The Republic of Korea is expected to participate in the denuclearization verification activities by the International Atomic Energy Agency (IAEA) in case any neighboring countries declared denuclearization. In this study, samples for the verification of nuclear activities in undeclared areas were selected for the denuclearization of neighboring countries, and the appropriateness of the procedures was considered. If a country with nuclear weapons declares denuclearization, it must be accompanied by the IAEA’s verification regarding nuclear materials and weapons in the declared and undeclared areas. The analysis of the process samples or on-site environmental samples and the verification of undeclared nuclear facilities and materials aid in uncovering any evidence of concealment of nuclear activity in undeclared areas. Therefore, a methodology was established for effective sampling and analysis in accordance with proper procedures. Preparations for sampling in undeclared areas were undertaken for various potential scenarios, such as, the establishment of zones according to radiation dose, methods of supplying electricity, wireless communication networks, targets of sampling according to characteristics of nuclides, manned sampling method, and unmanned sampling method. Through this, procedures were established for pre- and post-site settings in preparation for hazards and limiting factors at nuclear inspection sites.

In this study, the layered structures of immiscible Fe and Cu metals were employed to investigate the interface evolution through solid-state mixing. The pure Fe and Cu powders were cold-consolidated by high-pressure torsion (HPT) to fabricate a layered Cu-Fe-Cu structure. The microstructural evolutions and flow of immiscible Fe and Cu metals were investigated following different iterations of HPT processing. The results indicate that the HPTprocessed sample following four iterations showed a sharp chemical boundary between the Fe and Cu layers. In addition, the Cu powders exhibited perfect consolidation through HPT processing. However, the Fe layer contained many microcracks. After 20 iterations of HPT, the shear strain generated by HPT produced interface instability, which caused the initial layered structure to disappear.

본 연구는 지속가능한 유기농업의 실천을 위하여 국내 작부체계와 농업환경에 적합한 한국형 현장적용 기법을 구축하고자 수행하였다. 본 연구의 기본 개념은‘Natural Enemy in First (NE가 먼저)’로 해충 발생시기의 예찰 없이 주 작물을 정식함과 동시에 천적과 선발한 보조식물을 혼합 적용해서 해충발생 이전에 천적을 포장에 먼저 정착시키는 생물적 방제기법이다. 미끌애꽃노린재 서식처로 Portulaca sp.를, 콜레마니진디벌 서식처로 옥수수를 선발하여 시설 토마토에서 방제효과를 확인하였다. 미끌애꽃노린재 단독처리와 천적 서식처(Portulaca sp.) 혼합처리구에서 관행방제 처리구(약제처리)대비, 각각 82%, 73%의 총채벌레 밀도 억제 효과를 확인할 수 있었다. 진딧물의 경우, 모든 처리구에서 3줄기 당 평균 0.5마리 이하의 낮은 밀도를 유지하였다.

Space law is normally referred to international space law. As national space activities develop, however, national space laws have been legislated in many countries for the development of space resources. These are used to present conflicting cases between national and international space law (corpus juris spatialis internationalis) on the interpretation of space resource exploitation. This study is devoted to bridging the gap between these two legal systems. In this paper, the author will critically review the fundamental principles of space resource exploitation under international law and suggest a direction for setting up national space laws for future space resources. This paper is composed of seven parts, including a short Introduction and Conclusion. Part two will discuss acts pertaining to asteroid resources. Part three will deal with res extra commercium. Part four will analyze the non-appropriation principle. Part five will look into the common heritage of mankind. Part six will investigate res nullius humanitatus.

The composition of the deicer sprayed on the highway is spreading over the highway by the scattering or snow removal activity, or car movement and consequently affecting the vegetation environment around the highway. These are the cause of the damage of fruit trees and crops, and also the cause of corrosion of highway structures. The goal of this study is to estimate the detected range of deicer components from a highway. The concentration of the deicer components in gauze and soil were investigated according to the crossing distance from the highway. The data collected were then used to estimate the concentration range of deicer components in a rage of distance up to 100 m from the highway where the deicer was spread. The sample time and weight of gauze were measured before and after installation, and the soil was collected at more than three points in parallel with the highway at the gauze installation point. The components in gauze and soil were investigated in addition to the deicer components (Ca2+, Na+, Cl-) as well as Mg2+ and K+. As Ca2+ and Cl- components of deicer were affected by agricultural use, Na+ component was selected as a tracer and further SAR (Sodium Adsorption Ratio) of soil was analyzed to examine the degree of influence on vegetation indirectly. The gauze concentration was evaluated by the concentration of the deicer ingredient at the background concentration of the blank gauze. The total amount of the deicer sprayed in the study road for 4 months (winter season) was about 93 ton/km. In the gauze test, the spread of the deicer was detected at a distance of 100 m in study area, but the concentration of the deicer in the gauze by distance decreased rapidly within 10 m from the highway. And concentration of the deicer components in gauze and soil came down after rainy season (August ~ Sep.). The results showed that the components of the deicer could be spread widely by the wind. The effective range of the deicer on vegetation based on SAR in soil was estimated to be less than 20 m from the highway. This study examines the concentration changes of the deicer components in gauze and soils and shows that deicer components sprayed on the highway are accumulated and moved over time by wind, snow removal, terrain, water system and land use around the highway.

The micron-sized indium zinc tin oxide (IZTO) particles were prepared by spray pyrolysis from aqueous precursor solution for indium, zinc, and tin and organic additives such as citric acid (CA) and ethylene glycol (EG) were added to aqueous precursor solution for indium, zinc, and tin. The obtained IZTO particles prepared by spray pyrolysis from the aqueous solution without organic additives had spherical and filled morphologies, whereas the IZTO particles obtained with organic additives had more hollow and porous morphologies. The micron-sized IZTO particles with organic additives were changed fully to nano-sized IZTO particles, whereas the micron-sized IZTO particles without organic additives were not changed fully to nano-sized IZTO particle after post-treatment at 700 °C for 2 hours and wet-ball milling for 24 hours. Surface resistances of micron-sized IZTO’s before post-heat treatment and wet-ball milling were much higher than those of nano-sized IZTO’s after post-heat treatment and wet-ball milling. From IZTO with composition of 80 wt. % In2O3, 10 wt. % ZnO, and 10 wt. % SnO2 which showed a smallest surface resistance IZTO after post-heat treatment and wet-ball milling, thin films were deposited on glass substrates by pulsed DC magnetron sputtering, and the electrical and optical properties were investigated.

To identify subspecies and stocks of minke whale meats purchased from Korean markets during 2005-2007, we first obtained their complete sequences of mitochondrial DNA cytochrome b and control region sequences, and compared these sequences to the corresponding sequences of the common minke whale (Balaenoptera acutorostrata), obtained from GenBank. From analyses with partial cytochrome b sequences (383 bp) and non-coding, partial control region sequences (463 bp), Korean mink whale meats are identified as products from the North Pacific minke whale (B. a. scammoni). In addition, the sequences of the partial control region from these meats showed G at site no. 298 and G or A at site no. 463, and the meats appeared to originate from the J stock within this subspecies. Thus, because the J stock has been protected since 1986, implementation of strict regulation measures to reduce their accidental fisheries by catch seems urgent. In addition, B. a. scammoni is distinct from B. a. acutorostrata, with an average Jukes-Cantor distance of 2.21% in the complete control region sequence analysis (935 bp) and 1.31% in the complete cytochrome b gene sequence analysis; the current results support the current subspecies classification, although further sequencing analyses with nuclear genes are necessary.

Ooencyrtus nezarae Ishii and Gryon japonicum (Ashmead), egg parasitoids of Riptortus pedestris (Fabricius), coexist despite direct competition for host eggs. As asymmetrical pattern of seasonal occurrence, i.e., more G. japonicum during spring-summer and more O. nezarae during summer-fall, has been reported, host resource partitioning may occur in temporal scales. To test this hypothesis, we demonstrated the interspecific competition between the two species by measuring parasitism in nine combinations of host densities and exposure times. To reflect gregarious-solitary dichotomy, three O. nezarae and one G. japonicum mated females were used in each experiment. O. nezarae was better competitor when exposure time was longer than 1 day irrespective of host densities. Parasitism rate and progeny emergence of O. nezarae was 1.6-2.8 and 4.7-7.3 times higher than G. japonicum. O. nezarae has higher potential rate of increase than G. japonicum due to gregariousness, and be more successful in larval competition inside multiparasitized host egg as it acts as a facultative hyperparasitoid. Although G. japonicum was more effective in host finding (as they showed relatively higher per capita parasitism and progeny emergence), their progeny suffered high mortality from the larval competition with O. nezarae. These results may explain the asymmetrical occurrence pattern in the field.

Testes‐derived unipotent male germ‐line stem (GS) cells can acquire multipotency under appropriate culture conditions to become mGS cells which can contribute to all three germ‐layers. This study was designed to investigate the epigenetic characteristics of mGS cells derived from adult mouse testes (maGS cells). The GS cells were isolated from 4 6 week DBA mouse and were cultured in Dulbecco’s modified Eagle Medium supplemented with 15% (v/v) fetal bovine serum, 1,000 U/ml LIF, 4 ng/ml GDNF at 37℃ in an humidified atmosphere of 5% CO2 in air to derive the maGS cells. The multipotency of maGS cells were verified by morphological and gene expression analyses, teratoma formation upon transplantation into nude mouse and in vitro differentiation ability. Bisulfite genomic sequencing revealed that GS cells had androgenetic DNA methylation pattern at the Igf2‐H19, Gnas‐Nespas , and Dlk1‐Dio3 imprinted gene clusters which changed to hemi‐zygotic embryonic stem (ES)‐cell like pattern in the maGS cells. Western blot analysis, using modification‐ and residue‐specific antibodies, revealed that both maGS and ES cells had similar level of histone di‐methylation at 4th and 27th lysine residue of histone 3 (H3K4me2 and H3K27me2) which represent “bivalent domain” for regulating self‐renewal and differentiation of mouse ES cells. Both maGS and ES cells also shared similar hisone modification for H3K9me2, H3K79me2, H3K9ac and H3K18ac. However, maGS cells had higher level of H3K- 36me2 and H3S10p. These data suggest that maGS and ES cells share several epigenetic characteristics but they also have their own unique epigenetic marks that may be useful as a molecular marker for their identification.

Cryptic mealybug (CMB), Pseudococcus cryptus, is a serious scale pest on citrus, especially grown in plastic greenhouse on Jeju island, Korea. Binomial sampling plan was developed using the relationship between infested proportion of twig with less than T scales (pT) and mean density per twig (m) according to the empirical model ln(m)=α+βln(-ln(1-pT)). T was defined as tally threshold and set to 1, 2, 3, 4 and 5 scales per twig in this study. T=3 was the best tally threshold for estimating densities of CMB based on the precision of the model. Binomial model with T=3 provided reliable predictions of mean densities of CMB observed on commercial citrus orchards. The obtaining model was evaluated by resampling validation of Wald's sequential binomial plan and by fit at 95% confidence intervals for independent collected data sets. Binomial sampling program for decision-making of CMB population level was obtained at the action threshold 0.10, proportion of twig infested with more than 3 scales.

This study was conducted to determine the number of days required to break a plant’s dormancy and promote subsequent crop growth in new varieties of Gomchwi through the 4℃ treatment. Three new varieties of Gomchwi namely, ‘Sammany’, ‘Gommany’, and ‘Damogy’ were observed in this study. The rate of leaf emergence of ‘Sammany’ after 15-day of 4℃ treatment was 100%, while ‘Gommany’, and ‘Damogy’ took 20-days and 10-days, respectively to reach to 97.9% rate of leaf emergence. After 10-days of 4℃ treatment, ‘Damogy’ grew faster than the other varieties. and Harvest time for ‘Damogy’ was on January 18th, after 5-days of 4℃ treatment and yield was observed to be the highest at 15-days of 4℃ treatment. ‘Sammany’ was next with a minimum of 10-days of 4℃ treatment, although 15-days is more preferred for better harvest. ‘Gommany’ on the other hand, did not grow enough for harvest by January 18th, and its harvest time was delayed to January 31st. It needed a minimum of 15-days and preferentially 20-days of 4℃ treatment to grow normally and be ready for harvest. The plant height, leaf length and leaf petiole length appeared to grow better by extending duration of the 4℃ treatment. The number of leaves of ‘Sammany’ and ‘Gommany’ varieties was three leaves for the 5-days treatment which may be due to the incomplete breaking of dormancy. Regarding the yield per plant, ‘Sammany’ yielded 112.3 grams (g) in 15-days treatment, and ‘Gommany’ yielded 106.5 g in 25-days treatment. In the case of ‘Damogy’, it yielded 123.5 g and 183 g in the 10-days and 25-days treatment respectively. It is concluded that ‘Damogy’, ‘Sammany’ and ‘Gommany requires 10, 15, and 20 days of 4℃ treatment to break the plant’s dormancy and promote better plant growth.

Background : Despite the presence of various bioactive compounds in ginseng, there is lack of study on the variations of bioactive compounds in ginseng according to the cultivation of soil and the applied fertilizer types (or amount). Therefore, this study aims to examine the variations of 37 fatty acids (FA) and 8 vitamin E (Vit-E) vitamers in 6-year-old ginseng root cultivated in different soil types with different fertilizers regimes. Methods and Results : The profiling of 37 FAs and 8 Vit-E vitamers in 6-year-old ginseng roots was measured by gas chromatography coupled with a flame ionization detector, and then these results were statistically analyzed with chemometrics. The FA and Vit-E content in ginseng roots varied significantly with respect to soil cultivation conditions due to organic fertilizer types and amounts used. Unsaturated FA in ginseng is approximately 2.7 fold higher than the saturated FA. Linoleic, palmitic, and oleic acids were the most abundant FAs found in the ginseng roots. Also, the major Vit-E vitamer found in ginseng root is α -tocopherol. In particular, the application of rice straw compost or food waste fertilizer was increased to create nutritionally-desirable FAs and bioactive Vit-E in ginseng root. In addition, phytonutrient profiling coupled with chemometrics can be used to discriminate the cultivation conditions of ginseng. Conclusion : This preliminary study extends our understanding about the variations of FA and Vit-E in ginseng root depending on cultivation conditions. Hence, these results can be useful as basic information for reliable ginseng production containing high amounts of phytonutrients in a paddy-converted field.

Background : The effective components of Omija(Schisandra chinensis Bailllon) are lignans (schizandrins and gomisins), and this components were contented mostly in seed part on Omija, which have various physiological functionalities such as anti-cancer, anti-inflammatory, and antioxidant activities. Methods and Results : This study was carried out to determine effective condition(CO2, CO2+ethanol) on extraction using supercritical carbon dioxide extraction (SFE) system and to find interrelation on effective components and antioxidant activity of extracts and residues obtained after extraction. Effective components were analysed lignans and phenolic compounds and antioxidant activirty was determined for DPPH radical scavenging ability on methanol extracts of SFE-extract and SFE-residue. On SFE with ethanol, SFE extract was separated two phase, upper(water phase) and lower(oil phase). SFE-extract showed the highest total lignans content(61.36 mg/g, 72.14 mg/g on lower, 50.58 mg/g on upper) and the lowest total phenolic compounds(6.52 mg/100g) and SFE-residue showed the lowest total lignans content(1.45 mg/g) and the highest total phenolic compounds(16.23 mg/100g) by extracted on CO2+ethanol treatment. SFE-residue methanol extract showed the highest DPPH radical scavenging abilities and SFE-extract upper showed the lowest. Conclusion : Thus, this results showed SFE-extract showed the highest total lignans content, but SFE-residue showed the highest DPPH radical scavenging ability although the lowest total lignans content.

Background : This study was investigated the effect of organic fertilizer application and stem training methods on the growth and yield of Cynanchum willfordii. Methods and Results : Traditional species, C. willfordii conducted the test to the field in Chungbuk ARES, Korea. Two methods, non-supporting (custom) and I-shape supporting was used for stem training method. Planting spacing was adjusted to 30 (interrow spacing) x 20 cm (intrarow spacing) and other key management is the followed the cultivation manual for standard medicinal crops. Organic fertilizer application is conducted in mid-March before planting of C. willfordii. Registered organic agricultural materials such as fungal cultures (CC), mixed organic materials (MO), fermented and mixed expeller cake (EC), and fermented fowl dropping (FD) was used. Application rate is based on the nitrogen application rate after soil testing. Plant height was both good in all at I-shape supporting (266.7 ㎝) and all in non-supporting (160.8 ㎝); however, stem diameter was more thicker in custom (4.6 ㎜) of I-shape supporting and EC (4.8 mm) of non-supporting. Number of branched stem were good at EC from I-shape supporting and non-supporting, as well as leaf growth. SPAD value was evaluated in MO (52.7) of I-shape supporting and EC (52.3) of non-supporting. Number of branched root per organic fertilizer were showed 7.0 at MO from I-shape supporting, but 7.3 at FD from non-supporting. While length of main root were 27.0 cm in MO of I-shape supporting and 31.3 ㎝ in FD of non-supporting. Root weight also more heavier in EC (66.3 g) of I-shape supporting and FD (53.0 g) of non-supporting. When applied organic fertilizer, total density of soil microorganisms were changed into 38.4 ppm in custom; however, it more plentiful of 90.7 ppm in MO, it showed good effect on the replication of soil microbiota. Conclusion : From the investigated results, MO of I-shape supporting was good at overall growth, including good tendency of roots growth.

Background : The effect of ridge-up bed cultivation and stem training method on the plant growth and yield of Cynanachum willfordii was investigated. Methods and Results : Domestic variety of Cynanachum willfordii was tested at Chungbuk ARES under the following conditions. Nonsupporting (custom) and I-form supporting was used for stem training method, and ridge height was set as 30 (custom) and 60 cm (high ridge). Planting spacing was adjusted to 30 (interrow spacing) × 20 cm (intrarow spacing), and other major management was followed the method of standard cultivation for medicinal crop. Investigated result from leaf characteristics, leaf length was longer in high ridge cultivation (HRC) as 11.1 cm than custom cultivation (CC) as 10.6 cm. Leaf width is proved to be 12.8 (HRC) > 11.2 cm (CC). Leaf number is proved to be 294 (HRC) > 254/plant (CC), with higher number of 44/plant at HRC and weight/10 leaves were more heavy at HRC (4.9 g) than that of CC (2.6 g). It was more fruit setting at HRC over 15/plant. According to the stem training method and ridge height from nonsupporting cultivation, main root was 4.0 (CC), higher than that of HRC over 0.5/plant. However, root length was more longer in HRC (28.6) than that of CC (25.0 cm). Main root diameter was also showed more thicker pattern in HRC. From staking cultivation, root number of HRC was 7/plant, it was recorded more 3/plant than that of CC, and it was also same pattern in main root length and root diameter. By the standard of commercial root, yield of living roots in nonsupporting cultivation were 59.0g/plant (HRC), it was more heavy over 10.4g/plant than that of CC. In staking cultivation, HRC were recorded as 74.2g/plant, more heavy 6.9g/plant than that of CC. Yield from I-form support stem training and ridge-up bed cultivation of HRC was higher approxmiately 52.7% than that of CC. Conclusion : Overall growth by high ridge cultivation of I-form support was good and yield of HRC was also increased over 52.7% than that of CC.

Late blight caused by Phytophthora infestans is historically a serious epidemic disease in potato and tomato cultivations. Accession L3708 (Solanum pimpinellifolium), a new source for late blight resistance was identified in AVRDC, and carries the resistance gene, Ph-3, incompatible to P. infestans race 3. The AFLP markers linked to Ph-3 were previously developed from the L3708 accession (Chunwongse et al. 2002). To facilitate tomato breeding with the Ph-3 gene, an attempt was made to convert AFLP markers to sequence-characterized amplified region (SCAR) markers. Among 6 AFLP markers, only one AFLP marker, L87, was successfully converted to SCAR marker. The resistance-specific 230 bp AFLP fragment was cloned and sequenced, and the PCR primer amplifying a 123 bp fragment was designed. This SCAR marker could discriminate resistant and susceptible individuals with high stringency. The developed SCAR marker could be used for the marker assisted-selection in tomato breeding programs.