Epithelial-mesenchymal transition(EMT) plays a pivotal role in the convers ion of earl y s tage tumors into invasive malignancies‘ and has been shown to be regulated hy the transcri ptional factor. Snail. Recent ly‘ actlvatlon of the phosphatidylinositol 3' kinase (PI3K)/따<:T axis is emerging as a centra l feature of EMT‘ However. it is unclear whether the phosphorylation of AKT regulate the expl'ession of s nail in ora l cancer cell underwent EMT. T。 investigate a role of p-AKT in EMT, we assessed the effects of inhibi ting p-AK1' activity in oral squamous can cer cells(KOSCC-25B) using PIAs, structurally modified phosphatidyli nositol ether lipid analogues(P1As) . PIAs de creased phosphorylation of c-Jun N-terrninal Kinase(JNK) and increased phosphorylation of glycogen synthase kinase 3beta(GSK-3beta). Inhibition of p-AKT ir빼ce d down regulation of Snail and Twist. but Sip1 regulated independent of p-AKT inhibition. Also inhibi tion of p-AKT dec reased cell migration and invas ion. Therefore our results implicate that p-AKT may contribute to the translocalization of sna il in the EMT associated with canceJ cell rnigration and invasion

Although a number of molecules have been implicated in the metastasis of oral squamous cell carcinoma (OSCC) , the precise molecular mechanisms that deterrnine the direction of rnigration and invasiveness of OSCC cells into the lymph nodes remain unclear, Chemokines are a superfarnily of small structurally related heparin- binding proteins‘ which have been identified as attractants that control the rnigration of leukocytes‘ especia lly during imrnune and inflammatory reactlOns Moreover, recent studies have demonstrated that several types 。f cancer express chemokine receptor‘s and use chemokines to metastasize to the target organ, However, there h ave been few reports on biological behaviors by downregulation 0 1' CXCR-4 in ora l cancel‘ cells We tried to screen several OSCC cell lines in order to obtain a suitable cell line model which had the cha ract eris tic of the constitutive ly expressed state of CXCR• 4 Of the several OSCC cell lines, only KOSCC-25B showed the high expression of CXCR-4 in both RT-PCR and Western blot analysis, siRNA-CXCR-4 infected subclones of KOSCC-25B(Si, 3‘ Si 1이 showed downregulation of CXCR-4 expression as expected‘ At serum-free co ndi tion‘ Si.3 s ubclone s ignif icantly decreased cell proliferations at 24 h and 48h and Si, lO subclone significant ly dec reased cell proliferations at 24 h Si ,3 clone dec reased to 67 ,4% and Si,lO clone to 65 ,5% in comparison to vector infected cells These data suggest that the downregulation of CXCR-4 expression could induce anti-rnigratory and ant i- rni g ratory effect

This study was carried out to investigate the chemical 　composition of peanut mutants induced by gamma radiation (Co-60) at 300 ㏉ in seeds of the cultivar Shinnamkwang. The protein contents of twenty-eight peanut mutants ranged from 23.3% to 31.7% and were increased by from 0.5% to 8.4% in fifteen mutants lines from the 26.8% of the original variety, cv. Shinnamkwang. Lipid contents of in mutants ranged from 43.2% to 53.5%, an increase of 0.2% to 5.7% from the 47.8% of the original variety. The range of unsaturated oleic acid in 28 mutants was from 38.9% to 56.9% an increase of from 1.3% to 14.0% from the 50.6% in the original variety. Linoleic acid, the highest unsaturated fatty acid, constituted 32.6% meanly of mutants, it was 17.4% lesser than oleic acid, ranging from 25.9% to 42.0%. Palmitic acid (16:0) contents ranged from 8.6% to 11.1%, and the mutant line-9 had the highest content. The ratio of oleic to linoleic acid was 0.9 to 2.2. A negative coefficient (r=-0.98**) was obtained between oleic and linoleic acid, but for other fatty acids, no significant relation was observed. Similarly, a negative coefficient of r=-0.68**was observed between saturated and unsaturated acids. The sucrose compositions of mutants ranged from 2.6% to 6.2%.

Wild peanut species have proven to be an important germplasm resource. The objective of this study was to characterize interspecific crossability among 3 cultivars and 5 wild species of the peanut. Hybrids were acquired from crosses among three cultivars and five wild diploid species, respectively. A. sylvestirs A. Chev. evidenced the highest degree of crossability among the three cultivars. In the Valencia type, the combination of A. hypogaea L. cv. IT187846 and A. sylvestirs A. Chev. formed 32.7% pegs. Pods were developed in 98.8%, and seeds were produced at a rate of 49.4%, a higher percentage than was observed with the Virginia or Spanish types. Peg formation from pollinated flowers was increased by 18.4∼62.1% via the application of GA3, as opposed to the 26.3∼34.7% control value. The combination of A. hypogaea L. cv. Daekwang and A. batizocoi Krapov. & W. C. Gregory resulted in 93.9% peg formation. Seeds set in 24.2∼26.9% from the pegs, representing a 4.2∼16.3% increase over the 10.8∼24.3% control values. As the result of GA3 treatment of flowers after pollination, the peg emergence ratio increased significantly, but seed formation increased only by a small amount. The pods of the interspecific hybrid were smaller than those of A. hypogaea L. cv. Daekwang, and a portion of the pod had immature seeds or abnormal seeds. Via PCR-RAPD method, we were able to determine that the exotic DNA had been integrated from A. batizocoi Krapov. & W. C. Gregory to A. hypogaea L. cv. Daekwang.

The genus Arachis L. is distributed widely throughout South America. This genus encompasses 80 described species, and 31 species belong to section Arachis, which is divided into diploid and tetraploid species. The objective of this study was to determine the morphological characteristics and genetic relationships among A. hypogaea L. cv. Daekwang and thirteen germplasms, using RAPD techniques. Peanut plants were cultivated in pots (1.2×0.8 m2) with clay loam in a glass-covered greenhouse and genetic relationship was identified by DNA polymorphisms using PCR. Thirteen germplasms bloomed between June 18th and June 25th, the majority of which bloomed 3∼10 days later than Daekwang (June 15th). The leaf size of A. batizocoi Krapov. & W. C. Gregory was similar to that of Daekwang, three germplasms, A. monticola Krapov. & Rig., A. paraguariensis Chodat & Hassler, and A. dardani Krapov. & W. C. Gregory, were bigger than Daekwang, but the others were smaller. Twelve germplasms evidenced peduncle longer than those of A. hypogaea L. cv. Daekwang. All of the seeds of the germplasms were smaller than those of Daekwang (76.2 g), and the weight of 100 mature seeds ranged from 6.0∼36.1 g in sections Arachis, Erectoides, Procumbentes, and Heteranthaes. The genetic relationship distance was determined among Daekwang, A. sylvestris, A. monticola, and A. batizocoi.

A new vegetable peanut variety “Bakjung(Arachis hypogaea L.)” was developed from the cross between Shindaekwang and Suwon94 at the Honam Agricultural Research Institute and Yongnam Agricultural Research Institute, National Institute of Crop Science in 200

A new peanut variety “Kokwang(Arachis hypogaea L.)” was developed from the cross between Florigiant and Tifapan at the Honam Agricultural Research Institute in 2003. This variety has shinpung type and erect growth habit with midium size obovate dark green

A new peanut variety “Daemyung(Arachis hypogaea L.)” was developed from the cross between SP8703-2-2 and SP8708-4-1 at the Honam Agricultural Research Institute, National Institute of Crop Sciencee in 2003. This variety belongs to shinpung type with erect

Peanut (Arachis hypogaea L.) mutants were induced by the gamma ray with 300 Gy of Co-60 irradiation using the seed of cultivar Shinnamkwang. BTM3-9 and BTM5-19 lines were selected in M₃ generation and then segregated. In M₈ generatio

Peanut (Arachis hypogaea L.) mutants were induced by the gamma ray with 300 Gy of Co-60 irradiation using the seed of cultivar Shinnamkwang. BTM3-9 and BTM5-19 lines were selected in M3 generation and then segregated. In M8 generation, the yield potential

A new peanut variety “Dakwang (Arachis hypogaea L.)” was developed from the cross between Suwon80 and ICG11 at the National Honam Agricultural Experiment Station(NHAES) in 2001. This variety has Shinpung type and erect growth habit with large size obovate

“ Namahn” (Sesamum indicum L.) was developed at the National Honam Agricultural Experiment Station (NHAES) in 2001. It was derived from the cross between “Suwon87 ” of white seed coat color and short stem, and “Nongki S-2” of phytophthora blight tolerance