This study was carried out to determine the factors on achieving good viability of embryos biopsied fur sexing, to investigate pregnancy rate following embryo transfer(ET) with sexed embryos, and to confirm the accuracy for the calves bort following ET with sexed embryos by polymerase chain reaction(PCR). To investigate viability of Hanwoo embryos after biopsy for sexing, fresh and frozen/thawed embryos were biopsied according to different developmental day of blastocysts, different stage of blastocysts, and different biopsy grade and the embryos themselves were incubated for 2 hours in TCM199 after microsection to be evaluated morphologically for recovery as blastocyst. The results obtained were as follows : 1. The rate of oocytes cleaved in vitro and the rate of blastocyst of the cleaved oocytes were 52.5% and 21.6%, respectively. The rate of blastocyst on day 8 was 11.2%, denoting the highest rate during whole culture period posterior to in vitro fertilization(IVF) 2. After biopsy for sexing, the viability rate of blastocyst on day 7, 8 and 9 was 75.0%, 88.4%, and 100.0%, respectively and the viability of early, mid, and expanded blastocyst after biopsy was 75.0%, 88.9%, and 91.1%, respectively The viability rate of fresh and frozen/thawed embryos was 89.9%, 71.4%, respectively. And the viability of expanded, hatching, and hatched blastocyst of frozen/thawed embryos was : 75.0%, 75.0%, and 50.0%, respectively. The viability of embryos according to biopsy grade of 10∼20%, 21∼30%, and 31∼40% was 85.7%, 91.5%, and 71.4%, respectively. 3. Pregnancy rate after transfer with biopsied embryo between flesh and frozen/thawed embryos was 22.6% and 20.0%, respectively. 4. In comparison between sex by PCR method and sex of calves born after embryo transfer, the accuracy of sex deterimination was 92.3% (12/13).

With a view to investigating how Epimedium koreanum Nakai affects formation of melanin content in skin, we have measured change in the number of cell, melanin content, and tyrosinase activity by treating its aqueous extracts at various concentrations with B16 melanoma cells, the result of which is as follows: Firstly, cell viability has been shown as over 95% when treating with the aqueous extract of Epimedium koreanum Nakai up to 200 ug/mL, from which the effect of cell toxicology has not nearly appeared. Secondly, the number of cell has been slightly decreased than that of the control group when treating with the aqueous extract of Epimedium koreanum Nakai, which has not shown a statistical noteworthiness. Thirdly, the aqueous extract of Epimedium koreanum Nakai has increased in tyrosinase activity. Fourthly, the aqueous extract of Epimedium koreanum Nakai has promoted the formation of melanin content, its final product. According to the result, it is confirmed that Epimedium koreanum Nakai has the influence on promoting melanization of B16 melanoma cell, and it is considered that Epimedium koreanum Nakai can be applicable for curing vitiligo caused by decrease or loss of pigment through a systematic study.

본 연구는 조경적 측면에서의 모법인 대한민국 헌법의 여러 조문 중에서 환경보전과 생태학이라는 객체에 직, 간접적으로 관련되어 있다고 판단되는 조문들을 자연과학적 관점에서 분석했으묘, 현재 적용되고 있는 환경보전과 생태학과 관련된 국내법(법률: 40종, 지침: 1종, 판례: 1종)과 국외법(헌법: 1종, 법률: 34종, 조례: 2종, 판례: 3종)을 수집, 비교하였다. 또한 수집된 국내, 외 관련볍규를 법조문상의 내용을 기준으로 하여 환경(생태)보전의 기본원칙 및 개념, 계획수립과 사업시행, 유형, 정책 및 관련사업, 관련권리(권한) 및 의무(책임), 허용 및 규제행위, 환경영향평가 및 행정절차, 제도 등의 항목별로 분류하여 비교, 분석해 봄으로써 여러 법률에 산재해 있는 환경보전과 생태학 관련법규를 일목요연하게 조합하여 상호 관련성을 고찰해 보고, 외국의 각국들이 어떻게 환경보전과 생태학 관련 법규정들을 타 분야로부터 독립시켜 왔으며, 환경적인 측면에서 선진화된 사업을 해오고 있는가를 검토해 보았다.

The main goal of this research was to develop a Golf course planning Methodology in terms of use and preservation of natural resources which consider balance of Ecosystem. This study suggested the necessity of analysis of natural resources in the development site prior to its planning. The analysis will give a benefits both the profits of private enterprise and national interests for reuse of natural resources. Study site was planned region of Kyungpook Country Club, which located near Weagwan. In connection with this study, natural resources which will reuse in the planning stage and soil status in the development site were analyzed.

The purpose of this study is to establish more rational and practical planning theory for amusementpark. It analyze and considerate the fluctuation of the people who come and use a Tong-Ch'on amusementpark. The results drawn from this research work are as follows ; There are considerable correlation between use fluctuation and some factors. The factors are season (spring, summer, autumn) as a time, temperature, cloud amount, duration of sunshine, weather (rainy day) as a climate and date (weekday, holiday) as a social system. The important variables are temperature, cloud amount, duration of sunshine and date (week day, holiday) to estimate the user of amusementpark. I can reduce the following two types of regression models. 1. () 2. () Y ; Number of User TEM ; Temperature CLO ; Amount of cloud SUN ; Duration of Sunshine WI ; Weekday W3 ; Holiday Those model is inorder to estimate the user for management of Tong-Ch'on amusementpark and use on the computation of facility size for reconstruction. Besides the amusementpark, city park and outdoor recreation area could estimate of user throuth this method. But, I am not sure about the regression models because I didn't apply the regression models to the other amusementpark, city park or outdoor recreation area. Therefore, I think that this problem needs to study in the future.

This is a study on a landscape planning along the street 1~4, which are gateways to Taegu city and an important scenery. Therefore, emphasis was given to provide a bright, clean and beautiful first impression to visitors and citizens. The area is being developed for education and athletic park. Plans to construct a fountain, pergolas and a pond were set up to complement the active and passive facilities to the existing a athletic facilities in the area. Since D part in the area have alkali soil (pH 8.1), soil amendment is necessary for successful growth of trees and ground covers. When the area is filled or added with new soil for soil amendment, it will be important to use fresh soil which can support plant growth. It will be ideal to use ground covers and shrubs on the existing retaining walls along the street for keeping natural vegetation and slope stability instead of further cutting and reconstruction. It is not reasonable in technical and economic sense to remove rocks of the slope for planting trees. In order to harmonize plant color distribute, tall needle and broad leaf trees in the background as a screen, flowering trees were arranged in groups at front, and annuals were mass-planted along the footway. Natural-form-trees will be planted because they not only serve as a greenbelt but also are more economics in maintenance than formative trees.

국가 전략적 차원에서 전체 사회기반시설물에 대해 미래에 필요한 유지보수 예산 파악하는 것은 매우 중요한 부분이다. 그러나 현 재 국내에서는 전체 대규모 시설물에 적용할 수 있는 유지보수비용 예측 기법이 거의 전무한 실정이다. 본 연구에서는 현재 국내에서 가용한 자 료와 적용 가능한 예측모델을 토대로 우리나라 전체 향후 SOC 시설물에 대한 향후 유지보수 투자 규모를 예측하였다. 실제 지출비용과 비교하 여 가장 적합한 예측모델을 선정하고 관리주체에서 과거에 지출한 유지보수비용 자료를 이용하여 일부 모델은 개발하여 교량, 터널, 포장, 항 만, 댐, 공항, 상수도, 하천, 항만 등의 사회기반시설물에 대한 최적의 미래 유지보수 비용을 산출하였다. 앞으로 10년간 총 비용예측결과, 8종의 사회기반시설물에 대하여 약 23조원이 필요한 것으로 나타났으며, 가장 비용이 크게 발생하는 시설물은 도로포장, 도로교량으로 나타났다.

In this study, we analyzed the effect of the drainage slope to collapse. The presence of drainage has a low correlation with the collapse of the slope. But drainage of the drainage has an important role in preventing the decay slope. Therefore, the drain state is very important in order to evaluate the drainage.

In this study, weight of assessment items for rock and soil slopes is deducted using AHP(Analytic Hierarchy Process). And also, weight of each performance for final evaluation is analyzed. As a result, tension crack and ground deformation make up a large proportion in all slopes, and weight of each performance based on the location of the slope does not show a big difference.

Since the ground subsidence frequently occurs caused by water and sewage pipeline leakage, this paper reviews current maintenance management regulation, legislation related to water and sewage pipeline measurement for maintenance in practice to prevent and manage this phenomenon. Analysis of current system shows the difficulty of ground subsidence evaluation and importance of the law which is now being established has been emerged. In addition, it is determined that the regulation contains specified content with specific range of water and sewage pipeline is required for its maintenance.

Recently, as ‘ground subsidence in urban area’ problem become social issue, Central & Local Government examined current state of ground subsidence. As a results, It is revealed that 1)sewage pipeline damage, 2)excavation work of surrounding pipeline, and 3)water pipeline damage were main reason of subsidence. This study is going to present weak-point for each type of pipeline in the case of water pipeline monitoring, by analyzing current state materials of water pipeline leakage work of ○○city, to prevent ground subsidence from water pipeline leakage.

The Characteristics of water catchment terrain on the cut-slopes of general nation road was analyzed. As the water catchment terrain increased the frequency defects on slopes are increased, and it shows a linear increase pattern. As a results of analyzing the location of water catchment terrain, when the intersection line of water catchment terrain located in the slopes the frequency defects were higher then it located top of slopes. also the types of defects were different.

Evaluation criteria for rockfall is proposed using 137 real data. Rockfall data was classified 3 categories considering damage level of rockfall protection fence, and rockfall energy for each was made a calculation. In results, 50kJ, 70kJ and 117kJ of rockfall energy are proposed as evaluation criteria.

The change of paradigm to performance-Based evaluation system for maintenance of SOC infrastructure is the effective way to invest limited financial. Selecting of the evaluation items is very important to develop a comprehensive performance assessment techniques and it determined by Delphi analysis. Also, to analyze the importance of the performance items, the AHP analysis was performed.

Evaluation items were deducted by based on literature materials to supplement the evaluation system of rock cut-slope in national road. Delphi survey of experts were conducted to review the final evaluation items. As a result of reviewing the significance through stepwise multiple linear regression, all of deducted items were statistically significant because these had lower P-value than 0.05. And It was confirmed that the items were selected appropriately as they had relatively similar levels of the weight.

Currently internal harbor and fishing port facilities are being maintained with the "Safety inspection and in-depth safety inspection detailed guideline" This is provided with the evaluation focusing on the safety and durability of the structure, based on the usability are not presented. In this study, we derive the evaluation items of the harbor and fishing port facilities for the transition from the maintenance of the existing system to the performance-based safety and maintenance system.