Chlorine dioxide (ClO2) has recently emerged as an ideal disinfectant and has shown a wide range of antimicrobial activities in various pathogenic microorganisms. In this study, the virucidal effect of ClO2 at low concentration (0.02 ppm) and higher concentration (0.06 – 0.09 ppm) against Adenovirus and Herpesvirus was evaluated based on the NF T 72-281 and ASTM 1053-11 standard methods at different exposure times. The virus suspension was dried onto the carrier and then exposed to gaseous ClO2 (gClO2) at 22 ± 2∘C. For Adenovirus, exposure at a low concentration of ClO2 at the middle height resulted in the average log10 reduction of 0.95, 2.65, and 5.30 after 1, 3, and 6 h post-exposure (pe), respectively. Moreover, more than 4-log10 reduction was achieved at 4 and 6 h pe with higher concentrations of ClO2. On the other hand, the antiviral activity of gClO2 at the middle height was also effective against Herpesvirus. In particular, at 1 h pe, a less than 4-log10 reduction was observed at all examined concentrations of ClO2, whereas exposure for 3 and 6 h (with low concentration) or 2 h (with higher concentration) inactivated completely viruses attached to the carrier. These results suggested that ClO2 fumigation is a potential alternative method for disinfecting healthcare facilities, high-containment laboratories, and households with a safe concentration for human health.

The aim of this study was to investigate the effect of isolated lactic acid bacteria (LAB) on the quality of high moisture rye silage. Rye forage (Secale cereale L.) was harvested at the heading stage (27.3% of dry matter (DM)) and cut into approximately 3-5 cm lengths. Then, the forage divided into 4 treatments with different inoculants: 1) No additives (CON); 2) Lactobacillus brevis strain 100D8 at a 1.2 x 105 colony-forming unit (cfu)/g of fresh forage (LBR); 3) Leuconostoc holzapfelii strain 5H4 at a 1.0 x 105 cfu/g of fresh forage (LHO); and 4) Mixture of LBR and LHO (1:1 ratio) applied at a 1.0 x 105 cfu/g of fresh forage (MIX). About 3 kg of forage from each treatment was ensiled into a 20 L mini-bucket silo in quadruplicate for 100 days. After silo opening, silage was collected for analyses of chemical compositions, in vitro nutrient digestibilities, fermentation characteristics, and microbial enumerations. The CON silage had the highest concentrations of neutral detergent fiber and acid detergent fiber (p = 0.006; p = 0.008) and a lowest in vitro DM digestibility (p < 0.001). The pH was highest in CON silage, while lowest in LBR and MIX silages (p < 0.001). The concentrations of ammonia-N, lactate, and acetate were highest in LBR silage (p = 0.008; p < 0.001; p < 0.001). Propionate and butyrate concentrations were highest in CON silage (p = 0.004; p < 0.001). The LAB and yeast counts were higher in CON and LHO silages compare to LBR and MIX silages (p < 0.001). However, the mold did not detect in all treatments. Therefore, this study could conclude that L. brevis 100D8 and Leu. holzapfelii strain 5H4 can improve the digestibility and anti-fungal activity of high moisture rye silage.

Purpose: Researchers have shown that aesthetic judgments of artworks depend on contexts, such as the authenticity of an artwork (Newman & Bloom, 2011) and an artwork’s location of display (Kirk et al., 2009; Silveira et al., 2015). The present study aims to examine whether contextual information related to the creator, such as whether an artwork was created by a human or artificial intelligence (AI), influences viewers’ preference judgments of an artwork. Methods: Images of Impressionist landscape paintings were selected as human-made artworks. AI-made artwork stimuli were created using Google’s Deep Dream Generator by mimicking the Impressionist style via deep learning algorithms. Participants performed a preference rating task on each of the 108 artwork stimuli accompanied by one of the two creator labels. After this task, an art experience questionnaire (AEQ) was given to participants to examine whether individual differences in art experience influence their preference judgments. Results: Setting AEQ scores as a covariate in a two-way ANCOVA analysis, the stimuli with the human-made context were preferred over the stimuli with the AI-made context. Regarding the types of stimuli, the viewers preferred AI-made stimuli to human-made stimuli. There was no interaction effect between the two factors. Conclusion: These results suggest that preferences for visual artworks are influenced by the contextual information of the creator when the individual differences in art experience are controlled.

The purpose of this study was to examine the impacts of AI-integrated MALL on Korean students’ TOEIC preparation, by comparing with AI-integrated CALL. A total of 496 freshmen students participated in this study. They were arbitrarily assigned to AI CALL (n = 190), AI MALL (n = 164), and the control (n = 132) groups. During a 2021 academic semester, the two experimental groups studied TOEIC through computers or mobile phones, integrated with AI technology. The control group was taught by a human teacher, in a traditional classroom setting. Before and after the treatment, TOEIC listening and reading tests were administered. Paired samples t-tests and one-way ANOVAs, were used to analyze collected data. Findings show that all groups significantly increased listening and reading test scores. Group comparison results show that the AI CALL group outperformed the control group for both tests. This group also outperformed the AI MALL group for the reading test. Based on this, pedagogical implications are invaluable

최근 소프트웨어(SW) 교육의 중요성이 부각되면서 SW 교육을 위한 다양한 정책과 플랫폼이 발표되고 있다. 특히 SW 교육을 효율적으로 진행하기 위해서는 단순히 교재와 수업만으로는 한계가 있으므로, 방과후 학 교, 협동 혹은 실습수업, 캠프운영 등 다양한 시도가 이루어지고 있다. 이 중에서 학습캠프는 집중도 높은 수업, 타 학습자와의 원활한 교류, 지도 교사의 빠른 피드백 등으로 많은 주목을 받고 있는 수업 플랫폼이 다. 이 같은 추세에 발 맞추어 로봇체험캠프, 앱개발 캠프 등 다양한 캠프들도 등장하고 있다. 특히, 게임제 작 캠프는 SW교육을 처음 접하는 학생들의 흥미와 학습 동기부여를 높여주어 많은 주목을 받고 있는데, 본 연구에서는 게임전공 연구자들 중심으로 기획 된 게이미피케이션 기반 온라인, 오프라인 코딩 캠프 운영을 통해 온라인과 오프라인 캠프의 재미와 만족도를 비교하고, 각 장단점을 확인하여 게이미피케이션 기반 SW교육 캠프 콘텐츠 제작 및 프로그램 운영 시 고려 할 점들을 고찰 하고자 하였다. 또한, 사용자 인터뷰 를 통해 실제 학습자와 교사의 의견을 수렴하여 각각의 플랫폼이 가지고 있는 단점을 상호 보완하고, 장점 을 부각시키는 방안을 검토 하였다.

This study assessed the changes in the fiber properties of virgin and recovered fibers from lab-scale and pilot-scale depolymerization reactors based on the thermal air oxidation-resistance characteristics. Lab-scale and pilot-scale depolymerization reactors had different depolymerization volumes. Results showed that the lab-scale and pilot-scale peak solvent temperatures were 185 °C and 151 °C, respectively. The lab-scale had highest solvent temperature rate increase because of the small depolymerization volume and the dominant role of the cavitation volume. The structural properties of the recovered and virgin fibers were intact even after the depolymerization and after the pretreatment and oxidation-resistance test. We observed 1.213%, 1.027% and 0.842% weight loss for the recovered (lab-scale), the recovered (pilot-scale) and virgin fibers because of the removal of impurities from the surface and chemisorbed gases. Further, we observed 0.8% mass loss of the recovered fibers (lab-scale) after the oxidative-onset temperature because of the “cavitation erosion effect” from the dominant of the cavitation bubbles. The “cavitation erosion effect” was subdued because of the increased depolymerization volume in the pilot-scale reactor. Therefore, negligible impact of the pilot-scale mechanochemical recycling process on the structure and surface characteristics of the fibers and the possibility of reusing the recovered fibers recycling process were characteristic. Representative functional groups were affected by the thermal oxidation process. We conducted HPLC, HT-XRD, TGA– DSC, XPS, SEM, and AFM analysis and provided an extensive discussion of the test thereof. This study highlighted how misleading and insufficient small-lab-scale results could be in developing viable CFRP depolymerization process.

Two tree frogs, Dryophytes suweonensis and Dryophytes japonicus, inhabiting Korea, are morphologically similar and share the same habitats. Therefore, they are identified mainly through their calls, especially for males. Dryophytes suweonensis is registered as an endangered (IUCN: EN grade) and protected species in South Korea. Thus, it is necessary to develop a method to rapidly identify and discriminate the two species and establish efficient protection and restoration plans. We identified significant genetic variation between them by sequencing a maternallyinherited mitochondrial 12S ribosomal DNA region. Based on the sequence data, we designed a pair of primers containing 7 bp differences for high resolution melting (HRM) analysis to rapidly and accurately characterize their genotypes. The HRM analysis using genomic DNA showed that the melting peak for D. suweonensis was 76.4±0.06°C, whereas that of D. japonicus was 75.0±0.05°C. The differential melt curve plot further showed a distinct difference between them. We also carried out a pilot test for the application of HRM analysis based on immersing D. suweonensis in distilled water for 30 min to generate artificial environmental DNA (eDNA). The results showed 1.10-1.31°C differences in the melting peaks between the two tree frog samples. Therefore, this HRM analysis is rapid and accurate in identifying two tree frogs not only using their genomic DNA but also using highly non-invasive eDNA.

Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) is an N6-methyladenosine (m6A) RNA modification regulator and a key determinant of premRNA processing, mRNA metabolism and transportation in cells. Currently, m6A reader proteins such as hnRNPA2/B1 and YTHDF2 has functional roles in mice embryo. However, the role of hnRNPA2/B1 in porcine embryogenic development are unclear. Here, we investigated the developmental competence and mRNA expression levels in porcine parthenogenetic embryos after hnRNPA2/B1 knock-down. HhnRNPA2/B1 was localized in the nucleus during subsequent embryonic development since zygote stage. After hnRNPA2/B1 knock-down using double stranded RNA injection, blastocyst formation rate decreased than that in the control group. Moreover, hnRNPA2/B1 knock-down embryos show developmental delay after compaction. In blastocyste stage, total cell number was decreased. Interestingly, gene expression patterns revealed that transcription of Pou5f1, Sox2, TRFP2C, Cdx2 and PARD6B decreased without changing the junction protein, ZO1, OCLN, and CDH1. Thus, hnRNPA2/B1 is necessary for porcine early embryo development by regulating gene expression through epigenetic RNA modification.

To assist farmers in shifting from subsistence to commercial agriculture, the government of Laos has promoted farmer organizations throughout the country. The farmer production groups have gradually transformed and emerged into strong cooperatives or associations. The purpose of this study was to examine the factors affecting farmers’ participation in FPGs. Data were collected from primary and secondary sources. A total of 140 respondents participated in a field survey in three kumb ban (village groups) in the Xepon District. Descriptive statistics and binary logistics regression models were used to analyze the data. The results show that the education level of household head, farmland size, access to credit, and perceived trust for farmer production group membership are significantly different from those who are non-members of the groups. However, non-members of FPGs face more constrain in accessing the market, as compared to the members of FPGs. The empirical results from the Binary Logit Model indicate that education level of headed household, farmland size, access to credit, perceived trust, and constraint to market are factors that significantly determine farmers’ participation in FPGs. This study contributes to the empirical literature by providing a better understanding of farmer participation in the groups. The government of Laos should consider and promote access to credit, farmer learning, and market integration to improve farmers' participation in FPGs.

The purpose of this study is to determine whether the use of artificial intelligence (AI) improves English listening and reading proficiency. It focuses on whether its impacts differ by gender and major. The study was conducted in 2020 with 340 freshmen students in Korea. There were 182 male and 208 female students. The participants majored in different areas: 139 students majoring in aeronautics, 130 students studying policing and safety, 121 students studying in the arts department. The TOEIC test was adopted as pre- and post-tests to explore the impacts of using AI on English learning. To analyze the data, two-way ANOVAs were administered. The study found no interaction effects of gender or major on either listening and reading proficiency. However, gender and major, respectively, had significant impacts on listening. Regarding reading proficiency, major alone affected the students’ performance. Based on these findings, pedagogical implications are considered.