본 논문은 모바일 폰 사진을 활용하여 도시 공간에서 전력선과 건물 사이의 거리를 편리하게 모델링하고 측정하는 방법에 중점을 두고 있으며, 이에 대한 정확성을 평가하였다. 모바일 장 치용 2뷰 지오메트리를 사용한 전력선과 건물의 3D 재구성 방법을 설계하여 어디에서나 즉시 측정할 수 있도록 하였다. 특징점을 사용하여 전력선과 건물 사이의 거리를 계산하고 건물 정 보를 활용하여 게임 가상 공간에 전력선과 전주를 배치할 수 있다. 본 논문에서는 대전시 내 10개의 위치에서 실험을 실시하고, 상업용 시스템과 LiDAR가 있는 경우와 없는 경우를 포함 한 이전 방법과의 성능을 평가하였다. 우리의 방법을 스마트폰에 구현했기 때문에 장점은 휴 대성과 저렴함이다.

Subunit vaccines are being developed as a potential therapy for preventing microbial pathogen infection. In this study, the immunogenicity of recombinant Brucella (B.) abortus Fe/Mn superoxide dismutase (rFe/Mn SOD) protein as a subunit vaccine against B. abortus was investigated in BALB/c mice model. Brucella Fe/Mn SOD gene was cloned into a pcold-TF DNA vector. The bacterial recombinant protein was expressed using the Escherichia coli DH5α strain with a size of 82.50 kDa. The western blotting assay showed that rFe/Mn SOD reacted with Brucella-positive serum, indicating the potential immunoreactivity of this recombinant protein. After the second and third vaccinations, the peripheral CD4+ T cell population was increased significantly in the rFe/Mn SOD-immunized mice group compared to the PBS control group. Moreover, immunization of this recombinant protein increased the CD4+ T cell population from the first vaccination to the third vaccination. Meanwhile, the CD8+ T cells were slightly enhanced after the second vaccination compared to the first vaccination and compared to control groups. Fourteen days after the bacterial infection, the splenomegaly and the number of bacteria in the spleen were evaluated. The result showed that both rFe/Mn SOD and positive control RB51 decreased the bacterial replication in the spleen and the splenomegaly compared to control groups. Altogether, these results suggested that rFe/Mn SOD could induce host immunity against B. abortus infection.

This study aims to investigate the effects of exogenous succinic acid (SCA) on Brucella (B.) abortus infection in macrophage RAW 264.7 cells and ICR mice. Firstly, the in vitro experiment was conducted by MTT cytotoxicity and bacterial internalization assay to evaluate the uptake of B. abortus into macrophage cells. Two non-cytotoxic concentrations of SCA demonstrated attenuated invasion of Brucella into macrophages at 30 and 45 min post- infection (pi). Secondly, ICR mice were treated with SCA and infected with B. abortus. On day-14 pi, spleen and blood serum were collected to evaluate the bacterial burden and total spleen weight as well as the production of cytokine/chemokine, respectively. The results showed that SCA treatment promoted bacterial growth and reduced the total spleen weight in mice. Furthermore, SCA treatment increased the level of IL-10 cytokine in the sera, while dampening the production of MCP-1 chemokine compared to the control. The results of bacterial load in spleen and spleen weight together with cytokine/chemokine production profile in the sera indicated that SCA induced the host anti-inflammatory response which is beneficial for the survival of Brucella. Therefore, these findings suggest that SCA contributed to host immunity against Brucella infection and the emerging potential topic-immunometabolism should be invested for further investigations.

This study investigated the efficacy of four Brucella (B.) abortus recombinant proteins, namely adenylate kinase (Adk), nucleoside diphosphate kinase (Ndk), 50S ribosomal protein (L7/L12) and preprotein translocase subunit (SecB), as a combined subunit vaccine (CSV) against B. abortus infection in BALB/c mice. Immunoblotting assay showed that these four recombinant proteins as well as pcold-TF vector reacted individually with Brucella-positive serum, but not with Brucella-negative serum. The peripheral blood CD4+ T cell population was increased in CSV-immunized mice compared to PBS and pcold-TF vector groups. In addition, CSV and pcold-TF groups displayed induced IgG1 and IgG2a antibodies production compared to PBS and RB51 group, whereas IgG2a titer was higher than IgG1 titer in CSV group. The secretion profiles of IgG1 and IgG2a production together with an enhancement of CD4+ T cell population suggested that CSV did not only induce T helper 1 (Th1) T cell immunity but also humoral immunity. Therein, Th1 T cell immunity is more predominant in eliminating intracellular bacteria B. abortus. Furthermore, CSV immunization significantly reduced the bacterial burden in the spleen as well as the spleen weight in comparison to PBS and pcold-TF groups. Altogether, combination of these antigens could be potential to induce protective immunity against B. abortus infection in animals.

This study was conducted to investigate effect of feeding beet pulp on reproductive performance, colostrum composition and microbiological characteristics in sows. A total of 12 sows(Landrace×Yorkshire) were randomly allotted three dietary treatments at gestating 90 days. The diets were prepared by adding 0, 5 and 10% of the beet pulp in basal diets. The pigs fed 5% beet pulp showed higher litter size than the 0 and 10% beet pulp treatments, although there was not significant among the treatments. Similarly, birth and 21days weight in piglet were not different among the treatments. In colostrum fat, protein, lactose, citric acid, solid, ffa, true protein were ranged to reference values, but not difference among the treatments. E.coli of feces was lower(P<0.01) in the 5% beet pulp than the 0 and 10% beet pulp treatments. But no effect was observed on Lactobacilli of feces among dietary treatments. These our results indicate the beet pulp has no significant effect of reproductive performance and colostrum composition in sows. But, 5% level beet pulp is effective for reduction of intestinal pathogenic microorganisms.

The objective of this study was to evaluate the effect of the Allomyrina dichotoma larva (AD) on allergy and inflammation.We examined inhibitory effect of AD on allergic reactions in mast cells (RBL-2H3) activated by Compound 48 / 80and inflammatory response in macrophages (Raw 264.7) activated by LPS. Anti-allergy and anti-inflammatory actions ofAD water extract had no cytotoxicity. At these concentrations AD inhibited ẞ-hexosaminidase, tumor necrosis factor- α(TNF- α), interleukin-4 (IL-4), interleukin-6(IL-6) and cyclooxygenase-2(COX-2). AD also inhibited the expression of inducibleNO synthase (iNOS). AD reduced the release of inflammatory cytokines including IL-4, IL-6, TNF-α, and ẞ-hexosaminidase.These results suggest that AD may be potential anti-allergy and anti-inflammatory therapeutic agent.

The occurrence pattern of lepidopteran adults in a paddy-upland rotation field and an organic upland field for 8 maize and 7 sorghum varieties was surveyed from May 8 to September 27, 2016. In addition, their damage ratio and the invasive pores by Ostrinia furnacalis larvae as well as harvest yields were monitored. The density of the lepidopteran adults in two fields showed similar pattern, although a little different pattern in Heliothis assulta. The damage ratio of maize and sorghum varieties by O. furnacalis at harvest periods was depended on both varieties and cultivated environments. A middle negative linear correlation was observed in damage ratios vs yields, the number of invasive pores vs yields, and the number of larvae in corn ears vs yields, but there was not a specific correlation between the damage ratio vs the number of invasive pores. These results may be useful for the establishment of a management strategy to control oriental corn borer in paddy-upland rotation or organic upland fields for maize and sorghum.

The purpose of this study was conducted in order to analyze the effects of the manual intervention and self-corrective exercise models of general coordinative manipulation(GCM) on the balance restoration of spine & extremities joints with distortions and mal-alignment areas. The subjects were the members who visited GCM Musculoskeletal Prevent Exercise Center from March 1 2012 to December 31 2013 because of spine & extremities joints distortion and mal-alignments, poor posture, and body type correction. All subjects were diagnosed with the four types of the GBT diagnosis. And according to the standards of the mobility vs stability types of the upper & lower body, they were classified into Group 1(40 persons) and Group 2(24 persons). For every other day for three times a week, GCM intervention models were applied to all subjects for four weeks, adding up to 12 times in total. Then the balance restoration effects were re-evaluated with the same methods. The results are as follows. 1) Balance restoration effects of VASdp(Visual analysis scale pain & discomfort) and ER(Equilibrium reaction: ER) came out higher in GCM body type(GBT) Ⅱ·Ⅲ·Ⅳ of Group 1. 2) In case of balance restoration effects in Moire and postural evaluation areas, Group 1 was higher and cervical and scapular girdle were higher in Group 2. The balance restoration of the four GBT types was significant in all regions(p<.05), and the scapular girdle came out as high in the order of GBTⅡ·Ⅳ·Ⅰ. 3) In case of thoracic-lumbar scoliosis and head rotation·facial asymmetric· cervical scoliosis·ribcage forward, the balance restoration effects of the upper body postural evaluation areas came out the highest in Group 1 and Group 2, respectively. The balance restoration effects of the four GBT types were significant in all regions(p<.05), and came out the highest in lumbar scoliosis GBTⅢ·Ⅰ, ribcage forward and thoracic scoliosis GBTⅡ·Ⅳ. 4) The balance restoration effects of the lower body postural evaluation areas came out higher in Group 1 and Group 2 for pelvis girdle deviation·patella high·umbilicus tilt and hallux valgus·foot longitudinal arch: FLA·patella direction, respectively. The balance restoration effects of the four GBT types were significant in all regions(p<.05), and came out the highest in pelvis girdle deviation GBTⅢ·Ⅰand patella high-direction GBTⅣ·Ⅱ·Ⅰ. 5) The balance restoration effects between the same GBT came out significant (p<.05) in all evaluation areas and items. The conclusions of this study was the manual intervention and self-corrective exercise models of the GCM about the mal-alignment of the spine & extremities joints across the whole body indicated high balance restoration effects(p<.05) in spine & extremities joints in all evaluation areas.

Experiments were conducted in order to assess the healing effect of bee venom (BV) cream on full-thickness skin wounds in rabbits. BV cream was compared with silver sulfadiazine (SS) as a topical medicament against a control on experimentally created full-thickness wounds. Two wounds measuring 2 × 2 cm were created bilaterally (four wounds/rabbit) on the dorsolateral aspect of the trunk of seven New Zealand white rabbits. Wound treatments were evenly distributed on four sites, using a Latin square design. The contact layer of wounds was treated with physiological saline (control), SS cream, and BV cream over a period of 28 days. Assessment of wound healing was based on scab hardness, wound exudates, wound area, unepithelialized granulation tissue, and histopathological findings. Topical application of BV and SS creams to wounds resulted in reduced inflammation, debridement of necrotic tissue, and promoted granulation and epithelialization. Wound healing was faster, with statistical significance in BV and SS treatments, compared to the control (P<0.05). Treatment with BV evoked an anti-inflammation effect in a rabbit model. BV cream produced a wound healing effect similar to that of commercially available SS cream. Anti-inflammation effect as a topical treatment with BV cream appears to be better than that with SS cream. These results suggest that topical application of BV cream may be an alternative treatment for full-thickness skin wounds.

The objective of this study was to determine the effects of E. coli isolated from porcine semen on sperm viability, motility, and semen pH. Semen samples were prepared using commercial extender, SeminarkPro (Noahbio Tech, Korea) that did not contain antibiotics. And 4 different levels of E. coli were artificially innoculated to semen with following concentrations; 4,000 of sperms with 1 of E. coli (T1), 400 with 1 (T2), 40 with 1 (T3), and 4 with 1 (T4). Semen samples were preserved at 17℃ for 5 days in semen storage box until analyzed by flowcytometer. Aliquots were subjected to measure the sperm viability (Live/Dead® stain), motility (mitochondrial function), and semen acidity (pH) from day 0 (day of semen collection) to day 5. Sperm motility and viability were significantly decreased (p<0.05) on day 0 (4 hrs after preservation at 17℃) in T3 and T4 compared to control groups and were significantly decreased (p<0.05) in all groups from day 3. Sample pH was acidic in T3 (6.90～6.86) and T4 (6.86～6.65) from day 3 to day 5 (p<0.05). On the other hand, sample pH was maintained 7.0～7.1 in control, T1, and T2 during the experimental period. Sperm motility and viability were significantly decreased from day 0 to day 5 compared to control in samples contaminated with E. coli above a value of 40:1 (20×106 sperm cells/ml : 5×105 cfu/ml). Even on day 1 in T4 and on day 3 in T3, semen pH was acidic probably due to the acidification of dead spermatozoa. These results suggest that E. coli contamination has a concentration-dependent detrimental effect on extended porcine semen quality.

This study was designed to analyze repetitive dorsiflexion and plantarflexion exercises in ankles have effects on the muscle tones of triceps, vastus medialis and gastrocnemius. 25 healthy woman volunteers(ankle dorsiflexion exercise group: 13, ankle plantarflexion exercise group: 12) participated in the two ankle exercise models, which have performed 3 times a week for 3 weeks. Myotonometer was used to measure the muscle tones of the triceps, vastus medialis, gastrocnemius muscles, and measurements were performed before and after the application of the each exercise model. Repeated measured paired t-test and independent t-test was used to determine a statistical significance. The group of repetitive dorsiflexion exercise of the ankle have effects on the muscle tones of triceps, vastus medialis and gastrocnemius (p>.05). The group of repetitive plantarflexion exercise of the ankle have effects on the muscle tones of triceps, vastus medialis and gastrocnemius(p>.05). The groups of repetitive dorsiflexion and plantarflexion exercise of the ankle have effects on the muscle tones of triceps, vastus medialis and gastrocnemius(p>.05).

The leaf beetle, Chrysolina aurichalcea (Coleoptera: Chysomelidae), is a pest damaging plants of Compositae. In order to understand the genetic diversity and geographic variation of the species we sequenced a portion of mitochondrial COI gene (658 bp) and complete nuclear internal transcribed spacer 2 (ITS2) collected from seven Korean localities. A total of 18 haplotypes (BARCA01 ~ BARCA18), with the maximum sequence divergence of 3.04% (20 bp) were obtained from COI gene sequence, whereas 17 sequence types (ITS2CA01 ~ ITS2CA17), with the maximum sequence divergence of 2.013% (9 bp) were obtained from ITS2, indicating substantially larger sequence divergence in mitochondrial gene sequence. Phylogenetically, the mitochondrial DNA has shown several haplotypes formed independent groups with substantially high node support (≥ 90%), whereas no such grouping was evidenced for ITS2, indicating different behaviors of the two molecules. Such difference may reflect a diverse dynamics of the species such as biogeographic history, mating behaviors, and also possibly different mode of inheritance of the two molecules, but requires further scrutinized examination of the dataset. In terms of population genetic perspective, overall no population subdivision was detected from both molecules, except for locality 7 (Eocheong islet) from mitochondrial DNA. As more scrutinized analysis is performed, further fruitful inference on the geographic contour of the species might be available.

A nine-month-old male Pekingese weighing 5.7 kg was admitted to the Veterinary Medical Center at Chungbuk National University with a history of acute nonambulatory tetraparesis after minor trauma. A diagnosis of atlantoaxial instability with a dens axis fracture was based on examination of survey spinal radiographs and was confirmed during surgery. A modified ventral fixation technique using cortical screws was used for stabilization of the atlantoaxial joint. Serial evaluation based on radiographic and neurologic assessment was performed eight weeks after surgery. Symptoms of tetraparesis disappeared gradually, and arthrodesis of the atlantoaxial joint using a ventral fixation technique has maintained stable fixation.

A new species, Hatchiana n. sp., is confirmed by morphological and molecular data. The genus Hatchiana Fender, 1966 belonging to Podabrini LeConte, 1881 is distinguished by the shape of tarsal claws: all tarsal claws with blunt tooth in both sexes. The genus was recognized as a subgenus of Podabrus Westwood, 1838. Recently, however, Hatchiana was suggested as separated genus. The genus Hatchiana was known as 12 species in Palaeartic region including three species from Korea. In this study, we found Hatchiana n. sp. from several areas of Taean-gun, Chungcheongnam-do, Korea. This new species is different from closely relative species, Hatchiana jirisanensis (Kang & Kim, 2000) by following morphological characters: size of compound eye, length of antennae, shape of pronotum, shape of scutellum, and shape of aedeagus. Also, we compare the DNA barcoding region (the former region of CO1 gene) between these two species as molecular characters. In result, Hatchiana n. sp. is distinct from Hatchiana jirisanensis Kang & Kim by discrepancy of three percents in CO1 sequence. Therefore, the Korean Hatchiana is confirmed as four species, adding Hatchiana n. sp.

Al though the changes in tooth morphology and hardness by hydrogen peroxide(H20 z) have been r‘epor‘.ted .‘ the pαr。o야t뻐ec야tive role of heme oxygenase-l(HO-l) against the cytotoxic effects of H202 has not been clarifïed i n human pulp cells ln this st udy. we investigated whether HO-l is involved in Hz0 2-induced cytoLox icity a nd examined the production 0 1' dentin sia lophosphoprotein(DSPP) and other mineralization markers‘ in hllman pu lp cells H202 decreased cell viabi lity, but increased HO-l and DSPP expression in a concentra tion and time dependent manner . Inhibitors of gllanylate cyclase. PI3K, ERK. and p38 MAP kinase blocked H202-indllced cytotoxicity and the expression of HO-l and DSPP mRNAs in pulp cells. These data suggest that the induction of HO-l by H202 in plllp cells plays a protective role against the cytotoxic effects 0 1' HzOz and stimulates DSPP expression‘ reslllting in prematllre odontoblast dilTerentiation throllgh pathways that involve cGMP‘ p38. and ERK.

Al t hough substance P(SP) , a potent pro- inflammatory peptide, is involved in inflammation and immune responses‘ t he eff'ect of SP on t he expression of macrophage inflammatory protein 3a (MIP- 3α CCL20) in periodontal liga ment(PDL) cell s a re unknown, Equally as enigmatic is the link between SP, t he stress protein heme oxygenase- l(HO-l) ‘ and CCL20 procluction, We investigated whether SP induces the release of chemokine CCL20 from immortal ized PDL(IPDL) ceJJ s‘ and fur ther c l a꺼 SP mediated pathways, We also examined the relationship between HO-l a ncl CCL20 by t reating PDL cells with SP, Incubating IPDL cells with SP increased expression of CCL20 mRNA a nd CCL20 protein in a dose-time dependent manner Highly selective p38 and ERKl/2 inhibitors abrogated SP-induced expression of CCL20 in IPDL cell s, SP is a lso responsible for ini t iating phosphorylation of I/C B, degradation of Iκ B‘ ancl activat ion of NF'-/C B, SP induced expression of HO-l in both a concentration- and time-dependent man nel ‘ and CCL20 refl ected s imilar patterns, The inductive effects o[ SP on HO- l and CCL20 wer e enhanced by HO- j inducer hemin and the membrane-permeable cGMP analog 8-bromo-cGMP, Conversely, this pathway was inJübited by t he 1-10난 inhi bitor zinc protoporphyrin IX(ZnPP IX) and the selective inl뼈itor of guanylate cyc1ase‘ lH-[l , 2, 4Joxad iazole[4‘ 3-aJquinoxal in-l-one (ODQ) , We report herein the pathway that connects SP along with other modulators 。f neuroimmunoregulationto the induction of HO-l and t he inflammatory mediator MIP-3a /CCL20 in IPDL cell s‘ which play an important role in the development 01' periodontitis or inflamrnation during orthodontic tooth movem