말라리아는 Anopheles 모기들에 의해 전파되며, 대한민국에서는 코로나 팬데믹 기간 동안(2020~2022) 약 200~300명대의 말라리아 환자가 발생하였으나, 지난 2023년에는 그 수가 폭증해 약 800명의 환자가 발생하였다. 현재까지 모기를 방제하기 위한 가장 효율적인 수단은 살충제를 사용한 방제이나, 지속적으로 이러한 화합물에 노출된 모기 개체군은 살충제에 저항성을 갖게 되는 것으로 알려져 있다. 이에 본 연구에서는 대한민국의 주요 말라리아 발생 지역인 비무장지대 부근 및 이외 지역인 용산, 평택, 오산에서 채집을 진행하였으며, 채집된 An. sinensis에 대해서 살충제 저항성과 관련되어 있다고 알려진 유전자인 acetylcholinesterase-1(ace-1)와 voltage-gated sodium channel(vgsc) 영역에 대한 저항성 돌연변이 보유 여부를 각각 확인하였다. 실험 결과 채집된 모든 지역에서 G119S(ace-1), L1014F,C(vgsc) 돌연변이가 발견되었으며, 그 빈도는 계절과 장소에 따라 차이를 나타내었다. 본 실험 결과는 향후 말라리아 감염 억제를 위한 매개체 연구에 활용될 수 있을 것으로 기대된다.

Field surveys were conducted to collect and refine "Natural resource survey" and ecosystem research data of 23 National parks in South Korea, including Hallasan on Jeju Island and Palgongsan newly designated as national park in 2023. Among the collected data, a total of 1,538 species from 54 families of Hymenoptera insects were recorded. Within these families, the largest number of species was recorded in the family Ichneumonidae with 514 species, followed by Formicidae (118 species), Braconidae (112 species), and Tenthredinidae (135 species), exhibiting relatively high species diversity. Among the National parks, Jirisan, the first designated National park in South Korea with a relatively large area compared to other parks, recorded the highest species diversity with 618 species, while Taebaeksan recorded relatively low species diversity with 77 species. Correlation analysis between the number of Hymenoptera species and other factors revealed a statistically significant and relatively strong positive correlation (P > 0.005, R = 0.864) with the total number of insects, and a statistically significant but relatively weak positive correlation (P > 0.005, R = 0.615) with the park's area.

Pyrethroid resistance in cockroach populations has been a public health challenge since the 1950s. The pyrethroid resistance in the German cockroach, Blattella germanica, is primarily attributed to knockdown resistance (kdr) mutations (E434K, C764R, and L993F) in the voltage-sensitive sodium channel gene (vssc). In this study, the pyrethroid resistance state of the German cockroach in the Republic of Korea (ROK) was assessed by analyzing the frequencies of kdr mutations using one-step PCR with total RNA. The results revealed that among the 25 populations examined, 14 populations exhibited the L993F kdr mutation, while no other mutations were detected. Since other cockroach species are also commonly found in human dwellings in ROK, the vssc genes were cloned from four other species, including Blattella nipponica, Periplaneta americana, Periplaneta japonica, and Periplaneta fuliginosa. Based on the genomic DNA (gDNA) sequences obtained from the vssc cloning, primer sets were designed to amplify the vssc fragment spanning the L993F mutation for each species and used to monitor the development of pyrethroid resistance in cockroach populations in the ROK. The study will facilitate the implementation of a nationwide monitoring program to assess cockroach resistance and select suitable alternatives.

KHNP is conducting research to decommission Wolsong Unit 1 Calandria. Establishment of preparation and dismantlement processes, conceptual design of equipment and temporary radiation protection facilities, and waste management are being established. In particular, the ALARA plan is to be established by performing exposure dose evaluation for workers. This study aims to deal with the methodology of evaluating exposure dose based on the calandria dismantling process. The preparation process consists of bringing in and installing tooling and devices, and removing interference facilities to secure work space. The main source term for the preparation process is the calandria structure itself and crud of feeders. In the case of the dismantlement process, a structure with a shape that changes according to the process was modeled as a radiation source. It is intended to estimate the exposure dose by selecting the number of workers, time, and location required for each process in the radiation field evaluated according to the preparation and dismantlement process. In addition, it is also conducting an evaluation of the impact on dust generated by cutting operations and the human impact of C-14, H-3, which are specialized nuclides for heavy water reactors. KHNP is conducting an exposure dose evaluation based on a process based on the preparation and dismantlement process for decommissioning Calandria through computation code analysis. If additional worker protection measures are deemed necessary through dose evaluation according to this methodology, the process is improved to prepare for the dismantling of worker safety priorities.

Customers’ service experience is related to the perceived state in the process of interacting with the target at various channel touchpoints (Kaushal & Yadav, 2023). Furthermore, positive customers’ service experiences can increase the repurchase intention of buyers by strengthening the interconnection and cooperation between buyers and suppliers in business-to-business (B2B) firms and reinforcing corporate competitive positioning.

KHNP is carrying out international technical cooperation and joint research projects to decommission Wolsong unit 1 reactor. Construction data of the reactor structures, experience data on the pressure tube replacement projects, and the operation history were reviewed, and the amount of dismantled waste was calculated and waste was classified through activation analysis. By reviewing COG (CANDU owners Group) technical cooperation and experience in refurbishment projects, KHNP’s unique Wolsong unit 1 reactor decommissioning process was established, and basic design of a number of decommissioning equipment was carried out. Based on this, a study is being conducted to estimate the worker dose of dismantling workers. In order to evaluate the dose of external exposure of dismantling workers, detailed preparation and dismantling processes and radiation field evaluation of activated structures are required. The preparation process can be divided into dismantlement of existing facilities that interfere with the reactor dismantling work and construction of various facilities for the dismantlement process. Through process details, the work time, manpower, and location required for each process will be calculated. Radiation field evaluation takes into account changes in the shape of structures by process and calculates millions of areas by process, so integrated scripts are developed and utilized to integrate input text data. If the radiation field evaluation confirms that the radiation risk of workers is high, mutual feedback will be exchanged so that the process can be improved, such as the installation of temporary shields. The results of this study will be used as basic data for the final decommissioning plan for Wolsong unit 1. By reasonably estimating the dose of workers through computer analysis, safety will be the top priority when decommissioning.

Prevention of radiation hazards to workers and the environment in the event of decommissioning nuclear power plants is a top priority. To this end, it is essential to continuously perform radiation characterization before and during decommissioning. In operating nuclear power plants, various detectors are used depending on the purpose of measurement. Portable detectors used in power plants have excellent portability, but there is a limit to the use of a single measuring device alone to quantify radioactive contamination, nuclide analysis, and ensure representation of measurement results. In foreign countries, gamma-ray visualization detectors are being actively used for operating and decommissioning nuclear power plants. KHNP is also conducting research on the development of gamma-ray visualization detectors for multipurpose field measurement at decommissioning nuclear power plants. It aims to develop detectors capable of visualizing radioactive contamination, analyzing nuclides, estimating radioactivity, and estimating dose rates. To this end, we are developing related software according to the development process by purchasing sensors from H3D, which account for more than 75% of the US gamma-ray visualization detector market. In addition, field tests are planned in the order of Wolsong Unit 1 and Kori Unit 1 with Research reactor in Gongneung-dong in accordance with the progress of development. The detector will be optimized by analyzing the test results according to various gamma radiation field environments. The development detector will be used for various measurement purposes for Kori unit 1 and Wolsong

Air conditioning facilities in nuclear power plants use pre-filters, HEPA filters, activated carbon filters, and bag filters to remove radionuclides and other harmful substances in the atmosphere. Spent filters generate more than 100 drums per year per a nuclear power plant and are stored in temporary radioactive waste storage. Plasma torch melting technology is a method that can dramatically reduce volume by burning and melting combustible, non-flammable, and mixed wastes using plasma jet heat sources of 1,600°C or higher and arc Joule heat using electric energy, which is clean energy. KHNP CRI & KPS are developing and improving waste treatment technology using MW-class plasma torch melting facilities to stably treat and reduce the volume of radioactive waste. This study aims to develop an operation process to reduce the volume of bag filter waste generated from the air conditioning system of nuclear power plants using plasma torch melting technology, and to stably treat and dispose of it. It is expected to secure stability and reduce treatment costs of regularly generated filter waste treatment, and contribute to the export of radioactive waste treatment technology by upgrading plasma torch melting technology in the future.

Bacterial phytopathogen Pectobacterium causes soft rot disease in several vegetable crops globally, resulting in heavy agricultural losses at both the pre and postharvest stages. The present work was carried out to screen Kimchi cabbage genetic resources conserved at the National Agrobiodiversity Center, Rural Development Administration, Korea, for resistance against the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum KACC 21701 over a period of three years (from 2020 to 2022). Infection of the phytopathogen was carried out at four-leaf stage and for each accession, twenty-five plants per germplasm were infected with KACC 21701. Kimchi cabbage cultivars Wangmatbaechu, Seoulbaechu, and CR Kiyoshi were used as control. Seven-days post-infection, the Disease Index (DI) values were manually recorded from zero to four, zero matched perfectly heathy plants and four completely dead plants. The 682 accessions of Kimchi cabbage exhibited varying degrees of disease resistance to KACC 21701 and thirty accessions, exhibiting a DI≤2, were considered for replication studies. During the replication studies, four landrace germplasms (IT102883, IT120036, IT120044, and IT120048) and one cultivar (IT187919) were confirmed to be moderately susceptible to KACC 21701. Results of the preliminary screening as well as replication studies were documented for the all the 682 germplasms. Addition of such information to the passport data of stored germplasms might serve as potential bio-resource for future breeders and researchers to develop resistant varieties or study the mechanisms involved in resistance of plants to such phytopathogen.

Although probiotics have been shown to improve health when consumed, recent studies have reported that they can cause unwanted side effects due to bacterial-human interactions. Therefore, the importance of prebiotics that can form beneficial microbiome in the gut has been emphasized. This study isolated and identified bacteria capable of producing biopoymer as a candidate prebiotic from traditional fermented foods. The isolated and identified strain was named WCYSK01 (Wissella sp. strain YSK01). The composition of the medium for culturing this strain was prepared by dissolving 3 g K2HPO4, 0.2 g MgSO4, 0.05 g CaCl2, 0.1 g NaCl in 1 L of distilled water. The LMBP(low molecular weight biopoymers) produced when fermentation was performed with sucrose and maltose as substrates were mainly consisted of DP3 (degree of polymer; isomaltotriose), DP4 (isomaltotetraose), DP5 (isomaltopentaose), and DP6 (isomaltoheptaose). The optimization of LMBP (low molecular weight of biopolymer) production was performed using the response surface methodology. The fermentation process temperature range of 18 to 32oC, the fermentation medium pH in the range of 5.1 to 7.9. The yield of LMBP production by the strain was found to be significantly affected by q fermentation temperature and pH. The optimal fermentation conditions were found at the normal point, and the production yield was more than 75% at pH 7.5 and temperature of 23oC.

This study presents a rapid and quantitative radiochemical separation method for Nb isotopes in radioactive waste samples from the nuclear power plant with anion exchange resin after Fe coprecipitation. After radionuclides were leached from the radioactive waste samples with concentrated HCl and HNO3, the Nb isotopes were coprecipitated with Fe after filtering the leaching solution with 0.45 micron HA filter, while the Sr, Tc and Ni isotopes were in the solution. The Nb isotopes were separated in HCl medium with anion exchange resin. The purified Nb isotopes were measured using a low level liquid scintillation counter after installing quenching curve with standard Nb-94 isotopes. The separation method for Nb isotopes investigated in this study was applied to neutron dosimeter samples from the nuclear power plant after validating the Nb activity concentration with gamma spectrometry system.

Plasma Arc Melter (MSO) system has been developed for the treatment and the stabilization of various kinds of hazardous and radioactive waste into the readily disposable solidification products. Molten salt oxidation system has been developed for the for the treatment of halogen- and sulfurbearing hazardous and radioactive waste without emissions of PCDD/Fs and acid gases. However, PAM system has showed some difficulty in the off-gas treatment system due to the volatilization of radionuclides and toxic metals at extremely high-temperature plasma arc melter and the emissions of acid gases. MSO system has also showed the difficulty in the treatment of spent molten salt into the disposable waste form. Present study discussed the results of organics destruction performance tests for the PAM-MSO combination system, which is proposed and developed to compensate the drawbacks of each system. The worst-case condition tests for the organics destruction were conducted at lowest temperatures and the worst-case condition tests for the retention of metals and radionuclides were conducted at highested temperatures under the range of normal operating condition. For the worst-case organic destruction test, C6H5Cl was selected as a POHCs (Principal Organic Hazardous Constituents) because of its high incinerability ranking and the property of generation of chlorine gases and PCDD/Fs when incompletely destroyed. Simulated concrete waste spiked with 1 L of C6H5Cl was treated and the emissions of 17 kinds of PCDD/Fs and other hazardous gases such as CO, THCs, NOx, SO2 and HCl/Cl2 were measured. For the worst-case condition tests for the retention of metals and radionuclides, Pb and Cs were selected because of its high volatility characteristics. The emissions of PCDD/Fs was extremely lowered than the emission limit and those of other hazardous constituents were below their emission limit. The results of performance tests on the organics destruction suggested that tested PAM-MSO combination system could readily treat PCBs-bearing spent insulation liquid, spent ion-exchange resins used for the treatment of spent decontamination liquid in the decommission process and the concreted debris bearing hazardous organic coating materials. The decontamination factor of Cs and Co were 1.4×105, 1.4×105, respectively. The emisison of Pb was 0.562 ppm. These results suggested that tested PAM-MSO system treated low-level radioactive and pb-bearing mixed waste.

Present study investigated the waste form integrity of melted products generated from PAM-MSO system, which is proposed and developed to compensate the drawbacks of each system. The disposal suitability of the melting solidification products generated from the plasma arc melting treatment of pulverized cement debris spiked by Pb, Cd and Cs, as indicators of typical hazardous metals and radionuclides existed in the low-level mixed waste in the KHNPPs. The final waste form obtained by the test was evaluated for suitability for disposal. The compressive strength was 261.10 MPa, showing much higher values when compared to other waste form products. The compressive strength of both the sample after irradiation with 107 Gy radiation and that after long-term submersion test (90 days) satisfied the disposal criteria. As a result of the leaching test conducted according to the ANS 16.1 test method, it was confirmed that the leaching index satisfies the disposal criteria.