This study describes two cases of urolithiasis-related mortality in Asian small-clawed otters (Aonyx cinerea) raised together for over 10 years. Case 1 had multiple renal and bladder stones without clinical signs and died during surgery for stone removal. Case 2, which harbored more extensive renal calculi, also showed no clinical signs but died suddenly from hydronephrosis and renal failure caused by bilateral obstruction of the renal pelvis. In both cases, the uroliths were composed of calcium oxalate. These results highlight the importance of regular examinations in captive otters.
The global e-waste problem is becoming increasingly serious. China, as one of the largest producers and consumers of electronic products, still has a low formal recycling rate. Consumers, as the owners of waste electronics, are the key to successful reverse logistics. However, many choose to store or dispose of e-waste at home rather than use official recycling channels. While many previous studies focus on factors that encourage recycling, fewer examine what stops people from taking part. This study applies Valence Theory to identify the factors that increase consumers’ psychological resistance to recycling small e-waste in China’s first-tier cities. It also examines how these factors influence social value and resistance behavior. The research model includes perceived price unfairness, perceived inconvenience, perceived benefits, and information publicity, with social value as a mediator. Data were collected through an online survey of 303 residents in Beijing, Shanghai, Guangzhou, and Shenzhen. Structural equation modeling (SEM) was used for analysis. The results show that perceived inconvenience and perceived benefits significantly influence social value. Perceived price unfairness, perceived inconvenience, and social value significantly affect consumer resistance. These findings expand the application of Valence Theory in e-waste research and address gaps in the Theory of Planned Behavior by considering both perceived risks and benefits. Practically, this study suggests that manufacturers, recyclers, and policymakers should improve recycling facilities, make the process more convenient, ensure fair and transparent pricing, and create targeted measures to reduce consumer resistance and encourage participation in formal recycling systems.
The commercial feed additive, native rumen microbes (RC), derived from a diverse microbial community isolated from the rumen of Hanwoo steers is being explored to enhance rumen fermentation and improve ruminant feed utilization. This study evaluated the impact of native rumen microbes supplementation on methane emissions, microbial diversity, and fermentation efficiency on in vitro assessment. Treatments were as follows: CON (basal diet, without RC); T1 (basal diet + 0.1% RC); T2 (basal diet + 0.2% RC). Rumen fermentation parameters, total gas, and methane production were assessed at 12, 24, and 48 h of incubations. The in vitro gas production was carried out using the Ankom RF Gas Production System. Supplementation of RC significantly reduced the total gas production at 12, 24, and 48 hours of incubation (p < 0.05). Volatile fatty acid concentrations were increased, while acetate and propionate were decreased (p < 0.05) at 48 h by the supplementation of RC. Notably, the 0.1% inclusion level of RC significantly reduced methane production by 28.30% and 21.21% at 12 and 24 hours. Furthermore, microbial diversity analysis revealed significant shifts (p < 0.05) in bacterial composition between the control and treatment groups, while supplementation also promoted the growth of bacterial populations, such as Succiniclasticum. These findings suggest that native rumen microbes supplementation, particularly at 0.1% inclusion level, can enhance rumen microbial composition while significantly reducing methane production in vitro.
This study investigated the flowering response of three Korean native Aster species, namely A. hayatae, A. spathulifolius, and A. koraiensis, to varying photoperiods. Three-month-old plants propagated from cuttings were grown under four different photoperiods: 9, 12, 14, and 16 h. Aster hayatae flowered under all conditions, with flowering rates of 92%, 85%, 65%, and 27% under 9-, 12-, 14-, and 16-h photoperiods, respectively. Flowering in A. hayatae was promoted by shorter photoperiods, classifying it as a facultative short-day plant. Aster spathulifolius flowered only under 9- and 12-h photoperiods, with no significant difference between these treatments, suggesting that the species is an obligate short-day plant. However, given the low A. spathulifolius flowering rates of 27% and 13% under 9- and 12-h photoperiods, respectively, further research is required. Aster koraiensis did not flower under any photoperiod, possibly due to vernalization requirements or juvenility. These findings offer valuable insights into the photoperiodic flowering responses of these three Korean native Aster species, enhancing our understanding of their ecological traits and potential horticultural applications.
본 논문은 라이소자임이 칸디다 알비칸스에 항균효능이 있음을 발견하였고 라이소자임의 분해 절편도 또한 항균효능이 있는지 단백질 분해 효소인 트립신을 처리하여 확인해 보았다. 130개의 아미노산 으로 구성된 14 kDa 단백질인 라이소자임을 약 24 kDa의 분자량을 가지는 단백질 분해 효소인 트립신으 로 분해 하였다. 분해된 생성물은 고성능 액체 크로마토그래피인 분석용 및 제조용 HPLC를 사용하여 분 석 및 분리하였다. 이를 통해 효율적인 라이소자임 소화를 위한 최적 조건을 확인하고, 분해된 펩타이드 절 편들을 분리하였다. 효능평가 결과, 일부 펩타이드 절편들이 칸디다 알비칸스에 강한 항균 활성을 보임을 확인하였으며 몇몇은 활성이 줄어드는 결과를 보였다.
Ectopic ureter refers to a congenital anomaly in which one or both ureters do not connect to the urinary bladder at the correct anatomical site. This case report discusses the case of a 6-year-old female mixed-breed dog diagnosed with chronic urinary incontinence, systemic hypertension, pancreatitis, and sepsis resulting from an ectopic ureter. Treatment involved an initial nephro-ureterectomy to address severe pyonephrosis, followed by ureteroneocystostomy for the remaining functional kidney. Post-surgical outcomes showed notable improvements in clinical symptoms, laboratory findings, and blood pressure. This report emphasizes the need for early diagnosis and appropriate surgical treatment in cases of ectopic ureter. Additionally, it aims to present the clinical symptoms and conditions resulting from prolonged disease progression, as well as the corresponding treatment methods and prognosis.