낙동강하굿둑의 건설로 원활한 용수 공급이 이루어졌으나, 하구 수생태계의 종적 연결성을 단절시켰다. 이에 낙동강 하굿둑 개방에 대한 사회적 요구가 지속적으로 제기되어 왔으며, 2017년부터 낙동강하구역 기수생태계 복원을 위한 노력이 본격화되었다. 낙동강 하구에는 다양한 어종이 서식해 왔으며, 이들의 서식·이동 특성은 상이하므로 이를 고려한 수문운영은 기수생태계 복원에 필수적이다. 이에 본 연구에서는 낙동강하구에 서식·이동하는 어류를 모니터링을 통해 확인하였으며, 방류 시 수문운영 형태별, 개도별 평균 유속을 산출하여, 어종에 따른 원활한 소상 가능성을 분석하였다. 또한 대상어종을 선정하고, 주 분포 수심에 따른 수문운영 형태를 제시하는 등 낙동강 하구역의 기수생태계 복원을 적극적으로 수행하기 위한 수문운영 방안을 제시하였다.

본 연구는 낙동강하굿둑 개방으로 인한 하구 인근의 식생 변화를 모니터링하기 위해서 현재 식생에 대해서 조사하고, 하구수변식생평가지수를 통해서 식생의 건강성을 평가하였다. 장기모니터링의 첫 번째 조사로 낙동강하구 인근의 6개 구역에 대해서 2021년 7월과 10월 2차례 수행하였으며, 식생모니터링 및 수생식물, 수변식물, 육상식물의 종조성, 분포밀도 조사를 진행하였다. 조사구역 내에서 확인된 관속식물은 82과 192속 230종 1아종 28변종 3품종 262분류군으 로 파악되었다. KREVI를 통한 식생건강성 평가 결과는 1, 6번 구역이 두 차례 조사에서 모두 ‘매우 좋음’ 등급이었고, 2, 4번 구역은 ‘매우 좋음’에서 ‘좋음’으로 2차 조사결과가 낮게 산정되었으며, 3, 5번 구역은 1차 조사에 비해 2차 조사결과가 1등급 상향되었다. 조사구역 대부분의 건강성 등급이 대체로 높게 평가되었다. 기수역 조성을 위한 수문개 방 후 잠재자연식생의 대부분은 갈대군락으로 이루어질 것으로 예상되며, 수역과 인접한 버드나무군락의 경우 다소 면적이 좁아질 수 있으나 군락 형태를 유지하고, 염분농도가 매우 낮은 내륙지역 담수역의 경우 갈대가 대부분을 차지하며, 일부 물억새, 부들 등의 군락이 분포할 것으로 사료된다. 장기모니터링을 통해 이런 예상되는 변화를 관찰하 며, 건강한 기수역이 조성될 수 있도록 하굿둑 개방 운영방안 등 대책이 수립되어야 할 것으로 판단된다.

Alpha-linolenic acid (ALA) is one of n-3 polyunsaturated fatty acids and found mainly in the chloroplasts. Many studies have been reported that intracellular reactive oxygen species (ROS) in mammalian oocytes were reduced by supplementation of ALA in in vitro maturation (IVM) medium. Based on these reports, we expected that ALA acts as an antioxidant during IVM of porcine oocytes. Therefore, the objective of this study was to investigate the antioxidant effect of ALA supplementation during IVM in porcine oocytes. The cumulus-oocyte complexes (COCs) were incubated in IVM medium containing 200 μM H2O2 or H2O2 with 50 μM ALA for 44 h. Nuclear maturation stage of oocytes was evaluated using aceto-orcein method. For measurement of oxidative stress state, intracellular ROS and glutathione (GSH) levels were measured using carboxy-DCFDA and cell tracker red, respectively. In results, oocytes in metaphase-II (MII) stage development was significantly reduced in H2O2 group compared to non-treated control group (61.84±1.42% and 80.00%, respectively; p<0.05) and it was slightly recovered by treatment of ALA (69.76±1.67%; p<0.05). The intracellular GSH levels was decreased in H2O2 groups compared with control groups, but it was enhanced by ALA treatment (p<0.05). On the contrary, H2O2 treatment increased intracellular ROS level in oocytes and H2O2-induced ROS was decreased by treatment of ALA (p<0.05). Our findings suggested that ALA treatment under oxidative stress condition improve oocyte maturation via elevated GSH and reduced ROS levels in oocytes. Therefore, these results suggest that ALA have an antioxidative ability and it could be used as antioxidant in in vitro production system of porcine embryo.

The oocyte undergoes various events during In vitro maturation (IVM) and subsequence development. One of the events is production of reactive oxygen species (ROS) that is a normal process of cell metabolism. But imbalances between ROS production and antioxidant systems induce oxidative stress that negatively affect to mammalian reproductive process. In vitro environments, In vitro matured oocytes have many problems, such as excessive production of ROS and imperfect cytoplasmic maturation. Therefore, In vitro matured oocytes still have lower maturation rates and developmental competence than in vivo matured oocytes. In order to improve the IVM and In vitro culture (IVC) system, antioxidants, vitamins were added to the IVM, IVC medium. Antioxidant supplementation was effective in controlling the production of ROS and it continues to be explored as a potential strategy to overcome mammalian reproductive disorders. Based on these studies, we expect that the use of antioxidants in porcine oocytes could improved maturation and development rates.

The aim of this study was to investigate change of plasminogen activators (PAs) and their inhibitors (PAIs) mRNA and protein expression level by heat stress in porcine endometrial cells. The endometrial epithelial cells were isolated from endometrial epithelium in porcine uterus and cultured in different temperature conditions (38.5 and 41.5℃) for 24 h. Expression of urokinase-type PA (uPA), tissue-type PA (tPA), PA inhibitor-1 (PAI-1) and -2 (PAI-2) mRNA in epithelial cells were analyzed using reverse transcription-PCR and protein levels were measured by immunofluorescence. In result, mRNA expression of uPA, tPA, PAI-1 and PAI-2 were decreased in 41.5℃ than 38.5℃ culture condition, however, significant differences were no detected. uPA, tPA and PAI-2 protein were mainly expressed in nucleus, whereas PAI-1 was distributed in cytoplasm and nucleus. uPA and tPA protein levels were increased by heat stress treatment and significant difference was only detected in tPA level (p<0.05). In contrast, two types of PAIs protein level were decreased in 41.5℃ cultured group compared with 38.5℃ group. In present study, tPA protein expression was upregulated by heat stress in porcine endometrial cells. This result suggest that change of tPA by heat stress may be related to blood flow into uterus and intrauterine microenvironments, and could directly and indirectly influence to reproductive performance in pigs.

The objective of this study was to investigate to influence of glutathione (GSH) on development and antioxidant enzyme activity in tetraploid porcine embryos. Tetraploid embryos were produced using parthenogenetic 2-cell embryo by electrofusion method. Tetraploid embryo development was observed every 24 hours and intracellular antioxidant enzyme activity was measured at 120 hours after electrofusion. The 4-cell to 16-cell stage tetraploid embryos was increased in 100 and 500 μM GSH-treated groups compared control group at 48 hours (P < 0.05) but cleavage rates were not significantly different among the GSH treatment groups at 48, 72, 96, and 120 hours. Blastocyst formation was significantly increased by 300 and 500 μM GSH at 120 hours in tetraploid embryos (P < 0.05). But blastocyst cell number were not significantly different among the GSH treatment groups (16.4 ± 0.8, 16.8 ± 2.6, 18.5 ± 2.8 and 17.5 ± 1.8). The intracellular antioxidant enzyme level was increased in 500 μM GSH compared to 0 and 100 μM GSH (P < 0.05). We suggest that GSH may be improve development of tetraploid embryo in pigs.

The aim of this study was to evaluate effect of α-linolenic acid (ALA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved in 20% egg yolk freezing extender containing ALA (0, 3, 5, and 10 ng/mL) with 0.05% ethanol. The frozen-boar spermatozoa were thawed at 37.5°C for 45 sec in water-bath. The spermatozoa samples were evaluated the plasma membrane integrity, acrosome reaction, and mitochondrial integrity using flow cytometry. In results, population of live sperm with intact plasma membrane was significantly higher in control and 3 ng/mL ALA treatment group than ethanol group (p<0.05). In contract, dying sperms were higher in ethanol group than 3 ng/mL ALA treatment (p<0.05). Acrosomal membrane damage in all sperm population was reduced in 3 ng/mL ALA groups compared with ethanol treatment (p<0.05). However, acrosome damage in live sperm population was no significant difference among the all treatment groups. Mitochondrial integrity was not influenced by ALA treatments in both of live and all sperm population. In conclusion, this results show that supplement of ALA during the cryopreservation process could reduce the membrane damages including plasma and acrosomal membrane, whereas ALA did not influence to mitochondria in boar spermatozoa. Therefore, these results suggest that ALA can protect against the membrane damage derived cryo-stress, and cryopreservation efficiency of boar semen would be improved by use of ALA.

As a one of unsaturated fatty acid, polyunsaturated fatty acids (PUFAs) have multiple actions: as precursor of prostaglandins (PGs), steroid hormone synthesis and energy production in animal reproduction. PUFAs, which include omega-3 (n-3) and omega-6 (n-6), are derived from the diet and changed by diet, species, breed and season. The plasma membrane of spermatozoa in mammals contain various PUFAs. These composition of PUFAs regulate the membrane fluidity and cause lipid peroxidation via generation of reactive oxygen species (ROS). Induced lipid peroxidation by ROS decreased viability and motility of spermatozoa, and it is reduced by addition of antioxidant and low concentration of PUFAs. Because oocytes of animal have a high lipid components, process of oocyte maturation and embryo development are influenced by PUFAs. In in vitro study, oocyte maturation, embryo development, intracellular cAMP and MAPK activity were increased by treatment of n-3 α-linolenic acid (ALA) during maturation, whereas n-6 linoleic acid (LA) negatively influenced. Also, inhibition of fatty acid metabolism in oocyte influenced blastocyst formation of cattle. PGs are synthesized from PUFAs and various PUFAs influence PGs via regulation of PG-endoperoxide synthase (PTGS). Steroid hormone synthesis from cholesterol is regulated by expression of steroid acute regulator (StAR) protein and mRNA. Exogenous n-3 and n-6 PUFAs altered sex hormone in animal through stimulate or inhibit StAR activity. Because PUFAs altered PG and steroid hormone synthesis, follicular development was influenced by PUFAs. This effect of unsaturated fatty acid could provide information for improvement of reproductive ability in animals.

본 연구에서는 앞선 연구를 통해 도출된 해양사고 결과와 안전관리 결과 등의 평가항목을 이용하여, 해운선사의 안전관리 수준을 평가할 수 있는 해운선사 안전관리 평가지표를 개발하였다. 또한 평가항목을 구성하는 평가요소의 가중치를 산정하기 위하여 해양사고 조사 관리 업무를 담당하는 공무원, 검사원 등으로 이루어진 전문가그룹을 구성하고, 델파이 기법을 이용해 평가항목을 구성하는 평가요소의 가중치를 산정하였다. 본 연구에서 개발된 안전관리 평가지표의 유효성을 검증하기 위해 외항 해운선사 119개 업체(국적선박 916척)를 보유선박수와 총톤수를 기준으로 小, 中, 大 세 그룹으로 그룹화 하고, 안전관리 수준을 평가하여 안전관리가 우수한 19개 해운선사를 식별하였다. 小그룹(Group 1)과 中그룹(Group 2)은 각각 8개 업체, 大그룹(Group 3)은 3개 업체가 평가지표 1.0 미만으로 양호한 안전관리 수준을 나타냈다. 이들 업체는 최근 3년간 해양사고 발생건수가 없었고, 항만국통제 점검과 안전관리체제 심사에 있어서도 출항정지, 중부적합사항이 없거나 현저히 낮은 수준을 보였다.

국가 간 국제무역 확대로 인한 물동량 증가, 국민의 삶의 질 향상에 따른 해양레저 활동의 증가 등 해상교통 환경은 빠르게 변하고 있다. 또한 선원의 고령화, 외국인 선원 증가 등 선박운항 환경도 변화하고 있다. 이러한 해상교통 및 선박운항 환경변화에 따라 시장에서는 해운선사의 수준 높은 안전관리 능력을 요구하고 있다. 본 논문에서는 해운선사의 해양사고, 항만국통제, 안전관리체제 등 개별 제도에 따른 안전관리 결과를 종합적으로 평가 및 관리할 수 있는 제도 도입을 위하여 국내외 유사사례를 조사 분석하고 모방전략을 적용했다. 모방전략을 이용한 연구결과에 있어서 모방산업과 제도는 건설업체 환산재해율 산정제도, 교통안전 우수사업자 지정제도를 선정했다. 두 제도를 분석하여 모방대상으로 근거법률, 평가지표, 인센티브를 추출하고 해운선사 안전관리 수준 평가제도를 위한 근거법 도입, 해양사고율, 항만국통제 점검률, 안전관리체제 심사율로 구성된 평가지표와 안전점검의 면제, 수수료 감면 및 심사시간 단축 등의 인센티브 방안을 제안하였다. 또한 제도의 신속한 도입과 지속적인 시행을 위한 모방시점과 방법을 단계별로 제시하여 제도의 지속발전이 가능하도록 하였다.

This study was performed to investigate health-related behaviors and dietary attitudes among workers, using questionnaires with 338 workers in October 2005 who were employed by 5 industrial companies at the Gumi industrial complex. The result is summarized as follows. The subjects' interest in health was shown to be slightly higher than usual. An increase in their interest was observed with advancing age (p<0.05) and the level of interest was higher in females than males(p<0.05). Regarding a understanding of their health status, 57.4% of the total subjects considered their health status to be poor although there was no any known disease, while only 29.6% considered to be good. The subjects' dietary attitudes showed that the average of the each items was 2.88±0.82, and the average of the total items was 58.7±0.00. The average of their diet satisfaction was shown to be 2.88±0.82, and There was a significant difference in their diet satisfaction between residential building types(p<0.01) and work types(p<0.01). 66.8% of the total subjects answered that the main purpose of their meals was to fill an empty stomach. Their health-related behaviors, dietary attitudes and diet satisfaction showed that daily exercise, suitable diet and intake of health supplements were closely correlated with their dietary attitudes. It was also shown that there were the close correlations between the suitable diet and their dietary attitudes and diet satisfaction, between their refraining from drinking coffee and sufficient rest, between their refraining from drinking alcohol and sufficient rest, and between their feeling comfortable and drinking water as much as possible, respectively.

항로표지는 선박에 대하여 등광, 형상, 색채, 음향, 전파 등을 수단으로 운항 선박에게 위치, 방향 및 장애물의 위치 등을 알려주는 항행보조시설 일체를 의미하며, 주야간 선박항행의 안전을 위한 기본 수단이다. 예인선은 안전을 위해 종류별 최소 설비요건을 마련하여 사고를 대비하고 있지만, 예인삭의 길이, 조류의 영향, 상대적으로 중량이 큰 부선의 타력으로 인한 영향 등으로 예인선과 선회권이 달라, 변침 시 부선이 예인선을 따라가지 못하여 등부표와 추돌하는 사고가 발생하기도 한다. 본 연구의 목적은 두 선박의 물리적 관계를 분석하여 예인선 열을 식별하고 등부표와의 위험을 항로표지 관리운영 시스템을 통해 전파하는 기초 시스템을 제시하는데 있다. 연구를 통해 예부선의 사전 식별 가능성을 확인하였고, 위험 전파를 위한 메시지를 설계하고 모의 시스템을 통한 위험 전파 및 전자해도 상에 위험을 표시하였다.

The maritime industry has emerged as a new growth engine. The municipalities that own the port are trying to add value through the maritime industry. Overseas port cities are also creating maritime industrial clusters to generate profits and strengthen competitiveness. Therefore, it is very important to understand the comparative status of the domestic maritime industry by region. Based on this analysis, it is possible to establish maritime industrial clusters and strategies for integration. This study analyzed the structure of the maritime industry located in Jeollanamdo province, the southwestern part of Korea. Through the analysis of existing literature, the maritime industry was reclassified into 5 major categories, 21 subcategories, and 84 subcategories. Based on the reclassified maritime industry, the analysis of the Jeollanamdo province maritime industry was based on applying the location quotient and the shift-share analysis. As a result of analyzing the geographical location of Jeollanamdo province, other industries showed the highest value of 2.790, followed by fisheries (2.227), shipbuilding industry (1.164) and marine tourism industry (0.554). The growth effect of the maritime industry in Jeollanamdo province was 35,323 people, and net growth effect excluding national growth effect was 11,945 people. In particular, the net growth effect of the shipbuilding industry was the highest at 11,320, followed by shipping logistics (6,371) and marine tourism (1,529). On the other hand, there was no net growth effect in fisheries. The results of this study can be used as basic data for the construction of the maritime industrial cluster for Jellanamdo province in the future.

본 연구의 항만보안 향상을 위해 중요한 평가요인들을 발굴하고 요인들 간 상대적 중요도를 분석하는데 있다. 이를 위해 문헌조사 및 항만보안 업무 종사자들로 구성된 전문가 그룹의 브레인스토밍(Brainstorming)을 통해 평가요인을 선정하여, 4개의 대분류 요인과 12개의 중분류 요인으로 분류하였다. 계층적으로 분류된 평가요인을 전국의 항만보안 관련 업무를 수행하고 있는 공무원, 항만공사와 항만보안공사 종사자, 항만보안관련 교육기관 종사자들을 대상으로 설문조사를 실시하고, 계층분석법(AHP)을 이용하여 중요도를 산출하였다. 또한 그룹 간 중요도 인식 차이를 알아보기 위하여 통계 분석을 실시하였다. 평가요인 중요도 분석 결과, 보안 운영시스템 강화 요인이 가장 높았고 하드웨어 개선, 보안인력 증원의 순으로 나타났다. 또한 대분류의 정책적 지원 및 시설 지원, 인력ㆍ예산 지원에서 그룹 간 인식 차이를 확인할 수 있었다. 분석 결과를 통해 항만 내 통합모니터링시스템 구축을 통한 보안 운영체제의 개선이 우선적으로 요구되며, 그룹 간 인식 차이를 이해 하고 이를 바탕으로 유기적인 협조체제의 구축이 필요함을 알 수 있었다. 이 연구결과는 후속연구로 수행될 국내 항만보안 효율성 측정을 위한 평가요인으로 활용될 수 있을 것으로 기대된다.

The aim of this study was to determine the effect of additional alpha-linolenic acid (ALA) supplementation during in vitro maturation (IVM) and culture (IVC) on nucleic maturation and embryo development of pigs. Cumulus-oocyte complexes (COCs) were incubated in IVM medium containing different concentration of ALA (25, 50 and 100 μM) for 44 h. After in vitro maturation, nuclear maturation of oocytes were evaluated by aceto-orcein stain. Mature oocytes with 50 μM ALA were fertilized and cultured in IVC medium with ALA (25, 50 and 100 μM) during early-embryogenesis (48 hours after fertilization). Then, embryos were cultured with 25 μM ALA during early embryogenesis and/or late embryogenesis (120 hours after early-embryogenesis). In results, oocyte maturation were significantly increased by 50 μM ALA treatment groups compared with control groups (p<0.05). Treatment of 25 μM ALA during early-embryogenesis enhanced cleavage rate of embryo compared with other groups (p<0.05), whereas formation and total cell number of blastocyst had no significant difference. Similarly, cleavage rate of embryos were increased by 25 μM ALA supplement during early- or late-embryogenesis than ALA treatment both stage of embryogenesis (p<0.05), but did not influence to blastocyst formation. Interestingly, total cell number of blastocyst were enhanced in ALA treatment group during early-embryogenesis. These findings indicated that ALA supplement enhance the nuclear maturation of oocyte and embryo development, however, excessive ALA could negatively influence. Therefore, we suggest that ALA is used for improvement of in vitro production of mammalian embryo and further study regarding with functional mechanism of ALA is needed.