본 연구는 성주지역에 적합한 참외재배용 내재해형온 실을 개발하기 위하여 참외 재배온실의 구조현황 조사, 최근 기상자료가 반영된 설계하중 산정, 온실모델 설정 및 구조안전성 분석을 수행하였으며 결과를 요약하면 다음과 같다. 성주지역 참외온실의 구조적 특성을 파악하기 위하여 현장 조사한 결과, 온실의 지붕형상은 아치형이 가장 많았으며, 최근에 시공한 온실일수록 복숭아형이 많았으며 폭이 넓어지고 동고도 높아지는 추세였다. 그리고 성주지역에 가장 인접한 구미기상대의 기상자료를 이용하여 재현기간에 따른 설계풍속 및 설계적설심을 산정할 수 있는 계산식을 유도하였으며, 내재해형 온실 규격을 만족하는 재현기간 30년의 적설심은 23.7cm, 풍 속은 33.8m·s−1로 계산되었다. 이러한 성주지역의 내재해형 설계기준을 만족하는 참외재배용 단동온실 4종, 2연 동온실 및 연결온실을 각각 1종씩 총 6종의 모델을 개발하였다.

The Riptortus (stinkbug) has a specialized symbiotic organ, M4 midgut, to harboring symbiont Burkholderia. M4 midgut is located in abdomen and surrounded with insect hemolymph. Recently our group demonstrated that symbiotic Burkholderia showed different physiology after adapting in M4 gut compare with in vitro cultured Burkholderia. And population of symbiotic Burkholderia in the M4 midgut is regulated by special organ. However, the molecular mechanism to prevent spreading and migrating symbiont bacteria to other host tissues from symbiotic organ is not clear. Therefore, we assumed that symbiont Burkholderia are susceptible to host humoral immunity after established infection in M4 midgut to prevent spreading and migrating into the other host tissues through Riptortus hemolymph. To prove this assuming, we tested the susceptibility and survival rate of symbiont Burkholderia in hemolymph of Riptortus in vitro and in vivo. We also examined the susceptibility of symbiont Burkholderia using purified antimicrobial peptides (AMP), pyrrhocoricin-like, thanatin-like and defensin-like AMPs. Finally, we tested inducing ability for AMPs by systemic infection of symbiotic Burkholderia. Gene expression of purified AMPs was not different after systemic infection of both symbiont and in vitro cultured Burkholderia. Surprisingly, in vitro cultured Burkholderia resisted on bacteria injected hemolymph and purified AMPs but symbiont Burkholderia were highly susceptible in bacteria injected hemolymph and purified AMP. These results suggest that symbiont Burkholderia can't survive in the hemolymph after escaping symbiotic organ. Moreover, humoral immunity of host Riptortus is important to prevent spreading and migrating symbiont Burkholderia into the other host tissue or organ from symbiotic organ.

The resistance levels to carbamate (CB) and organophosphate (OP) insecticides were determined by topical application in 14 field strains of Nilaparvata lugens. The resistance levels to CB and OP were 1.3~47.5-fold and 1.4~14.4-fold higher than a susceptible strain, respectively. A quantitative sequencing (QS) protocol was established to determine the allele frequencies of four acetylcholinesterase point mutations putatively associated with CB and OP resistance. The allele frequencies of the four mutations (G119A, F/Y330S, F331H and I332L) in field strains ranged from ca. 0.0~51.7%, 0.0~88.9%, 2.5~47.7%, 5.1~56.0% and 6.7~57.3%, respectively. The F331H and I332L were tightly linked each other, suggesting these mutations may occur simultaneously. In the correlation analysis, G119A was not well correlated with actual resistance levels (r2= < 0.232), whereas F331H and I332L showed a better correlation with the resistance levels of benzofuranyl methylcarbamates (r2= 0.595). This finding indicates that F331H and I332L mutation frequencies may be used as molecular markers for detecting carbamate resistance in N. lugens. A QS protocol detecting the F331H and I332L mutation frequencies would be employed as a supportive tool for rapid monitoring of CB insecticide resistance levels in N. lugens

Background : Miscanthus sinensis is a C4 crop with high photosynthetic efficiency and is known as a representative cellulose-based bio-energy crop originating in East Asia such as Korea, Japan, and China. It also has a high resistance to environmental conditions such as low temperature and drying and pests. However, many studies on the antioxidant activity of the M. sinensis have not been conducted. Therefore, this study was conducted to measure total phenol and total flavonoid contents of the M. sinensis accessions collected from some countries and to select the M. sinensis accessions with rich total phenol and total flavonoid contents. The results of this study can be used as basic data for future breeding. Methods and Results : This study carried out the total phenol and total flavonoid contents analysis of M. sinensis collected from different country to preserve genetic resources. Total 205 accessions of M. sinensis were collected from the USA, China, Korea, Japan, Thailand and Zambia. Their leaves were extracted with 100% methanol and concentrated at 45℃. To determine total phenol and total flavonoid contents, we measured the total phenolic content and total flavonoid content. The highest total phenolic conten t(81.04 ± 0.02 ㎎·GAE/g) showed in the accession (PMS-178) from China. The highest total flavonoid content (32.66 ± 0.01 ㎎·QE/g) showed in the accession (KMS104) from Korea. On the other hand the lowest total phenolic content (1.11 ± 0.00 ㎎·GAE/g) and the total flavonoid content (0.81 ± 0.00 ㎎·QE/g) were obtained from the accession (M. sinensis ‘Super Stripe’) from USA. Conclusion : In this study, we analysed the total phenolic and total flavonoid contents by country of origin.

Background : Oplopanax elatus, comnonly called Asian devil’s club, is distributed in the northern high mountains of the Korean peninsula, northeastern China, and southeastern Russia. Its root is traditionally used to treat asthma, depressive states, chronic fatigue syndrome, and hypertension, neurasthenia, hypopiesis, schizophrenia, cardiovascular problems, diabetes mellitus, impotence, emaciation, hypotension cough, rheumatism, arthritis, gastrointestinal disorders, and wounds. Plant cell and tissue culture techniques for the mass production of adventitious roots in suspension culture allows a high proliferation rate, stable accumulation rate, and production of valuable compounds. Methods and Results : The plants were cultured on 1/4 MS medium with 1% sucrose and 1% plant agar for 3 month and they transferred to pot. The pots were contained of soil, soil + pelite (1 : 1), and soil + sand (1 : 1), respectively. The plants were measured plant length, leaf length, and leaf number. The number of leaves have represented as similar result of 7.44 ± 1.34 ㎝ in soil and 7.56 ± 1.26 ㎝ on soil + pelite. The 3.22 ± 0.53 ㎝ of plant length and the 6.57 ± 0.51 ㎝ of leaves length were highest on soil. Conclusion : The survival rate depending on soil types of Oplopanax elatus was confirmed to exist in these cases of soil and soil+pelite. However, they all died in condition of soil+vermiculite. The number of leaves have no difference and the length of plant and the length of leaves were longest on soil.

Background : The minor saponins produced by the hydrolysis of a major saponins sugar. The minor saponins has high absorption and efficacy compared to major saponin. The acid treatment, heat treatment and fermentation with minor saponin research has been actively conducted. This study was performed in order to investigate the bioconversion of ginsenoside Rg5 of fermented wild ginseng adventitious roots by using lactic acid bacteria. Methods and Results : 20g adventitious roots of ginseng was added to water (10-fold v/w). 10% (v/v) of lactic acid bacteria (Pediococcus pentosaceus HLJG0702[KACC 81017BP]) were inoculated with wild ginseng adventitious roots. For the fermentation process the inoculated samples were transferred to culture room for 1, 3 and 5 days. The fermented samples were dried at room temperature and extracted with 70% ethanol. Extract was concentrated completely at 50 ℃ and Rg5 was analysed by using HPLC. Results showed no significant difference the dry weight of non-fermented and fermented wild ginseng adventitious roots. During the fermentation process, the pH changed from 5.7 to 4.2. HPLC analysis showed higher ginsenoside Rg5 (39.588 mg/g) at 3 days. Conclusion : The fermentation of ginseng root can increase the Rg5 contents and minor saponin composition. This process may be used to enhance the minor saponin thereby increasing in fermented property of wild ginseng adventitious roots.

Background : This study, the fraction for testing the efficacy of the Astragalus extract was concentrated active ingredient. The concentrated fraction was applied to a cosmetic material that Astragalus testing results confirmed that the improved efficacy. Methods and Results : The fractions were performed using an n-butanol solvent for increasing the efficacy of the Astragalus extract, by using the material fractions collected to compare and ultimately an increase in whitening and wrinkle efficacy. The solvent to be used in the fractions was used for the n-butanol good dissolution to the effective substance(Astragaloside, Isoflavonoid). It increased approximately 6.5 times the sample extract and the n-butanol fraction of the Astragalus as a result Astragaloside 15 ppm, 97 ppm respectively analyzed by HPLC equipment, isoflavonoid content was confirmed by an increase in the content of the fractions increased 4.5 times to 280 ppm, 1,260 ppm. Tyrosinase inhibitory effect, respectively IC50 5.70 mg/mL, IC50 1.02 mg/mL to, Collagenase producing ability is IC50 4.88 mg/mL, IC50 0.93 mg/mL with n-butanol fraction was good whitening, anti-wrinkle efficacy than the extract. Sensory evaluation was conducted in the same amount of sample, using a purified Astragalus cosmetics received high marks. Stability evaluation(MTT assay) was checked for more than 100% cell viability at the concentration 2,000 ppm. Conclusion : n-butanol fraction of Astragalus was subjected to a component analysis and In vitro test, it was confirmed an increase active ingredient content. The results of sensory evaluation and stability evaluation, it was confirmed been made to improve qualities as a cosmetic materials.

One of the most effective and safe therapeutic methods for treating vitiligo, mixed autologous keratinocytes (KCs) and melanocytes (MCs) cultures have been used for autologous cell transplantation. However, the present transplantation method is faced with a problem that may require a large amount of skin tissue and keratinocytes have limited culture potency. We have found previously that human adipose derived stromal cells (hASCs) from aspirated fat tissue could be used in place of KCs and sufficient amounts of hASCs for transplantation could be obtained by small amount of aspirated fat tissue. The present investigation was determined the effect of ASCs on ex vivo expansion MCs for transplantation. In addition, we examined for a preservation conditions of MCs which have reported low recovery rates and a slowdown in growth after cryopreservation. Various conditions including ASCs ratio, incubation period, and additive materials for MCs cultivation was determined to improve the expansion ability of MCs. The growth rate of MCs colony was elevated 6.85 folds compared the previous conditions. These MCs showed a specific expression of immature melanocyte protein, Trp-2, but did not express the mature melanocyte proteins and markers (c-kit, CD133, and etc.) of mesenchymal stem cells that represents in ASCs feeder. Results in cryopreservation experiments were determined a preservation medium for MCs showing an increased recovery rates after thawing. The characteristics of MCs after cryopreservation using a designed medium were indicated consistent morphology and immunophenotype. In conclusion, ASCs as a feeder could be used in place of keratinocytes for ex vivo expansion of MCs. For clinical trial for vitiligo patients, efficiency experiments in preclinical state should be followed.