팽이버섯 포장필름의 국산화 및 동남아시아 지역을 대 상으로 한 수출용 필름을 개발하기 위하여, 개발된 포장 필름을 현재 시중에 유통 중인 포장필름 및 수입산 포장 필름과 함께 저장 중 팽이버섯의 저장 온도별 품질변화에 이어 유리당, 유리아미노산, 구성아미노산 등에 대한 영양 학적 특성변화를 검토하였다. 유리당 함량을 분석한 결과, 저장 온도가 높아질수록 유리당 함량이 약간씩 증가하는 경향을 보였으나, 포장 필름의 종류에 따라서는 큰 차이 를 나타내지 않았다. 유리아미노산의 경우 저장온도가 올 라감에 따라 methionine은 증가하는 반면, tyrosine은 감 소하였다. Polyphenol oxidase의 활성 확인결과 본 과제 로 인해 개발된 포장지가 우수함을 확인하였다. 베타글루 칸 함량은 건강 기능성 식품의 표준 지표로 사용되는 성 분이나 버섯 저장성을 판단하는 지표로 사용하기에는 적 당하지 않을 것으로 여겨진다.

팽이버섯 포장필름의 국산화 및 동남아시아 지역 수출용 필름개발을 목적으로 개발중인 포장필름을 현재 시중에 유통 중인 포장필름과 수입산 포장필름과 함께 저장 중 팽이버섯에 적용하여 이화학적 특성변화를 검토하였다. 각 포장재 원단으로 포장된 팽이버섯을 저장 온도에 따라 중량 감소율을 측정하여 선도유지를 평가하였다. 4oC에 보관한 팽이버섯은 모든 포장필름에서 중량 변화가 거의 없었으며 15oC의 경우 수입 포장필름과 국내 유통중인 포장필름이 시험용 포장필름 보다 더 많은 중량 감소율이 보이는 것을 확인하였다. 신장률을 측정한 결과, 15oC에서는 수입 포장필름, 국내 유통 중인 포장필름, 실험용 포장필름 순으로 신장률 변화가 크게 측정되었으나, 나머지 온도조건에서는 세 가지 포장 조건 모두 자루 신장률 변화가 비슷하게 나타났다. pH의 변화는 25oC에서 수입포장필름, 국내유통 포장필름, 실험용 포장필름 순으로 pH가 낮게 나타났다. 색도의 경우 저장 온도 4~25oC에서 저장한 시료는 저장 기간 동안 명도가 약간 낮아졌으나 포장 필름의 종류는 팽이버섯 색도 변화에 큰 영향을 미치지 않았다. 총 6종의 유기산이 검출되었으며, 종류는 oxalic acid(수산), lactic acid(젖산), malic acid(사과산), citric acid(구연산), acetic acid(초산) 및 succinic acid(호박산)로 나타났다. 팽이버섯의 유기산 총 함량은 저장 전 5.58%, 4oC에서 저장 후 4.70~5.50%, 15oC에서 저장 후 4.51~5.48%, 25oC에서 저장 후 4.91~6.07%, 35oC에서 저장 후 4.96~8.34%로 나타났다. 팽이버섯의 주요 유기산은 oxalic acid(1.11~4.28%)와 malic acid(0.48~1.96%)로 나타났다. 저장 온도에 따라서는 4~25oC에서 저장한 시험구는 유기산 함량의 변화가 크지 않았으나, 35oC에서 저장한 시험구는 malic acid의 함량이 낮아짐과 동시에 acetic acid의 함량이 높아졌다. 각각의 온도 조건에서 저장성을 실험한 결과 포장용 필름 원단에 따라서는 유기산 함량 변화에 큰 영향을 미치지 않는 것으로 나타났다.

Many wild mushrooms were collected at MT. Sambi areas for 1 day in July 2011. and 1 day in August 2011. They were identified and examined with references. According to the results, Species diversities are 1 division, 2 classes, 2 subclasses, 3 orders, 12 families, 18 genera and 24 species. Dominant families are Tricholomataceae. Resources of Wild Mushrooms were 4 species in edibility, 4 species in culture, 6 species in toxine, 5 species in medicine, 3 species in anticancer, 10 species in ectomycorrizahe and 12 species in rotten wood.

Schizophyllum commune belongs to Schizophyllum, Schizophyllaceae, Agaricales, Agaricomycetes and Basidiomycotatina. The morphological shape of S. commune was similar with the cap, fan and hair. As for the sensory evaluation, chewness was not good for human's sensory. The major useful component was identified schizophyllan from S. commune extract. Schizophyllan has a great potential for developing medical supplies and functional foods. In our study, we carried out to internal transcribed spacer regions(ITS) for the specimens systematically. Also, we found out the optimal growth conditions of different temperature and media for S. commune cultivation. As the results of these test showed that when we compare with PDA, MCM and ME1 medium, we confirm that optimal temperature is 35℃ in MCM and ME1 medium. And optimal media of growth condition is ME1 and MCM.

Many Wild Mushrooms were collected at Mt. Eokbul areas for 2 days in July 2010. and 1 day in August 2010. Identification in the collecting field by taking photos, recording the feature of the surroundings and ecological quality and then observing internal organs through an optical microscope. They were identified and examined with references. According to the result, Species diversities are 2 division, 2 classes, 1 subclass, 5 orders, 17 familys, 30 genera and 37 species. Dominant famillies are Tricholomataceae and Poryporaceae. Dominant species are Trichaptum abietum. Resources of Wild Mushrooms were 5 species in edibility, 6 species in clulture, 8 species in toxine, 6 species in medicne, 4 species in anticancer, 12 species in ectomycorrizahe and 16 species in rotten wood. Therefore the study is aimed at providing fundamental materials required for preserving Mt. Eokbul.

A Lentinula edodes was known to the excellent foods that abundant in nutritious components such as a fiber, minerals and vitamins. Fermentation conditions for rice wine were examined by using the medicinal herbs and the incuvated-rice with mycelia of Lentinula edodes. The average of ethanol contents was in the range of 3.4~4.1% at initial fermentation, increasing up to 16.9% after 15 days. Total acid, pH, reducing sugar and alcohol content were similar to the control during storage periods. The change of colors was measured by Hunter's color value during fermentation. The L value was the highest on 15th day of fermentation.

A Lentinula edodes has abundant nutritious components such as a fiber, minerals and vitamins. It also was known to effective of anticancer and the immune enhancement. However, studies of mushroom-re1ated products were not still enough. Mushroom jerky was examined by using the under grade Lentinula edodes in this study. Content of stipe was most appropriate 24-30% in slices of dried mushroom. A grinder used 2mm sieve, and graund three or four times. A mushroom and spices were boiled to 30min in order to control the microbe occurrence. A mushroom, water, spices were mixed at ratio of 1 : 4: 1.5 and boiled 10 minutes for sterilization. The optimum drying temperature was 60~70℃ but the edge of slice was burned 80~90℃. Dry time was most appropriate for 4~5 hours.

Macrolepiota spp. list of the species found in Korea is composed of 4 species(Lee & Lee 2000). Macrolepiota spp. in Korea is M. alborubescens, M. neomastoidea, M. procera , and M. rhacodes. As the member of Lepiotaceae, Macrolepiota belongs under Eumycotina, Basidiomycotina, Eubasidimycotina, Hymenomycetidiae, and Agaricales. Its outer pileus of Macrolepiota spp. is serrate radially, a small scale is scattered its peridium. And stipe has a small annulus attached to it. Spores are subspore or fusiform, white and it has pseudo-amyloid. Each shape, size, shine and quility of fruiting body of Macrolepiota spp. is different in the breed and it is important sources of a classification of Macrolepiota spp. Especially, the shapes and colors of various scaly which are on the surface of the fungus are important to the external classification.

As a result of analysis of Jangaji used to jangheung oak-mushroom, water content appeared to young oakmushroom 83.13%, completely mature oak-mushroom 66.82%. As the harvest times are delay, water content decreased. As a oak-mushroom was mature, crude protein content increased. But it was decreased after completely maturing. The crude fat content in the pileus part 10-20 times were higher than a stalk parts. As a oak-mushroom grew, content of crude fiber increased in both a pileus and a stalk. The reducing sugar content in the pileus part 10-20 times were higher than stalk parts. After it was 2~4 hours preserve with salt, salinity appeared to proper density at 2.69~2.52%. The most suitable water content appeared after 4 hours drying in 40℃ in order to improve a taste and incense. It was longer preserve with salt, brightness was became lower in both jangaji and spices. When 1.5 month of acceleration experiment compared with room temperature three months, microbe inspection was stationary. Therefore, We fixed expiry date at 12 months considering stability of a product.

The change on quality was measured to development functional steak sauce using superior oak-mushrooms with unique scent and functionality. Measure for Chromaticity according to method of addition indicated that L value of Experimental treatmentⅠ(oak-mushroom powder 1%, chopping 3%) was lower than Control and Experimental treatmentⅡ(Concentrated liquid 0.5%, chopping 3%). There was no significant difference between Control and two Experimental treatments in the result of pH measure. The total acidity showed that there was no significant difference between Experimental treatmentⅠ(1.544%) and Experimental treatmentⅡ (1.538%), but Experimental treatmentⅠand Ⅱ were lower than Control. The quantitative analysis of Chlorine showed that there was no significant difference among Experimental treatmentⅠ(4.19W/V%), Experimental treatmentⅡ(4.32W/V%) and Control(4.23W/V%). The viscosity on Experimental treatmentⅠand Ⅱ were more increased than Control. Microorganism was not discovered in the result of microorganism experiment of oakmushroom sauce. Experimental treatmentⅠwas higher preference than Experimental treatment Ⅱ.

We used the linteus and oak-mushroom fungus that were a functionality mushroom, and We developed Korean cookies. Study of Korean cookies development used functionality mushroom mycelium culture rice. The optimal Proper water for culture rice were on 4 hours ideal. The optimal temperature for oak-mushroom mycelial growth of 25℃. The grain inoculum-source was the best in the oak-mushroom. And the lipid inoculum-source was the best in the linteus . Crude protein, crude fat, crude fiber appeared high at general component analysis results culture rice. but difference didn’t happen in crude ash. This culture rice of mushroom fungus contained glucose, sucrose in free sugar of the analysis results. Glucoses appeared high 10-50 degree compare with control at culture rice. This culture rice of mushroom fungus contained oxalic acid, tartaric acid, malic acid in total organic acid of the analysis results. Total organic acid appeared high compare with control at culture rice. K was detected highest during mineral element. Total mineral element content appeared high at linteus (411.86mg%) and oak-mushroom(410.10mg%) culture rice than control(220.80mg%). As a result of having measured chromaticity of culture rice, a L value and a(red color) value appeared high except red rice. Also, b(yellow color) value appeared high at linteus black rice than control. Sensuality evaluation results of the Korean cookies which used culture rice showed for the preference that control was higher.

This study was performed to analysis of chemical constituent in Polygonum multiflorum root (PMR) by different dry methods (hot-air dry, shade dry, and freeze dry). The results are summarized as followings; major free sugar were detected fructose, glucose, and sucrose in dried PMR based on various dry methods. The highest content of free sugars was found in freeze dried PMR. The four organic acids were detected in dried PMR by HPLC analysis. The content of oxalic acid in shade dried PMR was higher than the dried PMR by different dry methods. The content of total amino acid and essential amino acids were high in the orders of freeze drying > shade drying > hot-air drying. The potassium and magnesium levels of freeze dried PMR was significantly higher than the other drying method of PMR. Whereas the calcium and sodium levels were higher in hot-air dried PMR. The major fatty acids were determined the linoleic acid in PMR by different dry methods.

In this study, we investigated the variation in free sugars, organic acids, antioxidant activity and anti-inflammatory effect of Solanum nigrum Linne fruits according to harvest time. Four kinds of free sugars (fructose, glucose, sucrose, maltose) were detected in S. nigrum fruit, and the free sugar contents varied significantly with harvest time. Organic acid content of S. nigrum fruit showed the highest in malic acid and acetic acid, and the highest content of total organic acids was found in S. nigrum fruit harvested on October 18th and October 25th. For the total polyphenol content, S. nigrum fruit harvested on October 18th was the highest. The strongest DPPH and ABTS radical scavenging activity was showed in S. nigrum fruit harvested on October 11th and October 18th. The anti-inflammatory activity and antioxidant effects were the highest in the ethanol extract from S. nigrum fruit collected on October 18th and October 11th. Thus, it seems the best to harvest of S. nigrum fruit harvested on October 11th and October 18th.

In this study, we investigated the variation in free sugars, amino acids, antioxidant activity, and anti-inflammatory activity of Solanum nigrum Linne based on harvest time. Major amino acids identified by HPLC analysis were proline, histidine, and serine. The highest content of total amino acids were found in S. nigrum aerial parts and roots harvested on July 10th and August 10th. Four kinds of free sugars (fructose, glucose, sucrose, maltose) were detected in S. nigrum, and the free sugar content varied significantly with harvest time. The fructose content of S. nigrum decreased with as harvest time increased. The total polyphenol content of S. nigrum was highest in those harvested on August 30th. The antioxidant activity of ethanol extract from S. nigrum collected at different harvest times were measured by DPPH and ABTS radical scavenging assays. The anti-inflammatory activity of these extracts were assayed via nitric oxide suppression in C6 glioma cells with a lipopolysaccharide (LPS)-induced inflammatory response. The anti-inflammatory activity and antioxidant effects were the highest in the extract from S. nigrum collected on August 30th. Good correlations were observed between antioxidant and anti-inflammatory activities in ethanol extract of S. nigrum roots harvested on August 30th.