Tetranychid mites are one of the most diverse group including at least 1,200 species in the world. Species identification is difficult due to the small size and similar morphology within the group. We collected 17 species of spider mites from various host plants in different regions of Korea and determined species identity by the comparison of morphological characters and nucleotide sequences of internal transcribed spacer 2 (ITS2) and the cytochrome oxidase subunit I (COI). In addition, we report three new species that were firstly identified in Korea. Amphitetranychus quercivorus (Ehara and Gotoh) was collected from Mongolian oak plant in Daegu, Schizotetranychus miscanthi Saito was from the common reed plant in Ulleungdo, and S. cercidiphylli Ehara was from Bamboo plant in Jeju. Morphological identification of three species were similar with those of Japanese samples, but the ITS2 and COI sequences of A. quercivorus and the COI sequence of S. miscanthi were different with Japanese species at the rates of 1/419, 2/331 and 3/332 nucleotides, repsectively. S. cercidiphylli can be identified by the aedeagus shape of males but we firstly sequenced ITS2 and COI of this species. Our results can be used for the identification of spider mites which are important in plant quarantine.

Spider mites are one of major pests in cultivations of various ornamental plants and also important in plant quarantine service. Due to the very small body size and high similarity within the Genus the identification of species is difficult even at the microscopic observation. To identify 5 major species (Tetranychus cinnabarinus, T. urticae, T. phaselus, T. kanzawai and T. truncatus) in the Genus Tetranychus at the molecular level, we designed 4 species-specific primer sets using nucleotide sequences of the internal transcribed spacer (ITS2) region in the nuclear ribosomal repeat unit. At the PCR diagnosis of extracted genomic DNAs of 5 species using each primer set, specific primers of both T. phaselus and T. truncates were species-specific to their own species samples. However, specific primer set of T. urticae detected T. cinnabarinus as well as T. urticae. Specific primer set of T. kanzawai detected T. truncates as well as T. kanzawai, even though detection intensities were lower in non-target species.

Due to the very small body size of spider mite, it is difficult to prepare DNA extraction simultaneously with slide samples from a single individual. Here we developed non-mashed DNA extraction method from a single spider mite to apply for molecular as well as morphological identification. Total genomic DNA was isolated from a single female adult using Genomic DNA extraction kit without the sample homogenization. DNA content of a single spider mite was 60-90 ng, which is sufficient for the PCR analysis. However, the quantity of extracted DNA and quality of the cuticle sample were dependent on the incubation time into the lysis buffer. Our results suggest that non-mashed DNA extraction method would be useful for the identification of very small mites as well as insects at the levels of DNA and morphology.

The role of Cl channels in regulatory volume decrease (RVD) in human salivary gland acinar cells was examined using a whole-cell patch clamp technique. Human tissues were obtained from healthy volunteers or from patients with oromaxillofacial tumors. During the measurements, K+-free solutions were employed to eliminate contamination of whole-cell conductance by K+ currents. When the cells were exposed to a 70% hypotonic solution, outward-rectifying currents, which were not observed in the resting state, were found to have significantly increased both in human labial and parotid gland acinar cells. The amplitudes of the currents were reduced in a low CI bath solution. Furthermore, the addition of 100μM 5-Nitro-2- (3-phenyl propylamino) benzoic acid (NPPB) or 100μM 4,4'-diisothio cyanatostilbene-2,2'-disulphonic acid (DIDS), known to partially block Cl channels, significantly inhibited these currents. Its outward-rectifying current profile, shift in reversal potential in a low Cl bath solution and pharmacological properties suggest that this is a Cα2+ independent, volume activated Cl current. We conclude therefore that volume activated Cl channels play a putative role in RVD in human salivary gland acinar cells.

The mites of the superfamily Eriophyoidea are of worldwide distribution, and invariably associated with plants. They are extremely small in size with average 200 ㎛ of adult body length ranging from 80 to 500 ㎛, and thus often invisible to the naked eyes without noticeable deformation and/or injury on the host plants. Their general appearance is rather worm-like, with an elongated and transversely annulated opisthosoma, and with only 2 pairs of legs having a empodial featherclaw instead of paired true claws. The extremely small size and secret habits of eriophyoid mites cause them to be ignored or overlooked, and make them little understood and appreciated, although they are obligatory plant feeding mites being distributed in most botanical biosphere of the world. Nevertheless, some species are of considerable economic significance as the tomato russet mite (Aculops lycopersici), Citrus rust mite (Aculops pelekassi), pear leaf rust mite (Phyllocoptes pyrivagrans), Castanopsis witches’ broom mite (Aceria kadonoi), Retusa fringe tree malformation mite (Aceria sp.) calls for a greater awareness of them in Korea. Besides, the eriophyoid mites from ornamental trees get to known to public, since the trees are planted along a street. Information on eriophyoid mites in Korea is often difficult to obtain. This was mainly due to the lack of taxonomic works done in Korea. Furthermore, some records on eriophyoid mites are not taxonomically based but just adopted neighboring countries’ records. Their original descriptions are frequently inadequate with focusing on the ecological aspects rather than taxonomic ones. So, some species including Aceria species from Lycium chinensis, in the light of recent knowledge of eriophyoid taxonomy, should be renamed and reclassified. Until now, more than 38 species in 17 genera of the family Eriophyidae have been reported in Korea, which is still relatively small number compared with those in neighboring countries; China (197 spp., in 60 gen), Japan (47 spp., in 19 gen), and Taiwan (75 spp., in 41 gen). Furthermore, none of the other families (Phytoptidae and Diptilomiopidae) have known yet. There is little doubt that more species await discovery in Korea. Considering the geographic distribution patterns, only 7 species among 38 species of Korean eriophyids are endemic. A total of 23 Korean eriophyid species are shared with Japan, 12 species with China, 5 species with Taiwan, 7 species with Russia, and 19 species with USA. It shows that the Korean eriophyid fauna seems to be similar to the Japanese fauna zoogeographically, and more cooperation between Japanese and Korean taxonomists is continuously needed in the eriophyoid field. On the other hand, more endemic and taxonomically important species are expected to be found in the Korean peninsular, since the eriophyoid mites is highly host specific and Korean peninsular is one of the most interesting areas with intense speciation processes. The upland and mountainous landscape (constituting 80% of the peninsula) favours the existence of multiple isolated habitats. The Korean peninsula is characterised by a high level of endemism among the species of higher vascular plants. Out of 2,898 plant species 407 are endemics (14%), including 224 endemic species in the southern and 107 endemic species in the northern part of the peninsula.

Osteoclasts are multinucleated cells with bone resorbing activity and differentiated from hematopoietic cell lineages of monocyte/macrophages in the presence of receptor activator of NF-xB ligand (RANKL) and M-CSF. However, the exact molecular mechanisms through which RANKL stimulates osteoclastogenesis remain to be elucidated. Here we report that activation of cAMP-response elementbinding protein (CREB) is not involved in osteoclastogenesis from osteoclast precursors in response to RANKL. RANKL induced CREB activation in osteoclast precursors. Using pharmacological inhibitors, we found that RANKL-induced CREB activation is dependent on p38 MAPK pathways. We also found that ectopic expressions of wild type and dominant negative forms of CREB in osteoclast precursors did not affect RANKL-induced osteoclast formation and bone resorbing activity. Furthermore, dominant negative forms of CREB did not alter the expression levels of osteoclast-specific marker genes. Taken together, these data suggest that CREB is dispensable for differentiation and resorbing activity of osteoclasts.

Purpose – The portability, functionality, and convenience of smart phones are constantly updated. With the rapid popularization of users of mobile terminals, Xiaomi is also developing rapidly. In February 2015, the users of Xiaomi exceeded 100 million people. As a transnational industry, Xiaomi has developed rapidly in not only China but also Korea. However, through the literature review, there is no radmissible study on the Xiaomi mobile telephones in the Korean market, so it is necessary to study the Xiaomi mobile phones in Korean market. Research design, data, and methodology – Figure analysis of data and social science analytical software of IBM SPSS AMOS 23.0 and IBM Statistics 23.0 were used for all the data researched. Results – First, the innovative diffusion temperament and the compatibility of Xiaomi have positive impacts on achievement expectations and effort expectations. Second, the innovative diffusion temperament and the complexity of Xiaomi have negative impacts on achievement expectations and effort expectations. Third, the innovative diffusion characteristics and the relative superiority of Xiaomi have positive impacts on achievement expectations and effort expectations. Conclusions – Through the analysis of the prior study, the innovation acceptance characteristics consist of compatibility, complexity, relative superiority, observation possibility, and the attempt possibility; the technical acceptance characteristics consist of achievement expectations, effort expectations, social influence, promotion condition, the study conducts relevant research on the continued use intention and analyze the hypothesis of research model.

Purpose – The purpose of this research is reflected on the rapid development of online tourism industries. The study was to establish the strategy for Korean tourism enterprises to develop tourist commodities suitable for Chinese tourists and attract them to visit Korea by the empirical analysis of the relation between repurchase intention of tourists and its premise variables (e-service quality, perceived value and satisfaction). Research design, data, and methodology – This research carried out a questionnaire survey on Chinese tourists who visited Korea with experience of using the online travel agency web sites. A total 398 answers were recovered, 41 of them were excluded due to the dishonest answers and 357 of them were finally analyzed. The data was analyzed with IBM SPSS AMOS 22.0. Results – The research results show that in the online travel agency web site e-service quality, convenience, interactivity, information validity, credibility had a positive impacts on perceived value and satisfaction. The perceived value of online travel agency website users has positive impart on satisfaction and repurchase intention. Satisfaction of online travel agency web site users have positive impacts on repurchase intention. But safety has no impact on perceived value while positive impacts on satisfaction was affected. Conclusions – First, in the online travel agency web site e-service quality, safety has no impact on perceived value while it was shown to have positive impacts on satisfaction because the users of online travel agency web sites believe that the protection of personal information, the defense of cracker and the safeguard of payment security are the basic premises of website operation. Although safety does not have impacts on perceived value, users benefits will suffer damage when hacker intrusion and other accidents occur so that online travel agency web sites should not ignore the security concerns. Second, credibility is a major concern for online travel agency web site users. At this time, it is necessary for the web site to establish a system to display both the commodity information and the using experience published on the user's SNS, thus improving the credibility of the website information.

Purpose - Based on preceding studies, this thesis focuses on the finding of the definition and category of mobile tourism application and deriving out its characteristics. And after looking for how they make influences on continuous intention to use, we make empirical study with TAM model. Research design, data, and methodology - There are many Chinese tourist who visit Korea with user's constant intention to use of tourism application. This study is to find out the definition and category of mobile tourism application through research of preceding study and to fomulate the research model and hypothesis that how tourism application attributes (convenience, interaction, accessibility, local basis, security) affect constant intention to use of mobile tourism application. In order to verify a hypothesis, we conducted a survey for Chinese users of tourism application. In empirical study, we analyzed a structure model for frequency analysis, reliability analysis, exploratory factor analysis, validity analysis through IBM SPSS Statistics 21.0 and IBM SPSS AMOS 21.0 Results - Among tourism applications, convenience, interaction, accessibility and local basis have positive effects on both perceived usefulness and perceived easiness respectively. But security does not. Also perceived easiness has a positive effect on perceived usefulness. Finally, perceived usefulness and perceived easiness have positive effect on constant intent to use. Conclusions - Tourism application enterprises should put emphasis on design such as menu or function in order to simplify the operation of new services for new customers. Therefore, comfortable user interface and development of useful function can improve tourism application. Consequently, it leads to the promotion of tourism application. Also, when users perceive tourism application as a useful media which is easy, comfortable and useful content, the degree of constant intention to use becomes increased. It is important to provide plentiful and useful contents for customers and to develop user interface such as easy operation because these factors have positive effects on constant demand and use of tourism application.

Purpose - Through the analysis of the characteristics of overseas direct purchase websites and the relevant empirical analysis of the quality of service delivery, the activated theory/strategy and significance of the overseas direct purchase are sorted out. Research design, data, and methodology - In the process, to make more effective analysis, several analysis tools and analysis programmes are used, IBM SPSS Statistics 23.0 and IBM SPSS AMOS 23.0. Results - Among the characteristics of the overseas direct purchase website, the security, convenience and information provided by the overseas direct purchase have already had a positive impact on the satisfaction. The quality of the delivery service is composed of four factors: rapidity, reliability, intimacy and correctness. The research results indicate that the satisfaction level has a positive impact on the re-purchase intention. Conclusions - The significance of this study has the characteristics of overseas direct purchase sites which have confirmed that security convenience and intelligence availability have a positive impact on satisfaction and re-purchase intent. It is important to have a safe and reliable access to shopping sites on overseas direct purchase sites. In the characteristics of overseas direct purchase sites, the interaction have no positive effect on satisfaction and re-purchase intention.

Purpose – Before the economic reforms, the Chinese communication industry was poorly developed. After China’s entering to WTO, the Chinese market were gradually opened, domestic companies will be faced with more and more pressures of competition from the world rival countries. As time goes on, the overseas telecommunications companies will occupy the Chinese market with the opening of China Telecommunications market to outside. So this paper focuses on problems and development methods based on the research about the present situation and development methods of communication industry(communication manufacturing industry and communication manufacturing industry) in China. Research design, data, and methodology – This study analyzed the policies of China’s entering to WTO. This paper observed previous researches and made an empirical research on Chinese communication industry based on the governmental policies, strategies, books and previous papers with several kinds of clear data announced formally by China authorities. Results – Most recently, reorganization of the communication industry has brought good opportunities for the development of the communication manufacturing enterprises. This paper analyze policy changes of Chinese communication industry, the status of communication manufacturing industry and communication service industry. Finally, this study for further research analyzes the existing problems and puts forward some practicable measures to solve them. Conclusions - Looking ahead, with China’s rapid economic development and steady deepening of reform and opening-up, the Chinese communication industry is faced with an even broader prospect of development. Chinese communication industry will be become the pillar one in national economy after 10 years development. Foreign communication companies accelerated investment and progresses to Chinese information and communication markets. Positive ones are more foreign investment, export increase, domestic innovation, communication industry made steep growth. But negative ones are obstacles of domestic companies' development and jobless rate increase etc. Second communication manufacturing industry made good development, but computer and TV related industry made decline in growth. Third, market sizes of internet and mobile services are growing but the size of wired communication services is downsizing gradually. To overcome them, the studies of components or parts of communication manufacturing industry are needed individually. Second China Unicom, China Telecom, China Mobile etc. are Chinese representatives. The sales volumes are very similar at the beginning, but now they are different and make big differences. So the analysis about, their differences and its impact, are needed.

Purpose - The purpose of this article is to examine the effects of perceived value, satisfaction, and continued use intention of e-service Quality of taxi-booking apps. Research design, data, and methodology - The questionnaire was created to examine every relevant variables to practical and theoretical implications. The pilot survey was conducted for 15 days from April 7th to April 21st in 2016. Their total numbers were 354 surveys. But 330 copies were used for the analysis except 24 of them. To make a more effective analysis, several analysis tools and analysis programmes were used, such as IBM SPSS and AMOS. Results - The results are as follows. First, all variables of e-service quality except safety make influences on perceived value. Second, e-service quality except safety makes influences on satisfaction. Third, perceived value makes influence on satisfaction and on intention of continued use. Fourth, satisfaction makes influence on intention of continued use. Conclusions - First, 5 variables are very limited. more valuable variables are need to test with this model. Second, this study was conducted only in China. So there may be possible to the representatives. To use this model for better analysis and application abroad, more characteristics and strategic factors should be considered like the local culture of a particular country, attributes for different environment etc.

Purpose - The purpose of this study is to grasp the concept, characteristics and application status of sharing economy, and to derive a research model based on sharing economic service, and to analyze factors and influences of consumers' intention to reuse of sharing economy. Research design, data, and methodology - The questionnaires were created to examine variables for practical and theoretical implications. After pilot survey, conducted for 24 days from March 10th to April 2st in 2017, total numbers were 377. But 330 copies were used for the analysis with IBM SPSS Statistics 23.0 and IBM SPSS AMOS 23.0. The structural equation model was applied for this. Results - First, sharing economic services remain at an early stage, but it is meaningful to identify the revenue mechanism of the business model of the sharing economic platform. Second, in this study, it is meaningful that we systematized the theoretical structure by examining existing studies on the characteristics of the sharing economic service and consumer characteristics, and by examining empirically. Third, Satisfaction and Reliability are related to the characteristics of Sharing Economic Service (Security, Convenience, Discount, Sharing, Social Interaction), Consumer Characteristics (Personal Innovation, Word-of-Mouth) It is meaningful to broaden the understanding of the factors by verifying the mediating effect. Fourth, the sharing economy business is meaningful in that it is a new consumption trend that changes the meaning of consumption to consumers. Gradually, more and more people are recalling that purchasing something is not consumption, but sharing and borrowing is also consumption. In other words, through the sharing economy, consumers can experience more products and services, have more choices, and are expected to have a positive impact on economic growth by increasing the utilization of idle resources. Conclusions - Currently, the sharing economy is growing rapidly all over the world. Therefore, in the subsequent study, it is necessary to compare Korea and China's sharing economy and study the cultural and social characteristics of Korea and China. In particular, I think that steady research is necessary for more precise and specific direction on the influence of the shared economy.

Purpose - Because of the development of smartphone and communication technology, Smart TV programs are filled with various contents and applications. This study included additional individual variables like innovativeness and self-efficacy, and characteristics of smart TV are both user-interface and function as exogenous factors based on TAM model. So, this study focuses on identifying the influencing factors of continuous use intention of smart TV traits and user ones and analyzing how they make influences on them in China market. Research design, data, and methodology - Totally 182 samples were adopted as appropriate ones for analysis in this study. They were collected from 20 February 2016 to 10 March 2016. Results - The results are as follows. First, function has positive influence on perceived usefulness. Second, innovation and user-interface make affirmative influences on perceived easiness. Third, perceived easiness has affirmative influence on perceived usefulness. Fourth, the perceived easiness and perceived usefulness make positive ones on continuous use intention. Fifth, perceived ease of use affects significantly on perceived usefulness. Conclusions - According to the findings, they (smart TV traits and user traits), influencing on continuous use intention, are possible to give significant implications on persistent development in China smart TV market.

Root-knot nematode, Meloidogyne incognita is a virulent pest of solanaceaous crops worldwide. The M. incognita resistance gene Me7 derived from Capsicum annuum CM334, is located on chromosome 9. In the present study, an F2 population derived from a cross between ECW03R and CM334 was used to locate the Me7 gene. An F2 population was inoculated using approximately 1,000 second-stage juveniles per individual plant. Phenotype screening was done 45 days after inoculation by using gall index system. The phenotype study of 503 F2 individual showed 391 resistant and 112 susceptible plants. The 3:1 phenotypic ratio confirmed that resistance phenotype is controlled by a single dominant gene. Previously reported two markers were tested to reveal the linkage of markers to phenotype. Two markers, CAPS_F4R4 and SCAR_PM6a were located at 4.3 and 2.7 cM from the resistance gene, respectively. Additional SNP markers were developed using CM334 reference genome information to narrow down the position of the gene, but no closer markers could be developed due to errors of DNA sequence assembly. The closest marker was positioned on telomere of the chromosome 9 long arm, where tens of other NB-LRR genes are clustered. NB-LRR genes are being used as candidates to identify the Me7 gene.

Soybean can produce high-N residue due to N-fixation, so soybean rotation may increase yield of subsequent corn and reduce N fertilizer on the corn fairly. To find out the contribution of nitrogen to subsequent corn following soybean cultivation, soil nitrate, corn yield, and nitrogen uptake were measured for three continuous corn cropping years after soybean rotation. Three N rates of 0, 80, and 160 kg/ha were applied to three continuous corn following soybean cropping. At 6-leaf stage, soil nitrate amount at the soil depth of 0-30cm ranged from 60 to 80 kgN/ha higher in the first corn cropping year than that in the second and third corn cropping years. Judging from corn N status such as SPAD value, N concentration of ear-leaf and stover at silking stage, N contribution of previous soybean to corn in the first corn year was N fertilizer of approximately 80 kg N/ha. Stover N uptake at silking stage increased from 47 to 52 kg N/ha at the 0, and 80 kg N/ha of N rates in the first corn cropping year compared with those in the second and third corn cropping years. Corn grain yield at the 0 kg N/ha of N rate was 6-7 ton/ha higher in the first corn cropping year than that in the second and third corn cropping years, respectively. When compared the first corn year following soybean cropping with the second and third corn cropping years, N uptake of grain and stover at harvest with low N rates such as 0 and 80 kg N/ha increased from 45 to 67kg N/ha, from 35 to 60 kg N/ha, respectively. N uptake of whole plant by soybean rotation increased from 93 to 118 kg N/ha in the first year compared with that in the second and third corn cropping years. However, the N contribution by soybean cropping was small in the second and third continuous corn cropping years. Therefore, it was concluded that the nitrogen fertilizer of 80-100 kg N/ha in the first corn cropping year could be saved by soybean rotation and annual alternative corn-soybean rotation could be the best rotation system