Radish, Raphanus sativus(2n = 18), belonging to the brassicaceae family, is herbaceous plant with 1-2 years life cycle. It is cultivated worldwide for producing leafy and root vegetables. Although an economically important crop, the genetics of yield and quality traits, disease resistance are not well-studies. The major purpose of this project is development of molecular breeding technology in radish. In this project, quantitative trait loci (QTL) for Fusarium wilt resistance of radish were analyzed. To identify QTL, genetic linkage map of radish was constructed using F2 mapping population derived from a cross between two inbred lines, “DB01” (resistant) and “DB05” (susceptible). A total 319 markers have been mapped into nine linkage groups, covering 639.3cM with an average distance of 2cM between loci. QTL mapping detected 2 loci conferring Fusarium wilt resistance. Two QTLs were located on LG3 and LG7, respectively. The QTL of LG3, flanked by EAGGMCT6 and WALK500 marker, exhibited a LOD value ranging from 2.3 to 8.7, and the R2 (Phenotypic variations) ranging from 28 to 48% in four tests. This QTL was named qYR1. The QTL of LG7, flanked by EACCMCAC-202 and DCJ14-390 marker, exhibited a LOD value ranging from 6.2 to 10.6, and the R2 ranging from 42 to 55% in four tests. This QTL was named qYR2. The results of the QTL analysis may be useful in marker-assisted selection (MAS) of Fusarium wilt resistant radish cultivars.

Anthocyanin has antioxidant and radical-scavenging effects which may protect cells from oxidative damage and reduce risk of cardiovascular diseases and cancer. A new peanut variety “Heuksaeng”(Arachis hypogaea ssp. hypogaea L.) with dark purple peanut skin was developed at the Department of Southern Area Crop Science, NICS, in Milyang in 2014. This variety was developed from the crossing line between cultivar “Iksan 31” with short stem and erect plant type and “Iksan35” with large grain and purple skin. “Heuksaeng” which is semi erect Virginia plant type has 32cm of main stem length and 25 branch number per plant. This also show more resistant to late leaf spot, web blotch and lodging, compared with check variety “Daekwang”. Each pod has two grains with ellipse shape of purple testa and its yield components is composed of 60 mature pods of per plant, 69g of 100-seed weight, 77% of pod shelling ratio in the regional yield trials(RYT). For 3 year regional yield trials the average kernel yield of “Heuksaeng” had 4.25 MT/ha similar to that of check variety. Its seed quality show 26.9% of crude protein and 46.0% of crude oil and 53.4% of oleate in fatty acid composition. Peanut skin of variety “Heuksang” consist of 2 kind of anthocyanin compounds such as 4.67mg/100g of delpinidine-3-glucoside (D3G) and 1.18mg/100g of cyanidine-3-glucoside(C3G). Peanut variety with high anthocyanin conent in skin will be useful to the recent preference of colorful food with healthful functional compounds.

Peanut is grown worldwide in the tropics and temperate zones primarily as an oilseed crop (38-54%) and protein source(25-30%). A new peanut variety “Daan”(Arachis hypogaea ssp. fastigiata L.) with the high yield potential was developed at the Department of Southern Area Crop Science, NICS, in Milyang in 2014. This was developed from the crossing line between cultivar “Sangpyeong” with short stem and high quality and “Dakwang” with large grain. “Daan” which is Shinpung plant type has 44cm of main stem length and 13 branch number per plant. Each pod has two grains with long ellipse shape of brown testa and yield components is composed of 34 mature pods of per plant, 127g of 100-seed weight, 75% of pod shelling ratio in the regional yield trials(RYT). Seed quality showed 47.8% of crude oil and 28.3% of protein content. This variety also showed more resistant to early leaf spot, late leaf spot, web blotch, stem rot and lodging compared with check variety “Daekwang”. In the regional yield trials “Daan” outyielded check variety by 16% with 5.00 MT/ha for kernel yield.

Shoot-fresh-weight (SFW) is one of the parameters, used to estimate the total plant biomass yield in soybean. Understanding the genetic and molecular basis of SFW could help increase the total biomass production. In this particular study, we identified QTLs associated with SFW in a Recombinant Inbred Line (RIL) population derived from interspecific cross of PI483463 and Hutcheson. A total of 551 (535 SNP and 16 SSR) markers, were found to be polymorphic between the parental lines and were used to screen the RILs to develop the genetic map. Linkage analysis and QTL mapping were performed using with the software QTL IciMapping version 4.0, with the minimum LOD score of 3.0 and estimating the likelihood of a QTL and its corresponding effects at every 1cM. QTLs with LOD value > threshold LOD, as determined by 1000 permutation tests at p > 0.05 were considered as significant QTLs. The analysis identified a total of 5 QTLs associated with shoot fresh weight over two environments, with the phenotypic variation (PV) ranging from 6.34 to 21.32%, and the additive effect from -0.54 to 0.33. Among these QTLs, qFW1314_19_1 had the largest LOD scores, with PV of 21.32%. Interestingly, three QTLs, qFW2013_19_1, qFW2014_19_1, and qFW1314_19_1 identified on chromosome 19(L), showed negative additive effects, indicating the contribution from the wild parent PI483463. The QTLs identified in this study can be the targets to identify the candidate genes for the SFW and can help in developing cultivars with increased biomass potential.

The purpose of development new variety ‘Miho’ (Hordeum vulgare L.) is a favorite with livestock feed and develop varieties resistant to disease and lodging. ‘Miho’ was carrying the growth habit of group Ⅲ, green and mid-wide leaf. Awn that are related to preference of livestock is a semi-smooth awn. This cultivar had 96cm of culm length, 650 of spikes per m2. Heading date of ‘Miho’ is April 27, and maturing dates on May 30, which were later than cultivar ‘Youngyang’. It also showed strong winter hardiness, and similar resistance to shattering and BaYMV compared with those of check one. The best thing among the traits of one is a new good quality with the plant green at the latter growing period. The average forage dry matter yield in the regional yield trial was about 13.1, 12.1 MT per ha in upland and paddy field, respectively, which were 9%, 2% higher than that of the check cultivar. It’s also showed 6.8% crude protein, 27.1% ADF (acid detergent fiber), and 67.5% TDN (Total Digestible Nutrients), including higher silage quality for whole crop barley. This cultivar would be suitable for the area whose daily minimum temperature was above -8℃ in January in Korean peninsula.

The aim of this study is to evaluate the possibility of improvement quality of a city inhabitancy and comfort creation in a daily life in an environment of population in developing cities by reconstruction an existing engineering - architectural building and a construction and creations of new intellectual architectural projects.

Reactive oxygen species (ROS) are produced in organisms as the natural products of oxidative metabolism by environmental stress and pathogen invasion. ROS, such as superoxide anion and hydrogen peroxide, can be toxic to cells and tissues to cause oxidative stress. Recent study revealed that olive flounder (Paralichthys olivaceus) superoxide dismutase (SOD) has been identified as a partial gene and strongly induced to benzoin[a]pyrene and it was deduced indicator of aquatic oxidative stress responses, but its transcriptional response against viral infection has not been investigated. In the present study, spatial and temporal expression profile was analyzed to investigate the function of Of-SOD in the anti-viral response. Of-SOD transcripts were ubiquitously detected in diverse tissues with variable levels using a real-time PCR. The expression of Of-SOD was significantly higher in the muscle, liver and brain, but extremely low in the stomach and spleen. Following VHSV challenge, the expression of Of-SOD increased within 3 hours and subsequently decreased to the original level at 2 days post-challenge in kidney. Although expression pattern and induction time are slight differences depending on the tissue, the transcript of Of-SOD was consistently increased in acute infection response, but expression is low in the chronic response. Collectively, Of-SOD expressions were inducible after VHSV infection and they were probably involved in the immune response against viral challenge. These results suggest that SODs may play important roles in the immune defense system of P. olivaceus and perhaps contribute to the protective effects against oxidative stress in this flounder.

Molecular markers are useful for selecting to include superior character genetic like as strong immune system and rapid growth in fish. The marker is also very important part of breeding technology in Olive flounder (Paralichthys olivaceus). Single nucleotide polymorphisms (SNPs) marker is already in use widely for genomic research and breeding. But this SNPs marker hardly has been validated for screening functional genes in Olive flounder. We study identify single nucleotide polymorphisms (SNPs) on Expressed sequence tag (EST) database, develop usable SNP marker and apply to wild sample and cultured of olive flounder. As a result, Out of total 4.327 ESTs, 693contigs and 514 SNP from total contigs were detected while these substitutions include 297 transitions and 217 transversions. 144 developed markers were applied in 16 samples (wild 8, culture 8), Out of total marker, only 32 markers had detected polymorphic in sample. Polymorphism of 32 markers was observed in the variety genes region involved in immunity and protein synthesis. And the 32 marker were identified 21 transitions, 11 transversions, and indel was not detected in polymorphic SNPs. The analysis on heterozygosity by sample showed 0.34 in wild sample and 0.29 in cultured sample. In conclusion, we was identified SNP and Polymorphism by designed new marker, it supports that development marker is suitable for SNP detection and diversity analysis in Olive flounder. The outcome of this study can be basic data for researches for immunity gene and characteristic with SNP.

As a preliminary investigation into the effect of environmental factors control for gonadal development, we examined the involvement of photoperiod and water temperature in ovarian development of Epinephelus. akaara. For the induction of sexual maturation, E. akaara reared in recirculating aquaculture system (RAS). During November 2013, the photoperiod and water temperature was adjusted to 12L:12D and 18℃, respectively. In the photo-thermal treatment group, every 3 weeks daylight was increased as follows a 13L:11D and 14L:10D, and control group was maintained under natural condition. After 9 weeks, water temperature was increased 23℃ in photo-thermal treatment group. The sampled fish every 3 weeks revealed increase in gonadosomatic index (GSI; 5.18±1.38), oocyte diameter and vitellogenic oocytes (423.9±36.1 ㎛) were observed in gonads 12 weeks under photo-thermal treatment group. However, ovarian development was maintained immature stage in control group. In this environmental factors manipulation trial, seventy one of the 95 females (578.4 ± 25.4 g in mean body weight, 31.0 ± 0.5 cm mean total length) treated with HCG injection (doses 500 IU/kg BW) were induced ovulation by artificial stripping. The total volume of ovulated eggs were 3,470 ml and the total volume of fertilized eggs was 3,295 ml. The fertilization rate and hatching rate were 95% and 98%, respectively. These results suggest that the photoperiod as well as water temperature are major environmental factors in triggering the gonadal development of E. akaara.

Legume and rhizobia symbiosis plays an important role in conversion of atmospheric dinitrogen to ammonia. On a global scale, this interaction represents a key entry point for reduced nitrogen into the biosphere, and as a consequence this symbiosis is important in both natural and agricultural systems. Symbiotic development of nodule organ is triggered by chito-oligosaccharide signals (Nod factors) from the bacterium which are perceived by the legume root. Understanding the molecular and cellular processes that underlie Nod factor perception is one focus of legume biology. Although forward genetics has proved to be an important tool to identify key players in Nod factor perception, we still know relatively little regarding the functional networks of genes and proteins that connect the earliest steps of Nod factor perception to immediate downstream outcomes. To elucidate genes and proteins that link Nod factor perception to cellular and physiological responses we are taking a discovery-based strategy based on whole transcriptome profiling using RNA-seq analysis in the roots of Medicago truncatula in response to Sinorhizobium meliloti. Functional characterization of a number of candidate genes is currently in progress to further examine their role in nodulation such as generating transgenic plants

Mungbean (Vigna radiata) is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. We constructed a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis and to enable a better understanding of the evolution of leguminous species. The draft genome sequence covers 80% of the estimated genome, of which 50.1% consists of repetitive sequences. In total, 22,427 high confidence protein-coding genes were predicted. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (Vigna reflexo-pilosa var. glabra) provided genomic evidence of a recent allopolyploid event. To further study speciation, we compared de novo RNA-seq assemblies of 22 accessions of 18 Vigna species and protein sets of Glycine max and Cajanus cajan. The species tree was constructed by a Bayesian Markov chain Monte Carlo method using highly confident orthologs shared by all 24 accessions. The present assembly of V. radiata var. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.

‘Muhan’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2013. It was derived from the cross between SB921011-3B-16-2/IT0188556 and ‘Suwon 365’. Among the cross made in 2002, a promising line, SB02T2097-B-B-B-18, showed good characteristics in potential forage yield in the yield trial tested at Iksan in 2009 to 2010 designated as Iksan 465. The line in the Regional Yield Trials(RYT) tested in eight locations around Korea for three years from 2011 to 2013, and was released as the name of ‘Muhan’. It has the growth habit of group Ⅳ, erect plant type, green leaf and awnless spike. Its average heading and maturing dates were on May 4, and June 4, respectively, with similar to check cultivar ‘Youngyang’. The cultivar had 102cm of culm length, 711 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Muhan’ was about 12.4 ton ha-1 in dry matter in paddy field. ‘Muhan’ also showed 6.6% of crude protein content, 28.6% of ADF (Acid Detergent Fiber), 50.1% of NDF (Neutral Detergent Fiber), and 66.3% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley

‘Jungmo2506’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2013. It was derived from the cross between ‘Sunwoo’ and ‘Milyang 34’. Among the cross made in 2002, a promising line, SB02T2020-B-BB- 64, showed good characteristics in potential forage yield in the yield trial tested at Iksan in 2009 to 2010 designated as Iksan 463. The line in the Regional Yield Trials(RYT) tested in eight locations around Korea for three years from 2011 to 2013, and was released as the name of ‘Jungmo2506’. It has the growth habit of group Ⅳ, erect plant type, green leaf and short awn spike. Its average heading and maturing dates were on May 3, and June 2, respectively, with similar to check cultivar ‘Youngyang’. The cultivar had 99cm of culm length, 692 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Jungmo2506’ was about 11.8 ton ha-1 in dry matter in paddy field. ‘Jungmo2506’ also showed 6.6% of crude protein content, 27.4% of ADF (Acid Detergent Fiber), 49.2% of NDF (Neutral Detergent Fiber), and 67.2% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley.

Soybeans have been the favored livestock forage for centuries. However, little studies have been succeed in estimating forage quality of soybean by near-infrared reflectance spectroscopy (NIRS). To establish NIR equations for soybean forage quality, 353 forage soybean samples, including an 181 recombinant inbred line population derived from PI 483463 (G. soja) ´ Hutcheson (G. max), 104 cultivated soybeans (G. max) and 68 wild soybeans (G. soja) were used to develop NIR for four quality parameters: crude protein (CP), crude fat (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF). Two NIR spectroscopy equations developed for CP and CF (2,5,5,1; multiple scatter correction [MSC]) and for NDF and ADF (1,4,4,1; MSC) were the best prediction equations for estimating these parameters. The coefficients of determination in external validation set (r2) were 0.934 for CF, 0.909 for CP, 0.767 for NDF, and 0.748 for ADF. The relative predictive determinant (RPD) ratios for MSC (2,5,5,1) calibration indicate that the CP (3.34) and CF (3.45) equations were acceptable for quantitative prediction of soybean forage quality, whereas the NDF (2.34) and ADF (1.97) equations were useful for screening purposes. The NIR calibration equations developed in this study will be useful in predicting the contents of forage qualities and in breeding soybean for forage

Olive flounder (Paralichthys olivaceus) is one of the commercial important flatfish species in Korea. The ocular signal transduction pathway is important in newly hatched flounders because it is closely involved in the initial feeding phase thus essential for survival during the juvenile period. However, the study of gene expression during ocular development is incomplete in olive flounder. Therefore we examined the expression analysis of specifically induced genes during the development of the visual system in newly hatched flounders. We searched ocular development-involved gene in the database of expressed sequence tags (ESTs) from olive flounder eye and this gene similar to arrestin with a partial sequence homology. Microscopic observation of retinal formation corresponded with the time of expression of the arrestin gene in the developmental stage. These results suggest that arrestin plays a vital role in the visual signal transduction pathway of the retina during ocular development. The expression of arrestin was strong in the ocular system during the entirety of the development stages. Our findings regarding arrestin have important implications with respect to its biological role and evolution of G-protein coupled receptor (GPCR) signaling in olive flounder. Further studies are required on the GPCR-mediated signaling pathway and to decipher the functional role of arrestin.

Cathepsins are members of the multigene family of lysosomal cysteine proteinases and have regulated function in several life processes. The potential role of cathepsin F cysteine gene was expected as protease in the yolk processing mechanism during early developmental stage, but expression analysis was unknown after fertilization. The alignment analysis showed that amino acid sequence of cathepsin F from olive flounder liver expressed sequence tag (EST) homologous to cathepsin F of other known cathepsin F sequences with 87－98% identity. In this study, we examined the gene expression analysis of cathepsin F in various tissues at variety age flounder. Tissue distribution of the cathepsin F mRNA has been shown to be ubiquitous and constitutive pattern regardless of age in each group, although derived from cDNA library using liver sample. The mRNA level of cathepsin F more increased as developmental proceed during embryogenesis and early developmental stage, especially increased in the blastula, hatching stage and 3 days post hatching (dph). As a result, it may suggest that the proteolysis of yolk proteins (YPs) has been implicated as a mechanism for nutrient supply during early larval stages in olive flounder.

Olive flounder (Paralichthys olivaceus) is a most important aquaculture species in Korea. Like most marine fishes, olive flounders are stomachless at first feeding and aquired gastric function during the metamorphosis, so food was mainly digested by pancreatic enzyme from first feeding to premetamorphosis. However, comprehensive analysis of pancreatic and gastric digestive enzyme of olive flounder at early developmental period is still unclear. In the expression study of pancreatic and gastric digestive enzyme by real-time PCR at early developmental stage, pancreatic enzyme such as chymotrypsinogen 2, preproelastase 2 and 4, pancreatic protein somatomedin-B domain (PPSB) mRNA expression were initiated at first feeding and strongly expressed in the pancreas developmental stage, while gastric digestive enzyme signal was not at all detected during same period. Although, trypsinogens were secreted from pancreas and have similar amino acid sequence, trypsinogen 3 expression induction was detected both pancreas and stomach developmental stage, while trypsinogen 2 expression was significantly increased only post-metamorphosis period. Pepsinogen mRNA expression was only detected at metamorphosis according to stomach differentiation. Lipid digestive enzyme, lipase and intestine fatty acid binding protein 1 (I-FABP 1), were already reached a certain level at beginning of hatching and more increased during early developmental stage and then gradually decreased before metamorphosis. These results suggested that feed ingestion of olive flounder was exclusive charged by pancreatic digestive enyme, lipid digestive enzyme and trypsinogen 3 from first feeding and then fully swiched by gastric digestive enzyme and trypsinogen 2 from metamorphosis period.

Legume and rhizobia symbiosis plays an important role in conversion of atmospheric dinitrogen to ammonia. On a global scale, thin interaction represent a key entry point for reduced nitrogen into the biosphere, and as a consequence this symbiosis in important in both natural and agricultural systems. Symbiotic development of nodule organ in triggered by chito-oligosaccharide signals(Nod factors) from the bacterium which are perceived by the legume root. Understanding the molecular and cellular processes that underlie Nod factor perception is one focus of legume biology. Although forward genetics has proved to be an important tool to elucidate key players in Nod factor perception, we still know relatively little regarding the functional networks of genes and proteins that connect the earliest steps of Nod factor perception to immediate downstream outcomes. To identify genes and proteins that link Nod factor perception to cellular and physiological responses we are taking a discovery-based strategy on large-scale transcriptome profiling using RNA sequencing in the roots of Medicago truncatula in response to Sinorhizobium meliloti. Functional characterization of a number of candidate genes is currently in progress to further examine their role in nodulation.

Green roof policies has helped to advance urban greening by creating green spaces on previously unused rooftops. However, there are no requirements covering matters such as greening methods, operation and maintenance obligations and many problems for sustainability and functioning of green roof and green space have been pointed out. This research is intended to clarify the contents and criteria of green roof policies currently implemented in Korea, Japan and other countries, compare them with the evaluation criteria for sustainable and public benefit rating systems and propose comprehensive indices for evaluating the multidimensional functions of green roof from the perspective of urban open space. As a result, it is found that obligation, grant, reimbursement, density bonus and tax credit are frequently used as green roof policies and 41 criteria including percentage of greening area, percentage of trees or shrubs, reduction of heat island effect, open space, landscape, and promotion of biodiversity are identified as criteria applicable to evaluating the multidimensional functions of green roof from the perspective of urban open space, respectively.