Background : Miscanthus sinensis is a C4 crop with high photosynthetic efficiency and is known as a representative cellulose-based bio-energy crop originating in East Asia such as Korea, Japan, and China. It also has a high resistance to environmental conditions such as low temperature and drying and pests. However, many studies on the antioxidant activity of the M. sinensis have not been conducted. Therefore, this study was conducted to measure total phenol and total flavonoid contents of the M. sinensis accessions collected from some countries and to select the M. sinensis accessions with rich total phenol and total flavonoid contents. The results of this study can be used as basic data for future breeding. Methods and Results : This study carried out the total phenol and total flavonoid contents analysis of M. sinensis collected from different country to preserve genetic resources. Total 205 accessions of M. sinensis were collected from the USA, China, Korea, Japan, Thailand and Zambia. Their leaves were extracted with 100% methanol and concentrated at 45℃. To determine total phenol and total flavonoid contents, we measured the total phenolic content and total flavonoid content. The highest total phenolic conten t(81.04 ± 0.02 ㎎·GAE/g) showed in the accession (PMS-178) from China. The highest total flavonoid content (32.66 ± 0.01 ㎎·QE/g) showed in the accession (KMS104) from Korea. On the other hand the lowest total phenolic content (1.11 ± 0.00 ㎎·GAE/g) and the total flavonoid content (0.81 ± 0.00 ㎎·QE/g) were obtained from the accession (M. sinensis ‘Super Stripe’) from USA. Conclusion : In this study, we analysed the total phenolic and total flavonoid contents by country of origin.

Background : The isoflavonoids, triterpene and polysaccharides were know as major components in Astragalus membranaceus Bunge. Especially, isoflavonoids are classified as ononin, calycosin-glucoside of glycon binding and formononetin, calycosin of aglycone binding. In this study, we performed fermentation by a lot of microorganisms which were derived foods. Methods and Results : The A. membranaceus was extracted with 50% Ethanol and it was concentrated using rotary evaporator. In order to change in isoflavonoids, we fermented the A. membranaceus extracts with microorganisms which have β-glucosidase activity. we chose 20 microorganisms using esculin agar method and three strains with the highest activity of β -glucosidase were identified by pNP assay. The extracts of A. membranaceus were fermented during 3 days in sterilized distilled water, as 1 Brix and 3 Brix. The isoflavonoids of fermented extract, respectively days 0, 1, 2 and 3, were analyzed using High Pressure Liquid Chromatography (HPLC). In addition, the fermented A. membranaceus extracts was investigated about whitening effect using tyrosinase and radical scavenging activity by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). As a result of β-glucosidase activity by pNP assay, we selected two strains, Pediococcus pentosaceus (KACC 81017BP), Saccharomyces cerevisiae (KACC 83014BP). On the third day of fermentation of S. cerevisiae, isoflavone aglycone was the highest. The calycosin-glucoside and ononin in fermented A. membranaceus extract were reduced, calycosin and formononetin was increased compared to control. Also, we confirmed higher activity than control in tyrosinase inhibition rate on the third day of fermentation, as 78.38% and lower IC50 value of radical scavenging activity as 584.68 ㎍/㎖. Conclusion : From the above results, we may suggest that A. membranaceus aboveground parts might have useful as a safe material for functional food and pharmaceutics.

Background : In this study, we investigated the formation of Abeliophyllum distichum callus which has not been reported until now and the culture optimization. The potential of callus cultures was examined by comparing the components and antioxidant activities of callus and wild planting. Furthermore, it was intended to provide data on the possibility of substitution for the active ingredient production of Abeliophyllum distichum callus extraction. Methods and Results : We tried to induce callus by introducing it in vitro culture by using leaves and stem of Abeliophyllum distichum grown in Goesan Chungbuk. In order to investigate the effect of growth regulators on the callus, TDZ, NAA, 2,4-D, IBA and BAP were treated with 0.25 to 5 ㎎/ℓ of single hormone, and the combination hormones were treated with two concentrations of BAP (0.1, 1.0 ㎎/ℓ) and 2,4-D, NAA, IBA at 0.1 ㎎/ℓ and 1.0 ㎎/ℓ. And investigate the effect of inorganic salt concentration on somatic embryogenesis, the incidence of callus was examined by culturing 1X, 1/2X MS medium for 4 weeks. As a result, the effect of growth regulator and treatment concentration on the callus formation of Abeliophyllum distichum, callus induction was the fastest in the 2,4-D condition, but the callus indection was slow. The highest amount was produced ine the NAA condition. The axillary bud was best grown in TDZ condition, but no root was formed. LC chromatogram was able to compare the contents of leaf, stem extracts and callus extracts. Antioxidant ativity showed excellent antioxidative activity in the extracts of the wild planted stem. Conclusion : The optimum culture conditions for callus were 1/2X MS, 1 ㎎/ℓ BAP and 0.1 ㎎/ℓ of 2,4-D. As a result of LC chromatogram, it was confirmed that callus extract contained a large amount of the same components as the stem and leaf extracts grown on the wild planting. Currently, Studies on mass culture using bioreactor to utilize this are underway, We could confirm the potential possibility of Abeliophyllum distichum cultured products.

Background: The objective of this study was to investigate the effect of packaging material on the growth of rootstock of Liriope platyphylla. Methods and Results: This study examined the effects of two types of packaging material, LDPE (low density polyethylene) and functional film on the growth of the tubers of L. platyphylla, at 5℃. During the 16-weeks of storage period, the ratio of loss and decay of the tubers was examined at intervals of 4, 8, and 16 weeks to detect the quality of the plant. After 16 weeks of storage, the treated tubers were own. Subsequently, plant height and the number of leaves were recorded. The results revealed that functional film at 5℃ was the ideal material for the storage of L. platyphylla tubers. The rate of loss was the highest (57.42%) with a onion net and the lowest (22.12%) with a functional film. Similarly, the rate of tuber decay was highest (8.20%) using onion net and the least (4.60%) when the functional film was used. Conclusions: Thus, the use of the functional film proved to be the most effective in the storage of L. platyphylla tubers when compared with the LDPE.

Background : Panax ginseng C. A. Meyer is a medicinal plant corresponding to 'raw materials for food use' in Korean food standards codex, and it is known that it has the same origin although it is classified into wild ginseng, cultured ginseng and red ginseng. It varies morphologically, molecularly, depending on the cultivation or habitat environment. In this study, we carried out experiments for the morphological differentiation of cultured ginseng (Hongcheon, Gunsan, Miryang) and adventitious roots culture of wild mountain ginseng (by company). Methods and Results : Morphological observations were made with resin sectioning method (RSM). The test method was sampled, preprocessed, embedded, segmented, slid and then observed with an optical microscope. The epidermis, cortex and vascular tissues were identified in the inner cross section of adventitious roots culture of wild mountain ginseng. Phloem was formed at the center, and xylem was formed at the side. In cultured ginseng, the organs of the vascular tissues were differentiated into two, whereas in the adventitious roots culture of wild mountain ginseng, one vascular tissue was formed due to the thin adventitious. Conclusion : Plant resources have the diversity of using the same or different origins depending on their distribution. The morphological differentiation method of ginseng and adventitious roots culture of wild mountain ginseng is expected to be based on domestic fair trade to prevent discrimination.

Background : Korea's agriculture has been forced to change due to the decline in agricultural population, the aging of the population and the conclusion of the Convention on Biological Diversity. Thus, agriculture is seeking to develop into a sixth industry. For this reason, it is necessary to find high-function alternative plant resources for health promotion to meet the changes in national needs for agricultural products. The adventitious roots culture of wild mountain ginseng is the raw material of food code that produced by the biotechnology technique. introduced in the culture of the plant, which is the only way to use a raw material of food. Therefore, in order to increase the yield of ginsenoside in adventitious roots culture of wild mountain ginseng, ginsenoside precursor was treated and ginsenoside contents were analyzed. Methods and Results : In order to investigate effect of precursor treatment on the production of ginsenoside in adventitious roots culture of wild mountain ginseng, 5 g of adventitious roots culture of mountain ginseng were cultured in liquid SH media supplemented with 2 ㎎/l IBA, 3% sucrose for 8 weeks, which was co-cultured with β-sitosterol and Squalene (0.0625 to 1 mM) or without. Determination of 17 ginsenosides contents of each extract was carried out by HPLC. Rb3 was accumulated by only β-sitosterol, also it increased production of total ginsenoside in the cultured ginseng roots at a concentration of 0.125 mM, which was 2.47-fold higher than that in the control (78.13 ㎎/g of extract). Conclusion : These results are an important to improve the production yield of ginsenoside in adventitious roots culture of wild mountain ginseng, and they provide an opportunity for development of adventitious roots culture of wild mountain ginseng in dietary health supplement and pharmaceutical industries.

Background : The major components in Astragalus membranaceus are isoflavonoids, triterpene and polysaccharides. Also, isoflavonoids was composed to aglycon and glucoside derivatives. In this study, we performed fermentation process (enzyme treatment) and steam processing (high temperature and pressure) to increase aglycon such as formononetin and calycosin. Methods and Results : The steam processing was performed at a lot of conditions, such as temperature (80, 100, 120℃) and time (30, 60 and 120 min). Fermentation processing carried out by A. membranaceus extract fermented with microorganisms which have high β -glucosidase activity (selection by esculin agar method) for detached glucose from isoflavonoids, converted to algycon. The isoflavonoids were analyzed using high pressure liquid chromatography (HPLC) in fermentation product of A. membranaceu extract by treated with steam processing at various conditions. As a result of β-glucosidase activity by pNP assay, we selected three strains, Pediococcus pentosaceus, Weissella cibaria and Saccharomyces cerevisiae. Isoflavone aglycon was the highest in fermentation product by S. cerevisiae for 3 days, but change of isoflavonoids was not observed in steam processing. The calycosin-glucoside and ononin were reduced in fermentation product of A. membranaceus extract, whereas calycosin and formononetin was increased. Conclusion : This results showed that isoflavone glycoside converted to isoflavone aglycon in A. membranaceus by fermentation process, it seems to be available for industrial use.

Background : This study was carried out to investigate the antioxidative activity and active ingredients of Glehenia littoralis through purification process. Methods and Results : Above-ground and below-ground parts of Glehenia littoralis, dried in Gangneung, were purchased, crushed, sonicated for 2 hours in 100% ethanol, filtered and concentrated. Above-ground part of G.littoralis which is more effective higher antioxidant effect than below-ground part in 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. Was used to extract the above-ground part of the solvent fraction according to polarity (hexane, ethyl acetate, water) and HP20 column separation (0, 30, 60, 100% EtOH). Using the fraction was the DPPH assay with high-performance liquid chromatography (HPLC) analysis. Comparing the antioxidant efficacy with that of fraction isolated from Glehenia littoralis extract. Hexane, water layer and 100% EtOH fraction showed lower efficacy than Glehenia littoralis extract. 60% EtOH fraction showed more than 8 times higher efficacy. In order to compare the components according to their efficacy, HPLC analysis was carried out. The fraction (hexane, water layer, 100% EtOH fraction) which showed low antioxidative activity confirmed imperatoin and nonpolar compound, the fraction (ethyl acetate, 60% EtOH fraction) showed a higher antioxidant activity was confirmed 2 flavonoid and scopoletin. Conclusion : Glehenia littoralis extract showed low antioxidant activity of 893 ㎍/㎖ with IC50 of DPPH assay. However, it showed an increase of antioxidant activity by IC50 of 115㎍ /㎖ of DPPH assay of 60% EtOH fraction by fractionation and separation. Through HPLC analysis, the active ingredient, scopoletin and two flavonoids were identified.

Background : Collagen and elastin contribute to form a network under the epidermis and reduce the wrinkles. Collagen and elastin are degraded by collagenase and elastase. Therefore, inhibition of collagenase and elastase activity could be an excellent method for anti-wrinkle. Pinus Koraiensis leaves contain various flavonoid compounds such as quercetin and kaempferol. According to previous reports, kaempferol has increases procollagen synthesis and inhibitory effect on MMP-1 activity. This study was performed to investigate the anti-wrinkle effect of Pinus Koraiensis leaves extract and fractions. Methods and Results : The active components of Pinus Koraiensis leaves extracts and fractions were determined. Total phenol and total flavonoid contents measured using the Folin-Ciocalteu reagent and according with aluminum chloride colormetric method. The kaempferol content analysis using HPLC system. The total phenol, flavonoid and kaempferol contents of ethyl acetate fraction was the highest. The antioxidant activity measured in according with DPPH, ABTS and RP assay. Pinus Koraiensis leaves extract and factions were the highest ethyl acetate fraction in antioxidant activity. The inhibitory activity on collagenase and elastase of Pinus Koraiensis leaves extract and factions were determined. The results of inhibitory activity on enzymes associated with wrinkle formation was the highest of n-butanol fraction, the next higher was 80% ethanol extract. As a result of using MMP-1 content and procollagen type-I C-peptide content assay kit, the anti-wrinkle effect of n-hexane fraction was highest. Conclusion : These results indicate that Pinus Koraiensis extract may help anti-wrinkl

Background : Korean mountain ginseng adventitious roots culture extract fermentation product (KGEF) is increased the content of low molecular weight ginsenoside Rk1 and Rg5 by purifying, steaming, and fermentation of the wild ginseng adventitious roots culture. In Ministry of Food and Drug Safety, the analysis method of low molecular weight ginsenoside (Rk1, Rg5, Rh2, compound K, etc.) has not been proven, therefore we conducted validation to confirm the suitability of the qualitative and quantitative analysis method for Rk1 and Rg5. Methods and Results : Quantitative analysis was performed at a maximum absorption wavelength of 203 ㎚ (specificity). It was confirmed that the retention time of each peak of Rk1 and Rg5 was separated by chromatogram. The separation degree of Rk1 and Rg5 was 2.15 more as 1.5 a result of calculation according to the formula to evaluate the separation limit. (accuracy). The recovery rate was 101.5% of Rk1 and 103.9% of Rg5 in KGEF. (repeatability). The area value of ginsenoside Rk1 and Rg5 showed high reproducibility with relative standard deviation Rk1 0.86% and Rg5 0.68%. Retention time was also reproducible with relative standard deviation Rk1 of 0.054% and Rg5 of 0.09%. (linearity). The correlation coefficients were 0.999 of Rk1 and 0.999 of Rg5. The reproducibility of retention time in linearity was also high, with relative standard deviation Rk1 0.0017% and Rg5 0.0017% (limit of quantification, limit of detection). The quantitative limit of Rk1 was 53.73 ㎍/㎖ and the detection limit was 17.73 ㎍/㎖ and the detection limit of Rg5 was 259.03 ㎍/㎖ and detection limit was 85.48 ㎍/㎖. Conclusion : In this study, we validated ginsenoside Rk1 and Rg5 for identification and content testing. It will be enables to verify physicochemical differentiation and analytical methods, and to be a research-based data of low molecular weight ginsenosides.

Background: To obtain useful cosmetic resources, this study aimed to determine the non-saponin fatty acid and inhibitory activities of collagenase and elastase by treatment of Saccharomyces cerevisiae in supercritical fluid extracted oil of the adventitious root culture of wild mountain ginseng. Methods and Results: We performed supercritical fluid extraction at various conditions such as pressure, temperature, time, and use of co-solvents, unlike the n-hexane extraction for the adventitious roots culture of wild mountain ginseng. The non-saponin-fatty acid obtained from the oil of the adventitious roots culture was incresed by treatment with S. cerevisiae. The supercritical fluid extraction was conducted using gas chromatography. Non-saponin-fatty acid content, in the oil of adventitious roots culture of wild mountain ginseng treated with S. cerevisiae for 2 days were three times higher than that in the control. In addition, the oil of the adventitious roots culture treated with S. cerevisiae was investigated for the anti-wrinkle effect by using collagenase and elastase. The oil of adventitious roots culture treated with S. cerevisiae exhibited higher collagenase and elastase inhibitory activities than those in the control. Conclusions: Supercritical fluid extracted oil of the adventitious roots culture of wild mountain ginseng treated with S. cerevisiae was found to have decreased ratio of saturated fatty acids and incresed ratio and content of unsaturated fatty acids increased. Furthermore, it showed anti-wrinkle effects in vitro.

Background: Hot steaming is known to be effective in improving the biological activities of plant extracts by breaking down useful compounds to low molecular weight ones. Methods and Results: This study aimed to develop an optimal extraction and steam processing method for enhancing the low molecular ginsenoside contents of the adventitious roots culture of wild mountain ginseng. The total ginsenoside was optimally extracted when 70% EtOH was used at 50℃, whereas low molecule ginsenoside such as Rg2, Rh1, Rh4 and Rk1 could be extracted using 70% EtOH at 70℃. The adventitious roots culture of wild mountain ginseng is known to contain four major ginsenosides, i.e., Rb2, Rb1, Rg1 and Rd, however new ginsenosides Rg6, Rh4, Rg3, Rk1 and Rg5 were new abundantly obtaind after steam processing method was applied. The contents of total ginsenosides were the highest when thermal steam processing was conducted at 120℃ for 120 min. Unlike ginsenosides such as Rg1, Re, Rb1, Rc, Rb2, and Rh1, which decreased after steam processing, Rg3, Rk1, and Rg5 increased after thermal processing. Steam processing significanltly reduced the content of Rb1, increased that of Rg6 by about ten times than that in the adventitious roots culture of wild mountain ginseng. Conclusions: Our study showed that the optimal extraction and steam processing method increased the content of total ginsenosides and allowed the extraction of minor ginsenosides from major ones.

Background : Glehnia littoralis F. and Peucedanum japonicum T. is mostly founded coastal region of Korea, Japan, Taiwan, and China. Recently, because interests of health functional food has been increased the role of miscellaneous medicinal crop is not only strengthen provided energy but also health functional role and many researchers showed interest in plants to in beauty treatment and functional healthy food. The objectives of this research was to analyse antioxidant activity of G. littoralis and P. japonicum. using various plant parts. Methods and Results : Different analysis including DPPH free radical scavenging activity, Total phenolic contents and Total flavonoid content were performed to evaluate the antioxidant activity of different plant part in G. littoralis and P. japonicum. DPPH free radical scavenging activity was calculated a RC50 value (㎍/㎖). Total phenolic contents and total flavonoid contents were expressed as milligrams of gallic acid and quercetin equivalent per gram of dry weight. Respectively, RC50 value of DPPH radical scavenging was higher (900.78 ± 87.53 ㎍ /㎖) in leave of P. japonicum and the lowest RC50 value of DPPH radical scavenging was observed (3806.74 ± 1361.38 ㎍/㎖) in root of G. littoralis. The highest total phenolic level was 5241.46 ± 228.52 ㎎․GAE/g and total flavonoid level was 426.11 ± 37.34 ㎎․QE/㎎ were detected in leaf of G. littoralis. Results showed that DPPH free radical scavenging activity, total phenolic contents and total flavonoid content were higher in leaf of G. littoralis and P. japonicum. Conclusion : Thus, medicinal plants can be an important resource for producing cosmetic and functional health food.

Background : Korean mountain ginseng (Panax ginseng C.A. Meyer) are difficult to industrially apply because of its scarcity and high cost. Advances in plant biotechnology have made it possible to produce mountain ginseng on a large scale using adventitious root cultures in bio-reactors. This study was conducted to develop a cosmetic emulsion using ginsenoside and physiological activity - enhanced raw materials by fermentation process. Methods and Results : Wild ginseng adventitious roots were fermented with Pediococcus pentosaceus HLJG 0702 (KACC 81017BP). ginsenoside contents was analysed by using HPLC. Antioxidant activity was measured by DPPH and ABTS radical scavenging activity and whitening effect was measured by tyrosinase inhibitory activity. After microfluidizer processing was performed to prepare emulsions with homogenized particles, particle size and distribution were measured through a transmission electron microscop e(TEM). Particle stability compares pH, viscosity, light and zeta potential. When fermented with Pediococcus pentosaceus HLJG 0702, the highest change rates of Rg3, Rk1 and Rg5 were shown and the antioxidant activity was increased. The whitening effect was 73.2 ± 0.9% when treated at 100 ㎍/㎖, 1.5 times higher than the control. The optimum particle size and distribution were shown to be 418.0 ± 14.9 ㎚ for 6 times treatment with 0 - 10 times microfluidizer treatment. Stability was about 3% in pH, viscosity and light test. the zeta potential was found to be homogeneous at –33.33 mV. Conclusion : Pediococcus pentosaceus HLJG 0702 Fermented Wild ginseng adventitious roots were found to have effective ingredients and improved physiological activity. We have also developed emulsions that exhibit optimal particle size and distribution

Background : The study about cultured wild ginseng root (Panax ginseng C. A. Meyer) have been reported mainly ginsenosides in saponins family. However metabolites of fermented wild ginseng roots by microorganisms was not reported yet. Methods and Results : Cultured wild ginseng roots were used for fermentation of ginseng roots using Pediococcus pentosaceus and other bacterial strains. We analyzed different types of ginsenoside contents, metabolite and enzyme contents, and gene expression by using microorganisms. Results showed considerable differences in ginseonoside contents specially Rk1 and Rg5. The highest enzyme activity level was by Glutathione reductase (GR) and Glutathione S transferase (GST) in fermented ginseng roots than control (non-fermented), whereas Glutathione peroxidase (GPX) and Peroxidase (POD) contents were reduced. Score plots and loading plots of principal components 1 of the PCA result obtained from the data on 43 metabolites in fermented wild ginseng root of five conditions. The concentration of metabolite such as β-alanin and 4-aminobutyric acid (GABA), which is used to improve memory were increased in fermented ginseng roots than control. We found functional gene in wild ginseng root related with metabolic process. The APX gene expression gradually increased in fermented ginseng root with respect to fermentation times. Conclusion : In this study, accumulation of functional metabolite in cultured ginseng r

Background : The minor saponins produced by the hydrolysis of a major saponins sugar. The minor saponins has high absorption and efficacy compared to major saponin. The acid treatment, heat treatment and fermentation with minor saponin research has been actively conducted. This study was performed in order to investigate the bioconversion of ginsenoside Rg5 of fermented wild ginseng adventitious roots by using lactic acid bacteria. Methods and Results : 20g adventitious roots of ginseng was added to water (10-fold v/w). 10% (v/v) of lactic acid bacteria (Pediococcus pentosaceus HLJG0702[KACC 81017BP]) were inoculated with wild ginseng adventitious roots. For the fermentation process the inoculated samples were transferred to culture room for 1, 3 and 5 days. The fermented samples were dried at room temperature and extracted with 70% ethanol. Extract was concentrated completely at 50 ℃ and Rg5 was analysed by using HPLC. Results showed no significant difference the dry weight of non-fermented and fermented wild ginseng adventitious roots. During the fermentation process, the pH changed from 5.7 to 4.2. HPLC analysis showed higher ginsenoside Rg5 (39.588 mg/g) at 3 days. Conclusion : The fermentation of ginseng root can increase the Rg5 contents and minor saponin composition. This process may be used to enhance the minor saponin thereby increasing in fermented property of wild ginseng adventitious roots.

Background : The study about ginseng cultured roots have been reported mainly ginsenosides in saponins family. Other phytochemical such as non-saponins of fatty acid has been revealed its bioactive activity including anti-oxidation, whitening, anti-cancer. Supercritical extraction (SE) process mainly refer to the extraction with CO2, is usually from a solid matrix, is a sample preparation step for analytical purposes. SE produce no residual solvent and possess high stability of the extract component, which is advantageous for fatty acid analysis. Methods and Results : Fermented ginseng cultured roots used in the experiment were used for fermentation using Pediococcus pentosaceus. SE performed at different temperature, pressure and extraction time using non-fermented and fermented ginseng roots. Further we fractionated from fermented ginseng using Methanol, Hexane, Ethanol, Ethyl acetate and Butanol. We compared fatty acids contents ginseng extractions by GC analysis. Methyl linoleate contents was 44% of fatty acids supercritical extraction contained. The contents of Methyl linoleate was the most dominant component among 37 types of fatty acids by SE and other extractions solvent. Total fatty acids contents obtained by SE process from fermented ginseng (1325.61ppm) was twice than from non-fermented ginseng (618.47ppm). Conclusion : Fatty acids contents by SE was increased at high pressure. The best condition for fatty acids contents extraction was 60℃, 350bar and 3h.

Background : This study, the fraction for testing the efficacy of the Astragalus extract was concentrated active ingredient. The concentrated fraction was applied to a cosmetic material that Astragalus testing results confirmed that the improved efficacy. Methods and Results : The fractions were performed using an n-butanol solvent for increasing the efficacy of the Astragalus extract, by using the material fractions collected to compare and ultimately an increase in whitening and wrinkle efficacy. The solvent to be used in the fractions was used for the n-butanol good dissolution to the effective substance(Astragaloside, Isoflavonoid). It increased approximately 6.5 times the sample extract and the n-butanol fraction of the Astragalus as a result Astragaloside 15 ppm, 97 ppm respectively analyzed by HPLC equipment, isoflavonoid content was confirmed by an increase in the content of the fractions increased 4.5 times to 280 ppm, 1,260 ppm. Tyrosinase inhibitory effect, respectively IC50 5.70 mg/mL, IC50 1.02 mg/mL to, Collagenase producing ability is IC50 4.88 mg/mL, IC50 0.93 mg/mL with n-butanol fraction was good whitening, anti-wrinkle efficacy than the extract. Sensory evaluation was conducted in the same amount of sample, using a purified Astragalus cosmetics received high marks. Stability evaluation(MTT assay) was checked for more than 100% cell viability at the concentration 2,000 ppm. Conclusion : n-butanol fraction of Astragalus was subjected to a component analysis and In vitro test, it was confirmed an increase active ingredient content. The results of sensory evaluation and stability evaluation, it was confirmed been made to improve qualities as a cosmetic materials.