Sexual dimorphism is the most conspicuous difference between the sexes. This study examines possible sexual dimorphism and the relative growth patterns of morphometric characteristics in the marine medaka, Oryzias dancena for their potential to help differentiate between males and females of this species. The von Bertalanffy growth parameters estimated by a non-linear regression method were L∞=30.2 mm, K=3.22/year, and τ0=-0.05. All 18 characteristics measured showed a difference between males and females from 70 days after hatching. Each of these characteristics were significantly different between sexes (ANCOVA, P<0.05), and the ratio of standard length between sexes showed that males were larger than females for all five morphometric measurements. Fin length measurements were taken for 21 distances of anal fin and 7 distances of dorsal fin between landmarks. There were all differences for all dorsal fin rays between the males and the females and there is significant difference in 70 days after their hatch when the sexual dimorphism is presented. The significant difference (P<0.05) in fin ray for male and female was more greatly seen as they grow. Male marine medaka showed more rapid growth than females, with longer length, dorsal fins and anal fins. Differences in these characteristics will be useful during experiments when it is necessary to differentiate between sexes of marine medaka.

The sea-port in Northern Vietnam accounts for around one third of the total country’s cargo throughput and for many decades has been playing an important role in the logistics system of the country. 11 container terminals currently operate in Northern Vietnam, concentrating in Haiphong city and Quang Ninh province. Despite the increasing demand, the competition among these container terminals has become increasingly more critical. In recent years, massive investments from both government and operators have been made to improve the capability of the local sea-port’s logistics infrastructure. This critically needed comprehensive research evaluates the impact of the current logistics infrastructure condition on the competitiveness of terminals and quantitatively compares the competencies of these terminals. In order to meet such requirements, the paper first summarizes the indicators of the logistics infrastructures of the sea ports before developing and testing hypotheses to reveal the correlation between the given factors and the annual throughput of container terminals in the region. Factor analysis will then be applied to score the logistics infrastructure competency of each container terminal. A significant gap between logistics infrastructures among all container terminals was not observed and the competitiveness between container terminals is mostly driven by traditional activities including cargo handling and storage. According to the results, strategic thinking will be needed to contribute to related organizations for better decisions in investment, management, and operation.

Background : Recently, with the growth of traditional medicine industry, management of the quality of herbal medications as a raw material for functional food and medicine becomes more important. One of the most difficult problems of quality control of medicinal plants is management in storage insects. The problem of storage insects is a matter that must be resolved in order to safely obtain raw materials in herbal sector. But the basic prevention measures as well as grasp of the exact situation in the field are not yet. In this study, we investigated to see the problems and solutions through a review of storage pest management system of medicinal plants and the relevant provisions in herbal sector. Methods and Results : For this study, we examined the Status of domestic distribution, storage pest control techniques, news, domestic and import regulations. Storage pests that occur in herbal medications have dropped commercial value and it leads to consumer mistrust. A report said that consumers who had found at least once a pest and microbial contamination when they buy a herbal medications is more than 50% (2004, Ministry of Food and Drug Safety). In the past, those who want to solve these problems had tried through the sulfur fumigation. However, due to concerns about the risk to humans nowadays it was banned in many countries. According to the latest editions of Korean Pharmacopoeia (KP), it is allowed to use a suitable fumigant. but the domestic and imported medicinal plants during distribution can not be used it. Because currently registered fumigant (aluminium phosphide) is allowed only imported medicinal plants in the quarantine process. Chemical fumigation is inappropriate for use in foods and pharmaceuticals so the introduction of eco-friendly control methods is urgent. Eco-friendly methods of pest control that is currently being developed include a cold storage, packaging, high temperature and frozen method etc. Conclusion : These methods should be considered potential cost and Feasibility and it should meet the condition that stated “"Do not change the therapeutic effects of the herbal medications.” in the KP. Also supporting policy for expanding low temperature storage facilities in distribution area will raise possibility of success.

Background : Most of cases in korea, distributed herbal medications are storaged in room temperature and it causes many insect pests during storage. Despite the fact, there is little research about storage conditions. Temperature and humidity in storehouse are highly related with pests’ overwintering, generation length and the number of occurrence in summer. Consequently, in this research, major pests’ overwintering was studied by reviewing facility condition, temperature and humidity in major regional storehouses. The results of this would help to develop how to manage pest occurrence in distributed herbal medications. Methods and Results : For this study, storage facilities was investigated at different regions including Gyeongdong Market in Seoul, the pharmaceutical company in Daegu and farmhouse in Youngcheon by monitering temperature, humidity, ventilator, air conditioning and heating on ground and underground places from June 2014 to July 2016. including Upon investigation, most of building using for storage had simple ventilators. Whereas in case of farmhouse, it is vulnerable to maintaining quality. Because most farmhouse had temporary buildings which are difficult to control temperature and humidity. In addition, among the other surveyed warehouses, Basement in the Gyeongdong Market had most favorable condition for pest overwintering. The lowest temperature of it was seldom below zero maintaining level of about 1.5℃ and average temperature was about 10℃ in underground warehouse during winter. And the ground warehouse of Daegu had similar patterns in temperature and humidity with that of Seoul in that being over 0℃ even in the winter. In Youngcheon, the temperature of farmhouse was below zero degree and the daily range of temperature and humidity was wide. It caused difficulty of overwintering of insect pests but possible to inhabit. Conclusion : According to a study by Han-Mo, Koo(1997), it was impossible to overwinter for Lasioderma serricorne F. which is dominant medicinal plants’ pest in north of the Chungcheong region’s warehouse due to low temperature. But in case of investigated warehouses, it was possible to overwinter because of the high temperature in office building or basement storage area. In order to make prediction of pest occurrence and comprehensive measures, it is urgent to take more investigation by regional groups and types of storage.

Background : The purpose of this study is to investigate the effect of the ridge and mulching cultivation and adapted sowing(planting)time in stable medicinal herbs production of Lithospermum erythrorhizon Siebold et Zuc. And it accomplished one’s purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm’s income. Methods and Results : Method of cultivation type with the ridge and covering material were conducted under two condition compose to open cultivation (level row : 120㎝ ridging, black polyethylene mulching) and plastic greenhouse cultivation (high row : 90㎝ ridging, non-woven fabric mulching). Sowing with Daehong varieties in April 17, transplanting July 25 by growing pot seedling for 40 days in 2015. Planting density were spaced 30㎝ apart in inter-rows 10 ㎝ apart intra-row. Fertilizing level of N-P-K-Compost (fermented mixed oil cake fertilizer) applied at 6-4-6-300 ㎏․10 a−1, 60% of basal fertilizer in March 14, 40% of top dressing were four times application in April 14 ～July 15. Plot design randomized block 3 repetition. Conclusions : Compared to level ridging on black polyethylene mulching open cultivation for growing plastic greenhouse with the high ridging non woven mulching cultivation, seed yields havesting 1.4 times, the dried roots yields increased in 0.7 times. Also, Growth characteristics by sowing time in April 16. compared to planting time in July 25. Length of stem and main root of above-ground, subterranean part growth increment rising highest. Seed and dried roots yields with increased by 71%, 30%. respectively.

Background : Adenophora triphylla var. japonica Hara. is one of the important oriental medicinal herbs being used to cure lung/respiratory diseases. It belongs to the five Sam (ginseng) including Panax ginseng, Salvia miltiorrhiza, Sophora flavescens, and Scrophularia buergeriana. In 2015, Korea produced 72 tons of medicinal herbs in a 14-hectare area and imported 24 tons valued at US$95,000. This study was carried out to investigate the effect of several biopesticides to control leaf spot of A. triphylla var. japonica Hara. Methods and Results : One year plants at the Department of Herbal Crop Research, NIHHS, RDA were used. The treatments used biopesticides such as plant extracts (BP), microbial agent (DM), microbial agent (PJ), sulfur powder (HS), and controlled chemicals (ST), while non treatment (NT) was used as control. After the early part of disease outbreak, the plants were treated four times with 7 days interval. The incidence of leaf spot, efficacy, phytotoxicity and control value were investigated. Based on the efficacy test, incidence of leaf spot is lowest at 15.6% in plant extracts (BP). Other biopesticides showed higher incidence - 16.7% for microbial agent (DM), 17.8% for microbial agent (PJ), 18.9% for sulfur powder (HS) and 71.1% for non treatment (NT), compared with 11.1% in controlled chemicals (ST). The microbial agent (PJ), sulfur powder (HS), plant extracts (BP) and microbial agent (DM) were not damaged in the phytotoxicity test. In the test of application dose, time and methods, the control value was highest in plant extracts (BP) at 78.1%, followed by microbial agent (DM) at 76.6%, microbial agent (PJ) at 75.0%, and sulfur powder (HS) at 73.4% , compared with controlled chemicals (ST) at 84.4%. Conclusion : It is possible to use biopesticides at the proper time to prevent leaf spot in Adenophora triphylla var. japonica Hara.

Background : Adenophora triphylla var. japonica Hara. is used to cure respiratory/ lung diseases, which makes it an important oriental medicinal herb. It is one of the five Sam (ginseng), which includes Panax ginseng, Salvia miltiorrhiza, Sophora flavescens, and Scrophularia buergeriana. In 2015, Korea imported 24 tons of medicinal herbs worth US$95,000. This study was carried out to investigate the effect of several mulching materials on the growth and root yield of A. triphylla var. japonica using organic fertilizer. Methods and Result : Seeds of A. triphylla var. japonica were collected at the nursery of the Department of Herbal Crop Research, NIHHS, RDA in 2015. Mulching materials used are black plastic, black woven fabric, and biodegradable plastic. Hand weeding was done to serve as control. The soil was fertilized two months before planting. Seeds of A. triphylla var. japonica were sowed in tray in early March and were allowed to grow for two months. It was planted in early May with planting density of 30 ㎝ x 15 ㎝ and was covered with various mulching materials. Parameters investigated are survival rate, aerial part and root growth characteristics, and yield at harvest time. Results showed that biodegradable plastic is most favorable for plant growth with height of 17.2cm, leaf length of 13.4cm and leaf width of 5.6 ㎝. Dry plant weight was heaviest in biodegradable plastic treatment at 2.8 g, and lightest at 2.5 g when planted without mulch. Biodegradable plastic has the highest dry root weight of 19.3 g, followed by black vinyl (18.4 g), woven fabric (18.3 g) and without mulch (17.9 g). The yield obtained per 10 a was highest in biodegradable plastic treatment at 174 ㎏, while black vinyl and woven fabric treatments produced 169 ㎏ and 160 ㎏, respectively. Plants without mulch had the lowest yield of 157 ㎏. Conclusion : In the above results, biodegradable plastic and woven fabric are considered suitable mulching materials for the cultivation of A. triphylla var. japonica.

Background : Recently, there is a urgent demand for development of new varieties with enhanced resistance to various biotic/abiotic stresses. In order to develop ginseng varieties with such traits, systematic breeding program and comprehensive field studies are prerequisite. Methods and Results : ‘Cheonmyeong' seeds were collected in 2008 from the farmer field of Buyeo. Physiological investigation and propagation were conducted from 2009 to 2011. It was given the name 'Eumseong No. 8 through the observed yield trial from 2012 to 2013 and local adaptability was carried out from 2014 to 2015. All phenotypes including agronomic characteristics, seed yield, and physiological response to biotic/abiotic stresses were investigated according to the ginseng GAP and UPOV guidelines. Yellow-red leaf and pink berry at maturing stage were observed. The time of emergence, flowering and berry maturity of the ‘Cheonmyeong’ were faster than those of ‘Chunpoong’. Stem length of ‘Cheonmyeong’ was shorter than that of ‘Chunpoong’, whereas stem diameter was thicker than that of ‘Chunpoong’. Main root length was shorter but main root diameter is thicker than that of ‘Chunpoong’. Number of seeds of ‘Cheonmyeong’ was fewer than that of ‘Chunpoong’ but 1,000-seeds weight and stratification rate were higher than those of ‘Chunpoong’. The yield performance of this variety was 661 kg/10 a in local adaptability test for two years, which is 22% higher than that of ‘Chunpoong’. ‘Cheonmyeong’ showed strong resistance to phytophthora blight, mulberry mealybug and nematode and moderate resistance to alternaria blight. ‘Cheonmyeong’ did almost not occur yellow spot of aerial part and rusty skin of root, show moderate resistance at high temperatures. Conclusion : Our study demonstrated that ‘Cheonmyeong’ is an ideal variety with heavier root weight and enhanced stress resistance and contribute will enhance biotic/abiotic stress resistance and increase the farmers' income.

Background : Angelica gigas N., one of the herbs has been used most frequently with Glycyrrhiza uralensis. It is usually cultivated in semi-alpine of more than 400m in North Central province of South Korea. However its production is becoming more unstable due to the climate change especially abnormally high temperature. In particular, the environmental change may cause not only a change in production but also a change in active ingredient of Angelica gigas N. This study was performed to investigate these problems. Methods and Results : This study was carried out in temperature gradient tunnel(TGT) in Pyeongchang, Gangwon province. Temperature conditions were maintained the ambient+0~2℃ (T1), ambient+2~4℃(T2), ambient+4~5℃(T3) throughout the year. We planted and grown one year old seedlings of Angelica gigas N. from mid-April to early November. As a result, there was no difference in early growth of Angelica gigas N. among the temperature range but there was a difference between each treatment region from the survey in June. Average length of soot in T1, T2, T3 was the largest(36cm), medium(27cm), smallest(19cm) respectively. This trend was similar to a survey in September and November. Each of death rate was 0%(T1), 19%(T2), 71%(T3). There was no bolting according to the temperature. The yield of a plant of Angelica gigas N. was 476g(T1), 76g(T2), 26g(T3), respectively. Production of Angelica gigas N. is foreseen 84.7% and 94.5% reduction if the temperature rises to 3±1℃ and 4±1℃ more than it is at present based on temperature in Pyeongchang 2015. Total amount of Decursin was reduced at higher temperatures. Conclusion : The results of this study show that quality and production of Angelica gigas N. is expected to decline significantly if the temperature rise than the current. Therefore development of various techniques is required to response to climate change.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different covering material and weed eliminating on stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : method of weed elimination with covering material were conducted under four condition compose to Non covering(4～6 times by manual weed control), Barley straw cutting covered with 700㎏․10 a−1, Rice straw cutting covered with 500㎏․10 a−1, Rice husks covered with 1,000㎏․10 a−1. Fermented mixed oil cake compost applied at 380㎏․10 a−1, 60% of basal fertilizer in March 14, 40% of top dressing were four times application in April 14 ～July 15. Sowing dates were March 25, 2015. Plants were spaced 15 ㎝ apart in rows 15 ㎝ apart with open cultivation. Conclusions : Rice husks covering < Barley straw covering < Barley straw covering cultivation this order, aerial part as a result were plenty amount of growth. Weed the rates was 12 to 29% lower incidence. Green leaf yields is uncovering control plot (915 kg․10 a−1) compared to 2% from covering rice husks, barley straw and rice straw covering increased to 18%, 20%, respectively.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different organic fertilizer and applicated cultivation on stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : method of application with organic fertilizer were conducted under nine condition compose to control(non application), N-P2O5-K2O (20-12-10)㎏․10 a−1, Hyphal incubation compost(HIC) applied at 851㎏․10 a−1, Hangawi organic compost(HOC) applied at 547㎏․10 a−1, Fermented mixed oil cake compost(FMOC) applied at 380㎏․10 a−1, 60% and 40% of basal fertilizer in March 12, top dressing were four application in April 12, May 12, June 13 and July 12, respectively. Sowing dates were March 23, 2015. Plants were spaced 15 ㎝ apart in rows 15 ㎝ apart with open cultivation. Conclusions : chemical fertilizers < hyphal incubation compost < hangawi organic compost < fermented mixed oil cake compost application, top dressing than 60% in order of 40% additional fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Green leaf yields is untreated control plot (875 kg․10 a−1) compared to 12% from using chemical fertilizers, HIC application 18% higher, HOC application 20% higher, and FMOC application increased to 28% of the most.

Background : The roots of Curcuma longa Radix is used to make herbal medicine, which is registrated in the Korean Pharmacopoeia. This species is a perennial herb, and is distributed throughout tropical and/or sub tropical regions. High temperature and humidity with small difference between daytime and nighttime is the finest environment condition in this species. Recently, demand for C. longa has been expanded to cultivation area as central region from southern region. Therefore, this study aimed to investigate the effect of harvest time on the growth, yield characteristics and main composition contents in C. longa. Methods and Results : Curcuma longa rhizomes were harvested from farmland in Jindo, Jeollanam-do in end October, early November, middle November, end November, early December, middle December and end December 2015. Harvested rhizome were dried in hot air drier, and it was investigated as growth characteristics and yield. Contents of curcumin, demethoxycurcumin, bisdemethoxycurcumin was analyzed from dried powder of rhizome. The analysis column was used to Phenomenex, Kinetex 2.6um F5 100A 100*2.1mm. 10 mg of standard was taken, 80% MeOH was added to dissolve in 10 ㎖ flask. 0.5, 1, 2, 4 ㎖ was taken from dissolved solution and then was added to 10 ㎖ of 80% MeOH. It was filtered through 0.45 ㎛ filter and then 5 μℓ was taken to make standard solution. Analytical calibration curve was measured to diluted solution within sample concentration. 2 g of ground sample was taken, 40㎖ of 80% MeOH was added to extract by ultrasound sonication during 60 minutes and then it was filtered through 0.2 ㎛ filter to analyze component content. Conclusion : The root weight significantly increased from 376.1 g in end October to 695.9 g in end December as 1.8 double. Curcumin contents differed significantly among harvest times in end November and early December. Demethoxycurcumin increased from early November to end November, and Bisdemethoxycurcumin increased to the early November.

Background : Adenophora triphylla var. japonica Hara. is used to cure internal organ, which is called to important one of the five “Sam (ginseng)” oriental medicinal herbs included Panax ginseng, Salvia miltiorrhiza, Sophora flavescens, Scrophularia buergeriana. Korea imported 24 ton 129 thousand dollar for medicinal herbs and 23 ton 29 thousand dollar for food at 2013. This study was carried out to investigate the effect of several organic compost on the growth and root yield of A. triphylla var japonica with organic fertilizer. Methods and Results : Seeds were collected from Adenophora triphylla var. japonica Hara. at last year in nursery of Department of Herbal Crop Research, NIHHS, RDA. Four types organic fertilizer (germiculture (GG), granular mix oil cake (CJ), mixed organic (DJ), plant oil cake (TG)), chemical fertilizer (CF) and non-fertilizer (NF) were treated. It was fertilized on soil before 2 month of planting. Seeds A. triphylla var. japonica were sowed in tray at early March and then were grown during 2 month. It were planted in furrowed nursery at early with 30 × 10 cm planting distance and black vinyl covering condition. Soil chemical property, survival rate, aerial part and root growth characteristics and yield were investigated at harvest time. For main composition analysis, 10 mg of standard (b-sitosterol, lupenone) was taken, 80% MeOH was added to dissolve in 10 ㎖ flask. 0.5, 1, 2, 4 ㎖ was taken from dissolved solution and then was added to 10 ㎖ of 80% MeOH. It was filtered through 0.45 ㎛ filter and then 5 μℓ was taken to make standard solution. Analytical calibration curve was measured to diluted solution within sample concentration. 2 g of ground sample was taken, 40㎖ of 80% MeOH was added to extract by ultrasound sonication during 60 minutes and then it was filtered through 0.2 ㎛ filter to analyze component content. Conclusion : GG and DJ were the most suitable for organic cultivation of denophora triphylla var. japonica. Content of beta-sitosterol showed the highest value in DJ treatment and the lowest value in CF. Content of lupenone showed the highest value in DJ and the lowest value in CJ.

Vietnam’s sea-port industry has experienced a significant development in recent years. Especially in Northern Vietnam, both the demand and supply of handling services for containerized cargoes have increased at a considerable rates. Accompany with such movement, the competition among container terminals in the area becomes fiercer. In this paper, Hierarchical Cluster Analysis is employed to classify all 11 container terminals in Northern Vietnam by collecting data concerning terminal competitiveness. After the classification, each group will be discussed in order to reveal more details about their competitive characteristics. The paper consists of five sections. Section 1 is the general introduction. Section 2 provides a general literature review about competitiveness and factors to evaluate competitiveness. Section 3 explains variables and methodology applied to do the analysis. Section 4 presents the results with linkage to the current condition. Section 5 summarizes the analysis results. It is shown that container terminals in Northern Vietnam should not only pay attention to their service qualities but also have to find out an appropriate mechanism to avoid unhealthy competition. The paper is expected to contribute a background for further researches in container terminals’ competition in the region as well as hints for operators in planning and making decisions.

This study was conducted to develop a fast and efficient screening method to determine the quantity of fatty acid in peanut oil for high oleate breeding program. A total of 329 peanut samples were used in this study, 227 of which were considered in the calibration equation development and 102 were utilized for validation, using near infrared reflectance spectroscopy (NIRS). The NIRS equations for all the seven fatty acids had low standard error of calibration (SEC) values, while high R2 values of 0.983 and 0.991 were obtained for oleic and linoleic acids, respectively in the calibration equation. Furthermore, the predicted means of the two main fatty acids in the calibration equation were very similar to the means based on gas chromatography (GC) analysis, ranging from 36.7 to 77.1% for oleic acid and 7.1 to 42.7% for linoleic acid. Based on the standard error of prediction (SEP), bias values, and R 2 statistics, the NIRS fatty acid equations were accurately predicted the concentrations of oleic and linoleic acids of the validation sample set. These results suggest that NIRS equations of oleic and linoleic acid can be used as a rapid mass screening method for fatty acid content analysis in peanut breeding program.

The microbial composition in Nuruk, a Korean cereal fermentation starter, is a critical factor for the quality and organoleptic properties of traditional alcoholic beverages. This study was aimed at monitoring the compositional change and enzyme activity of culturable lactic acid bacteria (LAB) in two types of Nuruk fermented at different temperatures. All culturable LAB were isolated at various time points (0, 3, 6, 10, 20, and 30 days) and identified by 16S rRNA sequencing. In traditional Nuruk type A (TN-A), which was fermented at 36℃, the population of total culturable LAB during the fermentation period was between 104 and 105 log CFU/mL. On the other hand, the LAB population in traditional Nuruk type B (TN-B) fermented at 45℃ (primary fermentation for 10 days) and 35℃ (secondary fermentation for 20 days) was 102 log CFU/mL; however, these bacteria could not be detected after 6 days. Major LAB strains were identified in both Nuruk types: (1) from the MRS-culture of TN-A, Pediococcus pentosaceus at 3-30 days; (2) from MRS-culture of TN-B, P. pentosaceus at 3 days and Enterococcus hirae at 6 days. The protease activities of the dominant LAB isolated from the TN-A and TN-B cultures were within the ranges of 0.64~1.03 mg/mL and 0.74~0.81 mg/mL (tyrosine content), respectively, whereas the α-amylase activities were 0.75~0.98 mg/mL and 0.78~0.79 mg/mL (amylose content), respectively.

By using the Optical Wide-field Patrol (OWL) network developed by the Korea Astronomy and Space Science Institute (KASI) we generated the right ascension and declination angle data from optical observation of Low Earth Orbit (LEO) satellites. We performed an analysis to verify the optimum number of observations needed per arc for successful estimation of orbit. The currently functioning OWL observatories are located in Daejeon (South Korea), Songino (Mongolia), and Oukaïmeden (Morocco). The Daejeon Observatory is functioning as a test bed. In this study, the observed targets were Gravity Probe B, COSMOS 1455, COSMOS 1726, COSMOS 2428, SEASAT 1, ATV-5, and CryoSat-2 (all in LEO). These satellites were observed from the test bed and the Songino Observatory of the OWL network during 21 nights in 2014 and 2015. After we estimated the orbit from systematically selected sets of observation points (20, 50, 100, and 150) for each pass, we compared the difference between the orbit estimates for each case, and the Two Line Element set (TLE) from the Joint Space Operation Center (JSpOC). Then, we determined the average of the difference and selected the optimal observation points by comparing the average values.

The flower buds of Sophora japonica L (SF), as a well-known traditional Chinese medicinal herb, have been used to treat bleeding-related disorders such as hematochezia, hemorrhoidal bleeding, dysfunctional uterine bleeding, and diarrhea. However, no specific anti-cancer effect and its molecular mechanism of SF have been described. Thus, we performed in vitro study to investigate if treatment of SF affects activating transcription factor 3 (ATF3) expression and ATF3-mediated apoptosis in human colorectal cancer cells. The effects of SF on cell viability and apoptosis were measured by MTT assay and Western blot analysis against cleaved poly (ADP-ribose) polymerase (PARP). ATF3 activation induced by SF was evaluated using Western blot analysis, RT-PCR and ATF3 promoter assay. SF treatment caused decrease of cell viability and increase of apoptosis in a dose-dependent manner in HCT116 and SW480 cells. Exposure of SF activated the levels of ATF3 protein and mRNA via transcriptional regulation in HCT116 and SW480 cells. Inhibition of extracellular signal-regulated kinases (ERK) 1/2 by PD98059 and p38 by SB203580 attenuated SF-induced ATF3 expression and transcriptional activation. Ectopic ATF3 overexpression accelerated SF-induced cleavage of PARP. These findings suggest that SF-mediated apoptosis may be the result of ATF3 expression through ERK1/2 and p38-mediated transcriptional activation.

This study investigated the effect of NH4 + concentrations on microalgae growth by appling mixotrophic microalgae chlorella vulgaris in order to treat anaerobic digested food waste leachate. The growth rate and final microalgae growth were an order as 400 > 100 > 800 > 1300 mg-N/L. As results, The growth rate and final growth of microalgae were highest at ammonia concentration of 400 mg-N/L, On the other hand microalgae growth was inhibited when ammonia concentrations were over 800 mg-N/L. high concentrations of nitrogen over 800 mg-N/L interrupt the growth of microalgae. All of nitrogen and phosphorus were removed by microalgae at the ammonia concentration of 100~400 mg-N/L. In addition, when ammonia concentration was over 800 mg-N/L, the removal of nitrogen and phosphorus was limited mainly due to the microalgae growth limit. It was possible to treat anaerobic digested food waste leachate with mixotrophic microalgae when the ammonia concentration was controlled below 400 mg-N/L.

The main aim of this paper, via the method of statistical data analysis, is to analyze the current situation of the shipping industry and policies of the shipping industry in Vietnam. Section 1 gives some key information about the current situation of the shipping industry in Vietnam such as: shipping companies and their fleets, seafarers and ports. In view of the many restrictions faced by the Vietnamese shipping industry, section 2 is used to disclose the policies issued by the Vietnamese government in order to help the domestic maritime industry develop further in the near future, and the outcomes of implementing these policies. Besides the fact that some of the measures were feasible and reasonable, some others were not as effective as they were supposed to be, or in fact the initial forecast of their effectiveness was far-fetched. In the last section, after analyzing reasons for the failures, the author proposes that focusing on coastal transportation routes, fleet and port restructuring and skilled labour training were truly necessary to the development of the industry in the immediate period. Furthermore, this paper is also expected to provide a basic foundation for further research by the authors about 'the strategical approach for the development of shipping industry in developing countries: a case of Vietnam' in the near future.