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        검색결과 163

        62.
        2013.09 KCI 등재 서비스 종료(열람 제한)
        본 연구는 레시틴으로 나노입자화 시킨 티아민 디라우릴 설페이트의 향장활성 증진에 관한 것이다. TDS를 포집시킨 나노입자는 150 ~ 200 nm의 크기를 나타내는 구형이며, 또한 제타포텐셜을 측정하여 여러 pH범위에서 안정한 것을 확인하였다. TDS 나노입자는 인간 섬유아세포(CCD-986sk)에 높은 농도를 처리하여도85%의 세포생존률을 보였다. 자유라디칼소거활성 실험을 진행한 결과 나노입자화하지 않은 TDS 희석액(1.0mg/mL)은 81.6%의 활성을 나타내었고, 나노입자화한 TDS 용액은 이보다 더 높은 88.1%의 높은 라디칼 소거활성을 보였다. TDS 나노입자는 자외선을 조사시킨 CCD-986sk에서 MMP-1의 발현을 41.4% 감소시켰다.TDS 용액과 TDS 나노입자를 가지고 salmonella typhimurium, listeria monocytogenes에 대하여 항균활성을 측정하였다. TDS 나노입자의 경우 양성대조군의 항균활성과 비슷한 결과를 나타내었다. 이러한 결과들로TDS 나노입자가 항산화, 미백, 주름개선 효능같은 향장 소재로서의 적용이 가능할 것이라 생각된다.
        63.
        2013.06 KCI 등재 서비스 종료(열람 제한)
        In general, stepwise hot steaming process is known to be effective in improving its biological activities; however, not much employed in processing Codonopsis lanceolata due to its hardness. The complex processed C. lanceolata showed highest free radical scavenging acitivity as 45.21%. Total phenol and flavonoid content were of complex processed C. lanceolata higher than conventional extract and alone steaming process. It was showed the lower melanogenesis rate on melanin production test by B16F10 cells as 27.46%. High inhibitory of tyrosinase was also measured as 28.61% by adding steamed Codonopsis lanceolata extracts by high pressure extraction of 1.0 mg/ml. And anti-wrinkle activity were 39.08%. In comparing phenolic acids profiles in the extract, in general higher amounts of polyphenol were obtained possibly by easy release of active components during thermal processing, which results in better antioxidant activities than that of general extract. This findings can also be supported by result that the extract by steaming process showed better activities than the general extraction extract.
        67.
        2013.04 KCI 등재 서비스 종료(열람 제한)
        This study was performed to investigate the enhancement of immunomodulatory activities of Lithospermum erythrorhizon by extreme process. The extracts are WE100 (water extract for 24 hours at 100℃), WE80 (water extract for 24 hours at 80℃), EE (70% ethyl alcohol extract for 24 hours at 80℃) and EPE (extreme process for 30 minutes at 25℃, 500 MPa after 70% ethyl alcohol extracts for 3 hours at 40, 50, 60℃). Extraction yield was increased up to 5~10% by extreme process, compare to the normal extraction such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts was showed in the range of 12.68~15.89% at 1.0mg/ml for human lung cell (HEL299). The EPE40 was showed the lowest cytotoxicity 12.68%. The EPE60 extracted by extreme process increased the growth of human B and T cells up to 12.12×104 cells/ml and 14.88×104 cells/ml, respectively and the EPE60 greatly increased the cytokine secretion of both IL-6 and TNF-α. The extracts by extreme process also exhibited higher levels of nitric oxide production from macrophages than the lipopolysaccaharides. It can be concluded that Lithospermum erythrorhizon has immune activities and The extreme process could increase higher immune activities possibly by immunomodulatory compounds.
        68.
        2013.02 KCI 등재 서비스 종료(열람 제한)
        According to previous reports, antioxidant activities of Codonopsis lanceolata could be increased by a steaming process. This study was performed to improve its antioxidant activity and skin whitening activities of C. lanceolata by high pressure and stepwise steaming complex process. The complex processed C. lanceolata showed highest free radical scavenging acitivity as 45.21%, and for phenol and flavonoid contents, complex processed C. lanceolata contained higher than those from conventional extraction process or steaming process alone. The Cytotoxicity of all C. lanceolata extracts also showed low cytotoxicity against human fibroblast cell (CCD-986sk) as 4.49 ~ 10.40%. In whitening activity, high inhibition of tyrosinase activity was estimated as 25.08% by adding the extracts from complex process. We found that whitening and antioxidant activity of complex processed C. lancolata extract was higher than those obtained from conventional extraction and a steaming process because various kinds of antioxidant compounds could be easily released by combined process, compared to one of each process.
        69.
        2012.08 KCI 등재 서비스 종료(열람 제한)
        This study was performed to enhance contents of low molecular weight ginsenoside Rh2 and Rg3 using an ultra high pressure and steaming process in wild cultured-Root in wild ginseng. For selective increase in contents of Rg3 and Rh2 in cultured wild ginseng roots, an ultra high extraction was applied at 500MPa for 20 min which was followed by steaming process at 90℃ for 12 hr. It was revealed that contents of ginsenosides, Rb1, Rb2, Rc and Rd, were decreased with the complex process described above, whereas contents of ginsenoside Rh2 and Rg3 were increased up to 4.918 mg/g and 6.115 mg/g, respectively. In addition, concentration of benzo[α]pyrene in extracts of the cultured wild ginseng roots treated by the complex process was 0.64 ppm but it was 0.78 ppm when it was treated with the steaming process. From the results, it was strongly suggested that low molecular weight ginsenosides, Rh2 and Rg3, are converted from Rb1, Rb2, Rc, and Rd which are easily broken down by an ultra high pressure and steaming process. This results indicate that an ultra high pressure and steaming process can selectively increase in contents of Rg3 and Rh2 in cultured wild ginseng roots and this process might enhance the utilization and values of cultured wild ginseng roots.
        70.
        2012.08 KCI 등재 서비스 종료(열람 제한)
        In general, stepwise hot steaming process is known to be effective in improving its biological activities; however, not much employed in processing Codonopsis lanceolata due to its hardness. In this study, C. lanceolata was first pretreated with warm water at 50℃ and 60℃ for two hours, then steamed for 3 hours. Antioxidant activities of 70% ethanol extracts were compared with the extract from the water solvent: 41.58% vs 8.98% of DPPH radical scavenging activity in adding 1.25mg/ml of steamed extract and water extract, respectively. Reducing power of steamed and fresh C. lanceolata were also measured as 1.39 and 0.71. Total poly phenolic of the steamed extract was estimated as 12.11mg/g, compared to 3.98mg/g fresh C. lanceolata. Total flavonoid contents were also obtained as 11.48mg/g, compared to 7.11mg/g of fresh C. lanceolata. In comparing phenolic acids profiles in the extract, in general higher amounts of gallic acid, trans-ferulic acid, vanillic acid were obtained possibly by easy release of active components during thermal processing, which results in better antioxidant activities than that of water extract. This findings can also be supported by result that the ethanol extract showed better activities than the water extract.
        71.
        2012.04 KCI 등재 서비스 종료(열람 제한)
        This study compared the contents of low molecular ginsenoside according to fermentation process in low grade fresh ginseng. Low grade fresh ginseng was directly inoculated with a 24 h seed culture of Bifidobacterium Longum B6., Lactobacillus casei., and incubated at 36℃ for 72 h. Bifidobacterium Longum B6 was specifically was found to show the best growth on 3,255×106 CFU/ml after 48 h of fermentation. The content of ginsenoside Rb1, Re and Rd were decreased with the fermentation but ginsenoside Rh2 and Rg2 increased after fermentation process. In the case of low molecular ginsenoside conversion yields were 56.07% of Rh2, 12.03% of Rg3 and 77.11% of Rg2, respectively. In addition, compound-K was irregular conversion yield as long as 72 h of fermentation. This results indicate that fermentation process could increase the low molecular ginsenoside in low grade fresh ginseng.
        72.
        2011.12 KCI 등재 서비스 종료(열람 제한)
        The bark of the root and stem of Ulmus davidiana var. japonica has been used as a traditional Korean medicine to treat inflammatory disorders. This plant reportedly shows antioxidant, anticancer, and anti-inflammatory effects. In this study, we investigated the protective effects of Ulmus davidiana var. japonica ethanolic extract (UDE) on UVB irradiation-induced wrinkle in hairless mice. We evaluated for their free radical-scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the anti-elastase activities, and for their anti-matrix metalloproteinase-1 (MMP-1) activity in human skin fibroblast cells. In the wrinkle measurement and image analysis of skin replicas, the results showed that UDE significantly inhibited wrinkle formation caused by chronic UVB irradiation. These results suggest that UDE has anti-wrinkle activity.
        73.
        2011.12 KCI 등재 서비스 종료(열람 제한)
        Conventional Thiamine Dilauryl Sulfate (TDS) powder has a low stability. In order to solve this problem, this study was performed to improve the solubility of TDS. The process for enhance solubility of TDS was nano grinding mill and ultrasonic dispersion process. TDS paticle was manufactured to nano size through nano grinding mill process. The size of TDS nanoparticle was measured as average 220 nm by DLS. And The TDS nanoparticle in water solution manufactured through ultrasonic dispersion process. The TDS nanoparticle in water solution was showed the highest solubility with 40% ethanol. These results was increased the concentration of TDS from 200 ppm to 240 ppm in water solution. The TDS nanoparticle in water solution showed diameter of Colletotrichum gloeosporioides growth with smaller than about 1.56 cm compared to the TDS paticle in water solution at same concentration. Also, TDS nanoparticle in water solution showed growth inhibition activity as 59.2% with higher than about 10% compared to the TDS paticle water solution in same concentration. Finally, TDS nanoparticle in water solution was increased solubility through nano grinding mill and ultrasonic dispersion process. Also, the increase of concentration in TDS nanopaticle in water solution according to solubility enhancement lead to an result enhancement of antifungal activity. Consequently, we suggested that the TDS nanoparticle in water solution was more effective than TDS particle in water solution owing to the sub-cellular particle size, ability to persistence and targeting to cell membrane of Colletotrichum gloeosporioides. Furthermore we expected the applicating possibility with bio pesticide.
        74.
        2011.08 KCI 등재 서비스 종료(열람 제한)
        In this study, we investigated the cosmetic application of Acer mono sap through an ultra-high pressure process. Exposing Acer mono sap to a ultra-high pressure process resulted in 90.1% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration. Acer mono sap also showed the hightest free radical scavenging activity after the ultra high pressure process. The melanogenesis inhibition rate in cloned M-3 cells was 59.0%. Tyrosinase was inhibited at a rate of 87.2% by adding 100% HPAMS. Anti-wrinkle activity was 78.1%. Acer mono sap showed enhanced storage following the ultra high pressure process. These results indicate that Acer mono sap may be a source for functional cosmetic agents capable of improving antioxidant, whitening, and antiwrinkling effects.
        75.
        2011.06 KCI 등재 서비스 종료(열람 제한)
        This study was performed to enhance antifungal activity of anthracnose in chili pepper by nanopaticles of thiamine di-lauryl sulfate (TDS) through high pressure homogenization process. Yield of TDS was 79.14% by reaction of thiamine hydrochloride and sodium lauryl sulfate. TDS nanopaticle solution was manufactured through high pressure homogenization process. The turbidity of nanoparticles solution was increased with increasing the concentration of TDS, and nanoparticles solution of 100 ppm was showed the highest turbidity with absorbance of 3.212. The size of nanoparticles solution was measured as average 258.6 nm by DLS. Nanoparticles solution of 100 ppm showed growth inhibition activity with higher than about 80% compared to the control group against Colletotrichum gloeosporioides. Finally, nanoparticles solution was increased effectively the penetration of the TDS nanopaticles on attached cell membrane of hyphae and started to destruct the cells under microscope observation. Consequently, we suggested that the TDS nanoparticle solution by high pressure homogenization process might be suitable biochemical pesticides for improving the antifungal activities against anthracnose in pepper.
        76.
        2011.06 KCI 등재 서비스 종료(열람 제한)
        In this study, we investigated antioxidant activities and whitening effects of Acer mono sap by encapsulation of nanoparticles. Acer mono sap was through ultra high pressure process and then encapsulated by lecithin. Nano-encapsulated The nanoparticles of Acer mono sap showed highest free radical scavengering effect as 89.7% in adding sample (1.0 mg/ml), compared to sap of non-encapsulation. It was showed strong inhibition effect on melanin production test by Clone M-3 cells as 47.8%. High inhibitory of tyrosinase was also measured as 85.8% by adding lecithin nano-particle of 1.0 mg/ml. The nano-particles also showed 14.8% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. These results indicate that Acer mono sap may be a source of cosmetic agents capable of improving whitening effect and antioxidant activites.
        77.
        2011.04 KCI 등재 서비스 종료(열람 제한)
        This study was to investigate the effect of fermentation extracts on the concentration of serotonin and melatonin in the serum of the ICR mice. The ICR mice were divided into water control group, lactobacillus fermentation solution including (Lactobacillus paracasei and Bifidobacterium longum B6) control group, positive control group (milk and doxylamine succinate), negative control group (caffein) and the groups treated with the extracts of Berberis koreana bark (WE: water extracts, FE-L.P: fermentation extracts of Lactobacillus paracasei, FE-B.L: fermentation extracts of Bifidobacterium longum B6). After ten-day feeding treatment, the mean concentration of serotonin for water control, WE, FE-L.P and FEB. L group was 134.72, 183.01, 232.09 and 223.78 ng/ml, respectively. The mean concentration for FE-L.P and FE-B.L group were approximately 66% larger than that for water control group. The mean concentration of melatonin for water control, WE, FE-L.P and FE-B.L group was 76.92, 106.66, 157.56 and 141.81pg/ml, respectively. The mean concentration of melatonin for FE-L.P and FE-B.L group were also larger than that for water control group. Our results indicated that the fermentation extracts of Berberis koreana bark have relatively greater potential to induce secretion of serotonin and melatonin. Therefore, the fermentation extracts have antidepressant effect.
        78.
        2011.04 KCI 등재 서비스 종료(열람 제한)
        The composition analysis and electron donating activity of 4 kinds of Korean wild sannamuls[Cacalia firma Komar. (CFK), Aster scaber Thunb. (AST), Ligularia fischeri (Ledeb.) Turcz. (LFT), Synurus palmatopinnatifidus (Makino) Kitam., SDN)] were investigated. The Korean edible mountainous vegetable was named a sannamul or chwinamul in Korea, generally. Most of them, the characterization of the each vegetable was reported, individually without comparative research. This study was compared the proximate compositions, mineral, dietary fiber and electron donating abilities on 2,2-diphenyl-1-picrylhydrazyl (DPPH) of sannamuls. In the chemical analysis of sannamuls, CFK was shown higher value in ash (5.4±0.18%), crude fat (2.5 ±0.14%), carbohydrate (8.1±0.21%), potassium (475.45±0.34mg) and magnesium (64.70±0.48mg) than others. Each mineral of the 3 other samples was a similar value but phosphorus in LFT was contained 62.91±0.43%. The compositions of total dietary fiber of the samples were listed CFK LFT SDN AST and the RC50 Value on DPPH radicals, LFK CFT AST SDN. These results have susggest that CFK and LFT may be useful for a funtional material to food development.
        79.
        2011.04 KCI 등재 서비스 종료(열람 제한)
        This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts (1.0μg/ml) from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding 1.0μg/ml of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.
        80.
        2011.04 KCI 등재 서비스 종료(열람 제한)
        In this study, whitening activity of Lithospermum erythrorhizon extracts were investigated according to several extraction processes: water extraction at 100℃ (WE100) and 60℃ (WE60), 70% ethyl alcohol extraction (EE) and ultra high pressure extraction (HPE) at 500 MPa for 30 minutes at 60℃. The extracts from ultra high pressure extraction showed the highest tyrosinase inhibition and melanogenesis inhibition activities as 52% and 79.5%, respectively, in adding 1mg/ml than others extraction processes. HPE extracts also showed the strong reducing power as 3.19 that absorbance at 450 mm. The contents of polyphenol in WE100, we measured as 10.1μg/ml in adding 1mg/ml. Extracts have a high total flavonoid contents by HPE as 4.1μg/ml at 1mg/ml. We can conclude that better whitening activity of extracts from high pressure extraction was due to high antioxidant activities which could be extracted by higher polyphenol and flavonoid contents in HPE than others.
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