In Korea, the agricultural industry has witnessed a growing emphasis on reducing reliance on imported forage by adopting locally available alternatives to enhance sustainability and self-sufficiency. Given the limited information on the potential use of whole-crop rice silage (WCRS) as livestock feed, this study evaluated the effects of total mixed rations (TMR) containing WCRS on the rumen microbiota of Hanwoo heifers. Thirty heifers (body weight = 351 ± 39 kg) were randomly assigned to three TMR diet groups for the early fattening period: oat hay (OH), oat hay with Italian ryegrass silage and corn silage (OIC), and silages from whole-crop rice, Italian ryegrass, and corn (WCRSEF). During the late fattening period, the same heifers (569 ± 40 kg) were reassigned to three other treatment groups: perennial ryegrass (PRG), Italian ryegrass silage and alfalfa hay (IRGA), and silage containing whole-crop rice (WCRSLF). The effects of different feeding diets on the rumen microbial composition of Hanwoo heifers were investigated using high-throughput 16S rRNA gene sequencing. The analysis revealed similar microbial diversity among the treatments across both fattening periods. Bacteroidetes and Firmicutes were the most dominant phyla during early and late fattening periods. Moreover, during the early fattening period, heifers fed WCRS exhibited a higher ratio of Bacteroidetes to Firmicutes, whereas Firmicutes became more predominant in the late fattening period. Hungateiclostridium and Porphyromonas were identified as biomarkers (LDA score > | 2 |; p < 0.05) for the WCRSEF and WCRSLF groups, respectively. Furthermore, the microbial co-occurrence network highlighted different patterns (| r | = 0.85; p < 0.05) between the early and late fattening periods. The results provide significant insights into the effects of WCRS as a substitute for conventional forages such as oat hay, perennial ryegrass, alfalfa, and Italian ryegrass silage. The findings suggest that WCRS can modulate the rumen microbiome of Hanwoo heifers.

This study aims to examine how the body and clothing of Jesus Christ are represented in the Russian Transfiguration of Christ icons of the 15th and 16th centuries and why it is considered to have established a distinct Russian iconography free from Byzantine influence. The study analyzes the Russian icons comparing them with their Byzantine prototypes to identify the distinctive ‘Russianness’ of the representation. The research methods comprise a qualitative analysis of the literature on Russian Orthodoxy, Russian icons, Christian theology, and Christ’s clothing and an empirical analysis of the icons. The scope of the study is limited to Russian icons from the 15th and 16th centuries and Byzantine icons from the 9th century onwards. The study found that, compared to Byzantine icons, the Russian icons exhibit a more elongated body, darker facial features, and lighter clothing. A statistical analysis using the Mann-Whitney U test further revealed that the Russian icons feature a stiffer, simpler form, more opaque material, and more defined clothing boundaries. These stylistic differences suggest that the ‘Russianness’ in the expression of Christ’s body and clothing in the Transfiguration of Christ icons derives from creativity, abstractness, and tradition. Rather than a simple recreation of the historical Christ or adherence to the Byzantine tradition, the Russian iconographic representation emphasizes Christ's transfiguration into a luminous form, as described in the Bible, accentuating his divine nature over his human aspects.

This study was conducted to report the census survey results of south Korean Chikso (Korean Brindle Cattle) distribution and to diagnose the current coat color expression patterns. Two years Chikso census showed that there were 2,413 Chikso in 2013 and 2,754 in 2014 in south Korea. Number of animals between 0 to 35 months of age was 1,632 heads in 2014 which comprised 59% of total Chikso population size. The percentages of animals between 36 ~ 71 months of age and over 6 years of age were 31% and 10% of total. Out of 2,416 animals with class numbers assessed by their coat colors, 1,249 heads (52%) were in group I (with black stripes, class no. 1 ~ 3) and the others (class no. 4 ~ 7) were in group II (1,167 heads, 48%). Among the 1,551 animals that were photographed twice in 2013 and in 2014, 226 animals were assessed different coat color class numbers, and round 90% of which were within the ages less than 48 months. The number of animals switched in coat color pattern groups over a year was 56, which was 3.6% of total number of animals on survey. And around 88% of the animals switched in group category was of the animals younger than 24 months of age. Therefore, we conclude that the coat color pattern becomes rather stable at around 24 months of age in Chikso populaion in south Korea.

이탈리안 라이그라스(Lolium multiflorum Lam., IRG)는 주로 답리작으로 재배되고 있으며 파종기는 벼 수확시기와 겹치고 파종기에 강우에 의하여 파종 적기를 놓칠 수 있 다. 따라서 본 연구는 이탈리안 라이그라스의 적기 파종을 놓쳤을 때 봄 파종시 수량성 및 사료가치에 미치는 영향을 검토하고자 2012월 10월부터 2013년 6월까지 전라남도 강 진에서 수행하였다. 파종시기를 주구로 2월 15일, 2월 25 일, 3월 5일, 3월 15일로 4처리 하였으며, 품종을 세 구로 극조생종 그린팜(Greenfarm), 조생종 코윈어리(Kowinearly) 그리고 중생종 코윈마스터(Kiwinmaster)를 공시하였다. 이탈리안 라이그라스의 파종시기별 출수기는 2월 15일 파종에서는 Kowinmaster, Kowinearly 및 Greenfarm은 각각 5월 14일, 5월 12일, 5월 9일로 극조생종과 중생종간의 차 이는 5일의 숙기 차이가 있었으나, 3월 15일 파종에서는 각각 5월 23일, 5월 22일, 5월 20일로 극조생종과 조생종 간의 생육차이는 3일의 숙기 차이가 나타났다. 그리고 초 장은 Kowinmaster를 2월 15일 파종하였을 때 105.7 cm로 3 월 15일에 파종하였을 93.0 cm 보다 길었으며, Kowinearly 와 Greenfarm 품종도 2월 15일과 2월 25일 파종구에서 초 장은 비슷하였으나, 3월 5일과 3월 15일에서는 짧았다. 그 리고 도복은 빨리 파종하였을 때 심했으며, 품종간의 차이 는 적었다. 그리고 건물수량은 Kowinearly 조생종을 2월 15일에 파 종하여 5월 30일에 수확할 때 7,333 kg/ha으로 가장 많았 고, Green farm 극조생종에서 6,609 kg/ha으로 적었으며, 유 의적 차이는 없었다. 그러나 파종시기별 건물수량은 2월 15일에 파종했을 때 7,052 kg/ha으로 3월 15일 파종구의 4,698 kg/ha 보다 유의적으로 많았다(P<0.05). 조단백질의 경우 2월 15일 파종에서 평균 8.8%인데 반해 3월 15일 파 종에서 평균 11.1%로 약 2.3% 높게 나타났으며, ADF는 2.1% 낮게 나타났고 TDN도 1.5% 정도 높았다. 그리고 RFV에서는 5.9% 정도 높아 파종시기가 늦어짐에 따라 사 료가치는 좋았으며 품종간의 차이는 적었다. 이상의 결과를 종합적으로 고려할 때 남부지역 논에서 이탈리안 라이그라스의 봄 파종 적기는 2월 25일경이며, 최소한 2월말까지는 파종해야 수량성과 사료가치가 높은 조사료(forage)를 생산할 수 있을 것으로 사료된다.

It has been reported that light-emitting diodes(LED) can be used in the treatment of oral diseases. Although bio-stimulatory effects of LED irradiation such as promotes stimulation of wound healing have been well known, there are few reports about molecular mechanism associated with cell cycle by LED irradiation. The purpose of present study was to examine the molecular event in cell cycle of LED irradiation on primary human gingival fibroblast(hGF) in vitro. The source of light for irradiation was a continuous-wave LED emitting at a wavelength of 635nm, and manufactured that energy density was 5mW/cm2 on sample surface. The hGF were irradiated for 1 hour at 37℃ in 5% CO2 humidified chamber. Experimental samples were acquired at 0 (right after irradiation), 8 and 24 hour after irradiation. To investigate the molecular mechanisms associated with cell cycle, growth phase was determined by flow cytometry and mRNA expression of cyclin A, cyclin B, cyclin D1, cyclin E, cdc2, PCNA, p18, p27, p21, and p53 were determined by real time RT-PCR. Flow cytometric analysis demonstrated the percentage of cells in the G1 and S phase were decreased, but the G2 phase increased, which showed cells irradiated by LED were transitioned from S to G2 phase. For mRNA expression, cyclin B, cdc2, PCNA and p53 were increased at 0 hour after irradiation, and most of cell cycle molecules were increased at 8 hour after irradiation. At 24 hour after irradiation, cyclin A, cyclin E, PCNA and p18 were increased. Taken together, LED irradiation induced proliferation of hGF cells through transition from S to G2 phase.

The purpose 01' pl'esent study was to examine the molecular events in apoptosis by CoCl2, mimicking hypoxic cond ition and recovering effects by LED ir l'adiation on Human SH-SY5Y neuroblastoma cells The SOUl'ce 0 1' light for ir l'adiation was a continuous-wave LED emitting at a wavelenl양h of 590 nm, and manufactured that ene rgy density was 5 mW!cm2 on sample surface, After ir l'adiation, cell viabi lity was measured with BrdU , cell morphol ogy was examined with Diff- Quik staining, cell signaling was monitored with various apoptosis-related molecules using RNase Pl'otection Assay(RPA) , W11en treated with CoC12, apoptotic induction was found in the SH-SY5Y cells in a concentration-dependent and time-dependent manner , Diff-Quik s taining was revealed that DNA fragmentation re presented apoptosis was examined in CoC12-tl'eated group, Moreover, RPA assay of SH-SY5Y cclls lIs ing val'iolls apoptosis-related molecllles showed that the apoptotic cell population was mcreased J-loweve. there was sorne signifïcant change in LED irradiatied cells aftel' treatement of CoC12 The main mechanism for Lhese a poptosis appearecl to be mito c hondriεt - m ecliated pathway, such as cytochrome- c‘ caspase-9, caspase-3, pro-apototic protein ßax, anti-apototic protein Bcl-2, and death receptor• mediated pathway, such as Fas, cas pase- 8, a ncl TNFRl These results demonstrate that CoCI2 induce apoptosis in SH-SY5Y via different dual apop tosis pathway through death receptor pathway as well as mitochondria- dependent pathway and LED irradiation can recl llces the CoCl2-induced apoptosis by blocking their internal signaling pathway