This study investigated the effect of coated feed using mixed oil on rumen fermentation characteristics. Two experiments were conducted based on materials that were mixed. First, cashew nut and soybean oils were mixed with white mineral oil. And second, different plant extracts were mixed with white mineral oil. At first experiment, inclusion levels of mixed oil on diet (0.03%, 0.1%, 0.5%) were applied as variables. A coated diet was fermented with rumen inoculum according to in vitro rumen fermentation and its parameters were investigated. In the result of first experiment, no negative effects on rumen pH were found. Significantly decreased dry matter digestibility was detected at 0.5% treatment (P<0.05). Total gas productions in control and 0.03% were significantly greater than those of others (P<0.05). Significantly reduced methane productions were found in all treatments compared to the control (P<0.05). Inclusion of mixed oil did not affect on ammonia production. Total volatile fatty acid production was also not influenced by coating with mixed oils. Rumen fermentation parameters were greatly changed according to introduced plant extracts at second experiment. The significantly lowest and greatest ammonia productions were found at treatments with Ixeris dentata and Plantago asiatica, respectively (P<0.05). The significantly greatest acetate and propionate productions were detected at treatments with Crucuma longa and Zizyphus vulgaris 1, respectively (P<0.05). All treatments, except Chrysanthemum idicum, Euyale ferox seed, Moringa leaf and fruit and Zizyphus vulgaris 1, showed significantly increased total volatile fatty acid production compared to the control (P<0.05). Only Paeonia lactiflora showed significantly lesser gas production than the control (P<0.05). In methane production, Ceramium, Zizyphus, Paeonia, Agrimonia, Torilis, Mugwort, Foeniculum, Euphorbia, Taraxacum, Artemisia, Momordica, Curcuma and Moringa reduced methane significantly compared to the control (P<0.05).

본 연구는 반추위 메탄저감용 약용식물 첨가제 개발을 위해 유산균을 이용하여 발효한 두충 및 감초 추출물의 항균활성, 항산화활성 및 in vitro 반추위 발효시험을 실시하였다. 발효 약용식물 추출물 제조를 위해 접종된 종균의 성장효율을 조사하기 위해 실시한 생균수 측정 결과 L. curvatus NJ40, L. brevis NJ42 및 L. plantarum NJ45가 두충과 감초 모두에서 유의적으로 높은 균주 성장을 나타냈다(p<0.05). 항균활성측정 결과는 두충과 감초 추출물에서 공통적으로 L. curvatus NJ40 및 L. plantarum NJ45 로 발효한 추출물이 일부 병원균에 대한 항균효과를 나타내는 것으로 조사되었다. In vitro 반추위 발효시험에서 두충 및 감초 발효 추출물을 적용한 결과, 감초 추출물에서 메탄 저감효과가 나타났다. 특히 반추위내 미생물 발효 특성을 대표할 수 있는 휘발성지방산 생성효율을 향상 시키면서, 전반적 반추위 발효에 부정적 영향이 없는 것으로 나타났다.

본 연구는 천연물질에서 유래한 반추위 메탄저감용 친환경 첨가제 개발을 위해 각기 다른 종균을 이용하여 발효한 세신 추출물의 항균활성, 항산화활성 및 in vitro 반추위 발효시험을 체계적으로 실시하였다. 접종된 균주들의 성장효율을 알아보기 위해 실시한 생균수측정 결과 L. curvatus NJ40 균주에서 유의적으로 높은 균주성장을 나타냈다(p<0.05). 항균활성측정 결과는 대조구 대비 L. curvatus NJ40 및 L. plantarum NJ45 균주와 발효된 세신 추출물이 병원균에 대한 항균효과를 나타내는 것으로 조사되었다. In vitro 반추위 발효실험에 세신 및 발효 세신 추출물을 적용한 결과, 휘발성 지방산 생성량 대비 반추위 메탄 저감효과가 나타났다. 특히 반추위 미생물 활력 및 사료이용 효율을 대표할 수 있는 휘발성 지방산 생성효율에 대한 부정적 효과 없이, 오히려 휘발성지방산 생성효율을 향상시키면서 반추위 메탄 저감효과를 가져올 수 있는 것으로 나타났다.

Miscanthus sacchariflorus var. No. 1 has been newly developed in Korea. This study was conducted to assess the feed value of M. sacchariflorus var. No. 1 at different growth and harvesting time. Total 3 different miscanthus - 1y4m (first shoot and harvested at 4 month), 2y4m (second shoot and harvested at 4 month) and 2y8m (second shoot and harvested at 8 month). Two experiments were carried out, In vitro rumen simulated fermentation and In situ dry matter digestibility (DMD). Ruminal pH at in vitro fermentation were higher in M. sacchariflorus var. No. 1 treatments compared to the rice straw (RS). In volatile fatty acid production, 1y4m resulted in higher acetate production than the other M. sacchariflorus var. No. 1 at higher maturity stages. Significant differences among treatments were observed in propionate and total volatile fatty acid (VFA) productions at 9, 24 and 48 h of incubation times. Higher ammonia nitrogen productions were found as increased maturity of M. sacchariflorus var. No. 1. At In situ experiment, high DMD was detected in the order of RS (60.51%) > 1y4m (57.65%) > 2y4m (57.63%) > 2y8m (46.28%). The results from this study indicate that young and early harvested M. sacchariflorus var. No. 1 are able to improve its nutrient values in the ruminant animal.

Recently, the use of antibiotics to improve animal productivity in livestock industry was strictly restricted. For these reason, probiotics have been regarded as one of promising materials for an antibiotic alternative. In this study, we investigated how the probiotics influences on the performance of broiler chicken via meta-analysis. Eighteen researches from 1997 to 2010 were used for meta-analysis. The standard summary effects were calculated via fixed effect model and random effect model (Borenstein et al., 2009). Heterogeneity was calculated by using the Cochran’s Q statistics (Kook et al., 2009) and publication bias was calculated via Egger’s regression (Lee et al., 2011). In fixed model average daily gain, body weight serum protein content and cecal LAB showed positive effect significantly. Feed intake, feed/gain and serum cholesterol showed significant negative effect. In serum triglyceride, negative effect was found but significance was not shown. In random model, average daily gain body weight and cecal LAB showed positive effects with significance and feed/gain and serum cholesterol represented significant negative effects. Publication bias was found only in feed/gain.

This experiment was selected a β-glucan producing mushroom strain and developed industrial media, and used to β-glucan as an alternative for antibiotics in weaned pigs. Yields of mycelial biomass and extracellular β-glucan from Youngji (Ganoderma lucidum) mushroom was 8.52g/L and 4.49g/L respectively. Also, we prepared optimum formula for mushroom cultivations. A total of 144 pigs (8.6±0.9 ㎏ average body weight, weaned 20±3 days of age) were allotted to 4 different treatment groups and replicated 4 times with 8 pigs per replicate in randomized complete block design. Treatments were T1) NC (negative control, basal diet), T2) PC (positive control, basal diet+0.255% antibiotics), T3) NC+0.2% β-glucan and T4) PC+0.2% β-glucan. The T2 and T4 treatments were significantly higher in feed efficiency by antibiotics group (p＜0.05), however, there was no significant differences in terms of average daily gain (ADG) and average daily feed intake (ADFI) during phase Ⅰ(0~14 days). In phase Ⅱ(15~28 days), Pigs fed with antibiotics and β-glucan (T4) had greater ADG than other treatments (p＜0.05), while no differences were observed in ADFI and feed efficiency. During the whole experiment period, the ADG of T4 treatment was higher than other treatments. Pigs fed with β-glucan (T3 and T4) had greater diarrhea score and moisture content than other treatments (p＜0.05). Pigs fed with β-glucan (T3 and T4) had greater moisture content than other treatments (p＜0.05). However, there was no significant differences in diarrhea score and mortality of weaned pigs. There was marginal reductions in feed cost measured feed cost per weight gain used in antibiotics and β-glucan added diet during phaseⅠ. In the second phase, the treatment supplemented with antibiotics had a significantly lower feed cost per weight gain compared to the other treatments. The results from these experiments suggests that β-glucan is likely able to improve the growth performance, and reduce feed cost although they do not have similar effects like antibiotics in weaning pigs.