Traf4 (Tumor necrosis factor Receptor Associated Factor 4) is a member of the tumor necrosis factor receptor (TNFR) - associated factors (TRAFs) family. TRAF4 is overexpressed in tumor cells such as breast cancer and associated with cytoskeleton and membrane fraction. Interestingly, TRAF4 was localized with tight junctions (TJs) proteins including OCLN and TJP1 in mammary epithelial cells. However, the expression patterns and biological function of Traf4 were not examined in preimplantation mouse embryos although Traf4-deficient mouse showed embryonic lethality or various dramatic malformation. In this study, we examined the temporal and spatial expression patterns of mouse Traf4 during preimplantation development by qRT-PCR and immunostaining, and its biological function by using siRNA injection. We found upregulation of Traf4 from the 8-cell stage onwards and apical region of cell – cell contact sites at morula and blastocyst embryos. Moreover, Traf4 knockdown led to defective TJs without alteration of genes associated with TJ assembly but elevated p21 expression at the KD morula. Taken together, Traf4 is required for TJs assembly and cell proliferation during morula to blastocyst transition.

The Spratly Islands dispute is an ongoing territorial dispute between China, Taiwan, Malaysia, the Philippines, Vietnam and Brunei, concerning territorial sovereignty over the Spratly Islands. This conflicting territorial claim between these coastal parties is raising tensions in Asia, so the settlement of this dispute is of key importance for a peaceful atmosphere in the area. The dispute is also significant in respect of being an international geo-strategic, economic, political and legal matter. After a 1988 armed conflict between Vietnamese and Chinese forces, the claimants have looked for approaches to solve the conflict peacefully through different informal endeavors, but due to the complexity of the dispute there are a number of barriers to reaching a permanent settlement. This study puts forward some potential approaches for resolving the dispute, considering its complex nature, by evaluating the six parties’ competing claims and analyzing the legal soundness of their claims.

With continuous development in the field of sample preparation technology, solid phase micro-extraction (SPME) has been widely used in analytical chemistry for high extraction efficiency and convenient operation. Different materials lead to different extraction results. Among existing materials, carbon-based materials are still attracting attention from scientists due to their excellent physical and chemical properties as well as their modifiable surfaces, which could enhance the adsorption effects of SPME fiber. This review introduces the preparation methods and applications of different kinds of carbon-based material coatings on fibers. In addition, directions for future research on carbon material composites are discussed.

Based on the inner-effect mechanism of transport infrastructure and regional economic growth, this paper builds a specialized spatial weight matrix by utilizing the panel data from 31 provinces in New Silk Road Economic Belt (NSREB) and other areas from 2005 to 2014, and combines with the spatial panel model to analyze the spatial spillover effects of transport infrastructure. According to the analysis, the transport infrastructure plays an obvious lead role in regional economy growth alongside the NSREB, and the economic growth invigorates common development in surrounding regions. In addition, differences were observed among the different transport infrastructure with regard to their influences on regional economic development, as the highway transport affects regional economic growth to a larger degree than railway transport.

Along with the drastic competition among ports, the strive for the vast economic hinterland and the supply of goods have become strategic problems for port operators. At the same time, port enterprises are paying more and more attention to the construction of dry ports. This article establishes the port cooperation performance evaluation index based on the balanced score card method and uses the grey relational degree method to evaluate the cooperation performance between seaports and dry ports. Finally using Qingdao port and Xi'an port as an example, and Zhengzhou port and Lanzhou port as benchmarks, the application of this evaluation method is introduced in detail. The conclusion reveals that cooperation between Qingdao port and Xi'an port has deficiencies in customer satisfaction, financial cooperation and non-market tools. Alongside this, the author proposes related issues about information management in the supply chain, competition position and the scope of hinterland. This article, combined with the related theory of supply chain and performance evaluation, puts forward a set of relatively complete cooperation performance evaluations between seaports and dry ports, which provide scientific theory support for better cooperation.

Since 1972, the《Convention on the protection of the world cultural and natural heritage》has been signed,and the concept of world cultural heritage has been defined. New types of heritage continue to be discovered and emerge.Cultural landscape, linear heritage, heritage canal, the memory of the worldheritage, world agricultural heritage, the wetlands in the world heritage, intangible cultural heritage, historic towns and urban centers, industrial heritage and heritage of the twentieth Century etc. This is a reflection of social progress,and is also closely related to the "common" spirit and idea based on value protection which isembodied and advocated in the convention .This basic consensus promotes the great development of heritage protection work over the world.

Focuses on discussing the shapes, characteristics and constructions of the traditional ondol in northern China,totapthereasons for its forming characteristics. After comparing China's traditional ondol culture with modern living space, we want to show the relationships of dimensional processing patterns appearing at different historical times.

In this paper, the Beijing courtyard and Fujian Earth Building--model for two typical entry points, through both a comparative analysis of the typical form and from the perspective of traditional Chinese culture to explain the differences between the two shapes in order to explore the south and the north of China's residential cultural similarities and differences .

The particulate strengthened composites were prepared by a PM process to develop novel copper based composites with reasonable strength, high thermal conductivity and low thermal expansion coefficient. Microstructure of the composites was investigated by SEM; the tensile strength, elongation, thermal conductivity and thermal expansion coefficient (CTE) of the composites were examined. A comparative analysis of mechanical and thermal properties of various Cu-matrix composites currently in use was given and the strengthening mechanisms for the composites were discussed.

Purpose: The purpose of this paper is to empirically investigate the relationship between government subsidies and research and development (R&D) investment of animal husbandry companies in China. The moderating effects of firm size, debt ratio, and firm profitability on this relationship are also examined. Research design, data and methodology: The analysis is based on 14 animal husbandry companies listed on the Shanghai and Shenzhen stock exchanges over the period of 2012-2016. Data are obtained from the China Stock Market & Accounting Research (CSMAR) database and the RESSET database, and multiple regression analysis is utilized with the aid of Stata. Results: The empirical results show that government subsidies can promote R&D investment of animal husbandry companies in China. In addition, firm size, debt ratio, and firm profitability have positive moderating effects on the relationship between government subsidies and R&D investment. Conclusions: Based on the results, the paper concludes that government subsidies play an important role in the process of R&D of China’s animal husbandry companies. This paper recommends that managers of animal husbandry companies should enhance the utilization efficiency of government subsidies and put great emphasis on R&D investment. The policymakers should implement more incentives to encourage animal husbandry companies to invest more in R&D.

Purpose - The study on the consumption structure of urban residents can help us to understand demand law and to grasp the changing consumption trend of people. Consumption structure is an important indicatorreflecting the people’s living standard. It is of realistic significance to study urban consumption structure. Research data and methodology - This study is carried out with data connected with urban residents from Shandong Statistical Yearbook for the period 2000-2010 analyzing eight commodity groups. The almost ideal demand system (AIDS) is one of the important models related to consumption structure. Results - This paper shows that firstly gives a brief introduction to AIDS. Then it makes an empirical analysis on the urban residents’consumption structure in Shandong province, China on the basis of AIDS model. Conclusions - the authorities are supposed to control the prices of HC, Foodstuff and Housing and encourage the consumption of HC, Housing, EE accordingly. At the same time, local government should increase the supply of goods connected with housing, HA, HC, and EE so as to attract more consumption from the urban residents in Shandong.

South Korea has made remarkable achievements and gained many experiences on waste disposal and recycling, which help our country to develop cyclic economy and construct a harmonious society. This paper summarizes the policies, regulations and specific practices on waste disposal and recycling including industrial waste and household waste in South Korea so as to provide some references for the establishment of policies related to the waste recycling in our country.

This paper is a study of the relationships between trade and transshipment in Korea. Through the analysis of the data collected, a comprehensive model has been developed to analyze and predict relationships between trade and transshipment. By using analyses of port and trade evolution in Asia, the model identifies some important results. An application of the model to forecast developments in selected regions in China is also included in this paper. The paper provides a basis for shipping companies to decide on appropriate transshipment port strategies, and provides important theoretical references for Korean ports' development Supported by Shanghai leading Academic Discipline Project, project Number: T0602.