they use and recommend. Specifically, this study investigates whether and how source attributes, social media content characteristics and judgment of product fit affect attitude of Chinese fashion consumers. As the perceived fit between the endorser and the product positively affect source credibility (Kamins, 1990) as well as consumer attitude toward the advertised product (Till & Busler, 2000), the present study hypothesized that the fit between the online star and the product will increase the perceived endorser credibility and product attitude of consumers. The effect of the fit between the product and the self-media content on the attitude toward the content or the product was also examined. Implications for managing marketing strategies as well as future research directions are subsequently discussed.

The aim of this study was to observe the effects of kinesiotaping and joint mobilization on the metatarsophalangeal joint angle and pain in hallux valgus patients Twenty-one female hallux valgus patients in their 20s were divided into two groups, a Kinesiotaping group (KT, n=10) and another group with the addition of joint mobilization (KTJM, n=11). After undergoing 6 weeks of intervention, the change in the metatarsophalangeal joint and pain were measured. Metatarsophalangeal joint angle was significantly increased both the KT and the KTMJ group after intervention. In the change of pain, both the KT and KTJM groups on an individual basis also experienced a significant decrease in pain, though comparison between the two groups failed to represent a significant difference. These findings suggest that Kinesiotatping and joint mobilization increased the joint angle and reduced pain.

Anthriscus sylvestris (L.) Hoffm. is a perennial herb found widely distributed in various regions of Korea, Europe, and New Zealand. The root of A. sylvestris have been extensively used in the treatment for antitussive, antipyretic, cough remedy in Oriental medicine, but the physiologically active function of the leaf of A. sylvestris is as yet unknown. In this study, we investigated the anti-cancer activity and the mechanism of cell death of water extracts of leaf of Anthriscus sylvestris (WELAS), on human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that WELAS treatment inhibited cell viability in a concentration- and time-dependent manner. In addition, the treatment of WELAS markedly induced apoptosis in FaDu cells, as determined by the viability assay, DAPI stain and FACS analysis. WELAS also increased the proteolytic cleavage of procaspase-3, -9 and PARP (poly(ADP-ribose) polymerase). In addition, exposure to WELAS decreased the expression of Bcl-2 (an anti-apoptotic factor), but increased the expression of Bax (a pro-apoptotic factor), suggesting that mitochondria-dependent apoptotic pathways are mediated in WELAS-induced apoptosis. Taken together, these results indicate that water extracts of leaf of A. sylvestris inhibits cell growth and induces apoptosis via the mitochondrial-dependent apoptotic pathway in FaDu human hypopharyngeal squamous carcinoma cells. Therefore, we propose that the water extracts of leaf of A. sylvestris is a novel chemotherapeutic drug, having growth inhibitory properties and induction of apoptosis in human oral cancer cells.