CANDU Spent Fuel (CSF) dry storage system, SILO, has been operated from 1992 at Wolsung under 50 year operating license. As of 2023, this system has been operated for over 30 years and its licensed remaining operation time is less than 20 years. When it faces the final stage of operation, it has only two options; moving to a centralized away-from-reactor storage or extending its license atreactor. These two options have an inevitable common duty of confirming the CSF integrity by a “demonstration test”. Since the degradation of CSF and structural materials in the SILO are critically dependent on temperature, two important goals of the ‘DEMO test’ were set as follows. 1. Design of ‘DEMO SILO’: Development of internal monitoring technology by transforming SILO design. 2. Accurate measurement and evaluation of the three-dimensional temperature distribution in the ‘DEMO SILO’ Based on operating real commercial SILO dimension, a conceptual “DEMO SILO” design has been developed from 2022. Because, unlike with commercial Silo, ‘Demo Silo’ must be disassembled and assembled, and have penetration holes. Safety evaluation technologies like structural, thermal and radiation protection analysis also have been developed with design work. ‘Demo SILO’ should evaluate an accurate 3D temperature distribution with minimal number of thermocouples and penetration holes to avoid disruption of internal flow and temperature distribution. For this reason, a ‘Best Estimate Thermal-Hydraulics evaluation system for SILO’ is under development and it will be essential for ensuring temperature prediction accuracy. Construction of a full-scale test apparatus to validate this technology will begin in 2024. In order to supply power to many heaters and monitor temperature gradient inside of this apparatus, it has modular design concept by dividing its whole body to axial 9 sub-bodies which looks like a donut containing a basket at center position.

수송은 양식어류의 양식과정에서 필수적으로 수행되며, 선별과 같은 물리적인 스트레스 요인 중 하나이다. 이러한 이유로 본 연구에서는 해산어류인 넙치를 이용하여 수송 시 염분, 수온 및 마취제의 영향을 확인하기 위해 수송 시 해수 염분(35‰, 15‰)과 수온은 20℃ (natural water temperature, NWT), 15℃ (cooling water temperature, CWT)로 설정하였으며 마취제(anesthesia, Anes., Sigma USA)는 50 ppm 희석하여 염분, 수온, 마취제 요인들을 혼합한 실험구를 설정하였 다. 실험구는 각각 NWT+35‰, CWT+ 35‰, NWT+15‰, CWT+15‰, NWT+15‰+Anes. 및 CWT+15‰+Anes.으로 설정하였다. 스티로폼 상자(66×42×20 cm)를 수송용기로 사용하였으 며, 해수 3 ℓ와 액화산소를 주입한 비닐봉지에 넙치 8마리씩 수용하여 수송하였다. 연구 결과 수송 전 코티졸 농도는 2.4±0.1 ng ml-1로부터 CWT+35‰ 구(16.7±12.8 ng ml-1)를 제외한 나 머지 실험구에서 유의하게 높아졌다. K+ 농도는 수송 전 3.1±0.0 mEq l-1로부터 NWT+15‰ 구에서 4.5±1.1 mEq l-1로 차이를 보이지 않았으나, 나머지 실험구에서는 모두 유의하게 높은 값을 보였다. 혈액성상의 변화에는 영향을 미치지 않았으며, 수온과 마취제는 스트레스를 유발 로 인한 삼투압조절에 부정적인 영향을 미쳤다. AST, ALT에는 영향을 미치지 않았다.

The baculovirus expression system is a very useful tool widely used for expression of foreign proteins. To use the baculovirus expression system, a recombinant baculovirus must be prepared. The development of the Bac to Bac system has reduced the time and effort required to produce recombinant baculovirus. But, it will take at least two weeks. Further, it takes more time to measure the activity of recombinant baculovirus. In order to overcome this problem, a virus inducible expression system is being studied recently. Although baculovirus is able to rapidly express foreign proteins, it still has a low expression level. Thus, in this study, we aimed to construct a novel baculovirus inducible expression vector that not only shortens the production time of protein but also can express at a high level. The novel baculovirus inducible expression vector has been evaluated using EGFP and is expected to be a very useful tool for production of various proteins.

A powder injection molding process is developed and optimized for piezoelectric PAN-PZT ceramics. Torque rheometer experiments are conducted to determine the optimal solids loading, and the rheological property of the feedstock is evaluated using a capillary rheometer. Appropriate debinding conditions are chosen using a thermal gravity analyzer, and the debound specimens are sintered using sintering conditions determined in a preliminary investigation. Piezoelectric performance measures, including the piezoelectric charge constant and dielectric constant, are measured to verify the developed process. The average values of the measured piezoelectric charge constant and dielectric constant are 455 pC/N and 1904, respectively. Powder injection molded piezoelectric ceramics produced by the optimized process show adequate piezoelectric performance compared to press-sintered piezoelectric ceramics.

The objective of this study was to evaluate the effects of acute hypoxia on the physiological stress responses (plasma cortisol as the primary response, and hematocrit, hemoglobin, plasma glucose, sodium, chloride, osmolality, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as the secondary responses) of Korean rockfish. The mortality in Korean rockfish started at 0.7 mg L-1 and 0.8 mg L-1 levels at 12℃ and 20℃, respectively. And the time required for the mortality in experimental fish was 274 and 148 minutes at 12℃ and 20℃, respectively. Levels of hematocrit, hemoglobin, AST, ALT, ion concentration, osmolality, glucose and cortisol had significantly increased with decreasing dissolved oxygen at 12℃ and 20℃.

The aim of the present study was to assess the effects of ammonia on physiological responses in Korean rockfish (Sebastes schlegeli). Interestingly, no mortality were observed when the specimens (301.1±8.0 g) were exposed to five levels of un-ionized ammonia (NH3) (control, 1, 2, 4, 8 mg L-1) for 3 hours. Furthermore, a significantly higher increase in gill Na+/K+-ATPase (NKA) pump activity with was detected due to the ammonia exposure. The activity of the fishes were found to be 4 and 8 mg L-1 NH3, which was significantly high compared to normal 1 mg L-1 NH3. Although ammonia exposure had no effect on plasma Cl-, exposure to both 4 and 8 mg L-1 NH3, however it led to increase in the plasma Na+, K+ and osmolality levels. Also, prolong ammonia exposure cause increase of plasma cortisol and glucose levels. The increase in glucose was accompanied by an increase in cortisol. The fish exposed to 4 and 8 mg L-1 NH3 showed significantly higher hematocrit than control group than those exposed to 1 and 2 mg L-1 NH3. The intensity of cell damage increased with the increase concentration and exposure to ammonia. Furthermore, hyperplasia, separation and epithelial necrosis were also observed in gill tissues. Taken together, the results showed that direction of changes to the investigated parameters can be used to determine the physiological responses of Korean rockfish to ammonia.

Most traditional genome sequencing projects involving infectious viruses include culturing and purification of the virus. This can present difficulties as an analysis of multiple populations from multiple locations may be required to acquire sufficient amount of high-quality DNA for sequence analysis. The electrophoretic method provides a strategy whereby the genomic DNA sequences of the Korean isolate of Pieris rapae granulovirus (PiraGV-K) were analyzed by purifying it from host DNA by pulsed-field gel electrophoresis, thus simplifying sampling and labor time. The genomic DNA of infected P. rapae was embedded in agarose plugs, digested with a restriction nuclease and methylase, and pulsed-field gel electrophoresis (PFGE) was used to separate PiraGV-K DNA from the DNA of P. rapae, followed by mapping of fosmid clones of the separated viral DNA. The double-stranded circular genome of PiraGV-K encodes 120 open reading frames (ORFs), covering 92% of the sequenced genome. BLAST and ORF arrangement showed the presence of 78 homologs to other genes in the database. The mean overall amino acid identity of PiraGV-K ORFs was highest with the Chinese isolate of PiraGV (~99%), followed up with Choristoneura occidentalis ORFs at 58%. PiraGV-K ORFs were grouped, according to function, into 10 genes involved in transcription, 11 involved in replication, 25 structural protein genes, and 15 auxiliary genes. Genes for Chitinase (ORF 10) and cathepsin (ORF11), involved in the liquefaction of the host, were found in the genome. The recovery of PiraGV-K DNA genome by pulse-field electrophoretic separation from host genomic DNA had several advantages, compared with its isolation from particles harvested as virions or inclusions from the P. rapae host. We have sequenced and analyzed the 108,658 bp PiraGV-K genome purified by the pulsed field electrophoretic method. The method appears to be applicable to the analysis of genomes of large viruses. The chitinase, identified by PiraGV-K genome sequence, was functionally characterized by quantitative PCR, Western blot analysis, immunohistochemistry and transmission electron microscopy.

In this study, we investigated the growth of striped mullet (Mugil cephalus), Yesso scallop (Patinopecten yessoensis) and kelp (Saccharina japonica) farmed under the IMTA (integrated multi-trophic aquaculture) system developed by national institute of fisheries science (NIFS). The farmed striped mullets grew from an initial length and weight of 152.5±12.1 and 41.6±7.8 g in October 2013 to 154.2±5.6 and 47.5±8.6 g in November, 160.2±8.7 and 55.9±9.1 g in December and 168.4±9.6 and 58.4±8.7 g in January. The fish continued to grow and reached 190.2±9.4 in length and 87.5±8.9 g in weight in April and 256.4±9.7 and 156.7±6.7 g in October 2014. The daily growth rate (DGR) for total fish length was 0.015 0.1 /day during the periods of fast growth and attained 0.038 0.1 /day during February March. The kelp grew from an initial blade length and wet weight of 1.19±0.2 and 0.0028±0.0012 g in January 2014 to 3.3±0.8 and 2.5±0.9 g in February and 126.5±11.6 and 107.4±22.6 g in March, after which, erosion occurred and slowed the growth. The DGRs for kelp length ranged 0.03 1.9 /day in January 2014 and increased to 0.88 1.9 /day during March April. Increasing water temperatures beginning in April lowered the DGR to 0.03 /day. Yesso scallops grew from an initial shell length, shell height and wet weight of 11.83±0.6 , 12.68±0.7 and in September 2013 to 19.9±2.5 , 20.8±2.6 and 0.9±0.04 g in November 2013. They continued to grow to 45.91±0.71 in shell length, 42.55±0.8 in shell height and 12.7±1.3 g in wet weight by May 2014 and 60.2±2.51 , 554.6±2.61 and 24±2.70 g by October 2014. The DGRs for shell length of Yesso scallop ranged from 0.02 to 0.256 /day with higher values of 0.256 0.27 /day during November December 2013 and March April 2014.