In this study, the safety and efficacy of an spot on formulation composed of Fluralaner (40mg/kg)and Moxidectin (2mg/kg) against internal and external parasites was evaluated in Korean cats. The product was developed as a topical use in cats to prevent or control internal and external parasites including ticks, fleas, gastrointestinal parasites, ear mite and heartworm. As a result of the study, targeted for total 30 cats from 8 Korean animal hospitals, this combination of Fluralaner and Moxidectin was found to be very safe in cats, and its effectiveness against external parasites (ticks, fleas, and ear mites) was fully verified. In addition, As the animals exposed in the infective circumstances of heartworm and gastrointestinal parasites were remained negative during the study period of 12 weeks after treatment of test drug, it was determined that this product had a potential preventive effect against the heartworms and gastrointestinal parasites.

Lumpy Skin Disease (LSD) and Foot-and-Mouth Disease (FMD) cause substantial economic losses on the livestock industry. Therefore, vaccinations have been implemented as the control strategy in endemic countries. However, the potential adverse effects of administering vaccines for both diseases simultaneously have not been thoroughly evaluated. The aim of this study was to assess the impact of vaccinating dairy cows with either or both LSD and FMD vaccines on milk production and physiological parameters such as milk temperature, rumination time and body weight. The experimental groups were divided into four according to the injection materials: 1) saline, 2) LSD vaccine, 3) FMD vaccine, and 4) both vaccines. The impact of vaccination on milk yield and physiological parameters was evaluated daily until 12 days post-vaccination, and milk components were analyzed twice, once per week. Among the experimental groups as well as each vaccine group, no statistically significant differences (p < 0.05) were observed at milk yield, milk components, or milk temperature. This suggests that simultaneous vaccination of LSD and FMD can be administered without adverse effects.

본 연구는 절화 국화의 수경재배와 토경관비재배 시 생육 특 성을 비교하기 위하여 실시하였다. 그 결과 초장, 절화의 생체중 과 건물중은 토경관비재배에서 증가하였다. 식물체 내 전질소, 칼륨 및 칼슘의 함량도 토경관비재배에서 더 높았다. 반면에 줄기 직경은 수경재배에서 3.7mm로 증가하였다. 또한 개화소 요일수도 토경관비재배보다 수경재배에서 3일 정도 단축되었 고, 줄기 1개당 꽃 수도 19.6개로 수경재배에서 더 많았다. 결과 적으로 토경관비재배에서 식물체 내 무기염류 함량이 높고, 초장이 길어지고 생체중, 건물중이 증가하였지만 수경재배보다 개화기는 지연되었다. 또한 토경관비재배보다 수경재배에서 줄 기 직경이나 꽃 수 등이 증가하였다. 따라서 재배자의 입장에서 국화 수경재배를 통해 양분과 수분 공급량의 조절에 의한 개화 기의 조절, 조기 개화 및 절화 품질 향상 등의 효과를 기대할 수 있을 것으로 판단된다.

Habenaria radiata, commonly known as the Egret Flower, is a critically endangered terrestrial orchid facing rapid population decline due to overharvesting in Korea. Asymbiotic seed propagation offers a promising conservation method for this rare species. This study investigates the effects of different nutrient media on the germination and plantlet development of immature seeds of H. radiata, with the goal of establishing optimal propagation conditions. Among four media types, with the highest germination rate (75.6%) observed on OBTSG medium, followed by 1/2 MS, OMM, and OSGM. Protocorm development varied across media, with OBTSG and OMM supporting the most robust growth. A two-step culture method involving OBTSG for germination followed by OMM for plantlet development proved most effective, achieving an 84.4% survival rate. These findings highlight the importance of media composition and transitioning for successful propagation. The results provide a practical protocol for in vitro propagation, contributing to both in situ and ex situ conservation efforts aimed at preserving H. radiata and other endangered terrestrial orchids.

Seeds of some barberry species have embryo with physiological dormancy that requires a cold stratification for germination. Berberis amurensis Rupr. is a native species of Japan, Korea, the Russian far east, and parts of China. This specific plant is important for its edible fruits and rhizomes with high medicine value. This study aimed to determine the effect of stratification on germination and physiological change of B. amurensis. Seeds were placed on sterilized sand medium moistened with distilled water in 9 cm diameter petri dishes and stored at 4 and 25˚C for 0, 15, 30, 45 and 60 days. Each treatment had 40 seeds per replica, and three repetitions per treatment. Immediately after stratification, total phenolics contents (TPC) was analyzed and seeds incubated at 15/6˚C for 12 weeks. Warm stratification had a significant effect on seed forcing for germination than cold stratification treatment. At 25˚C for 60 days, stratified seed showed highest germination percentage (25.7±4.3%) and germination started in 14 days of the stored period. Whereas TPC was significantly decreased with increasing stored period. Contrastively, cold stratification had no effect on the germination ability. In the same way germination percentage of non-stratified seeds were also zero. The results confirmed that B. amurensis seeds were in a dormant state and warm stratification increased the germination ability by breaking of dormancy.

Mesocrystals are macroscopic structures formed by the assembly of nanoparticles that possess distinct surface structures and collective properties when compared to traditional crystalline materials. Various growth mechanisms and their unique features have promise as material design tools for diverse potential applications. This paper presents a straightforward method for metal–organic coordination-based mesocrystals using nickel ions and terephthalic acid. The coordinative compound between Ni2+ and terephthalic acid drives the particle-mediated growth mechanism, resulting in the mesocrystal formation through a mesoscale assembly. Subsequent carbonization converts mesocrystals to multidirectional interconnected graphite nanospheres along the macroscopic framework while preserving the original structure of the Ni-terephthalic acid mesocrystal. Comprehensive investigations demonstrate that multi-oriented edge sites and high crystallinity with larger interlayer spacing facilitate lithium ion transport and continuous intercalation. The resulting graphitic superparticle electrodes show superior rate capability (128.6 mAh g− 1 at 5 A g− 1) and stable cycle stability (0.052% of capacity decay per cycle), certifying it as an advanced anode material for lithium-ion batteries.

In order to predict the process window of laser powder bed fusion (LPBF) for printing metallic components, the calculation of volumetric energy density (VED) has been widely calculated for controlling process parameters. However, because it is assumed that the process parameters contribute equally to heat input, the VED still has limitation for predicting the process window of LPBF-processed materials. In this study, an explainable machine learning (xML) approach was adopted to predict and understand the contribution of each process parameter to defect evolution in Ti alloys in the LPBF process. Various ML models were trained, and the Shapley additive explanation method was adopted to quantify the importance of each process parameter. This study can offer effective guidelines for fine-tuning process parameters to fabricate high-quality products using LPBF.

GN01 is a new antiviral medicine acting against Korean Sacbrood virus (KSBV) of honeybees. It contains 5 mg/mL of active ingredient, double stranded RNAs(dsRNA), that homologous to KSBV ribonucleic acid coding coat protein (VP1) of virus and inducing RNA interference (RNAi). RNA medicine is generally recognized as safe for rapid breakage by intrinsic ribonuclease and limited absorption from gastrointestinal tract. However, there were no data of repeat-dose toxicity in laboratory animals for dsRNA targeting SBV. This study was performed to investigate toxicity of GN01 in SD rats after weekly oral dosing for 28 days and to determine its no-observed-adverse-effect-level (NOAEL). Male and female SD rats were orally administered with GN01 at 0, 25, 50 and 100 mg/kg bw/day of dsRNA once per week for 28 days (total 5 administrations). The highest dose 100 mg/kg bw/day was determined based on the maximum volume injectable (20 mL/kg bw) via gavage. During treatment period, clinical signs, functional and sensory responses, body weights, food and water consumption, ophthalmological findings and urinalysis were investigated. After treatment period, hematological and clinical biochemistry tests and examination of necropsy findings, organ weights and histopathological lesions were performed. There were no significant differences between all test groups and vehicle control group in all measured parameters. Therefore, the NOAEL of GN01 was determined 100 mg/kg bw/day, the highest dose administered. In conclusion, repeated oral administration of GN01, a dsRNA medicinal product, is safe even at the maximum available dose in rats.

한국의 정원수는 2015년 7개에서 2022년 98개로 급격히 증가하였다. 늘어나는 정원 인프라의 수에 비해서 유지관리를 위한 지속적 정책과 연구는 미진한 상황이다. 본 연구는 효율 적인 정원 관리 정책을 도출하기 위해 주민들의 인식을 조사 하였다. 토픽 모델링, 감성 분석 등 텍스트 마이닝을 활용해 정원 참여 유형에 따른 주민 인식을 조사하였다. 주민 주도 정 원 그룹에서는 ‘정원정책’, 관 주도 정원 그룹에서는 ‘주민에 게 미치는 영향’이라는 주제에 의견이 집중되었다. 감성 분석 에서는 주민 주도 정원 그룹이 관 주도 정원 그룹보다 긍정어 점수가 더 높았다. 이러한 결과는 주민과 만족도 사이에 강한 사회적 연결이 있음을 나타낸다. 또한 주민 주도 정원 그룹에 서는 유지관리 업무를 맡은 주민들이 이를 자기계발의 한 형 태로 여기는 것으로 나타났다. 이러한 결과는 주민 주도 정원 관리 유형이 정원정책에 시사하는 바가 크며, 정원 내 일자리 와도 관련이 있음을 알 수 있다.

As various accidents have occurred in underground spaces, we aim to improve the quality validation standards and methods as specified in the Regulations on Producing Integrated Map of Underground Spaces devised by the Ministry of Land, Infrastructure and Transport of the Republic of Korea for a high-quality integrated map of underground spaces. Specifically, we propose measures to improve the quality assurance of pipeline-type underground facilities, the so-called life lines given their importance for citizens’ daily activities and their highest risk of accident among the 16 types of underground facilities. After implementing quality validation software based on the developed quality validation standards, the adequacy of the validation standards was demonstrated by testing using data from two-dimensional water supply facilities in some areas of Busan, Korea. This paper has great significance in that it has laid the foundation for reducing the time and manpower required for data quality inspection and improving data quality reliability by improving current quality validation standards and developing technologies that can automatically extract errors through software.

To functionally classify the types of gardens that can be used in a city, this study examined the perceived functions of gardens in Korea by reviewing the previous case studies, field surveys concerning gardens in cities, and interviews with stakeholders. First, the roles and effects of gardens identified in the earlier studies were clustered into similar concepts. To ensure validity, the types of functions of the gardens were named based on opinions gathered through meetings with experts. Field surveys were conducted on 31 cases studied over four years. The data obtained were applied to supplement the redefined classification criteria and items for gardens in the city as described above. The current status of the gardens was analyzed by type. The gardens were divided into four categories: socio-cultural, environmental, economic, and health-related. The sociocultural type was dominant among the community gardens in Korea. The results of this study may be used as a reference for the intended function of building an urban garden and to prepare guidelines for introducing socio-cultural, environmental, economic, and health-type gardens in the future.

부추속은 수선화과에 속하며 약 1000여종에 이른다. 좀부 추는 자생 부추속에 속하며 식용이 가능하고 크기가 작아 분 화식물로 적합하다. 이에 따라 본 연구는 좀부추를 분화 소재 로서 개발하기 위하여 식물의 측지성장을 촉진하고 꽃의 품질 을 향상시키기 위해 수행되었다. 이를 위하여 좀부추의 구근 을 생장조절제(GA3, BA, 에테폰, TDZ)에 다른 시간(1, 5시 간)동안 침지처리했을 때 식물의 생장과 꽃에 어떤 영향을 주 는지 조사하였다. GA3 처리구는 엽수와 자구의 수가 증가해 증식을 촉진시키는 효과가 있었다. 게다가 GA3는 화수의 증 가와 조기개화를 유도했다. 그러나 엽폭과 화경의 폭은 감소 하였다. 에테폰은 좀부추의 초장과 초폭에서 영향을 주지 않 았으나 1h에서 자구의 수가 증가하였다. 에테폰은 화서 발생 을 억제하여 화경의 수가 감소하였다. BA는 식물의 생장과 개 화 특성에 유의적인 영향을 보이지 않았다. TDZ처리한 식물 은 왜화되고 생육상태가 불량하였으며, 식물을 고사시켰다.

Some consumer goods containing radioactive substances are in circulation and used in everyday life. In accordance with the Nuclear Safety Act, consumer goods with radioactivity are regulated. However, since most consumer goods distributed in Korea have no information that can confirm the amount of radiation, it is necessary to analyze the radiation for safety regulation. Among these consumer goods, GTLS (Gaseous Tritium Light Source) contains gaseous tritium (tritium, written as 3H or T), which is a radioactive material. The gaseous composition ratio in GTLS was analyzed using a precision gas mass spectrometer (Thermo Fisher, model MAT 271). As a result of GTLS analysis, the H2, HD or H3 +(T) or 3He, HT or D2 or He, DT, and T2, which correspond to the mass-to-charge ratio (m/z) 2 to 6 and the air components were detected. In addition, substances corresponding to m/z=24 and m/z=21 were also detected. These were compared with pure CH4 and those fragmentation patterns. The ratios of CT4 (m/z = 24) to CT3 (m/z = 21) and CH4 (m/z = 16) to CH3 (m/z = 15) were compared and they agree within the measurement uncertainty. We also performed additional experiments to separate the water component in the GTLS samples, considering the possibility that the m/z = 21 to m/z = 24 region is tritium compounds based on H2O. Despite the removal of the water components, peaks were detected at m/z=21 and m/z=24. Therefore, we confirmed that the component of m/z = 24 in the GTLS sample was CT4.